LOCUS       V013187                 7938 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013187
VERSION     V013187
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7938
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(116..763)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     primer_bind     1239..1255
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1301..2380)
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    2709..2733
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             2776..2799
                     /label="8xHis"
                     /note="8xHis affinity tag"
     promoter        6015..6119
                     /label="AmpR promoter"
     CDS             6120..6977
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7151..7739
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa
       61 tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca tattataaaa
      121 gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat cctgcatgat
      181 aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt gaattacctt
      241 tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta ttattgatat
      301 ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa gcattttcag
      361 gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca tcagaaaggt
      421 ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca gagaatgttt
      481 tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa cctaactctc
      541 cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt gtcactaaga
      601 aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat aaaacactaa
      661 tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga ttaaatatct
      721 cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat gcctcctaaa
      781 tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta gaatcaatcc
      841 ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat
      901 ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaaaga ccacattaaa
      961 aaatgtggtc ttttgtgttt ttttaaagga tttgagcgta gcgaaaaatc cttttctttc
     1021 ttatcttgat aataagggta actattgccg atcgtccatt ccgacagcat cgccagtcac
     1081 tatggcgtgc tgctagcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
     1141 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     1201 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     1261 cgagctcagg ccttaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     1321 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     1381 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
     1441 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
     1501 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
     1561 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
     1621 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
     1681 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
     1741 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
     1801 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
     1861 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
     1921 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
     1981 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
     2041 gattgtgcac cgccgtttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
     2101 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
     2161 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
     2221 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
     2281 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
     2341 cggcatactc tgcgacatcg tataacgtta ctggtttcat caaaatcgtc tccctccgtt
     2401 tgaatatttg attgatcgta accagatgaa gcactctttc cactatccct acagtgttat
     2461 ggcttgaaca atcacgaaac aataattggt acgtacgatc tttcagccga ctcaaacatc
     2521 aaatcttaca aatgtagtct ttgaaagtat tacatatgta agatttaaat gcaaccgttt
     2581 tttcggaagg aaatgatgac ctcgtttcca ccggaattag cttggtacca aaggaggtaa
     2641 ggatcactag aaaatttttt aaaaaatctc ttgacattgg aagggagata tgttattata
     2701 agaattgcgg aattgtgagc ggataacaat tcccatataa aggaggaagg atccatgtct
     2761 agagtcgacg tcgctcatca ccatcaccat caccatcact aacgtccccg gggcagcccg
     2821 cctaatgagc gggctttttt cacgtcacgc gtccatggag atctttgtct gcaactgaaa
     2881 agtttatacc ttacctggaa caaatggttg aaacatacga ggctaatatc ggcttattag
     2941 gaatagtccc tgtactaata aaatcaggtg gatcagttga tcagtatatt ttggacgaag
     3001 ctcggaaaga atttggagat gacttgctta attccacaat taaattaagg gaaagaataa
     3061 agcgatttga tgttcaagga atcacggaag aagatactca tgataaagaa gctctaaaac
     3121 tattcaataa ccttacaatg gaattgatcg aaagggtgga aggttaatgg tacgaaaatt
     3181 aggggatcta cctagaaagc cacaaggcga taggtcaagc ttaaagaacc cttacatgga
     3241 tcttacagat tctgaaagta aagaaacaac agaggttaaa caaacagaac caaaaagaaa
     3301 aaaagcattg ttgaaaacaa tgaaagttga tgtttcaatc cataataaga ttaaatcgct
     3361 gcacgaaatt ctggcagcat ccgaagggaa ttcatattac ttagaggata ctattgagag
     3421 agctattgat aagatggttg agacattacc tgagagccaa aaaacttttt atgaatatga
     3481 attaaaaaaa agaaccaaca aaggctgaga cagactccaa acgagtctgt ttttttaaaa
