LOCUS       V010552                11449 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V010552
VERSION     V010552
KEYWORDS    pHR_PGK_antiCD19_synNotch_Gal4VP64
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11449)
  AUTHORS   Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim
            WA
  TITLE     Engineering Customized Cell Sensing and Response Behaviors Using
            Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012.
            Epub 2016 Jan 28.
   PUBMED   26830878
REFERENCE   2  (bases 1 to 11449)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11449)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi:
            "10.1016/j.cell.2016.01.012"; journalName: "Cell"; date:
            "2016-02-11- 11"; volume: "164"; issue: "4"; pages: "780-91"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11449
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     276..295
                     /label="mPGK-F"
                     /note="Mouse PGK promoter, forward primer"
     CDS             451..480
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label="Myc"
                     /translation="EQKLISEEDL"
     CDS             2185..2625
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             2662..2811
                     /label="VP64"
                     /note="tetrameric repeat of the minimal activation domain
                     of herpes simplex virus VP16 (Beerli et al., 1998)"
     misc_feature    2930..3518
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(3521..3537)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(3522..3538)
                     /label="pBluescriptKS"
                     /note="For pBluescript vector"
     LTR             3628..3861
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3886..3904)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4194..4213)
                     /label="pBRrevBam"
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"
     primer_bind     complement(4427..4446)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     4546..4568
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4606..4624)
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     promoter        4692..4796
                     /label="AmpR promoter"
     CDS             4797..5654
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      5828..6416
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     6570..6587
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     promoter        6662..6991
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     intron          8125..8190
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8320..8340
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    8765..8899
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             9067..9700
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9747..9872
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    10369..10602
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             10787..10831
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             10980..11021
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    11116..11233
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     primer_bind     complement(11328..11351)
                     /label="MSCV-rev"
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag
       61 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga
      121 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt
      181 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg
      241 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac
      301 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgaattctc acgcgtcaag
      361 tggagcaagg caggtggaca gtggatcatg gcgctccctg tcaccgcact gcttcttccg
      421 ctggcactgc tgctgcacgc tgcacggcct gagcaaaaac ttatctctga agaggacctc
      481 gatatacaga tgacgcagac aacgtcaagt ctttccgcca gcttgggaga ccgagtgact
      541 atatcttgta gagcaagcca ggatatttct aagtatctta actggtacca acaaaagccc
      601 gatggaacgg ttaagctgct tatataccat accagtagac tccactccgg cgtaccatca
      661 cggttttctg gcagtggctc cgggaccgac tattctttga cgatctctaa tctcgaacaa
      721 gaggatattg caacatactt ttgtcagcaa ggcaatacct tgccatatac gtttgggggc
      781 gggacaaaac ttgagataac cggcggcggt ggttcaggcg gtggcggttc cggtggtggg
      841 ggatcagagg ttaagcttca ggaatccgga ccaggtttgg ttgcccccag ccaatctctc
      901 agcgttacat gcacggtttc aggcgtcagt ctccccgatt acggtgtaag ttggattcgg
      961 caacctccgc gaaagggtct ggaatggctg ggggttattt gggggagtga gacaacttat
     1021 tacaactctg cacttaagag tcggcttacc atcatcaagg ataattcaaa atcacaagta
     1081 ttcctgaaga tgaactcatt gcaaacagat gatacagcta tatactattg tgccaagcat
     1141 tactattatg gtggttctta tgcaatggat tactgggggc aaggcacgtc agtgacagtg
     1201 agttcaatcc tggactacag cttcacaggt ggcgctgggc gcgacattcc cccaccgcag
     1261 attgaggagg cctgtgagct gcctgagtgc caggtggatg caggcaataa ggtctgcaac
     1321 ctgcagtgta ataatcacgc atgtggctgg gatggtggcg actgctccct caacttcaat
     1381 gacccctgga agaactgcac gcagtctcta cagtgctgga agtattttag cgacggccac
     1441 tgtgacagcc agtgcaactc ggccggctgc ctctttgatg gcttcgactg ccagctcacc
     1501 gagggacagt gcaaccccct gtatgaccag tactgcaagg accacttcag tgatggccac
     1561 tgcgaccagg gctgtaacag tgccgaatgt gagtgggatg gcctagactg tgctgagcat
     1621 gtacccgagc ggctggcagc cggcaccctg gtgctggtgg tgctgcttcc acccgaccag
     1681 ctacggaaca actccttcca ctttctgcgg gagctcagcc acgtgctgca caccaacgtg
     1741 gtcttcaagc gtgatgcgca aggccagcag atgatcttcc cgtactatgg ccacgaggaa
     1801 gagctgcgca agcacccaat caagcgctct acagtgggtt gggccacctc ttcactgctt
     1861 cctggtacca gtggtgggcg ccagcgcagg gagctggacc ccatggacat ccgtggctcc
     1921 attgtctacc tggagatcga caaccggcaa tgtgtgcagt catcctcgca gtgcttccag
     1981 agtgccaccg atgtggctgc cttcctaggt gctcttgcgt cacttggcag cctcaatatt
     2041 ccttacaaga ttgaggccgt gaagagtgag ccggtggagc ctccgctgcc ctcgcagctg
     2101 cacctcatgt acgtggcagc ggccgccttc gtgctcctgt tctttgtggg ctgtggggtg
