LOCUS       V001727                 6530 bp    DNA     circular SYN 13-JAN-2022
DEFINITION  Exported.
ACCESSION   V001727
VERSION     V001727
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6530)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6530
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2973..3707
                     /gene="erm"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Streptococcus pneumoniae. Accession#: P21236"
     CDS             3862..4719
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      4893..5481
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    5769..5790
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5805..5835
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    5843..5859
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5867..5883
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(5896..5952)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(5953..5969)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 ccatcctcca aagttggaga gtgagtttta tgtcgcaaat attaatgttt ctggtgaacc
       61 ttatcaaatt ttcgttgatt taatagaaac atagcggtaa aattagcagt aacttaatag
      121 aacggaaatg aaaaaagcca ctctcatatg ctattggcta ccaaccttta gcgagaatga
      181 cttaatcctg tacagccata caggacttcg acttataaga ggcgccaacc tcaaataagt
      241 tatttgcctt gttttcgcga acaaggctta ttagatacac ctattgtacc gttactctac
      301 gaatatttca actagtaatt actagcattg tcatatacat aataaaacgg atataaaagg
      361 gcgttttcta tacctagaag tcttgtaaat gtacagggcg tttagatata gagaacgccc
      421 tttttgtgtt ccgttccagt ggaggctacc actttaaaaa gatggtctag tgtagccaat
      481 gcaggagagt acactcggat atcagttgtc gttgcattca actgtctgac gtaagcgagg
      541 taaaggacac aagccttgca taaaacaagc ctacgggatg taaatcctaa taatgatgat
      601 aaccaagacg ttagcggcaa aaagtgttgg gggttcaaaa taagacatga ttgtgcgact
      661 ggagttaaac agttactcgt aagcggcgat catgacactg attcacggct attcttgtac
      721 aaactttatt acaaggatat gcgggttata tagcgaatca cccgaaaggg aacggtgttg
      781 ggcgtgagaa acgcaccgta cggcgcaata caatgccaat aagctatata cggacggtat
      841 agtagttttg taagctataa ccgtttgtcg tcaatgcaac caatctcaat tcgagacctc
      901 ggcatctaag ccagtacgaa tgagtgggcg ttttaacctc gtaaattttc aacaggggtt
      961 actatgccca aaactacatt cagatttcct aacaaactcg ccagtatgaa aaccttaaga
     1021 ccttaaagtc aagggatttg aaggatttta acctcgatta gcaaaaaatg tagagtactg
     1081 aagcaactac cattaactaa gatagtgggg gattgaggaa gaatccagag ctgtttaaat
     1141 caagtgaaag acaagatgaa attaaaagaa tagtgaaaga taggggagtg gttctctatg
     1201 agaaaggaaa tggctagaga acaaaggcag cggtttattg atctattgtt agactttatg
     1261 gtaaagaatc ctcatttatt tgttaatggt acagaggatg aaagtaataa tgttgttaca
     1321 aaatgtaata gtgatattaa agaggttgcg gagtcatatt taactctttt atagtgagag
     1381 ggttaaaact aattaatatg tattaaggcc caatgttgga attattgtat ttcactaggc
     1441 aacctactta ctaaaagtaa gattatccat tagtggatgt tataatattg ggttttttaa
     1501 cacaataatc atcgcctttc ggtgtcgttt gatagaaaag taaccattag cgatgaaaaa
     1561 gtcaatataa aaagccatcc gtaaaaaacg gatggcttac cgtacatagg atcgttggta
     1621 gggcggcgta tcctacatct ctggtaactg acctagccaa tcaaatgctt gagaacggcg
     1681 gttagataag cgcgtgggga acctttccca cctcaaagat cctatatcat tattatgtta
     1741 ctttctacag gtagtatacc atgttcttat attttagtaa actccccgtt agcttaacag
     1801 gtctttgtaa gcaattaaac gtccactatt caatcgtctt tggattttcg caggaccgtt
     1861 ttttagatcg aacatagttg ataagaacaa ataaccgctt gggtccaact ttatagcaat
     1921 tagtatatgg tcatttaaaa tctttaccaa ttcaacgcta ttaggttctt taggattttg
     1981 cccgacatag tcggggtgtt caacgatatc ttttatgtgc gatgaatatt tttcataaat
     2041 accaggatgt tgtttcttta cgtgctttat aaatccggga aacattttta catcgttaga
     2101 agtgcaagtc aagttatatg tatctataat gatttgtgga agttttgcca caacagttgg
     2161 tttatttaca atcttttttt tattagccgt caaatttctc cctcatctcg tctctttata
     2221 tctttatttt atcataaagg agtatttgaa ccgtcgcgcg ggacaggttt atgataggga
     2281 tattttattg aataattgat ggtataaggg actttcatgc ttggaaagtg gggattatga
     2341 attagatgct tgtccacaat atgttccaat gtaattaaaa tttatgttcc caccttgacc
     2401 aaacatcacg tccatactta aatcgtccct cctttaatag gtaaaatatt aatttacctt
     2461 aataaaaaaa taatggataa tagtattcgt ctgaatttat ataatcaggg ggaactattg
     2521 atgctgggga tactatttac agcggcgcca tctactgatg tcgtaaagga tttgcaagat
     2581 aaagttatat cattgcagga tcatgaggta gcgtttttga acaccacgat atctaatatg
     2641 ttgatcccct tagaagcaaa cttaagagtg tgttgatagt gcagtatctt aaaattttgt
     2701 gtataatagg aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagggatt
     2761 cgtcatgttg gtattccaaa tgcgtaatgt agataaaaca tctactgttt tgaaacagac
     2821 taaaaacagt gattacgcag ataaataaat acgttagatt aattcctacc agtgactaat
     2881 cttatgactt tttaaacaga taactaaaat tacaaacaaa tcgtttaact tctgtattta
     2941 tttacagatg taatcacttc aggagtaatt acatgaacaa aaatataaaa tattctcaaa
     3001 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
     3061 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
     3121 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgacagaaa
     3181 aattaaaact gaacattcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
     3241 ctaacaaaca gaggtataaa attgttggga gtattcctta ccatttaagc acacaaatta
     3301 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
     3361 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
     3421 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca aaagtaaaca
     3481 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
     3541 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
     3601 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccatt acttatgagc
     3661 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
     3721 cgctttttta aatttggaaa gttacacgtt actaaaggga atggagataa attattagat
     3781 atactactga cagcttccaa gaaggtaaag aggtccctag cgcctacggg gaatttgtat
     3841 cgggattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3901 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3961 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4021 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4081 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4141 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4201 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4261 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4321 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4381 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4441 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4501 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4561 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4621 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4681 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4741 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4801 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4861 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4921 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4981 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     5041 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5101 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5161 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5221 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5281 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5341 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5401 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5461 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5521 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5581 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5641 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     5701 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     5761 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     5821 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     5881 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
     5941 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     6001 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     6061 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     6121 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     6181 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     6241 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     6301 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     6361 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     6421 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6481 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
//
