LOCUS       Exported                7953 bp DNA     circular SYN 21-JUL-2025
DEFINITION  Exported.
ACCESSION   V001452
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7953)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7953)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7953)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7953)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7953
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(114..761)
                     /gene="cat"
                     /label=Chloramphenicol acetyltransferase
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     primer_bind     1236..1252
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1298..2377)
                     /label=lacI
                     /note="lac repressor"
     promoter        2681..2800
                     /label=Pgrac
     protein_bind    2761..2785
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      2830..2855
                     /label=trp terminator
     CDS             2919..3182
                     /codon_start=1
                     /label=ParA family protein
                     /translation="MVETYEANIGLLGIVPVLIKSGGSVDQYILDEARKEFGDDLLNST
                     IKLRERIKRFDVQGITEEDTHDKEALKLFNNLTMELIERVEG"
     CDS             3880..4914
                     /codon_start=1
                     /label=helix-turn-helix domain-containing protein
                     /translation="MSEVNLKGNTDELVYYRQQTTGNKIARKRIKKGKEEVYYVAETEE
                     KIWTEEQIKNFSLDKFGTHIPYIEGHYTILNNYFFDFWGYFLGAEGIALYAHLTRYAYG
                     SKDFCFPSLQTIAKKMDKTPVTVRGYLKLLERYGFIWKVNVRNKTKDNTEESPIFKIRR
                     KVPLLSEELLNGNPNIEIPDDEEAHVKKALKKEKEGLPKVLKKEHDEFVKKMMDESETI
                     NIPEALQYDTMYEDILSKGEIRKEIKKQIPNPTTSFESISMTTEEEKVDSTLKSEMQNR
                     VSKPSFDTWFKNTKIKIENKNCLLLVPSEFAFEWIKKRYLETIKTVLEEAGYVFEKIEL
                     RKVQ"
     CDS             complement(5290..5796)
                     /codon_start=1
                     /label=ORF-2
                     /translation="MPYSPTLLEGAVSDILLHKKILSPKDLRPEFLSKRFGIEIMTGKT
                     SYAYIDSDVKVFVLDKKVEEKERNYQFHKLFAHTLLHEENHLEIPMEVYEKHSEEAERL
                     TWVEAIPYHMLRYIDFSDPEFIKQASDVFYIPEKVVQNRINFMIQQQFEIDEFDNVNEK
                     VKSFA"
     promoter        6030..6134
                     /label=AmpR promoter
     CDS             6135..6992
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      7166..7754
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa
       61 tgtatcgtta gaaaaccgac tgtaaaaagt acagtcggca ttatctcata ttataaaagc
      121 cagtcattag gcctatctga caattcctga atagagttca taaacaatcc tgcatgataa
      181 ccatcacaaa cagaatgatg tacctgtaaa gatagcggta aatatattga attaccttta
      241 ttaatgaatt ttcctgctgt aataatgggt agaaggtaat tactattatt attgatattt
      301 aagttaaacc cagtaaatga agtccatgga ataatagaaa gagaaaaagc attttcaggt
      361 ataggtgttt tgggaaacaa tttccccgaa ccattatatt tctctacatc agaaaggtat
      421 aaatcataaa actctttgaa gtcattcttt acaggagtcc aaataccaga gaatgtttta
      481 gatacaccat caaaaattgt ataaagtggc tctaacttat cccaataacc taactctccg
      541 tcgctattgt aaccagttct aaaagctgta tttgagttta tcacccttgt cactaagaaa
      601 ataaatgcag ggtaaaattt atatccttct tgttttatgt ttcggtataa aacactaata
      661 tcaatttctg tggttatact aaaagtcgtt tgttggttca aataatgatt aaatatctct
      721 tttctcttcc aattgtctaa atcaatttta ttaaagttca tttgatatgc ctcctaaatt
      781 tttatctaaa gtgaatttag gaggcttact tgtctgcttt cttcattaga atcaatcctt
      841 ttttaaaagt caatattact gtaacataaa tatatatttt aaaaatatcc cactttatcc
      901 aattttcgtt tgttgaacta atgggtgctt tagttgaaga ataaaagacc acattaaaaa
      961 atgtggtctt ttgtgttttt ttaaaggatt tgagcgtagc gaaaaatcct tttctttctt
     1021 atcttgataa taagggtaac tattgccgtc gtccattccg acagcatcgc cagtcactat
     1081 ggcgtgctgc tagcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     1141 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     1201 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaattcga
     1261 gctcaggcct taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1321 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1381 tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga ttgcccttca
     1441 ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa
     1501 aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg gtatcgtcgt
     1561 atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg
     1621 cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg ccctcattca
     1681 gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc cgttccgcta
     1741 tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc agacgcgccg
     1801 agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat gcgaccagat
     1861 gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg atgggtgtct
     1921 ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc acagcaatgg
     1981 catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc gcgagaagat
     2041 tgtgcaccgc cgttttacag gcttcgacgc cgcttcgttc taccatcgac accaccacgc
     2101 tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca
     2161 gggccagact ggaggtggca acgccaatca gcaacgactg tttgcccgcc agttgttgtg
     2221 ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc ttccactttt tcccgcgttt
     2281 tcgcagaaac gtggctggcc tggttcacca cgcgggaaac ggtctgataa gagacaccgg
     2341 catactctgc gacatcgtat aacgttactg gtttcatcaa aatcgtctcc ctccgtttga
     2401 atatttgatt gatcgtaacc agatgaagca ctctttccac tatccctaca gtgttatggc
     2461 ttgaacaatc acgaaacaat aattggtacg tacgatcttt cagccgactc aaacatcaaa
     2521 tcttacaaat gtagtctttg aaagtattac atatgtaaga tttaaatgca accgtttttt
     2581 cggaaggaaa tgatgacctc gtttccaccg gaattagctt ggtaccagct attgtaacat
     2641 aatcggtacg ggggtgaaaa agctaacgga aaagggagcg gaaaagaatg atgtaagcgt
     2701 gaaaaatttt ttatcttatc acttgaaatt ggaagggaga ttctttatta taagaattgt
     2761 ggaattgtga gcggataaca attcccaatt aaaggaggaa ggatcctcta gagtcgacgt
     2821 ccccggggca gcccgcctaa tgagcgggct tttttcacgt cacgcgtcca tggagatctt
     2881 tgtctgcaac tgaaaagttt ataccttacc tggaacaaat ggttgaaaca tacgaggcta
     2941 atatcggctt attaggaata gtccctgtac taataaaatc aggtggatca gttgatcagt
     3001 atattttgga cgaagctcgg aaagaatttg gagatgactt gcttaattcc acaattaaat
     3061 taagggaaag aataaagcga tttgatgttc aaggaatcac ggaagaagat actcatgata
     3121 aagaagctct aaaactattc aataacctta caatggaatt gatcgaaagg gtggaaggtt
     3181 aatggtacga aaattagggg atctacctag aaagccacaa ggcgataggt caagcttaaa
     3241 gaacccttac atggatctta cagattctga aagtaaagaa acaacagagg ttaaacaaac
     3301 agaaccaaaa agaaaaaaag cattgttgaa aacaatgaaa gttgatgttt caatccataa
     3361 taagattaaa tcgctgcacg aaattctggc agcatccgaa gggaattcat attacttaga
     3421 ggatactatt gagagagcta ttgataagat ggttgagaca ttacctgaga gccaaaaaac
     3481 tttttatgaa tatgaattaa aaaaaagaac caacaaaggc tgagacagac tccaaacgag
     3541 tctgtttttt taaaaaaaat attaggagca ttgaatatat attagagaat taagaaagac
     3601 atgggaataa aaatatttta aatccagtaa aaatatgata agattatttc agaatatgaa
     3661 gaactctgtt tgtttttgat gaaaaaacaa acaaaaaaaa tccacctaac ggaatctcaa
     3721 tttaactaac agcggccaaa ctgagaagtt aaatttgaga aggggaaaag gcggatttat
     3781 acttgtattt aactatctcc attttaacat tttattaaac cccatacaag tgaaaatcct
     3841 cttttacact gttcctttag gtgatcgcgg agggacatta tgagtgaagt aaacctaaaa
     3901 ggaaatacag atgaattagt gtattatcga cagcaaacca ctggaaataa aatcgccagg
     3961 aagagaatca aaaaagggaa agaagaagtt tattatgttg ctgaaacgga agagaagata
     4021 tggacagaag agcaaataaa aaacttttct ttagacaaat ttggtacgca tataccttac
     4081 atagaaggtc attatacaat cttaaataat tacttctttg atttttgggg ctatttttta
     4141 ggtgctgaag gaattgcgct ctatgctcac ctaactcgtt atgcatacgg cagcaaagac
     4201 ttttgctttc ctagtctaca aacaatcgct aaaaaaatgg acaagactcc tgttacagtt
     4261 agaggctact tgaaactgct tgaaaggtac ggttttattt ggaaggtaaa cgtccgtaat
     4321 aaaaccaagg ataacacaga ggaatccccg atttttaaga ttagacgtaa ggttcctttg
     4381 ctttcagaag aacttttaaa tggaaaccct aatattgaaa ttccagatga cgaggaagca
