LOCUS       Exported                8055 bp DNA     circular SYN 13-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pHT43
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8055)
  AUTHORS   11111111
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          2932..2957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3021..3284
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          3982..5016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          5392..5898
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(111..761)
                     /codon_start=1
                     /label=Cm
                     /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE
                     GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV
                     KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL
                     LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL"
     primer_bind     1236..1252
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1295..2377)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     protein_bind    2761..2785
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     sig_peptide     2807..2899
                     /codon_start=1
                     /product="ÔöÇ¿µÄÐÅºÅëÄ£¬°±»ùËáT±äA£¬ºËËáÐòÁÐ±äÎªÏÂÃæ£º
                     GCA TCA GCT"
                     /label=amyQ signal peptide (SPamyQ)
                     /translation="MIQKRKRTVSFRLVLMCTLLFVSLPITKTSA"
     terminator      2932..2957
                     /label=trp terminator
     CDS             3021..3284
                     /codon_start=1
                     /label=ParA family protein
                     /translation="MVETYEANIGLLGIVPVLIKSGGSVDQYILDEARKEFGDDLLNST
                     IKLRERIKRFDVQGITEEDTHDKEALKLFNNLTMELIERVEG"
     CDS             3982..5016
                     /codon_start=1
                     /label=helix-turn-helix domain-containing protein
                     /translation="MSEVNLKGNTDELVYYRQQTTGNKIARKRIKKGKEEVYYVAETEE
                     KIWTEEQIKNFSLDKFGTHIPYIEGHYTILNNYFFDFWGYFLGAEGIALYAHLTRYAYG
                     SKDFCFPSLQTIAKKMDKTPVTVRGYLKLLERYGFIWKVNVRNKTKDNTEESPIFKIRR
                     KVPLLSEELLNGNPNIEIPDDEEAHVKKALKKEKEGLPKVLKKEHDEFVKKMMDESETI
                     NIPEALQYDTMYEDILSKGEIRKEIKKQIPNPTTSFESISMTTEEEKVDSTLKSEMQNR
                     VSKPSFDTWFKNTKIKIENKNCLLLVPSEFAFEWIKKRYLETIKTVLEEAGYVFEKIEL
                     RKVQ"
     CDS             complement(5392..5898)
                     /codon_start=1
                     /label=ORF-2
                     /translation="MPYSPTLLEGAVSDILLHKKILSPKDLRPEFLSKRFGIEIMTGKT
                     SYAYIDSDVKVFVLDKKVEEKERNYQFHKLFAHTLLHEENHLEIPMEVYEKHSEEAERL
                     TWVEAIPYHMLRYIDFSDPEFIKQASDVFYIPEKVVQNRINFMIQQQFEIDEFDNVNEK
                     VKSFA"
     promoter        6132..6236
                     /gene="bla"
                     /label=AmpR promoter
     CDS             6237..7097
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7268..7856
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa
       61 tgtatcgtta gaaaaccgac tgtaaaaagt acagtcggca ttatctcata ttataaaagc
      121 cagtcattag gcctatctga caattcctga atagagttca taaacaatcc tgcatgataa
      181 ccatcacaaa cagaatgatg tacctgtaaa gatagcggta aatatattga attaccttta
      241 ttaatgaatt ttcctgctgt aataatgggt agaaggtaat tactattatt attgatattt
      301 aagttaaacc cagtaaatga agtccatgga ataatagaaa gagaaaaagc attttcaggt
      361 ataggtgttt tgggaaacaa tttccccgaa ccattatatt tctctacatc agaaaggtat
      421 aaatcataaa actctttgaa gtcattcttt acaggagtcc aaataccaga gaatgtttta
      481 gatacaccat caaaaattgt ataaagtggc tctaacttat cccaataacc taactctccg
      541 tcgctattgt aaccagttct aaaagctgta tttgagttta tcacccttgt cactaagaaa
      601 ataaatgcag ggtaaaattt atatccttct tgttttatgt ttcggtataa aacactaata
      661 tcaatttctg tggttatact aaaagtcgtt tgttggttca aataatgatt aaatatctct
      721 tttctcttcc aattgtctaa atcaatttta ttaaagttca tttgatatgc ctcctaaatt
      781 tttatctaaa gtgaatttag gaggcttact tgtctgcttt cttcattaga atcaatcctt
      841 ttttaaaagt caatattact gtaacataaa tatatatttt aaaaatatcc cactttatcc
