LOCUS       V013558                 9090 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013558
VERSION     V013558
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9090)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(116..763)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     primer_bind     1238..1254
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1300..2379)
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    2763..2787
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2915..2932
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             3230..3943
                     /label="GFP (S65T)"
                     /note="S65T variant of Aequorea victoria green fluorescent
                     protein (Heim et al., 1995)"
     promoter        7167..7271
                     /label="AmpR promoter"
     CDS             7272..8129
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8303..8891
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa
       61 tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca tattataaaa
      121 gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat cctgcatgat
      181 aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt gaattacctt
      241 tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta ttattgatat
      301 ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa gcattttcag
      361 gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca tcagaaaggt
      421 ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca gagaatgttt
      481 tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa cctaactctc
      541 cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt gtcactaaga
      601 aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat aaaacactaa
      661 tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga ttaaatatct
      721 cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat gcctcctaaa
      781 tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta gaatcaatcc
      841 ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat
      901 ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac cacattaaaa
      961 aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc ttttctttct
     1021 tatcttgata ataagggtaa ctattgccga tcgtccattc cgacagcatc gccagtcact
     1081 atggcgtgct gctagcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
     1141 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     1201 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc
     1261 gagctcaggc cttaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     1321 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     1381 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
     1441 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
     1501 aaaatcctgt ttgatggtgg ttgacggcgg gatataacat gagctgtctt cggtatcgtc
     1561 gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
     1621 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
     1681 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
     1741 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
     1801 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
     1861 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
     1921 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
     1981 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
     2041 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
     2101 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
     2161 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
     2221 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
     2281 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
     2341 ggcatactct gcgacatcgt ataacgttac tggtttcatc aaaatcgtct ccctccgttt
     2401 gaatatttga ttgatcgtaa ccagatgaag cactctttcc actatcccta cagtgttatg
     2461 gcttgaacaa tcacgaaaca ataattggta cgtacgatct ttcagccgac tcaaacatca
     2521 aatcttacaa atgtagtctt tgaaagtatt acatatgtaa gatttaaatg caaccgtttt
     2581 ttcggaagga aatgatgacc tcgtttccac cggaattagc ttggtaccag ctattgtaac
     2641 ataatcggta cgggggtgaa aaagctaacg gaaaagggag cggaaaagaa tgatgtaagc
     2701 gtgaaaaatt ttttatctta tcacttgaaa ttggaaggga gattctttat tataagaatt
     2761 gtggaattgt gagcggataa caattcccaa ttaaaggagg aaggatcaat gattcaaaaa
     2821 cgaaagcgga cagtttcgtt cagacttgtg cttatgtgca cgctgttatt tgtcagtttg
     2881 ccgattacaa aaacatcagc cgtaggatcc gatgcatcat catcatcatc atggctctct
     2941 tcaagattct gaagttaacc aagaagctaa acctgaagtt aaacctgaag ttaaacctga
     3001 aacacatatc aaccttaaag tttctgatgg ctcttctgaa atcttcttca aaatcaaaaa
     3061 aacaacacct cttcgtcgtc ttatggaagc tttcgctaaa cgtcaaggca aagaaatgga
     3121 ttctcttcgt ttcctttacg atggcatccg tatccaagct gatcaagctc ctgaagatct
     3181 tgatatggaa gataacgatc atgaagctca tcgtgaacaa atcggcggca tgtctaaagg
     3241 cgaagaactt ttcacaggcg ttgttcctat ccttgttgaa cttgatggcg atgttaacgg
     3301 ccataaattc tctgtttctg gcgaaggcga aggcgatgct acatacggca aacttacact
     3361 taaattcatc tgcacaacag gcaaacttcc tgttccttgg cctacacttg ttacaacatt
     3421 cacatacggc gttcaatgct tctctcgtta ccctgatcat atgaaacaac atgatttctt
     3481 caaatctgct atgcctgaag gctacgttca agaacgtaca atcttcttca aagatgatgg
     3541 caactacaaa acacgtgctg aagttaaatt cgaaggcgat acacttgtta accgtatcga
     3601 acttaaaggc atcgatttca aagaagatgg caacatcctt ggccataaac ttgaatacaa
     3661 ctacaactct cataacgttt acatcatggc tgataaacaa aaaaacggca tcaaagttaa
     3721 cttcaaaatc cgtcataaca tcgaagatgg ctctgttcaa cttgctgatc attaccaaca
     3781 aaacacacct atcggcgatg gccctgttct tcttcctgat aaccattacc tttctacaca
     3841 atctgctctt tctaaagatc ctaacgaaaa acgtgatcat atggttcttc ttgaattcgt
     3901 tacagctgct ggcatcacac atggcatgga tgaactttac aaatctagag tcgacgtccc
     3961 cggggcagcc cgcctaatga gcgggctttt ttcacgtcac gcgtccatgg agatctttgt
     4021 ctgcaactga aaagtttata ccttacctgg aacaaatggt tgaaacatac gaggctaata
