LOCUS       pHSG396.        2238 bp DNA     circular SYN 01-JAN-1980
DEFINITION  pUC-type bacterial cloning vector with a chloramphenicol resistance 
            gene. The MCS is similar but reversed in pHSG398.
ACCESSION   .
VERSION     .
KEYWORDS    pHSG396
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Takeshita S, Sato M, Toba M, Masahashi W, Hashimoto-Gotoh T.
  TITLE     High-copy-number and low-copy-number plasmid vectors for lacZ 
            alpha-complementation and chloramphenicol- or kanamycin-resistance 
            selection.
  JOURNAL   Gene 1987;61:63-74.
  PUBMED    3327753
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   TaKaRa
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 2238)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1987"; volume: "61"; pages: "63-74"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        90..192
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             193..849
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     protein_bind    1059..1080
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1095..1125
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1133..1149
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1157..1173
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    1186..1254
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     primer_bind     complement(1255..1271)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(1597..2185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac cgggaagccc
       61 tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc caactttcac
      121 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
      181 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
      241 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
      301 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
      361 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt tcgtatggca
      421 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
      481 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
      541 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
      601 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
      661 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
      721 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtctgt
      781 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
      841 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct
      901 gaataagtga taataagcgg atgaatggca gaaattcagc ttggcccagt gccaagctca
      961 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     1021 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     1081 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     1141 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcccc
     1201 tcgaggggtc gactctagag gatcccccat cgatggggta ccgagctcga attcactggc
     1261 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     1321 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     1381 ccaacagttg cgcagcctga atggcgaatg agcttcttcc gcttcctcgc tcactgactc
     1441 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     1501 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     1561 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     1621 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     1681 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     1741 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg
     1801 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     1861 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     1921 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     1981 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
     2041 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     2101 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     2161 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     2221 tcagtggaac tccgtcga
//
