LOCUS       V005479                 5090 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005479
VERSION     V005479
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5090)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     A synthetic multicellular system for programmed pattern formation
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5090)
  AUTHORS   Basu S, Gerchman Y, Collins CH, Arnold FH, Weiss R.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JAN-2005) Electrical Engineering, Princeton
            University, J-319, Engineering Quadrangle, Olden Street, Princeton,
            NJ 08544, USA
REFERENCE   3  (bases 1 to 5090)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5090)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-JAN-2005) Electrical Engineering, Princeton University, J-319,
            Engineering Quadrangle, Olden Street, Princeton, NJ 08544, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(64..1143)
                     /label="lacI"
                     /note="lac repressor"
     RBS             1147..1155
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1371..2120
                     /gene="luxR"
                     /label="Transcriptional activator protein LuxR"
                     /note="Transcriptional activator protein LuxR from
                     Aliivibrio fischeri. Accession#: P12746"
     terminator      2191..2277
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     protein_bind    2374..2395
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2410..2440
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2448..2464
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2472..2488
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     RBS             2553..2564
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             2571..3284
                     /label="yeGFP"
                     /note="yeast-enhanced green fluorescent protein"
     rep_origin      complement(3443..4031)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     terminator      complement(4119..4213)
                     /label="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     CDS             complement(4237..4893)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(4894..4996)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tcacctcgag tggcagttcc ctactctcgc atggggagac cccacactac catcggcgct
       61 tcattggcca gactctaagc ggctcacttg gcgcgctaat tgcatcaggc tgtccgccag
      121 ggcacgcggg ctcgccgtct gggtgttcgg ggctaacgtc gtcttgcgct taactaagct
      181 caccggcagt aattggttac ctttaacggc ttgaccttgg ctcagctgta acagacgatc
      241 aacagacgtc tggcctaata aacggaagtc ttgcttaatc gtcgtgcttg gtggaatgta
      301 gcagctagaa tcctccgtgt catcatagcc aaccacgcta atgtcggcgc ccacacgtaa
      361 gccgctttcc gtgatcgcac gcatcgcacc taaggccatt tggtcattcg ccactaacat
      421 ggcggtcggc acaatacctt cgtttaacat ctgcatcgtc tgctggaagc cgctcatcgc
      481 gctccaatca ccctcgcgct cggcaattgg ttggatctgg ttacgggtca ggtacttgtg
      541 ccaacccgct aagcgtaagc gggcgctcac gctgctcagc ggaccggcca gtaaggcaat
      601 ctgttgatgg cccagcgcca ctaagtgttc aacacctaag cgggtgccat cctcgtggct
      661 aaagatgatg ctattaatcg gggtttgatc gctcacgtcc aggaacaggg ctggcacgtt
      721 ggtacacgcc gcctcaaccg cgatcgcgtc ttgatcgtct aatgggtaat tgataattaa
      781 gccgctcaca cgctgggcca gcaggttatg aacggcggcc ttgcacgcct ccacaccaga
      841 gcgctccacc atgctaacaa caacagacgc gcctaactgg tccgcacggc tcttgatggc
      901 ggccacgatc tggcttgggg catgtaacgc taagctgctc gtcgccacac cgattaacag
      961 gctttgctta ccggctaact gctgcgcaac acgattcggg atatagttta attcggccat
     1021 ggcggcctca accttctcgc gggtcttggc gctcacatga gacgcttgat taacaacacg
     1081 gctcaccgtt tggtagctca cgcccgcgta ttccgccacg tcatacaggg tcaccggctt
     1141 catacaacct ccttagccac tagttttatt cgactataac aaaccatttt cttgcgtaaa
     1201 cctgtacgat cctacaggtg cttatgttaa gtaattgtat tcccagcgat acaatagtgt
     1261 gacaaaaatc caatttatta gaatcaaatg tcaatccatt accgttttaa tgatatataa
     1321 cacgcaaaac ttgcgacaaa caataggtaa ggataaagag atgggtgcat atgaaaaaca
     1381 taaatgccga cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg
     1441 atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg
     1501 cgatcattta tcctcattct atggttaaat ctgatatttc aattctagat aattacccta
     1561 aaaaatggag gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt
     1621 attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata
     1681 aaaaatctcc aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt
     1741 tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag
     1801 acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt
     1861 ctctagttga taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa
     1921 ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt
     1981 caaaaatatt aggctgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga
     2041 aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg
     2101 attgcccata ctttaaaaat taagtacggg atccctgcag gcctcagggc ccgatcgatg
     2161 cggccgctta attaattaat ctagaggcat caaataaaac gaaaggctca gtcgaaagac
     2221 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
     2281 ccgccctaga cctaggggat atatgatgaa tcacctatga agctagcacg acaggtttcc
     2341 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     2401 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2461 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttgcat gcctgcaggt
     2521 cgactctaga ggatccccgg gtacctagaa ttaaagagga gaaattaagc atgcgtaaag
     2581 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     2641 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     2701 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
     2761 tcggttatgg tgttcaatgc tttgcgagat acccagatca tatgaaacag catgactttt
     2821 tcaagagtgc catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg
     2881 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     2941 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     3001 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
     3061 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
     3121 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     3181 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     3241 taacagctgc tgggattaca catggcatgg atgaactata caaaaggcct gcagcaaacg
     3301 acgaaaacta cgctttagta gcttaataag cttaattagc tgacctacta gtcggccgtc
     3361 tcgacatgaa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     3421 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     3481 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     3541 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     3601 ccgcctttct cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca
     3661 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     3721 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     3781 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     3841 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     3901 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     3961 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     4021 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     4081 actcacgtta agggattttg gtcatgacta gtgcttggat tctcaccaat aaaaaacgcc
     4141 cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct
     4201 atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc actcatcgca
     4261 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg
     4321 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt
     4381 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact
     4441 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc
     4501 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc
     4561 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta
     4621 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc
     4681 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt
     4741 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt
     4801 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc
     4861 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
     4921 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc
     4981 tcttacgtgc cgatcaacgt ctcattttcg ccagatatcg acgtctaaga aaccattatt
     5041 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
//
