LOCUS       Exported                2980 bp DNA     circular SYN 11-SEP-2025
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   11111111
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2025 from SnapGene 6.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        45..248
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        293..311
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      343..376
                     /label=murine terminator
     promoter        complement(395..616)
                     /label=human pol I promoter
     polyA_signal    654..878
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1137..1725)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1896..2756)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2757..2861)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
       61 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      121 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      181 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      241 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
      301 ctcactatag ggagacccaa gctgttaacg ctagcagtta accggagtac tggtcgacct
      361 ccgaagttgg gggggaggag acggtaccgt ctccaataac ccggcggccc aaaatgccga
      421 ctcggagcga aagatatacc tcccccgggg ccgggaggtc gcgtcaccga ccacgccgcc
      481 ggcccaggcg acgcgcgaca cggacacctg tccccaaaaa cgccaccatc gcagccacac
      541 acggagcgcc cggggccctc tggtcaaccc caggacacac gcgggagcag cgccgggccg
      601 gggacgccct cccggcggtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
      661 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      721 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      781 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
      841 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
      901 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      961 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     1021 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     1081 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     1141 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     1201 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     1261 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     1321 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     1381 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     1441 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     1501 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     1561 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     1621 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     1681 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1741 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1801 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1861 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1921 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1981 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     2041 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     2101 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     2161 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     2221 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     2281 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     2341 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     2401 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     2461 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     2521 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     2581 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     2641 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     2701 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2761 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2821 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2881 ccacctgacg tcgatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     2941 gcctggcatt atgcccagta catgacctta tgggactttc
//