      901 aattttcgtt tgttgaacta atgggtgctt tagttgaaga ataaaagacc acattaaaaa
      961 atgtggtctt ttgtgttttt ttaaaggatt tgagcgtagc gaaaaatcct tttctttctt
     1021 atcttgataa taagggtaac tattgccgtc gtccattccg acagcatcgc cagtcactat
     1081 ggcgtgctgc tagcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     1141 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     1201 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcga
     1261 gctcaggcct taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1321 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1381 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     1441 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     1501 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     1561 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     1621 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     1681 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     1741 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     1801 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     1861 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     1921 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     1981 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     2041 tgtgcaccgc cgttttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     2101 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     2161 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     2221 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     2281 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     2341 catactctgc gacatcgtat aacgttactg gtttcatcaa aatcgtctcc ctccgtttga
     2401 atatttgatt gatcgtaacc agatgaagca ctctttccac tatccctaca gtgttatggc
     2461 ttgaacaatc acgaaacaat aattggtacg tacgatcttt cagccgactc aaacatcaaa
     2521 tcttacaaat gtagtctttg aaagtattac atatgtaaga tttaaatgca accgtttttt
     2581 cggaaggaaa tgatgacctc gtttccaccg gaattagctt ggtaccagct attgtaacat
     2641 aatcggtacg ggggtgaaaa agctaacgga aaagggagcg gaaaagaatg atgtaagcgt
     2701 gaaaaatttt ttatcttatc acttgaaatt ggaagggaga ttctttatta taagaattgt
     2761 ggaattgtga gcggataaca attcccaatt aaaggaggaa ggatcaatga ttcaaaaacg
     2821 aaagcggaca gtttcgttca gacttgtgct tatgtgcacg ctgttatttg tcagtttgcc
     2881 gattacaaaa acatcagccg taggatcctc tagagtcgac gtccccgggg cagcccgcct
     2941 aatgagcggg cttttttcac gtcacgcgtc catggagatc tttgtctgca actgaaaagt
     3001 ttatacctta cctggaacaa atggttgaaa catacgaggc taatatcggc ttattaggaa
     3061 tagtccctgt actaataaaa tcaggtggat cagttgatca gtatattttg gacgaagctc
     3121 ggaaagaatt tggagatgac ttgcttaatt ccacaattaa attaagggaa agaataaagc
     3181 gatttgatgt tcaaggaatc acggaagaag atactcatga taaagaagct ctaaaactat
     3241 tcaataacct tacaatggaa ttgatcgaaa gggtggaagg ttaatggtac gaaaattagg
     3301 ggatctacct agaaagccac aaggcgatag gtcaagctta aagaaccctt acatggatct
     3361 tacagattct gaaagtaaag aaacaacaga ggttaaacaa acagaaccaa aaagaaaaaa
     3421 agcattgttg aaaacaatga aagttgatgt ttcaatccat aataagatta aatcgctgca
     3481 cgaaattctg gcagcatccg aagggaattc atattactta gaggatacta ttgagagagc
     3541 tattgataag atggttgaga cattacctga gagccaaaaa actttttatg aatatgaatt
     3601 aaaaaaaaga accaacaaag gctgagacag actccaaacg agtctgtttt tttaaaaaaa
     3661 atattaggag cattgaatat atattagaga attaagaaag acatgggaat aaaaatattt
     3721 taaatccagt aaaaatatga taagattatt tcagaatatg aagaactctg tttgtttttg
     3781 atgaaaaaac aaacaaaaaa aatccaccta acggaatctc aatttaacta acagcggcca
     3841 aactgagaag ttaaatttga gaaggggaaa aggcggattt atacttgtat ttaactatct
     3901 ccattttaac attttattaa accccataca agtgaaaatc ctcttttaca ctgttccttt
     3961 aggtgatcgc ggagggacat tatgagtgaa gtaaacctaa aaggaaatac agatgaatta
     4021 gtgtattatc gacagcaaac cactggaaat aaaatcgcca ggaagagaat caaaaaaggg
     4081 aaagaagaag tttattatgt tgctgaaacg gaagagaaga tatggacaga agagcaaata
     4141 aaaaactttt ctttagacaa atttggtacg catatacctt acatagaagg tcattataca
     4201 atcttaaata attacttctt tgatttttgg ggctattttt taggtgctga aggaattgcg
     4261 ctctatgctc acctaactcg ttatgcatac ggcagcaaag acttttgctt tcctagtcta
     4321 caaacaatcg ctaaaaaaat ggacaagact cctgttacag ttagaggcta cttgaaactg
