LOCUS       40924_25116        4344 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHSP-MCS-mGFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Saidi Y, Moody LA, Coates JC.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-MAR-2015) Department of Biosciences, University of 
            Birmingham, Edgbaston, Birmingham B15 2TT, UK
REFERENCE   2  (bases 1 to 4344)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4344)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted 
            (07-MAR-2015) Department of Biosciences, University of Birmingham, 
            Edgbaston, Birmingham B15 2TT, UK"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4344
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      21..521
                     /label=Gmhsp17.5
                     /note="Gmhsp17.5"
                     /regulatory_class="promoter"
     misc_feature    522..557
                     /label=MCS
                     /note="MCS"
     CDS             558..1271
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
     regulatory      1281..1475
                     /label=35S Ter
                     /note="35S Ter"
                     /regulatory_class="terminator"
     promoter        complement(1490..1508)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1518..1534)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1675..2130)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2157..2261
                     /label=AmpR promoter
     CDS             2262..3119
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3293..3881
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    4169..4190
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4205..4235
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4243..4259
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4267..4283
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4304..4322
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gcggtggcgg ccgctctaga tagtcagcct tttaagagat agaatttaaa atataatttg
       61 cgtaaaacat tattaaaaat acaaatttat aaattaagtt caactcatcc tatctcactc
      121 tttaaatacg atgtttactt attagactca ttaataaaaa aaaaaaaatc atttgtacaa
      181 agcccaccat aaaggcaatt tgggcctggt agaccaatcc taaccaatgt ctggttaaga
      241 tggtccaatc ccgaaacttc tagttgcggt tcgaagaagt ccagaatgtt tctgaaagtt
      301 tcagaaaatt ctagttttga gattttcaga agtacggcat gatgatgcat aacaaggact
      361 ttctcgaaag tactatattg ctcctctaca tcattttaaa taccccatgt gtcctttgaa
      421 gacacatcac agaaagaagt gaaggcatcg ttagcagttt tgtagattca acctcaattt
      481 gcagagttac gttctaatat atttacacaa gactgataag aggatccctc gagcccggga
      541 agcttgtcga cactagtatg agtaaaggag aagaactttt cactggagtt gtcccaattc
      601 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      661 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      721 ttccgtggcc aacacttgtc actactttct cttatggtgt tcaatgcttt tcaagatacc
      781 cagatcatat gaagcggcac gacttcttca agagcgccat gcctgaggga tacgtgcagg
      841 agaggaccat cttcttcaag gacgacggga actacaagac acgtgctgaa gtcaagtttg
      901 agggagacac cctcgtcaac aggatcgagc ttaagggaat cgatttcaag gaggacggaa
      961 acatcctcgg ccacaagttg gaatacaact acaactccca caacgtatac atcatggccg
     1021 acaagcaaaa gaacggcatc aaagccaact tcaagacccg ccacaacatc gaagacggcg
     1081 gcgtgcaact cgctgatcat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1141 taccagacaa ccatcacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     1201 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     1261 aactatacaa ataagaattc cgctgaaatc accagtctct ctctacaaat ctatctctct
     1321 ctataataat gtgtgagtag ttcccagata agggaattag ggttcttata gggtttcgct
     1381 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
     1441 aataaaattt ctaattccta aaaccaaaat ccaggggtac ccaattcgcc ctatagtgag
     1501 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     1561 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     1621 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggaaattg
     1681 taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc tcatttttta
     1741 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     1801 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     1861 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     1921 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     1981 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2041 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2101 ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg
     2161 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     2221 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     2281 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     2341 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     2401 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     2461 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     2521 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     2581 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     2641 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     2701 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     2761 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     2821 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     2881 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     2941 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     3001 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     3061 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3121 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3181 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     3241 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     3301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     3361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     3421 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     3481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     3541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     3601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     3661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     3721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     3781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     3841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     3901 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     3961 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     4021 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     4081 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     4141 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     4201 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     4261 ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
     4321 gggaacaaaa gctggagctc cacc
//
