LOCUS       V010596                11423 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V010596
VERSION     V010596
KEYWORDS    pHR_PGK_antiHer24D5-7_synNotch_Gal4VP64
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11423)
  AUTHORS   Roybal KT, Williams JZ, Morsut L, Rupp LJ, Kolinko I, Choe JH,
            Walker WJ, McNally KA, Lim WA
  TITLE     Engineering T Cells with Customized Therapeutic Response Programs
            Using Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Oct 6;167(2):419-432.e16. doi:
            10.1016/j.cell.2016.09.011. Epub 2016 Sep 29.
   PUBMED   27693353
REFERENCE   2  (bases 1 to 11423)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11423)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi:
            "10.1016/j.cell.2016.09.011"; journalName: "Cell"; date: "2016-10-6-
            6"; volume: "167"; issue: "2"; pages: "419-432.e16"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             64..93
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             1810..2250
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             2287..2436
                     /label="VP64"
                     /note="tetrameric repeat of the minimal activation domain
                     of herpes simplex virus VP16 (Beerli et al., 1998)"
     misc_feature    2555..3141
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             3206..3439
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3464..3482)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3772..3791)
                     /label="pBRrevBam"
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"
     primer_bind     complement(4005..4024)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     4124..4146
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4184..4202)
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     promoter        4270..4374
                     /label="AmpR promoter"
     CDS             4375..5232
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5406..5994
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     6148..6165
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     promoter        6240..6569
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     intron          7703..7768
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7898..7918
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    8343..8477
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             8645..9278
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9325..9450
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    9947..10180
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             10365..10409
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             10558..10599
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    10694..10811
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        10871..11370
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
ORIGIN
        1 atggccctgc ctgttaccgc cctgttgctc cctcttgccc tgctgcttca cgccgctcgc
       61 cctgagcaga aacttatatc agaggaagat ctggatattc agatgactca gtctcctagc
      121 tctctctcag ctagtgtcgg ggacagagtg acaataacct gcagagcctc acaggacgtg
      181 aatactgcgg tcgcctggta tcagcagaaa cctgggaagg cacccaaact gctcatttac
      241 tccgctagct tcttggagag cggcgtccca tcacggttct ccgggagtcg cagcggcacc
      301 gacttcaccc ttaccatcag ctctttgcag ccagaggatt ttgcaacgta ttattgccaa
      361 cagcactaca ctactccccc aactttcgga caaggcacaa aagttgaaat taaaaggact
      421 ggaagtacca gcggatccgg gaaaccgggt tccggtgaag gcagtgaggt gcaactggtt
      481 gagagcggag gcggtctggt gcagcctggt ggttctctcc gcctcagctg cgctgcttcc
      541 ggtttcaaca taaaagacac ttatattcac tgggtcaggc aggcaccagg caagggactc
      601 gagtgggttg cgcgcattta tccgactaac gggtacacaa ggtacgctga tagtgtgaag
      661 ggtagattta cgattagcgc cgacacctcc aaaaatacag catatctcca gatgaatagc
      721 cttcgcgctg aagacacagc tgtatactac tgcagtagat ggggcggtga tggcttctac
      781 gccatggacg tgtggggtca gggaactctg gtgaccgttt caagcggtag tatcctggac
      841 tacagcttca caggtggcgc tgggcgcgac attcccccac cgcagattga ggaggcctgt
      901 gagctgcctg agtgccaggt ggatgcaggc aataaggtct gcaacctgca gtgtaataat
      961 cacgcatgtg gctgggatgg tggcgactgc tccctcaact tcaatgaccc ctggaagaac
     1021 tgcacgcagt ctctacagtg ctggaagtat tttagcgacg gccactgtga cagccagtgc
     1081 aactcggccg gctgcctctt tgatggcttc gactgccagc tcaccgaggg acagtgcaac
     1141 cccctgtatg accagtactg caaggaccac ttcagtgatg gccactgcga ccagggctgt
     1201 aacagtgccg aatgtgagtg ggatggccta gactgtgctg agcatgtacc cgagcggctg
     1261 gcagccggca ccctggtgct ggtggtgctg cttccacccg accagctacg gaacaactcc
     1321 ttccactttc tgcgggagct cagccacgtg ctgcacacca acgtggtctt caagcgtgat
     1381 gcgcaaggcc agcagatgat cttcccgtac tatggccacg aggaagagct gcgcaagcac
     1441 ccaatcaagc gctctacagt gggttgggcc acctcttcac tgcttcctgg taccagtggt
     1501 gggcgccagc gcagggagct ggaccccatg gacatccgtg gctccattgt ctacctggag
     1561 atcgacaacc ggcaatgtgt gcagtcatcc tcgcagtgct tccagagtgc caccgatgtg
     1621 gctgccttcc taggtgctct tgcgtcactt ggcagcctca atattcctta caagattgag
     1681 gccgtgaaga gtgagccggt ggagcctccg ctgccctcgc agctgcacct catgtacgtg
     1741 gcagcggccg ccttcgtgct cctgttcttt gtgggctgtg gggtgctgct gtcccgcaag
     1801 cgccggcgga tgaagctgct gagcagcatc gagcaggcct gtgacatctg ccggctgaag
     1861 aaactgaagt gcagcaaaga aaagcccaag tgcgccaagt gcctgaagaa caactgggag
