LOCUS       V001133                14914 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V001133
VERSION     V001133
KEYWORDS    pHRdSV40-NLS-dCas9-24xGCN4_v4-NLS-P2A-BFP-dWPRE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14914)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi:
            10.1016/j.cell.2014.09.039.
   PUBMED   25307933
REFERENCE   2  (bases 1 to 14914)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 14914)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        90..419
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     intron          1553..1618
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1748..1768
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    2193..2327
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             2495..3128
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3175..3300
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    3797..4030
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             4215..4259
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             4408..4449
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    4544..4661
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        4724..4934
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     rep_origin      4793..4928
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             4950..4970
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             5025..9128
                     /label="dCas9"
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             9132..9158
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label="HA"
                     /translation="YPYDVPDYA"
     CDS             9177..9197
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             9204..9224
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             9303..9359
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9375..9431
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9447..9503
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9519..9575
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9591..9647
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9663..9719
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9735..9791
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9807..9863
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9879..9935
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             9951..10007
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10023..10079
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10095..10151
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10167..10223
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10239..10295
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10311..10367
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10383..10439
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10455..10511
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10527..10583
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10599..10655
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10671..10727
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10743..10799
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10815..10871
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10959..11015
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility
                     while preserving binding to an scFv-GFP fusion protein
                     (Tanenbaum et al., 2014)"
                     /label="GCN4_v4"
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11055..11075
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             11085..11141
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             11154..11852
                     /label="TagBFP"
                     /note="monomeric blue fluorescent protein"
     LTR             11970..12203
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(12228..12246)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(12536..12555)
                     /label="pBRrevBam"
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"
     primer_bind     complement(12769..12788)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     12888..12910
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12948..12966)
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     promoter        13034..13138
                     /label="AmpR promoter"
     CDS             13139..13996
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      14170..14758
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
       61 tggccgattc attaatgcag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttggaca caagacaggc ttgcgagata tgtttgagaa taccacttta tcccgcgtca
      481 gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt tagtgcgcca
      541 gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc cgtagataaa
      601 caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat gttgcagatc
      661 catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg cattattgcc
      721 gtaagccgtg gcggtctgta ccgggtgcgt tactggcgcg tgaactgggt attcgtcatg
      781 tcgataccgt ttgtatttcc agctacgatc acgacaacca gcgcgagctt aaagtgctga
      841 aacgcgcaga aggcgatggc gaaggcttca tcgttattga tgacctggtg gataccggtg
      901 gtactgcggt tgcgattcgt gaaatgtatc caaaagcgca ctttgtcacc atcttcgcaa
      961 aaccggctgg tcgtccgctg gttgatgact atgttgttga tatcccgcaa gatacctgga
     1021 ttgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc aatctccggt cgctaatctt
     1081 ttcaacgcct ggcactgccg ggcgttgttc tttttaactt caggcgggtt acaatagttt
     1141 ccagtaagta ttctggaggc tgcatccatg acacaggcaa acctgagcga aaccctgttc
     1201 aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc gccgctttaa tcacggcgca
     1261 caaccgcctg tgcagtcggc ccttgatggt aaaaccatcc ctcactggta tcgcatgatt
     1321 aaccgtctga tgtggatctg gcgcggcatt gacccacgcg aaatcctcga cgtccaggca
     1381 cgtattgtga tgagcgatgc cgaacgtacc gacgatgatt tatacgatac ggtgattggc
     1441 taccgtggcg gcaactggat ttatgagtgg gccccggatc tttgtgaagg aaccttactt
     1501 ctgtggtgtg acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat
     1561 aaaattttta agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat
     1621 tccaacctat ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct
     1681 gttttgctca gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc
     1741 tactcctcca aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct
     1801 aagttttttg agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac
     1861 cacaaaggaa aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt
     1921 tataagtagg cataacagtt ataatcataa catactgttt tttcttactc cacacaggca
     1981 tagagtgtct gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg
     2041 taaaggggtt aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc
     2101 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
     2161 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
     2221 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2281 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc
