LOCUS       62056_12595        5166 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5166
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(399..1772)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
                     LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
                     HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
                     ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
                     MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
                     GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
                     "
     CDS             complement(1982..2983)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     promoter        3480..3584
                     /label=AmpR promoter
     CDS             3585..4442
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4610..5154
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 aagctttgat aggtggtatg ttttcgcttg aacttttaaa tacagccatt gaacatacgg
       61 ttgatttaat aactgacaaa catcaccctc ttgctaaagc ggccaaggac gctgccgccg
      121 gggctgtttg cgtttttgcc gtgatttcgt gtatcattgg tttacttatt tttttgccaa
      181 agctgtaatg gctgaaaatt cttacattta ttttacattt ttagaaatgg gcgtgaaaaa
      241 aagcgcgcga ttatgtaaaa tataaagtga tagcggtacc attataggta agagaggaac
      301 gtacacagat ctgcaggtcg acggatcccc gggaattcct gttataaaaa aaggatcaat
      361 tttgaactct ctcccaaagt tgatccctta acgatttaga aatccctttg agaatgttta
      421 tatacattca aggtaaccag ccaactaatg acaatgattc ctgaaaaaag taataacaaa
      481 ttactataca gataagttga ctgatcaact tccataggta acaacctttg atcaagtaag
      541 ggtatggata ataaaccacc tacaattgca atacctgttc cctctgataa aaagctggta
      601 aagttaagca aactcattcc agcaccagct tcctgctgtt tcaagctact tgaaacaatt
      661 gttgatataa ctgttttggt gaacgaaagc ccacctaaaa caaatacgat tataattgtc
      721 atgaaccatg atgttgtttc taaaagaaag gaagcagtta aaaagctaac agaaagaaat
      781 gtaactccga tgtttaacac gtataaagga cctcttctat caacaagtat cccaccaatg
      841 tagccgaaaa taatgacact cattgttcca gggaaaataa ttacacttcc gatttcggca
      901 gtacttagct ggtgaacatc tttcatcata taaggaacca tagagacaaa ccctgctact
      961 gttccaaata taattccccc acaaagaact ccaatcataa aaggtatatt tttccctaat
     1021 ccgggatcaa caaaaggatc tgttactttc ctgatatgtt ttacaaatat caggaatgac
     1081 agcacgctaa cgataagaaa agaaatgcta tatgatgttg taaacaacat aaaaaataca
     1141 atgcctacag acattagtat aattcctttg atatcaaaat gaccttttat ccttacttct
     1201 ttctttaata atttcataag aaacggaaca gtgataattg ttatcatagg aatgagtaga
     1261 agataggacc aatgaatata atgggctatc attccaccaa tcgctggacc gactccttct
     1321 cccatggcta ctatcgatcc aataagacca aatgctttac ccctattttc ctttggaata
     1381 tagcgcgcaa ctacaaccat tacgagtgct ggaaatgcag ctgcaccagc cccttgaata
     1441 aaacgagcca taataagtaa ggaaaagaaa gaatggccaa caaacccaat taccgacccg
     1501 aaacaattta ttataattcc aaataggagt aaccttttga tgcctaattg atcagatagc
     1561 tttccatata cagctgttcc aatggaaaag gttaacataa aggctgtgtt cacccagttt
     1621 gtactcgcag gtggtttatt aaaatcattt gcaatatcag gtaatgagac gttcaaaacc
     1681 atttcattta atacgctaaa aaaagataaa atgcaaagcc aaattaaaat ttggttgtgt
     1741 cgtaaattcg attgtgaata ggatgtattc acatttcacc ctccaataat gagggcagac
     1801 gtagtttata gggttaatga tacgcttccc tcttttaatt gaaccctgtt acattcatta
     1861 ttcattacac ttcataatta attcctccta aacttgatta aaacatttta ccacatataa
     1921 actaagtttt aaattcagta tttcatcact tatacaacaa tatggcccgt ttgttgaact
     1981 actctttaat aaaataattt ttccgttccc aattccacat tgcaataata gaaaatccat
     2041 cttcatcggc tttttcgtca tcatctgtat gaatcaaatc gccttcttct gtgtcatcaa
     2101 ggtttaattt tttatgtatt tcttttaaca aaccaccata ggagattaac cttttacggt
     2161 gtaaaccttc ctccaaatca gacaaacgtt tcaaattctt ttcttcatca tcggtcataa
     2221 aatccgtatc ctttacagga tattttgcag tttcgtcaat tgccgattgt atatccgatt
     2281 tatatttatt tttcggtcga atcatttgaa cttttacatt tggatcatag tctaatttca
     2341 ttgccttttt ccaaaattga atccattgtt tttgattcac gtagttttct gtattcttaa
     2401 aataagttgg ttccacacat accaatacat gcatgtgctg attataagaa ttatctttat
     2461 tatttattgt cacttccgtt gcacgcataa aaccaacaag atttttatta atttttttat
     2521 attgcatcat tcggcgaaat ccttgagcca tatctgacaa actcttattt aattcttcgc
     2581 catcataaac atttttaact gttaatgtga gaaacaacca acgaactgtt ggcttttgtt
     2641 taataacttc agcaacaacc ttttgtgact gaatgccatg tttcattgct ctcctccagt
     2701 tgcacattgg acaaagcctg gatttacaaa accacactcg atacaacttt ctttcgcctg
     2761 tttcacgatt ttgtttatac tctaatattt cagcacaatc ttttactctt tcagcctttt
     2821 taaattcaag aatatgcaga agttcaaagt aatcaacatt agcgattttc ttttctctcc
     2881 atggtctcac ttttccactt tttgtcttgt ccactaaaac ccttgatttt tcatctgaat
     2941 aaatgctact attaggacac ataatattaa aagaaacccc catctattta gttatttgtt
     3001 tggtcactta taactttaac agatggggtt tttctgtgca accaatttta agggttttcc
     3061 aatactttaa aacacataca taccaacact tcaacgcacc tttcagcaac taaaataaaa
     3121 atgacgttat ttctatatgt atcaagataa gaaagaacaa gttcaaaacc atcaaaaaaa
     3181 gacacctttt caggtgcttt ttttatttta taaactcatt ccctgatctc gacttcgttc
     3241 tttttttacc tctcggttat gagttagttc aaattcgttc tttttaggtt ctaaatcgtg
     3301 tttttcttgg aattgtgctg ttttatcctt taccttgtct acaaacccct taaaaacgtt
     3361 tttaaaggct tttaagcgtc tgtacgttcc ttaaggaatt attccttagt gctttctagg
     3421 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtcc
     3481 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3541 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3601 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     3661 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     3721 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     3781 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
     3841 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     3901 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     3961 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4021 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4081 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa
     4141 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4201 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4261 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4321 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4381 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4441 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4501 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4561 gtgagttttc gttccactga gcgtcagacc ccttaataag atgatcttct tgagatcgtt
     4621 ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag ggaggttttt
     4681 cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgca ggagcgcagt
     4741 caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag actaactcct
     4801 ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc cgggttggac
     4861 tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg ttcgtgcata
     4921 cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg aatgagacaa
     4981 acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag gagagcgcac
     5041 gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacca
     5101 ctgatttgag cgtcagattt cgtgatgctt gtcagggggc ggagcctatg gaaaaacgct
     5161 ttgccc
//
