LOCUS       V012212                11269 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V012212
VERSION     V012212
KEYWORDS    pHR_SFFV_LaG17_synNotch_TetRVP64
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11269)
  AUTHORS   Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim
            WA
  TITLE     Engineering Customized Cell Sensing and Response Behaviors Using
            Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012.
            Epub 2016 Jan 28.
   PUBMED   26830878
REFERENCE   2  (bases 1 to 11269)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11269)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi:
            "10.1016/j.cell.2016.01.012"; journalName: "Cell"; date:
            "2016-02-11- 11"; volume: "164"; issue: "4"; pages: "780-91"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             85..318
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(343..361)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(651..670)
                     /label="pBRrevBam"
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"
     primer_bind     complement(884..903)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     1003..1025
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1063..1081)
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     promoter        1149..1253
                     /label="AmpR promoter"
     CDS             1254..2111
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2285..2873
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     3027..3044
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     promoter        3119..3448
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     intron          4582..4647
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4777..4797
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    5222..5356
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             5524..6157
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6204..6329
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6826..7059
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             7244..7288
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             7437..7478
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    7573..7690
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        7843..8250
                     /label="SFFV promoter"
                     /note="spleen focus-forming virus long terminal repeat
                     (LTR) promoter"
     regulatory      8318..8327
                     /label="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8387..8416
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     protein_bind    9779..9799
                     /label="attB5"
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     CDS             9800..10543
                     /label="tTA-Advanced"
                     /note="improved tetracycline-controlled transactivator"
     misc_feature    10656..11244
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(11247..11263)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(11248..11264)
                     /label="pBluescriptKS"
                     /note="For pBluescript vector"
ORIGIN
        1 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
       61 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
      121 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
      181 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
      241 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
      301 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
      361 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
      421 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
      481 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
      541 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
      601 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
      661 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
      721 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
      781 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
      841 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
      901 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      961 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     1021 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     1081 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     1141 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1201 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1261 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     1321 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     1381 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     1441 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     1501 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     1561 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     1621 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     1681 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     1741 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     1801 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     1861 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     1921 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     1981 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2041 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2101 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2161 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2221 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     2281 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     2341 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     2401 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     2461 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     2521 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     2581 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     2641 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     2701 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     2761 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     2821 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     2881 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     2941 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3001 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3061 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     3121 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     3181 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     3241 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     3301 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     3361 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     3421 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     3481 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     3541 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     3601 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     3661 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     3721 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     3781 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     3841 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     3901 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     3961 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     4021 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     4081 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     4141 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     4201 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     4261 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     4321 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     4381 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     