     1921 tgccggtaca gccccaagac caagagaagc cccctgacca gagcccacct gaccgaggtg
     1981 gaaagccggc tggaaagact ggaacagctg tttctgctga tcttcccacg cgaggacctg
     2041 gacatgatcc tgaagatgga cagcctgcag gacatcaagg ccctgctgac cggcctgttc
     2101 gtgcaggaca acgtgaacaa ggacgccgtg accgacagac tggccagcgt ggaaaccgac
     2161 atgcccctga ccctgcggca gcacagaatc agcgccacca gcagcagcga ggaaagcagc
     2221 aacaagggcc agcggcagct gacagtgtct gctgctgcag gcggaagcgg aggctctggc
     2281 ggatctgatg ccctggacga cttcgacctg gatatgctgg gcagcgacgc cctggatgat
     2341 tttgatctgg acatgctggg atctgacgct ctggacgatt tcgatctcga catgttggga
     2401 tcagatgcac tggatgactt tgacctggac atgctcggat catgagatcc ttgacttgcg
     2461 gccgcaactc ccacctgcaa catgcgtgac tgactgaggc cgcgactcta gagtcgacct
     2521 gcaggcatgc aagcttgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     2581 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     2641 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     2701 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2761 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2821 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2881 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2941 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     3001 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     3061 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     3121 ctccctttgg gccgcctccc cttaagacca atgacttaca aggcagctgt agatcttagc
     3181 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     3241 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct
     3301 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta
     3361 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca
     3421 gtgtggaaaa tctctagcag catctagaat taattccgtg tattctatag tgtcacctaa
     3481 atcgtatgtg tatgatacat aaggttatgt attaattgta gccgcgttct aacgacaata
     3541 tgtacaagcc taattgtgta gcatctggct tactgaagca gaccctatca tctctctcgt
     3601 aaactgccgt cagagtcggt ttggttggac gaaccttctg agtttctggt aacgccgtcc
     3661 cgcacccgga aatggtcagc gaaccaatca gcagggtcat cgctagccag atcctctacg
     3721 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg
     3781 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg
     3841 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg
     3901 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa
     3961 tgcaggagtc gcataaggga gagcgtcgaa tggtgcactc tcagtacaat ctgctctgat
     4021 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
     4081 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
     4141 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
     4201 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
     4261 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4321 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4381 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4441 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4501 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     4561 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     4621 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     4681 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     4741 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     4801 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     4861 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     4921 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     4981 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5041 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5101 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5161 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5221 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5281 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5341 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5401 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5461 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     5521 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     5581 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     5641 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     5701 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     5761 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     5821 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     5881 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     5941 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6001 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6061 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6121 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6181 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctgtggaatg
     6241 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6301 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6361 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6421 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6481 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     6541 gcttttttgg aggcctaggc ttttgcaaaa agcttggaca caagacaggc ttgcgagata
     6601 tgtttgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac
     6661 tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca acctattttc
     6721 ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat gagcgaaaaa
     6781 tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag ccgactgatg
