LOCUS       V005482                 8299 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005482
VERSION     V005482
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8299)
  AUTHORS   Li Q, Yuan XE, Yin YP, Wang ZK.
  TITLE     Rapid determination of exogenous plasmid copy number and stability
            using relative quantitative real-time PCR in recombinant
            Brevibacillus brevis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8299)
  AUTHORS   Li Q, Yuan XE, Yin YP, Wang ZK.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2009) Bioengineering College of Chongqing
            University, Genetic Engineering Research Center, Shazheng Street,
            Chongqing, Chongqing 400030, China
REFERENCE   3  (bases 1 to 8299)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8299)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (29-SEP-2009) Bioengineering College of Chongqing University,
            Genetic Engineering Research Center, Shazheng Street, Chongqing,
            Chongqing 400030, China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     562..578
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1678..2391
                     /label="GFP"
                     /note="Aequorea victoria green fluorescent protein"
     primer_bind     complement(2419..2435)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2443..2459)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2467..2497)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2512..2533)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2821..3409)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3583..4440)
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(4595..5329)
                     /gene="erm"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Streptococcus pneumoniae. Accession#: P21236"
ORIGIN
        1 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
       61 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
      121 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
      181 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      241 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
      301 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
      361 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
      421 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
      481 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      541 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attcgagctc ggtacccggg
      601 gatccgaaat tagttataca agcattttgt aagaattatt aaaaagataa atcctgctat
      661 tacgagatta gtaggatgat attgtgaaaa attttttatc tattcgattt aaaatattta
      721 tgaattttac ataaacctca taagaaaaaa tactatctat actattttaa gaaatttatt
      781 agaataagcg gattcaaaat agccctggcc ataaaagtac ctcagcagta gaagttttga
      841 ccaaaattaa aaaaataccc aatcaagaga atattcttaa ttacaatacg ttttgcgagg
      901 aacatattga ttgaaattta ataaatttag tcctaaaatt taaagaaatt taagtttttc
      961 atatttttat gaactaacaa gaataaaaat tgtgtttatt tattattctt gttaaatatt
     1021 tgataaagag atatattttt ggtcgaaacg taagatgaaa ccttagataa aagtgctttt
     1081 tttgttgcaa ttgaagaatt attaatgtta agcttaatta aagataatat ctttgaattg
     1141 taacgcccct caaaagtaag aactacaaaa aaagaatacg ttatatagaa atatgtttga
     1201 accttcttca gattacaaat atattcggac ggactctacc tcaaatgctt atctaactat
     1261 agaatgacat acaagcacaa ccttgaaaat ttgaaaatat aactaccaat gaacttgttc
     1321 atgtgaatta tcgctgtatt taattttctc aattcaatat ataatatgcc aatacattgt
     1381 tacaagtaga aattaagaca cccttgatag ccttactata cctaacatga tgtagtatta
     1441 aatgaatatg taaatatatt tatgataaga agcgacttat ttataatcat tacatatttt
     1501 tctattggaa tgattaagat tccaatagaa tagtgtataa attatttatc ttgaaaggag
     1561 ggatgcctaa aaacgaagaa cattaaaaac atatatttgc accgtctaat ggatttatga
     1621 aaaatcattt tatcagtttg aaaattatgt attatgataa gaaagggagg aagaaaaatg
     1681 agtaaaggag aagaactttt cactggagtt gttccaattc ttgttgaatt agatggtgat
     1741 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
     1801 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
     1861 actactttcg cctatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat
     1921 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
     1981 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
     2041 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
     2101 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
     2161 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
     2221 tatcaacaaa atactccaat tggcgatggc cctgtccttc taccagacaa ccattacctg
     2281 tccacacaat ctgtcctttc gaaagacccc aacgaaaaga gagaccacat ggtccttctt
     2341 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataggcatgc
     2401 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     2461 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     2521 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     2581 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     2641 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     2701 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     2761 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     2821 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     2881 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     2941 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3001 cgtggcgctt tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     3061 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     3121 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     3181 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     3241 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     3301 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     3361 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     3421 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     3481 