LOCUS       V013195                 8037 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013195
VERSION     V013195
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8037
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      951..1539
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1852..2499)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     primer_bind     2974..2990
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(3036..4115)
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    4444..4468
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4613..4636
                     /label="8xHis"
                     /note="8xHis affinity tag"
     promoter        7852..7956
                     /label="AmpR promoter"
     CDS             join(7957..8037,1..777)
                     /label="AmpR"
                     /note="beta-lactamase"
ORIGIN
        1 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
       61 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
      121 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
      181 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
      241 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
      301 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
      361 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
      421 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
      481 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
      541 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
      601 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
      661 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
      721 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
      781 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
      841 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
      901 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
      961 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     1021 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     1081 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     1141 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     1201 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     1261 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     1321 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     1381 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     1441 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     1501 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     1561 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     1621 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     1681 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcatgctt
     1741 aagttattgg tatgactggt tttaagcgca aaaaaagttg ctttttcgta cctattaatg
     1801 tatcgttaga aaaccgactg taaaaagtac agtcggcatt atctcatatt ataaaagcca
     1861 gtcattaggc ctatctgaca attcctgaat agagttcata aacaatcctg catgataacc
     1921 atcacaaaca gaatgatgta cctgtaaaga tagcggtaaa tatattgaat tacctttatt
     1981 aatgaatttt cctgctgtaa taatgggtag aaggtaatta ctattattat tgatatttaa
     2041 gttaaaccca gtaaatgaag tccatggaat aatagaaaga gaaaaagcat tttcaggtat
     2101 aggtgttttg ggaaacaatt tccccgaacc attatatttc tctacatcag aaaggtataa
     2161 atcataaaac tctttgaagt cattctttac aggagtccaa ataccagaga atgttttaga
     2221 tacaccatca aaaattgtat aaagtggctc taacttatcc caataaccta actctccgtc
     2281 gctattgtaa ccagttctaa aagctgtatt tgagtttatc acccttgtca ctaagaaaat
     2341 aaatgcaggg taaaatttat atccttcttg ttttatgttt cggtataaaa cactaatatc
     2401 aatttctgtg gttatactaa aagtcgtttg ttggttcaaa taatgattaa atatctcttt
     2461 tctcttccaa ttgtctaaat caattttatt aaagttcatt tgatatgcct cctaaatttt
     2521 tatctaaagt gaatttagga ggcttacttg tctgctttct tcattagaat caatcctttt
     2581 ttaaaagtca atattactgt aacataaata tatattttaa aaatatccca ctttatccaa
     2641 ttttcgtttg ttgaactaat gggtgcttta gttgaagaat aaaagaccac attaaaaaat
     2701 gtggtctttt gtgttttttt aaaggatttg agcgtagcga aaaatccttt tctttcttat
     2761 cttgataata agggtaacta ttgccgtcgt ccattccgac agcatcgcca gtcactatgg
     2821 cgtgctgcta gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     2881 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     2941 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattcgagc
     3001 tcaggcctta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3061 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3121 ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc
     3181 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa
     3241 tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat
     3301 cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg
     3361 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc
     3421 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc
     3481 ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag
     3541 acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc
     3601 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg
     3661 tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca
     3721 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg
     3781 tgcaccgccg ttttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg
     3841 gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg
     3901 gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc
     3961 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc
     4021 gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca
     4081 tactctgcga catcgtataa cgttactggt ttcatcaaaa tcgtctccct ccgtttgaat
     4141 atttgattga tcgtaaccag atgaagcact ctttccacta tccctacagt gttatggctt
     4201 gaacaatcac gaaacaataa ttggtacgta cgatctttca gccgactcaa acatcaaatc
     4261 ttacaaatgt agtctttgaa agtattacat atgtaagatt taaatgcaac cgttttttcg
     4321 gaaggaaatg atgacctcgt ttccaccgga attagcttgg taccaaagga ggtaaggatc
     4381 actagaaaat tttttaaaaa atctcttgac attggaaggg agatatgtta ttataagaat
     4441 tgcggaattg tgagcggata acaattccca tataaaggag gaaggatcaa tgattcaaaa
     4501 acgaaagcgg acagtttcgt tcagacttgt gcttatgtgc acgctgttat ttgtcagttt
     4561 gccgattaca aaagcatcag ctgctggatc catgtctaga gtcgacgtcg ctcatcacca
     4621 tcaccatcac catcactaac gtccccgggg cagcccgcct aatgagcggg cttttttcac
     4681 gtcacgcgtc catggagatc tttgtctgca actgaaaagt ttatacctta cctggaacaa
     4741 atggttgaaa catacgaggc taatatcggc ttattaggaa tagtccctgt actaataaaa
     4801 tcaggtggat cagttgatca gtatattttg gacgaagctc ggaaagaatt tggagatgac
     4861 ttgcttaatt ccacaattaa attaagggaa agaataaagc gatttgatgt tcaaggaatc
     4921 acggaagaag atactcatga taaagaagct ctaaaactat tcaataacct tacaatggaa
     4981 ttgatcgaaa gggtggaagg ttaatggtac gaaaattagg ggatctacct agaaagccac
     5041 aaggcgatag gtcaagctta aagaaccctt acatggatct tacagattct gaaagtaaag
     5101 aaacaacaga ggttaaacaa acagaaccaa aaagaaaaaa agcattgttg aaaacaatga
     5161 aagttgatgt ttcaatccat aataagatta aatcgctgca cgaaattctg gcagcatccg
     5221 aagggaattc atattactta gaggatacta ttgagagagc tattgataag atggttgaga
     5281 cattacctga gagccaaaaa actttttatg aatatgaatt aaaaaaaaga accaacaaag
     5341 gctgagacag actccaaacg agtctgtttt tttaaaaaaa atattaggag cattgaatat
     5401 atattagaga attaagaaag acatgggaat aaaaatattt taaatccagt aaaaatatga
     5461 taagattatt tcagaatatg aagaactctg tttgtttttg atgaaaaaac aaacaaaaaa
     5521 aatccaccta acggaatctc aatttaacta acagcggcca aactgagaag ttaaatttga
     5581 gaaggggaaa aggcggattt atacttgtat ttaactatct ccattttaac attttattaa
     5641 accccataca agtgaaaatc ctcttttaca ctgttccttt aggtgatcgc ggagggacat
     5701 tatgagtgaa gtaaacctaa aaggaaatac agatgaatta gtgtattatc gacagcaaac
     5761 cactggaaat aaaatcgcca ggaagagaat caaaaaaggg aaagaagaag tttattatgt
     5821 tgctgaaacg gaagagaaga tatggacaga agagcaaata aaaaactttt ctttagacaa
     5881 atttggtacg catatacctt acatagaagg tcattataca atcttaaata attacttctt
     5941 tgatttttgg ggctattttt taggtgctga aggaattgcg ctctatgctc acctaactcg
     6001 ttatgcatac ggcagcaaag acttttgctt tcctagtcta caaacaatcg ctaaaaaaat
     6061 ggacaagact cctgttacag ttagaggcta cttgaaactg cttgaaaggt acggttttat
     6121 ttggaaggta aacgtccgta ataaaaccaa ggataacaca gaggaatccc cgatttttaa
     6181 gattagacgt aaggttcctt tgctttcaga agaactttta aatggaaacc ctaatattga
     6241 aattccagat gacgaggaag cacatgtaaa gaaggcttta aaaaaggaaa aagagggtct
     6301 tccaaaggtt ttgaaaaaag agcacgatga atttgttaaa aaaatgatgg atgagtcaga
     6361 aacaattaat attccagagg ccttacaata tgacacaatg tatgaagata tactcagtaa
     6421 aggagaaatt cgaaaagaaa tcaaaaaaca aatacctaat cctacaacat cttttgagag
     6481 tatatcaatg acaactgaag aggaaaaagt cgacagtact ttaaaaagcg aaatgcaaaa
     6541 tcgtgtctct aagccttctt ttgatacctg gtttaaaaac actaagatca aaattgaaaa
     6601 taaaaattgt ttattacttg taccgagtga atttgcattt gaatggatta agaaaagata
     6661 tttagaaaca attaaaacag tccttgaaga agctggatat gttttcgaaa aaatcgaact
     6721 aagaaaagtg caataaactg ctgaagtatt tcagcagttt tttttattta gaaatagtga
     6781 aaaaaatata atcagggagg tatcaatatt taatgagtac tgatttaaat ttatttagac
     6841 tggaattaat aattaacacg tagactaatt aaaatttaat gagggataaa gaggatacaa
     6901 aaatattaat ttcaatccct attaaatttt aacaaggggg ggattaaaat ttaattagag
     6961 gtttatccac aagaaaagac cctaataaaa tttttactag ggttataaca ctgattaatt
     7021 tcttaatggg ggagggatta aaatttaatg acaaagaaaa caatctttta agaaaagctt
     7081 ttaaaagata ataataaaaa gagctttgcg attaagcaaa actctttact ttttcattga
     7141 cattatcaaa ttcatcgatt tcaaattgtt gttgtatcat aaagttaatt ctgttttgca
     7201 caaccttttc aggaatataa aacacatctg aggcttgttt tataaactca gggtcgctaa
     7261 agtcaatgta acgtagcata tgatatggta tagcttccac ccaagttagc ctttctgctt
     7321 cttctgaatg tttttcatat acttccatgg gtatctctaa atgattttcc tcatgtagca
     7381 aggtatgagc aaaaagttta tggaattgat agttcctctc tttttcttca acttttttat
     7441 ctaaaacaaa cactttaaca tctgagtcaa tgtaagcata agatgttttt ccagtcataa
     7501 tttcaatccc aaatctttta gacagaaatt ctggacgtaa atcttttggt gaaagaattt
     7561 ttttatgtag caatatatcc gatacagcac cttctaaaag cgttggtgaa tagggcattt
     7621 tacctatctc ctctcatttt gtggaataaa aatagtcata ttcgtccatc tacctatcct
     7681 attatcgaac agttgaactt tttaatcaag gatcagtcct ttttttcatt attcttaaac
     7741 tgtgctctta actttaacaa ctcgatttgt ttttccagat ctcgagggta actagcctcg
     7801 ccgatcccgc aagaggcccg gcagtcaggt ggcacttttc ggggaaatgt gcgcggaacc
     7861 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7921 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7981 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacg
//