     4381 cttgaaaggt acggttttat ttggaaggta aacgtccgta ataaaaccaa ggataacaca
     4441 gaggaatccc cgatttttaa gattagacgt aaggttcctt tgctttcaga agaactttta
     4501 aatggaaacc ctaatattga aattccagat gacgaggaag cacatgtaaa gaaggcttta
     4561 aaaaaggaaa aagagggtct tccaaaggtt ttgaaaaaag agcacgatga atttgttaaa
     4621 aaaatgatgg atgagtcaga aacaattaat attccagagg ccttacaata tgacacaatg
     4681 tatgaagata tactcagtaa aggagaaatt cgaaaagaaa tcaaaaaaca aatacctaat
     4741 cctacaacat cttttgagag tatatcaatg acaactgaag aggaaaaagt cgacagtact
     4801 ttaaaaagcg aaatgcaaaa tcgtgtctct aagccttctt ttgatacctg gtttaaaaac
     4861 actaagatca aaattgaaaa taaaaattgt ttattacttg taccgagtga atttgcattt
     4921 gaatggatta agaaaagata tttagaaaca attaaaacag tccttgaaga agctggatat
     4981 gttttcgaaa aaatcgaact aagaaaagtg caataaactg ctgaagtatt tcagcagttt
     5041 tttttattta gaaatagtga aaaaaatata atcagggagg tatcaatatt taatgagtac
     5101 tgatttaaat ttatttagac tggaattaat aattaacacg tagactaatt aaaatttaat
     5161 gagggataaa gaggatacaa aaatattaat ttcaatccct attaaatttt aacaaggggg
     5221 ggattaaaat ttaattagag gtttatccac aagaaaagac cctaataaaa tttttactag
     5281 ggttataaca ctgattaatt tcttaatggg ggagggatta aaatttaatg acaaagaaaa
     5341 caatctttta agaaaagctt ttaaaagata ataataaaaa gagctttgcg attaagcaaa
     5401 actctttact ttttcattga cattatcaaa ttcatcgatt tcaaattgtt gttgtatcat
     5461 aaagttaatt ctgttttgca caaccttttc aggaatataa aacacatctg aggcttgttt
     5521 tataaactca gggtcgctaa agtcaatgta acgtagcata tgatatggta tagcttccac
     5581 ccaagttagc ctttctgctt cttctgaatg tttttcatat acttccatgg gtatctctaa
     5641 atgattttcc tcatgtagca aggtatgagc aaaaagttta tggaattgat agttcctctc
     5701 tttttcttca acttttttat ctaaaacaaa cactttaaca tctgagtcaa tgtaagcata
     5761 agatgttttt ccagtcataa tttcaatccc aaatctttta gacagaaatt ctggacgtaa
     5821 atcttttggt gaaagaattt ttttatgtag caatatatcc gatacagcac cttctaaaag
     5881 cgttggtgaa tagggcattt tacctatctc ctctcatttt gtggaataaa aatagtcata
     5941 ttcgtccatc tacctatcct attatcgaac agttgaactt tttaatcaag gatcagtcct
     6001 ttttttcatt attcttaaac tgtgctctta actttaacaa ctcgatttgt ttttccagat
     6061 ctcgagggta actagcctcg ccgatcccgc aagaggcccg gcagtcaggt ggcacttttc
     6121 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6181 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6241 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6301 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     6361 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     6421 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     6481 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     6541 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     6601 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     6661 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     6721 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     6781 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     6841 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6901 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6961 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     7021 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     7081 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     7141 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     7201 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     7261 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     7321 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     7381 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     7441 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     7501 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     7561 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     7621 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     7681 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     7741 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     7801 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     7861 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     7921 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     7981 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     8041 gcccaatacg catgc
//
