LOCUS       V005484                 7398 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005484
VERSION     V005484
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Ben Rejeb S, Lereclus D, Slamti L.
  TITLE     Analysis of abrB Expression during the Infectious Cycle of Bacillus
            thuringiensis Reveals Population Heterogeneity
  JOURNAL   Front Microbiol 8, 2471 (2017)
   PUBMED   29312181
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Ben Rejeb S, Slamti L.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-AUG-2017) MICA-Institut Micalis, INRA, Domaine de
            Vilvert, Jouy-en-Josas 78350, France
REFERENCE   3  (bases 1 to 7398)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7398)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front
            Microbiol 8, 2471 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (16-AUG-2017) MICA-Institut Micalis, INRA, Domaine de Vilvert,
            Jouy-en-Josas 78350, France"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7398
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             49..57
                     /label="Shine-Dalgarno sequence"
                     /note="full consensus sequence for ribosome-binding sites
                     upstream of start codons in E. coli; complementary to a
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             88..801
                     /label="superfolder GFP"
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Nager et al., 2011)"
     primer_bind     complement(887..903)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(911..927)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(935..965)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(980..1001)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1289..1877)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2051..2908)
                     /label="AmpR"
                     /note="beta-lactamase"
     CDS             complement(3071..3805)
                     /gene="erm"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Streptococcus pneumoniae. Accession#: P21236"
     primer_bind     7344..7360
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 gggggcgcgc cgaaataaaa tgcatctgta tttgaatgaa tttattttta aggaggtgac
       61 accatgaatg ggattgaaat ttttgcgatg tcaaaaggag aggaattgtt tacgggtgtc
      121 gtaccgatct tggtggagct agatggagac gttaatggtc acaagtttag tgtacgtggg
      181 gaaggtgaag gagacgctac taatgggaaa ttgactttaa aatttatttg tactacgggg
      241 aaactgccgg ttccctggcc gacactagtt acgacattaa cttacggtgt tcaatgtttt
      301 tcaagatatc ctgatcatat gaaaaggcat gacttcttca aatcagcgat gccagagggt
      361 tatgttcaag agagaactat atcgtttaaa gatgatggca catataaaac acgcgctgaa
      421 gtgaagtttg aaggagatac acttgtgaac cgtattgagc ttaaaggaat tgattttaaa
      481 gaagatggaa acattctagg tcacaagctt gaatataact ttaactctca taatgtgtat
      541 ataacagcag ataagcagaa gaatggtatt aaagcaaatt ttaaaattag acataatgtg
      601 gaagatggtt ctgtacaatt agctgatcat tatcaacaaa atacacctat aggtgatggt
      661 cctgtattat taccagataa tcattattta agtacacaaa gtgtattaag taaggatcca
      721 aatgaaaaac gagatcatat ggtattatta gaatttgtaa cagcagcagg tataacacat
      781 ggtatggatg aattatataa aggatctcat catcatcatc atggcaaaca aaacaatctt
      841 tctgcagcag tataaggtac cccagctcga attcggcgta atcatggtca tagctgtttc
      901 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
      961 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
     1021 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     1081 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     1141 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     1201 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     1261 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     1321 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     1381 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     1441 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     1501 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     1561 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     1621 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     1681 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     1741 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     1801 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     1861 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     1921 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     1981 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     2041 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     2101 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     2161 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     2221 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     2281 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     2341 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
     2401 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     2461 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     2521 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     2581 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     2641 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
     2701 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     2761 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     2821 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     2881 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatgtacc catcgcgata
