LOCUS       V005476                 4664 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005476
VERSION     V005476
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Yang H, Yang Z.
  TITLE     A novel way overcoming blunt-ligation in the construction of
            Streptomyces conjugative vector pHW001
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4664)
  AUTHORS   Yang H, Yang Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2006) Life Science and Technology Department,
            Biochemistry, Huazhong Agricultural University, 1 Shizishan Street,
            Wuhan, Hubei Province 430070, China
REFERENCE   3  (bases 1 to 4664)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4664)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2006) Life Science and Technology Department, Biochemistry,
            Huazhong Agricultural University, 1 Shizishan Street, Wuhan, Hubei
            Province 430070, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4664
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(399..455)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     oriT            610..719
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             752..1120
                     /label="traJ"
                     /note="oriT-recognizing protein"
     CDS             complement(1410..2216)
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     regulatory      complement(2267..2272)
                     /gene="tsr"
                     /label="tandem promoter tsrp1"
                     /note="tandem promoter tsrp1"
                     /regulatory_class="minus_10_signal"
     regulatory      complement(2395..2400)
                     /gene="tsr"
                     /label="tandem promoter tsrp2"
                     /note="tandem promoter tsrp2"
                     /regulatory_class="minus_10_signal"
     primer_bind     complement(2443..2459)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2467..2483)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2491..2521)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2536..2557)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2845..3433)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3607..4464)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(4465..4569)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcatgc ctgcaggtcg
      421 actctagagg atccccgggt accgagctcg aattcccaat gcaggtcgac ggatcttttc
      481 cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt
      541 cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg
      601 gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac
      661 tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccggc
      721 taccgccggc gtaacagatg agggcaagcg gatggctgat gaaaccaagc caaccaggaa
      781 gggcagccca cctatcaagg tgtactgcct tccagacgaa cgaagagcga ttgaggaaaa
      841 ggcggcggcg gccggcatga gcctgtcggc ctacctgctg gccgtcggcc agggctacaa
      901 aatcacgggc gtcgtggact atgagcacgt ccgcgagctg gcccgcatca atggcgacct
      961 gggccgcctg ggcggcctgc tgaaactctg gctcaccgac gacccgcgca cggcgcggtt
     1021 cggtgatgcc acgatcctcg ccctgctggc gaagatcgaa gagaagcagg acgagcttgg
     1081 caaggtcatg atgggcgtgg tccgcccgag ggcagagcca tgactttttt agccgctaaa
     1141 acggccgggg ggtgcgcgtg attgccaagc acgtccccat gcgctccatc aagaagagcg
     1201 acttcgcgga gctggtgaag tacatcaccg acgagcaagg caagaccgat ccccggggac
     1261 ctgcattggc aattcccaac atgctgcgcc ggttcggggg cggcgagccc gggaggccaa
     1321 tcccgggctc gtgccatttc tgggtcctgt tgatcatcac tgacgaatcg aggtcgagca
     1381 accgagcgtc cgaggaacag aggcgcttat cggttggccg cgagattcct gtcgatcctc
     1441 tcgtgcagcg cgattccgag ggaaacggaa acgttgagag actcggtctg gctcatcatg
     1501 gggatggaaa ccgaggcgga agacgcctcc tcgaacaggt cggaaggccc acccttttcg
     1561 ctgccgaaca gcaaggccag ccgatccgga ttgtccccga gttccttcac ggaaatgtcg
     1621 ccatccgcct tgagcgtcat cagctgcata ccgctgtccc gaatgaaggc gatggcctcc
     1681 tcgcgaccgg agagaacgac gggaagggag aagacgtaac ctcggctggc cctttggaga
     1741 cgccggtccg cgatgctggt gatgtcactg tcgaccagga tgatccccga cgctccgagc
     1801 gcgagcgacg tgcgtactat cgcgccgatg ttcccgacga tcttcacccc gtcgagaacg
     1861 acgacgtccc cacgccggct cgcgatatcg ccgaacctgg ccgggcgagg gacgcgggcg
     1921 atgccgaatg tcttggcctt ccgctccccc ttgaacaact ggttgacgat cgaggagtcg
     1981 atgaggcgga ccggtatgtt ctgccgcccg cacagatcca gcaactcaga tggaaaagga
     2041 ctgctgtcgc tgccgtagac ctcgatgaac tccaccccgg ccgcgatgct gtgcatgagg
     2101 ggctcgacgt cctcgatcaa cgttgtcttt atgttggatc gcgacggctt ggtgacatcg
     2161 atgatccgct gcaccgcggg atcggacgga tttgcgatgg tgtccaactc agtcatggtc
     2221 gtcctaccgg ctgctgtgtt cagtgacgcg attcctgggg tgtgacaccc tacgcgacga
     2281 tggcggatgg ctgccctgac cggcaatcac caacgcaagg ggaagtcgtc gctctctggc
     2341 aaagctcccc gctcttcccc gtccgggacc cgcgcggtcg atccccgcat atgaagtatt
     2401 cgccttgatc ttcgcgtaga gctttggcaa ttcgtaatca tggtcatagc tgtttcctgt
     2461 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     2521 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     2581 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     2641 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     2701 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     2761 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     2821 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     2881 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     2941 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     3001 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     3061 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     3121 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     3181 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     3241 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     3301 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     3361 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     3421 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     3481 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     3541 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     3601 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     3661 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     3721 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     3781 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     3841 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     3901 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     3961 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     4021 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     4081 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     4141 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     4201 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     4261 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     4321 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     4381 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     4441 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     4501 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     4561 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
     4621 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtc
//
