LOCUS       V005478                 5157 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005478
VERSION     V005478
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     A synthetic multicellular system for programmed pattern formation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2005) Electrical Engineering, Princeton
            University, B-312, E-Quad, Olden Street, Princeton, NJ 08544, USA
REFERENCE   3  (bases 1 to 5157)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5157)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (25-JAN-2005) Electrical Engineering, Princeton University, B-312,
            E-Quad, Olden Street, Princeton, NJ 08544, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5157
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(125..1204)
                     /label="lacI"
                     /note="lac repressor"
     RBS             1208..1216
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1428..2177
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     terminator      2291..2377
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    2502..2523
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2538..2568
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2576..2592
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2600..2616
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     RBS             2681..2692
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             2699..3412
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     rep_origin      complement(3571..4159)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(4247..4341)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(4365..5021)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(5022..5124)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc
       61 ttcacctcga gtggcagttc cctactctcg catggggaga ccccacacta ccatcggcgc
      121 ttcattggcc agactctaag cggctcactt ggcgcgctaa ttgcatcagg ctgtccgcca
      181 gggcacgcgg gctcgccgtc tgggtgttcg gggctaacgt cgtcttgcgc ttaactaagc
      241 tcaccggcag taattggtta cctttaacgg cttgaccttg gctcagctgt aacagacgat
      301 caacagacgt ctggcctaat aaacggaagt cttgcttaat cgtcgtgctt ggtggaatgt
      361 agcagctaga atcctccgtg tcatcatagc caaccacgct aatgtcggcg cccacacgta
      421 agccgctttc cgtgatcgca cgcatcgcac ctaaggccat ttggtcattc gccactaaca
      481 tggcggtcgg cacaatacct tcgtttaaca tctgcatcgt ctgctggaag ccgctcatcg
      541 cgctccaatc accctcgcgc tcggcaattg gttggatctg gttacgggtc aggtacttgt
      601 gccaacccgc taagcgtaag cgggcgctca cgctgctcag cggaccggcc agtaaggcaa
      661 tctgttgatg gcccagcgcc actaagtgtt caacacctaa gcgggtgcca tcctcgtggc
      721 taaagatgat gctattaatc ggggtttgat cgctcacgtc caggaacagg gctggcacgt
      781 tggtacacgc cgcctcaacc gcgatcgcgt cttgatcgtc taatgggtaa ttgataatta
      841 agccgctcac acgctgggcc agcaggttat gaacggcggc cttgcacgcc tccacaccag
      901 agcgctccac catgctaaca acaacagacg cgcctaactg gtccgcacgg ctcttgatgg
      961 cggccacgat ctggcttggg gcatgtaacg ctaagctgct cgtcgccaca ccgattaaca
     1021 ggctttgctt accggctaac tgctgcgcaa cacgattcgg gatatagttt aattcggcca
     1081 tggcggcctc aaccttctcg cgggtcttgg cgctcacatg agacgcttga ttaacaacac
     1141 ggctcaccgt ttggtagctc acgcccgcgt attccgccac gtcatacagg gtcaccggct
     1201 tcatacaacc tccttagcca ctagttttat tcgactataa caaaccattt tcttgcgtaa
     1261 acctgtacga tcctacaggt gcttatgtta agtaattgta ttcccagcga tacaatagtg
     1321 tgacaaaaat ccaatttatt agaatcaaat gtcaatccat taccgtttta atgatatata
     1381 acacgcaaaa cttgcgacaa acaataggta aggataaaga gatgggtatg aaaaacataa
     1441 atgccgacga cacatacaga ataattaata aaattaaagc ttgtagaagc aataatgata
     1501 ttaatcaatg cttatctgat atgactaaaa tggtacattg tgaatattat ttactcgcga
     1561 tcatttatcc tcattctatg gttaaatctg atatttcaat tctagataat taccctaaaa
     1621 aatggaggca atattatgat gacgctaatt taataaaata tgatcctata gtagattatt
     1681 ctaactccaa tcattcacca attaattgga atatatttga aaacaatgct gtaaataaaa
     1741 aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct tatcactggg tttagtttcc
     1801 ctattcatac ggctaacaat ggcttcggaa tgcttagttt tgcacattca gaaaaagaca
     1861 actatataga tagtttattt ttacatgcgt gtatgaacat accattaatt gttccttctc
     1921 tagttgataa ttatcgaaaa ataaatatag caaataataa atcaaacaac gatttaacca
     1981 aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa aagctcttgg gatatttcaa
     2041 aaatattagg ctgcagtgag cgtactgtca ctttccattt aaccaatgcg caaatgaaac
     2101 tcaatacaac aaaccgctgc caaagtattt ctaaagcaat tttaacagga gcaattgatt
     2161 gcccatactt taaaaattaa gtacgtaagt aaagctttat tccggaggta cccgacgcgt
     2221 acaagcttgg atccctgcag gcctcagggc ccgatcgatg cggccgctta attaattaat
     2281 ctagaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
     2341 ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgccctaga cctaggcgtt
     2401 cggctgcggc gagcggtatc agctcactca aagcataatc acctatgaag ctagcacgac
     2461 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     2521 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     2581 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgcatgc
     2641 ctgcaggtcg actctagagg atccccgggt acctagaatt aaagaggaga aattaagcat
     2701 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
     2761 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
     2821 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt
     2881 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
     2941 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa
     3001 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
     3061 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
     3121 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
     3181 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
     3241 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct
     3301 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct
     3361 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aaaggcctgc
     3421 agcaaacgac gaaaactacg ctttagtagc ttaataagct taattagctg acctactagt
     3481 cggccgtctc gacatgaacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3541 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3601 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3661 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3721 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3781 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3841 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3901 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3961 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     4021 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     4081 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     4141 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     4201 gaacgaaaac tcacgttaag ggattttggt catgactagt gcttggattc tcaccaataa
     4261 aaaacgcccg gcggcaaccg agcgttctga acaaatccag atggagttct gaggtcatta
     4321 ctggatctat caacaggagt ccaagcgagc tcgatatcaa attacgcccc gccctgccac
     4381 tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc atcacagacg
     4441 gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt ataatatttg
     4501 cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa atcaaaactg
     4561 gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa ccctttaggg
     4621 aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg tagaaactgc
     4681 cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg ctcatggaaa
     4741 acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt cattgccata
     4801 cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc cggataaaac
     4861 ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg aacggtctgg
     4921 ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg atgccattgg
     4981 gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct
     5041 gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag
     5101 ttggaacctc ttacgtgccg atcaacgtct cattttcgcc agatatcgac gtctaag
//