     3541 aaaatattag gagcattgaa tatatattag agaattaaga aagacatggg aataaaaata
     3601 ttttaaatcc agtaaaaata tgataagatt atttcagaat atgaagaact ctgtttgttt
     3661 ttgatgaaaa aacaaacaaa aaaaatccac ctaacggaat ctcaatttaa ctaacagcgg
     3721 ccaaactgag aagttaaatt tgagaagggg aaaaggcgga tttatacttg tatttaacta
     3781 tctccatttt aacattttat taaaccccat acaagtgaaa atcctctttt acactgttcc
     3841 tttaggtgat cgcggaggga cattatgagt gaagtaaacc taaaaggaaa tacagatgaa
     3901 ttagtgtatt atcgacagca aaccactgga aataaaatcg ccaggaagag aatcaaaaaa
     3961 gggaaagaag aagtttatta tgttgctgaa acggaagaga agatatggac agaagagcaa
     4021 ataaaaaact tttctttaga caaatttggt acgcatatac cttacataga aggtcattat
     4081 acaatcttaa ataattactt ctttgatttt tggggctatt ttttaggtgc tgaaggaatt
     4141 gcgctctatg ctcacctaac tcgttatgca tacggcagca aagacttttg ctttcctagt
     4201 ctacaaacaa tcgctaaaaa aatggacaag actcctgtta cagttagagg ctacttgaaa
     4261 ctgcttgaaa ggtacggttt tatttggaag gtaaacgtcc gtaataaaac caaggataac
     4321 acagaggaat ccccgatttt taagattaga cgtaaggttc ctttgctttc agaagaactt
     4381 ttaaatggaa accctaatat tgaaattcca gatgacgagg aagcacatgt aaagaaggct
     4441 ttaaaaaagg aaaaagaggg tcttccaaag gttttgaaaa aagagcacga tgaatttgtt
     4501 aaaaaaatga tggatgagtc agaaacaatt aatattccag aggccttaca atatgacaca
     4561 atgtatgaag atatactcag taaaggagaa attcgaaaag aaatcaaaaa acaaatacct
     4621 aatcctacaa catcttttga gagtatatca atgacaactg aagaggaaaa agtcgacagt
     4681 actttaaaaa gcgaaatgca aaatcgtgtc tctaagcctt cttttgatac ctggtttaaa
     4741 aacactaaga tcaaaattga aaataaaaat tgtttattac ttgtaccgag tgaatttgca
     4801 tttgaatgga ttaagaaaag atatttagaa acaattaaaa cagtccttga agaagctgga
     4861 tatgttttcg aaaaaatcga actaagaaaa gtgcaataaa ctgctgaagt atttcagcag
     4921 ttttttttat ttagaaatag tgaaaaaaat ataatcaggg aggtatcaat atttaatgag
     4981 tactgattta aatttattta gactggaatt aataattaac acgtagacta attaaaattt
     5041 aatgagggat aaagaggata caaaaatatt aatttcaatc cctattaaat tttaacaagg
     5101 gggggattaa aatttaatta gaggtttatc cacaagaaaa gaccctaata aaatttttac
     5161 tagggttata acactgatta atttcttaat gggggaggga ttaaaattta atgacaaaga
     5221 aaacaatctt ttaagaaaag cttttaaaag ataataataa aaagagcttt gcgattaagc
     5281 aaaactcttt actttttcat tgacattatc aaattcatcg atttcaaatt gttgttgtat
     5341 cataaagtta attctgtttt gcacaacctt ttcaggaata taaaacacat ctgaggcttg
     5401 ttttataaac tcagggtcgc taaagtcaat gtaacgtagc atatgatatg gtatagcttc
     5461 cacccaagtt agcctttctg cttcttctga atgtttttca tatacttcca tgggtatctc
     5521 taaatgattt tcctcatgta gcaaggtatg agcaaaaagt ttatggaatt gatagttcct
     5581 ctctttttct tcaacttttt tatctaaaac aaacacttta acatctgagt caatgtaagc
     5641 ataagatgtt tttccagtca taatttcaat cccaaatctt ttagacagaa attctggacg
     5701 taaatctttt ggtgaaagaa tttttttatg tagcaatata tccgatacag caccttctaa
     5761 aagcgttggt gaatagggca ttttacctat ctcctctcat tttgtggaat aaaaatagtc
     5821 atattcgtcc atctacctat cctattatcg aacagttgaa ctttttaatc aaggatcagt
     5881 cctttttttc attattctta aactgtgctc ttaactttaa caactcgatt tgtttttcca
     5941 gatctcgagg gtaactagcc tcgccgatcc cgcaagaggc ccggcagtca ggtggcactt
     6001 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6061 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6121 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6181 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6241 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6301 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6361 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6421 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6481 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6541 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6601 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6661 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6721 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6781 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     6841 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     6901 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     6961 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7021 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7081 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7141 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7201 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7261 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     7321 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7381 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7441 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7501 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7561 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7621 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7681 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7741 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7801 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     7861 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     7921 agcgcccaat acgcatgc
//