     4441 catgtaaaga aggctttaaa aaaggaaaaa gagggtcttc caaaggtttt gaaaaaagag
     4501 cacgatgaat ttgttaaaaa aatgatggat gagtcagaaa caattaatat tccagaggcc
     4561 ttacaatatg acacaatgta tgaagatata ctcagtaaag gagaaattcg aaaagaaatc
     4621 aaaaaacaaa tacctaatcc tacaacatct tttgagagta tatcaatgac aactgaagag
     4681 gaaaaagtcg acagtacttt aaaaagcgaa atgcaaaatc gtgtctctaa gccttctttt
     4741 gatacctggt ttaaaaacac taagatcaaa attgaaaata aaaattgttt attacttgta
     4801 ccgagtgaat ttgcatttga atggattaag aaaagatatt tagaaacaat taaaacagtc
     4861 cttgaagaag ctggatatgt tttcgaaaaa atcgaactaa gaaaagtgca ataaactgct
     4921 gaagtatttc agcagttttt tttatttaga aatagtgaaa aaaatataat cagggaggta
     4981 tcaatattta atgagtactg atttaaattt atttagactg gaattaataa ttaacacgta
     5041 gactaattaa aatttaatga gggataaaga ggatacaaaa atattaattt caatccctat
     5101 taaattttaa caaggggggg attaaaattt aattagaggt ttatccacaa gaaaagaccc
     5161 taataaaatt tttactaggg ttataacact gattaatttc ttaatggggg agggattaaa
     5221 atttaatgac aaagaaaaca atcttttaag aaaagctttt aaaagataat aataaaaaga
     5281 gctttgcgat taagcaaaac tctttacttt ttcattgaca ttatcaaatt catcgatttc
     5341 aaattgttgt tgtatcataa agttaattct gttttgcaca accttttcag gaatataaaa
     5401 cacatctgag gcttgtttta taaactcagg gtcgctaaag tcaatgtaac gtagcatatg
     5461 atatggtata gcttccaccc aagttagcct ttctgcttct tctgaatgtt tttcatatac
     5521 ttccatgggt atctctaaat gattttcctc atgtagcaag gtatgagcaa aaagtttatg
     5581 gaattgatag ttcctctctt tttcttcaac ttttttatct aaaacaaaca ctttaacatc
     5641 tgagtcaatg taagcataag atgtttttcc agtcataatt tcaatcccaa atcttttaga
     5701 cagaaattct ggacgtaaat cttttggtga aagaattttt ttatgtagca atatatccga
     5761 tacagcacct tctaaaagcg ttggtgaata gggcatttta cctatctcct ctcattttgt
     5821 ggaataaaaa tagtcatatt cgtccatcta cctatcctat tatcgaacag ttgaactttt
     5881 taatcaagga tcagtccttt ttttcattat tcttaaactg tgctcttaac tttaacaact
     5941 cgatttgttt ttccagatct cgagggtaac tagcctcgcc gatcccgcaa gaggcccggc
     6001 agtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     6061 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     6121 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     6181 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     6241 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     6301 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     6361 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     6421 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     6481 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     6541 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     6601 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     6661 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     6721 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     6781 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     6841 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     6901 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     6961 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     7021 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     7081 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     7141 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     7201 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     7261 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     7321 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     7381 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     7441 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     7501 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     7561 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     7621 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     7681 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     7741 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     7801 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     7861 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     7921 gcgaggaagc ggaagagcgc ccaatacgca tgc
//