     2341 aagctaacca aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc
     2401 tgtggtttca tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt
     2461 gtatttgtta aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga
     2521 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag
     2581 aactacacac cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta
     2641 gtaccagttg agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta
     2701 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt
     2761 gacagccgcc tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac
     2821 tgctgatatc gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc
     2881 ctgggcggga ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc
     2941 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3001 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3061 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3121 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     3181 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     3241 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     3301 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     3361 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     3421 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     3481 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3541 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3601 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccggccgctg atcttcagac
     3661 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     3721 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     3781 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     3841 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     3901 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     3961 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     4021 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     4081 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     4141 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     4201 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     4261 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     4321 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     4381 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     4441 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     4501 acggatctcg acggtatcgc caaatggcag tattcatcca caattttaaa agaaaagggg
     4561 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     4621 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     4681 gcagagatcc agtttggatc gataagcttg atatcgaatt caggcaggca gaagtatgca
     4741 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     4801 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4861 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     4921 tggaggccta ggctacgcgc gccaccatgc ccaagaagaa gcgcaaggtg ggacgcgtct
     4981 gcaggatatc aagcttgcgg taccgcgggc ccgggatcgc caccatggac aagaagtaca
     5041 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     5101 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     5161 acctgatcgg cgccctgctg ttcgacagcg gagaaacagc cgaggccacc cggctgaaga
     5221 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     5281 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     5341 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     5401 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     5461 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     5521 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     5581 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     5641 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     5701 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg caacctgatt gccctgagcc
     5761 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     5821 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     5881 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     5941 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     6001 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     6061 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac atcgatggcg
     6121 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     6181 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     6241 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     6301 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     6361 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     6421 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     6481 ccagcgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     6541 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtacaac gagctgacca
     6601 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     6661 aagccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     6721 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     6781 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     6841 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     6901 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     6961 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     7021 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     7081 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     7141 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     7201 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     7261 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     7321 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     7381 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     7441 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     7501 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct
     7561 ttctgaagga cgactccatc gataacaaag tgctgactcg gagcgacaag aaccggggca
     7621 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcgccagc
     7681 tgctgaatgc caagctgatt acccagagga agttcgacaa tctgaccaag gccgagagag
     7741 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     7801 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     7861 acgacaaact gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     7921 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     7981 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     8041 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     8101 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     8161 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     8221 acggcgaaac aggcgagatc gtgtgggata agggccggga ctttgccacc gtgcggaaag
     8281 tgctgtctat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     8341 gcaaagagtc tatcctgccc aagaggaaca gcgacaagct gatcgccaga aagaaggact
     8401 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     8461 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     8521 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     8581 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     8641 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     8701 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     8761 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aaacactacc