     2941 caaattcccc gtaggcgcta gggacctctt tagcttcttg gaagctgtca gtagtatatc
     3001 taataattta tctccattcc ctttagtaac gtgtaacttt ccaaatttaa aaaagcgact
     3061 catagaatta tttcctcccg ttaaataata gataactatt aaaaatagac aatacttgct
     3121 cataagtaat ggtacttaaa ttgtttactt tggcgtgttt cattgcttga tgaaactgat
     3181 ttttagtaaa cagttgacga tattctcgat tgacccattt tgaaacaaag tacgtatata
     3241 gcttccaata tttatctgga acatctgtgg tatggcgggt aagttttatt aagacactgt
     3301 ttacttttgg tttaggatga aagcattccg ctggcagctt aagcaattgc tgaatcgaga
     3361 cttgagtgtg caagagcaac cctagtgttc ggtgaatatc caaggtacgc ttgtagaatc
     3421 cttcttcaac aatcagatag atgtcagacg catggctttc aaaaaccact tttttaataa
     3481 tttgtgtgct taaatggtaa ggaatactcc caacaatttt atacctctgt ttgttaggga
     3541 attgaaactg tagaatatct tggtgaatta aagtgacacg aatgttcagt tttaattttt
     3601 ctgacgataa gttgaataga tgactgtcta attcaataga cgttacctgt ttacttattt
     3661 tagccagttt cgtcgttaaa tgccctttac ctgttccaat ttcgtaaacg gtatcggttt
     3721 cttttaaatt caattgtttt attatttggt tgagtacttt ttcactcgtt aaaaagtttt
     3781 gagaatattt tatatttttg ttcatgtaat tactcctgaa gtgattacat ctgtaaataa
     3841 atacagaagt taaacgattt gtttgtaatt ttagttatct gtttaaaaag tcataagatt
     3901 agtcactggt aggaattaat ctaacgtatt tatttatctg cgtaatcact gtttttagtc
     3961 tgtttcaaaa cagtagatgt tttatctaca ttacgcattt ggaataccaa catgacgaat
     4021 ccctccttct taattacaaa tttttagcat ctaatttaac ttcaattcct attatacaca
     4081 aaattttaag atactgcact atcaacacac tcttaagttt gcttctaagg gggatcaaca
     4141 tattagatat cgtggtgttc aaaaacgcta cctcatgatc ctgcaatgat ataactttat
     4201 cttgcaaatc ctttacgaca tcagtagatg gcgccgctgt aaatagtatc cccagcatca
     4261 atagttcccc ctgattatat aaattcagac gaatactatt atccattatt tttttattaa
     4321 ggtaaattaa tattttacct attaaaggag ggacgattta agtatggacg tgatgtttgg
     4381 tcaaggtggg aacataaatt ttaattacat tggaacatat tgtggacaag catctaattc
     4441 ataatcccca ctttccaagc atgaaagtcc cttataccat caattattca ataaaatatc
     4501 cctatcataa acctgtcccg cgcgacggtt caaatactcc tttatgataa aataaagata
     4561 taaagagacg agatgaggga gaaatttgac ggctaataaa aaaaagattg taaataaacc
     4621 aactgttgtg gcaaaacttc cacaaatcat tatagataca tataacttga cttgcacttc
     4681 taacgatgta aaaatgtttc ccggatttat aaagcacgta aagaaacaac atcctggtat
     4741 ttatgaaaaa tattcatcgc acataaaaga tatcgttgaa caccccgact atgtcgggca
     4801 aaatcctaaa gaacctaata gcgttgaatt ggtaaagatt ttaaatgacc atatactaat
     4861 tgctataaag ttggacccaa gcggttattt gttcttatca actatgttcg atctaaaaaa
     4921 cggtcctgcg aaaatccaaa gacgattgaa tagtggacgt ttaattgctt acaaagacct
     4981 gttaagctaa cggggagttt actaaaatat aagaacatgg tatactacct gtagaaagta
     5041 acataataat gatataggat ctttgaggtg ggaaaggttc cccacgcgct tatctaaccg
     5101 ccgttctcaa gcatttgatt ggctaggtaa gttaccagag atgtaggata cgccgcccta
     5161 ccaacgatcc tatgtacggt aagccatccg ttttttacgg atggcttttt atattgactt
     5221 tttcatcgct aatggttact tttctatcaa acgacaccga aaggcgatga ttattgtgtt
     5281 aaaaaaccca atattataac atccactaat ggataatctt acttttagta agtaggttgc
     5341 ctagtgaaat acaataattc caacattggg ccttaataca tattaattag ttttaaccct
     5401 ctcactataa aagagttaaa tatgactccg caacctcttt aatatcacta ttacattttg
     5461 taacaacatt attactttca tcctctgtac cattaacaaa taaatgagga ttctttacca
     5521 taaagtctaa caatagatca ataaaccgct gcctttgttc tctagccatt tcctttctca
     5581 tagagaacca ctcccctatc tttcactatt cttttaattt catcttgtct ttcacttgat
     5641 ttaaacagct ctggattctt cctcaatccc ccactatctt agttaatggt agttgcttca
     5701 gtactctaca ttttttgcta atcgaggtta aaatccttca aatcccttga ctttaaggtc
     5761 ttaaggtttt catactggcg agtttgttag gaaatctgaa tgtagttttg ggcatagtaa
     5821 cccctgttga aaatttacga ggttaaaacg cccactcatt cgtactggct tagatgccga
     5881 ggtctcgaat tgagattggt tgcattgacg acaaacggtt atagcttaca aaactactat
     5941 accgtccgta tatagcttat tggcattgta ttgcgccgta cggtgcgttt ctcacgccca
     6001 acaccgttcc ctttcgggtg attcgctata taacccgcat atccttgtaa taaagctagc
     6061 ttgtacaaga atagccgtga atcagtgtca tgatcgccgc ttacgagtaa ctgtttaact
     6121 ccagtcgcac aatcatgtct tattttgaac ccccaacact ttttgccgct aacgtcttgg
     6181 ttatcatcat tattaggatt tacatcccgt aggcttgttt tatgcaaggc ttgtgtcctt
     6241 tacctcgctt acgtcagaca gttgaatgca acgacaactg atatccgagt gtactctcct
     6301 gcattggcta cactagacca tctttttaaa gtggtagctt ccactggaac ggaacacaaa
     6361 aagggcgttc tctatatcta aacgccctgt acatttacaa gacttctagg tatagaaaac
     6421 gcccttttat atccgtttta ttatgtatat gacaatgcta gtaattacta gttgaaatat
     6481 tcgtagagta acggtacaat aggtgtatct aataagcctt gttcgcgaaa acaaggcaaa
     6541 taacttattt gaggttggcg cctcttataa gtcgaagtcc tgtatggctg tacaggatta
     6601 agtcattctc gctaaaggtt ggtagccaat agcatatgag agtggctttt ttcatttccg
     6661 ttctattaag ttactgctaa ttttaccgct atgtttctat taaatcaacg aaaatttgat
     6721 aaggttcacc agaaacatta atatttgcga cataaaactc actctccaac tttggaggat
     6781 ggattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     6841 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     6901 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     6961 tcgtctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
     7021 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
     7081 gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag
     7141 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     7201 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     7261 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     7321 agggttttcc cagtcacgac gttgtaaaac gacggccagt aagcttgcat gcctgcaggt
     7381 cgactctaga ggatcccc
//