     6841 ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctgta ccgggtgcgt
     6901 tactggcgcg tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc
     6961 acgacaacca gcgcgagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca
     7021 tcgttattga tgacctggtg gataccggtg gtactgcggt tgcgattcgt gaaatgtatc
     7081 caaaagcgca ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact
     7141 atgttgttga tatcccgcaa gatacctgga ttgaacagcc gtgggatatg ggcgtcgtat
     7201 tcgtcccgcc aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc
     7261 tttttaactt caggcgggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg
     7321 acacaggcaa acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg
     7381 acgctagtcc gccgctttaa tcacggcgca caaccgcctg tgcagtcggc ccttgatggt
     7441 aaaaccatcc ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggcatt
     7501 gacccacgcg aaatcctcga cgtccaggca cgtattgtga tgagcgatgc cgaacgtacc
     7561 gacgatgatt tatacgatac ggtgattggc taccgtggcg gcaactggat ttatgagtgg
     7621 gccccggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc
     7681 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact
     7741 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca
     7801 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga
     7861 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga
     7921 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa
     7981 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa
     8041 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa
     8101 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca
     8161 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta
     8221 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     8281 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     8341 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     8401 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     8461 cttatcatgt ctggatcaac tggataactc aagctaacca aaatcatccc aaacttccca
     8521 ccccataccc tattaccact gccaattacc tgtggtttca tttactctaa acctgtgatt
     8581 cctctgaatt attttcattt taaagaaatt gtatttgtta aatatgtact acaaacttag
     8641 tagttggaag ggctaattca ctcccaaaga agacaagata tccttgatct gtggatctac
     8701 cacacacaag gctacttccc tgattagcag aactacacac cagggccagg ggtcagatat
     8761 ccactgacct ttggatggtg ctacaagcta gtaccagttg agccagataa ggtagaagag
     8821 gccaataaag gagagaacac cagcttgtta caccctgtga gcctgcatgg gatggatgac
     8881 ccggagagag aagtgttaga gtggaggttt gacagccgcc tagcatttca tcacgtggcc
     8941 cgagagctgc atccggagta cttcaagaac tgctgatatc gagcttgcta caagggactt
     9001 tccgctgggg actttccagg gaggcgtggc ctgggcggga ctggggagtg gcgagccctc
     9061 agatcctgca tataagcagc tgctttttgc ctgtactggg tctctctggt tagaccagat
     9121 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     9181 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9241 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     9301 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     9361 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     9421 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     9481 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     9541 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9601 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9661 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     9721 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9781 cagcaagcgg ccggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga
     9841 gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca
     9901 aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc
     9961 ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac
    10021 aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg
    10081 aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa
    10141 tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg
    10201 gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg
    10261 aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa
    10321 gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat
    10381 tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt
    10441 ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg
    10501 ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc
    10561 acctcccaac cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga
    10621 gagacagaga cagatccatt cgattagtga acggatctcg acggtatcgc caaatggcag
    10681 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
    10741 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
    10801 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggatc gataagcttg
    10861 atatcgaatt gggtagggga ggcgcttttc ccaaggcagt ctggagcatg cgctttagca
    10921 gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca ttccacatcc
    10981 accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc ttctactcct
    11041 cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg
    11101 gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca atggaagcgg
    11161 gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg ggctcagagg
    11221 ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg ggcgggcgcc
    11281 cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg
    11341 ttctcctctt cctcatctcc gggcctttcg aattctcacg cgtcaagtgg agcaaggcag
    11401 gtggacagtg gatcgccgcc acc
//