4441 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     4501 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     4561 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     4621 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     4681 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     4741 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     4801 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     4861 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     4921 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     4981 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     5041 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     5101 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     5161 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     5221 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     5281 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     5341 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     5401 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     5461 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     5521 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     5581 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     5641 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     5701 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     5761 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     5821 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     5881 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     5941 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     6001 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     6061 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     6121 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     6181 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     6241 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     6301 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     6361 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     6421 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     6481 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     6541 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     6601 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     6661 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     6721 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     6781 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     6841 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     6901 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     6961 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     7021 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     7081 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     7141 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     7201 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     7261 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
     7321 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
     7381 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
     7441 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
     7501 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc aaatggcagt
     7561 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     7621 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     7681 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggatcg ataagcttga
     7741 tatcgaattc ctgcagcccc gataaaataa aagattttat ttagtctcca gaaaaagggg
     7801 ggaatgaaag accccacctg taggtttggc aagctagctg cagtaacgcc attttgcaag
     7861 gcatggaaaa ataccaaacc aagaatagag aagttcagat caagggcggg tacatgaaaa
     7921 tagctaacgt tgggccaaac aggatatctg cggtgagcag tttcggcccc ggcccggggc
     7981 caagaacaga tggtcaccgc agtttcggcc ccggcccgag gccaagaaca gatggtcccc
     8041 agatatggcc caaccctcag cagtttctta agacccatca gatgtttcca ggctccccca
     8101 aggacctgaa atgaccctgc gccttatttg aattaaccaa tcagcctgct tctcgcttct
     8161 gttcgcgcgc ttctgcttcc cgagctctat aaaagagctc acaacccctc actcggcgcg
     8221 ccagtcctcc gacagactga gtcgcccggg ggggatctgg agctctcgag aattctcacg
     8281 cgtcaagtgg agcaaggcag gtggacagtg gatcgccgcc accatggcat tgcccgtgac
     8341 cgccctgctg ctgccactgg ccttgttgct ccacgccgcg cggccagaac agaagctgat
     8401 cagcgaggag gatctgatgg ctgatgtgca actcgtagag tccggtggcg gactcgtcca
     8461 ggcgggaggc tccctgcgcc tgagttgcgc tgcgtccgga agaactatta gcatggccgc
     8521 catgagttgg ttcagacagg cgcctggaaa ggagagggag ttcgtggcag gcataagtcg
     8581 ctccgcaggg tcagccgtcc atgccgactc cgtaaaggga cgctttacca tctccagaga
     8641 taacacaaaa aacacgctct atctccaaat gaattctttg aaggccgagg acacagccgt
     8701 gtactactgc gccgttagga ccagcggatt ttttggaagc attcctagga cagggacagc
     8761 ctttgattac tggggccagg gaacgcaggt taccgtttcc atcctggact acagcttcac
     8821 aggtggcgct gggcgcgaca ttcccccacc gcagattgag gaggcctgtg agctgcctga
     8881 gtgccaggtg gatgcaggca ataaggtctg caacctgcag tgtaataatc acgcatgtgg
     8941 ctgggatggt ggcgactgct ccctcaactt caatgacccc tggaagaact gcacgcagtc
     9001 tctacagtgc tggaagtatt ttagcgacgg ccactgtgac agccagtgca actcggccgg
     9061 ctgcctcttt gatggcttcg actgccagct caccgaggga cagtgcaacc ccctgtatga
     9121 ccagtactgc aaggaccact tcagtgatgg ccactgcgac cagggctgta acagtgccga
     9181 atgtgagtgg gatggcctag actgtgctga gcatgtaccc gagcggctgg cagccggcac
     9241 cctggtgctg gtggtgctgc ttccacccga ccagctacgg aacaactcct tccactttct
     9301 gcgggagctc agccacgtgc tgcacaccaa cgtggtcttc aagcgtgatg cgcaaggcca
     9361 gcagatgatc ttcccgtact atggccacga ggaagagctg cgcaagcacc caatcaagcg
     9421 ctctacagtg ggttgggcca cctcttcact gcttcctggt accagtggtg ggcgccagcg
     9481 cagggagctg gaccccatgg acatccgtgg ctccattgtc tacctggaga tcgacaaccg
     9541 gcaatgtgtg cagtcatcct cgcagtgctt ccagagtgcc accgatgtgg ctgccttcct
     9601 aggtgctctt gcgtcacttg gcagcctcaa tattccttac aagattgagg ccgtgaagag
     9661 tgagccggtg gagcctccgc tgccctcgca gctgcacctc atgtacgtgg cagcggccgc
     9721 cttcgtgctc ctgttctttg tgggctgtgg ggtgctgctg tcccgcaagc gccggcggca
     9781 actttgtata caaaagttga tgtctagact ggacaagagc aaagtcataa actctgctct
     9841 ggaattactc aatgaagtcg gtatcgaagg cctgacgaca aggaaactcg ctcaaaagct
     9901 gggagttgag cagcctaccc tgtactggca cgtgaagaac aagcgggccc tgctcgatgc
     9961 cctggcaatc gagatgctgg acaggcatca tacccacttc tgccccctgg aaggcgagtc
    10021 atggcaagac tttctgcgga acaacgccaa gtcattccgc tgtgctctcc tctcacatcg
    10081 cgacggggct aaagtgcatc tcggcacccg cccaacagag aaacagtacg aaaccctgga
    10141 aaatcagctc gcgttcctgt gtcagcaagg cttctccctg gagaacgcac tgtacgctct
    10201 gtccgccgtg ggccacttta cactgggctg cgtattggag gatcaggagc atcaagtagc
    10261 aaaagaggaa agagagacac ctaccaccga ttctatgccc ccacttctga gacaagcaat
    10321 tgagctgttc gaccatcagg gagccgaacc tgccttcctt ttcggcctgg aactaatcat
    10381 atgtggcctg gagaaacagc taaagtgcga aagcggcggg ccggccgacg cccttgacga
    10441 ttttgactta gacatgctcc cagccgatgc ccttgacgac tttgaccttg atatgctgcc
    10501 tgctgacgct cttgacgatt ttgaccttga catgctcccc gggtaagatc cttgacttgc
    10561 ggccgcaact cccacctgca acatgcgtga ctgactgagg ccgcgactct agagtcgacc
    10621 tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt
    10681 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
    10741 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
    10801 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
    10861 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
    10921 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
    10981 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
    11041 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
    11101 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
    11161 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
    11221 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaa
//