     8821 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     8881 atctggacaa ggtgctgagc gcctacaaca agcacagaga caagcctatc agagagcagg
     8941 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
     9001 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
     9061 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
     9121 gaggcgacgc ctatccctat gacgtgcccg attatgccag cctgggcagc ggctccccca
     9181 agaaaaaacg caaggtggaa gatcctaaga aaaagcggaa agtggacggc attggtagtg
     9241 ggagcaacgg cagcagcgga tccaacggtc cgggtggatc tggaggtgga ggttctggag
     9301 gagaagaact tttgagcaag aattatcatc ttgagaacga agtggctcgt cttaagaaag
     9361 gttctggcag tggagaagaa ctgctttcaa agaattacca cctggaaaat gaggtagcta
     9421 gactgaaaaa ggggagcgga agtggggagg agttgctgag caaaaattat catttggaga
     9481 acgaagtagc acgactaaag aaagggtccg gatcgggtga ggagttactc tcgaaaaatt
     9541 atcatctcga aaacgaagtg gctcggctaa aaaagggcag tggttctgga gaagagctat
     9601 tatctaaaaa ctaccacctc gaaaatgagg tggcacgctt aaaaaaggga agtggcagtg
     9661 gtgaagagct actatccaag aattatcatc ttgagaacga ggtagcgcgt ttgaagaagg
     9721 gttccggctc aggagaggaa ctgctctcga agaactatca tcttgaaaat gaggtcgctc
     9781 gattaaaaaa gggatcgggc agtggtgagg aactactttc aaagaattac cacctcgaaa
     9841 acgaagtagc tcgattaaag aaaggttcag ggtcgggtga agaattactg agtaaaaatt
     9901 atcatctgga aaatgaggta gcgagactaa aaaaggggag tggttctggc gaagagttgc
     9961 tatcgaaaaa ttatcatctt gagaacgaag ttgctaggct caaaaagggc tcaggctcag
    10021 gcgaggagtt gctctcgaaa aactaccact tggaaaatga ggtcgcgagg ttgaaaaagg
    10081 ggagcgggtc gggcgaggag ttattgagca aaaactatca tttagagaac gaagtcgcgc
    10141 gcttaaagaa aggctcgggc tcgggcgaag aactcttatc gaagaactac cacctcgaaa
    10201 atgaggtcgc caggttgaaa aagggcagtg gcagcgggga ggaactcttg agcaagaact
    10261 accacttgga gaatgaggtc gcgagattga agaaagggtc ggggagcggc gaggaattgc
    10321 tcagcaagaa ttatcatttg gagaacgaag tcgccaggct caagaaaggc tcggggtcgg
    10381 gggaggaatt gttgagtaaa aactaccact tggaaaatga agtcgccagg ctcaaaaaag
    10441 ggagtgggag cggcgaagag ttattgagca aaaattacca cttggagaac gaagtggcaa
    10501 ggctcaagaa agggagcggc agcggggagg agctcttatc gaagaactac cacttagaga
    10561 atgaagtcgc ccgcttgaag aaaggctcgg ggagcgggga agagctcttg agcaagaact
    10621 accacttgga aaatgaggtg gcgcgcttga agaaagggag cgggagcggg gaagagttac
    10681 tatctaagaa ttatcatctc gagaacgagg tggctcgact aaagaagggc tccggcagtg
    10741 gggaggaact cctgtcgaag aactatcatc ttgaaaatga ggttgcaaga cttaaaaagg
    10801 ggtccggatc aggtgaggaa ctactcagta agaattacca cctggaaaac gaagttgcac
    10861 gtttgaagaa aggatcagga tcaggcgaag aactgctctc aaaagattat catttggaaa
    10921 atgaggttgc acgtttaaaa aagggaagtg gcagtggtga ggaacttctg tcgaaaaatt
    10981 atcatctcga gaatgaagta gcccgactta aaaagggttc tggctcgggt cagcggccgc
    11041 aaggtggagg tggacccaag aagaagcgca aggtgggaag cggagctact aacttcagcc
    11101 tgctgaagca ggctggagac gtggaggaga accctggacc tgcaccagga agtatgagcg
    11161 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
    11221 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
    11281 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
    11341 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
    11401 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
    11461 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
    11521 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
    11581 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
    11641 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
    11701 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
    11761 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
    11821 acctccctag caaactgggg cacaagctta attaagcggc cgcgactcta gagtcgacct
    11881 gcagggatcg atcactgagg tacctttaag accaatgact tacaaggcag ctgtagatct
    11941 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
    12001 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
    12061 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    12121 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    12181 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac
    12241 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac
    12301 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc
    12361 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc
    12421 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc
    12481 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
    12541 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
    12601 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
    12661 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
    12721 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc
    12781 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
    12841 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
    12901 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
    12961 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
    13021 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    13081 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    13141 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    13201 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    13261 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    13321 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    13381 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    13441 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    13501 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    13561 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    13621 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    13681 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    13741 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    13801 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    13861 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    13921 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    13981 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    14041 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    14101 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    14161 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
    14221 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
    14281 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
    14341 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
    14401 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
    14461 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
    14521 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
    14581 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
    14641 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
    14701 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
    14761 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
    14821 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
    14881 taccgctcgc cgcagccgaa cgaccgagcg cagc
//