     4081 tcggcttatt aggaatagtc cctgtactaa taaaatcagg tggatcagtt gatcagtata
     4141 ttttggacga agctcggaaa gaatttggag atgacttgct taattccaca attaaattaa
     4201 gggaaagaat aaagcgattt gatgttcaag gaatcacgga agaagatact catgataaag
     4261 aagctctaaa actattcaat aaccttacaa tggaattgat cgaaagggtg gaaggttaat
     4321 ggtacgaaaa ttaggggatc tacctagaaa gccacaaggc gataggtcaa gcttaaagaa
     4381 cccttacatg gatcttacag attctgaaag taaagaaaca acagaggtta aacaaacaga
     4441 accaaaaaga aaaaaagcat tgttgaaaac aatgaaagtt gatgtttcaa tccataataa
     4501 gattaaatcg ctgcacgaaa ttctggcagc atccgaaggg aattcatatt acttagagga
     4561 tactattgag agagctattg ataagatggt tgagacatta cctgagagcc aaaaaacttt
     4621 ttatgaatat gaattaaaaa aaagaaccaa caaaggctga gacagactcc aaacgagtct
     4681 gtttttttaa aaaaaatatt aggagcattg aatatatatt agagaattaa gaaagacatg
     4741 ggaataaaaa tattttaaat ccagtaaaaa tatgataaga ttatttcaga atatgaagaa
     4801 ctctgtttgt ttttgatgaa aaaacaaaca aaaaaaatcc acctaacgga atctcaattt
     4861 aactaacagc ggccaaactg agaagttaaa tttgagaagg ggaaaaggcg gatttatact
     4921 tgtatttaac tatctccatt ttaacatttt attaaacccc atacaagtga aaatcctctt
     4981 ttacactgtt cctttaggtg atcgcggagg gacattatga gtgaagtaaa cctaaaagga
     5041 aatacagatg aattagtgta ttatcgacag caaaccactg gaaataaaat cgccaggaag
     5101 agaatcaaaa aagggaaaga agaagtttat tatgttgctg aaacggaaga gaagatatgg
     5161 acagaagagc aaataaaaaa cttttcttta gacaaatttg gtacgcatat accttacata
     5221 gaaggtcatt atacaatctt aaataattac ttctttgatt tttggggcta ttttttaggt
     5281 gctgaaggaa ttgcgctcta tgctcaccta actcgttatg catacggcag caaagacttt
     5341 tgctttccta gtctacaaac aatcgctaaa aaaatggaca agactcctgt tacagttaga
     5401 ggctacttga aactgcttga aaggtacggt tttatttgga aggtaaacgt ccgtaataaa
     5461 accaaggata acacagagga atccccgatt tttaagatta gacgtaaggt tcctttgctt
     5521 tcagaagaac ttttaaatgg aaaccctaat attgaaattc cagatgacga ggaagcacat
     5581 gtaaagaagg ctttaaaaaa ggaaaaagag ggtcttccaa aggttttgaa aaaagagcac
     5641 gatgaatttg ttaaaaaaat gatggatgag tcagaaacaa ttaatattcc agaggcctta
     5701 caatatgaca caatgtatga agatatactc agtaaaggag aaattcgaaa agaaatcaaa
     5761 aaacaaatac ctaatcctac aacatctttt gagagtatat caatgacaac tgaagaggaa
     5821 aaagtcgaca gtactttaaa aagcgaaatg caaaatcgtg tctctaagcc ttcttttgat
     5881 acctggttta aaaacactaa gatcaaaatt gaaaataaaa attgtttatt acttgtaccg
     5941 agtgaatttg catttgaatg gattaagaaa agatatttag aaacaattaa aacagtcctt
     6001 gaagaagctg gatatgtttt cgaaaaaatc gaactaagaa aagtgcaata aactgctgaa
     6061 gtatttcagc agtttttttt atttagaaat agtgaaaaaa atataatcag ggaggtatca
     6121 atatttaatg agtactgatt taaatttatt tagactggaa ttaataatta acacgtagac
     6181 taattaaaat ttaatgaggg ataaagagga tacaaaaata ttaatttcaa tccctattaa
     6241 attttaacaa gggggggatt aaaatttaat tagaggttta tccacaagaa aagaccctaa
     6301 taaaattttt actagggtta taacactgat taatttctta atgggggagg gattaaaatt
     6361 taatgacaaa gaaaacaatc ttttaagaaa agcttttaaa agataataat aaaaagagct
     6421 ttgcgattaa gcaaaactct ttactttttc attgacatta tcaaattcat cgatttcaaa
     6481 ttgttgttgt atcataaagt taattctgtt ttgcacaacc ttttcaggaa tataaaacac
     6541 atctgaggct tgttttataa actcagggtc gctaaagtca atgtaacgta gcatatgata
     6601 tggtatagct tccacccaag ttagcctttc tgcttcttct gaatgttttt catatacttc
     6661 catgggtatc tctaaatgat tttcctcatg tagcaaggta tgagcaaaaa gtttatggaa
     6721 ttgatagttc ctctcttttt cttcaacttt tttatctaaa acaaacactt taacatctga
     6781 gtcaatgtaa gcataagatg tttttccagt cataatttca atcccaaatc ttttagacag
     6841 aaattctgga cgtaaatctt ttggtgaaag aattttttta tgtagcaata tatccgatac
     6901 agcaccttct aaaagcgttg gtgaataggg cattttacct atctcctctc attttgtgga
     6961 ataaaaatag tcatattcgt ccatctacct atcctattat cgaacagttg aactttttaa
     7021 tcaaggatca gtcctttttt tcattattct taaactgtgc tcttaacttt aacaactcga
     7081 tttgtttttc cagatctcga gggtaactag cctcgccgat cccgcaagag gcccggcagt
     7141 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7201 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7261 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7321 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     7381 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     7441 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     7501 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     7561 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     7621 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7681 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7741 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7801 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7861 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7921 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7981 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     8041 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     8101 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     8161 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     8221 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     8281 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     8341 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     8401 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     8461 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     8521 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     8581 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     8641 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8701 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8761 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8821 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8881 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8941 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     9001 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     9061 aggaagcgga agagcgccca atacgcatgc
//