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     3541 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     3601 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     3661 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     3721 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     3781 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     3841 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     3901 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     3961 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4021 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     4081 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     4141 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     4201 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     4261 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4321 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4381 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4441 actcttcctt tttcaatatc gatacaaatt ccccgtaggc gctagggacc tctttagctt
     4501 cttggaagct gtcagtagta tatctaataa tttatctcca ttccctttag taacgtgtaa
     4561 ctttccaaat ttaaaaaagc gactcataga attatttcct cccgttaaat aatagataac
     4621 tattaaaaat agacaatact tgctcataag taatggtact taaattgttt actttggcgt
     4681 gtttcattgc ttgatgaaac tgatttttag taaacagttg acgatattct cgattgaccc
     4741 attttgaaac aaagtacgta tatagcttcc aatatttatc tggaacatct gtggtatggc
     4801 gggtaagttt tattaagaca ctgtttactt ttggtttagg atgaaagcat tccgctggca
     4861 gcttaagcaa ttgctgaatc gagacttgag tgtgcaagag caaccctagt gttcggtgaa
     4921 tatccaaggt acgcttgtag aatccttctt caacaatcag atagatgtca gacgcatggc
     4981 tttcaaaaac cactttttta ataatttgtg tgcttaaatg gtaaggaata ctcccaacaa
     5041 ttttatacct ctgtttgtta gggaattgaa actgtagaat atcttggtga attaaagtga
     5101 cacgaatgtt cagttttaat ttttctgacg ataagttgaa tagatgactg tctaattcaa
     5161 tagacgttac ctgtttactt attttagcca gtttcgtcgt taaatgccct ttacctgttc
     5221 caatttcgta aacggtatcg gtttctttta aattcaattg ttttattatt tggttgagta
     5281 ctttttcact cgttaaaaag ttttgagaat attttatatt tttgttcatg taattactcc
     5341 tgaagtgatt acatctgtaa ataaatacag aagttaaacg atttgtttgt aattttagtt
     5401 atctgtttaa aaagtcataa gattagtcac tggtaggaat taatctaacg tatttattta
     5461 tctgcgtaat cactgttttt agtctgtttc aaaacagtag atgttttatc tacattacgc
     5521 atttggaata ccaacatgac gaatccctcc ttcttaatta caaattttta gcatctaatt
     5581 taacttcaat tcctattata cacaaaattt taagatactg cactatcaac acactcttaa
     5641 gtttgcttct aaggatcaac atattagata tcgtggtgtt caaaaacgct acctcatgat
     5701 cctgcaatga tataacttta tcttgcaaat cctttacgac atcagtagat ggcgccgctg
     5761 taaatagtat ccccagcatc aatagttccc cctgattata taaattcaga cgaatactat
     5821 tatccattat ttttttatta aggtaaatta atattttacc tattaaagga gggacgattt
     5881 aagtatggac gtgatgtttg gtcaaggtgg gaacataaat tttaattaca ttggaacata
     5941 ttgtggacaa gcatctaatt cataatcccc actttccaag catgaaagtc ccttatacca
     6001 tcaattattc aataaaatat ccctatcata aacctgtccc gcgcgacggt tcaaatactc
     6061 ctttatgata aaataaagat ataaagagac gagatgaggg agaaatttga cggctaataa
     6121 aaaaaagatt gtaaataaac caactgttgt ggcaaaactt ccacaaatca ttatagatac
     6181 atataacttg acttgcactt ctaacgatgt aaaaatgttt cccggattta taaagcacgt
     6241 aaagaaacaa catcctggta tttatgaaaa atattcatcg cacataaaag atatcgttga
     6301 acaccccgac tatgtcgggc aaaatcctaa agaacctaat agcgttgaat tggtaaagat
     6361 tttaaatgac catatactaa ttgctataaa gttggaccca agcggttatt tgttcttatc
     6421 aactatgttc gatctaaaaa acggtcctgc gaaaatccaa agacgattga atagtggacg
     6481 tttaattgct tacaaagacc tgttaagcta acggggagtt tactaaaata taagaacatg
     6541 gtatactacc tgtagaaagt aacataataa tgatatagga tctttgaggt gggaaaggtt
     6601 ccccacgcgc ttatctaacc gccgttctca agcatttgat tggctaggta agttaccaga
     6661 gatgtaggat acgccgccct accaacgatc ctatgtacgg taagccatcc gttttttacg
     6721 gatggctttt tatattgact ttttcatcgc taatggttac ttttctatca aacgacaccg
     6781 aaaggcgatg attattgtgt taaaaaaccc aatattataa catccactaa tggataatct
     6841 tacttttagt aagtaggttg cctagtgaaa tacaataatt ccaacattgg gccttaatac
     6901 atattaatta gttttaaccc tctcactata aaagagttaa atatgactcc gcaacctctt
     6961 taatatcact attacatttt gtaacaacat tattactttc atcctctgta ccattaacaa
     7021 ataaatgagg attctttacc ataaagtcta acaatagatc aataaaccgc tgcctttgtt
     7081 ctctagccat ttcctttctc atagagaacc actcccctat ctttcactat tcttttaatt
     7141 tcatcttgtc tttcacttga tttaaacagc tctggattct tcctcaatcc cccactatct
     7201 tagttaatgg tagttgcttc agtactctac attttttgct aatcgaggtt aaaatccttc
     7261 aaatcccttg actttaaggt cttaaggttt tcatactggc gagtttgtta ggaaatctga
     7321 atgtagtttt gggcatagta acccctgttg aaaatttacg aggttaaaac gcccactcat
     7381 tcgtactggc ttagatgccg aggtctcgaa ttgagattgg ttgcattgac gacaaacggt
     7441 tatagcttac aaaactacta taccgtccgt atatagctta ttggcattgt attgcgccgt
     7501 acggtgcgtt tctcacgccc aacaccgttc cctttcgggt gattcgctat ataacccgca
     7561 tatccttgta ataaagcttg tacaagaata gccgtgaatc agtgtcatga tcgccgctta
     7621 cgagtaactg tttaactcca gtcgcacaat catgtcttat tttgaacccc caacactttt
     7681 tgccgctaac gtcttggtta tcatcattat taggatttac atcccgtagg cttgttttat
     7741 gcaaggcttg tgtcctttac ctcgcttacg tcagacagtt gaatgcaacg acaactgata
     7801 tccgagtgta ctctcctgca ttggctacac tagaccatct ttttaaagtg gtagcttcca
     7861 ctggaacgga acacaaaaag ggcgttctct atatctaaac gccctgtaca tttacaagac
     7921 ttctaggtat agaaaacgcc cttttatatc cgttttatta tgtatatgac aatgctagta
     7981 attactagtt gaaatattcg tagagtaacg gtacaatagg tgtatctaat aagccttgtt
     8041 cgcgaaaaca aggcaaataa cttatttgag gttggcgcct cttataagtc gaagtcctgt
     8101 atggctgtac aggattaagt cattctcgct aaaggttggt agccaatagc atatgagagt
     8161 ggcttttttc atttccgttc tattaagtta ctgctaattt taccgctatg tttctattaa
     8221 atcaacgaaa atttgataag gttcaccaga aacattaata tttgcgacat aaaactcact
     8281 ctccaacttt ggaggatgg
//