     2161 ctgctgtccc gcaagcgccg gcggatgaag ctgctgagca gcatcgagca ggcctgtgac
     2221 atctgccggc tgaagaaact gaagtgcagc aaagaaaagc ccaagtgcgc caagtgcctg
     2281 aagaacaact gggagtgccg gtacagcccc aagaccaaga gaagccccct gaccagagcc
     2341 cacctgaccg aggtggaaag ccggctggaa agactggaac agctgtttct gctgatcttc
     2401 ccacgcgagg acctggacat gatcctgaag atggacagcc tgcaggacat caaggccctg
     2461 ctgaccggcc tgttcgtgca ggacaacgtg aacaaggacg ccgtgaccga cagactggcc
     2521 agcgtggaaa ccgacatgcc cctgaccctg cggcagcaca gaatcagcgc caccagcagc
     2581 agcgaggaaa gcagcaacaa gggccagcgg cagctgacag tgtctgctgc tgcaggcgga
     2641 agcggaggct ctggcggatc tgatgccctg gacgacttcg acctggatat gctgggcagc
     2701 gacgccctgg atgattttga tctggacatg ctgggatctg acgctctgga cgatttcgat
     2761 ctcgacatgt tgggatcaga tgcactggat gactttgacc tggacatgct cggatcatga
     2821 gatccttgac ttgcggccgc aactcccacc tgcaacatgc gtgactgact gaggccgcga
     2881 ctctagagtc gacctgcagg catgcaagct tgatatcaag cttatcgata atcaacctct
     2941 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
     3001 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
     3061 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
     3121 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
     3181 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
     3241 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
     3301 caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc
     3361 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
     3421 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
     3481 tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc gacctcgagg
     3541 gaattaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
     3601 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     3661 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     3721 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     3781 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     3841 cagtgtggaa aatctctagc agcatctaga attaattccg tgtattctat agtgtcacct
     3901 aaatcgtatg tgtatgatac ataaggttat gtattaattg tagccgcgtt ctaacgacaa
     3961 tatgtacaag cctaattgtg tagcatctgg cttactgaag cagaccctat catctctctc
     4021 gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc tgagtttctg gtaacgccgt
     4081 cccgcacccg gaaatggtca gcgaaccaat cagcagggtc atcgctagcc agatcctcta
     4141 cgccggacgc atcgtggccg gcatcaccgg cgccacaggt gcggttgctg gcgcctatat
     4201 cgccgacatc accgatgggg aagatcgggc tcgccacttc gggctcatga gcgcttgttt
     4261 cggcgtgggt atggtggcag gccccgtggc cgggggactg ttgggcgcca tctccttgca
     4321 tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac ctactactgg gctgcttcct
     4381 aatgcaggag tcgcataagg gagagcgtcg aatggtgcac tctcagtaca atctgctctg
     4441 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     4501 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt
     4561 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc
     4621 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     4681 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4741 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4801 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4861 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4921 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4981 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5041 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5101 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5161 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5221 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5281 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5341 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5401 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5461 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5521 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5581 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5641 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5701 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5761 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5821 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5881 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5941 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6001 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6061 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6121 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6181 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6241 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6301 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6361 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6421 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6481 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6541 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6601 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgtggaa
     6661 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     6721 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     6781 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     6841 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6901 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     6961 aggctttttt ggaggcctag gcttttgcaa aaagcttgga cacaagacag gcttgcgaga
     7021 tatgtttgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa aaagacgcgg
     7081 actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg caacctattt
     7141 tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac atgagcgaaa
     7201 aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca agccgactga
     7261 tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg taccgggtgc
     7321 gttactggcg cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga
     7381 tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca gaaggcgatg gcgaaggctt
     7441 catcgttatt gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta
     7501 tccaaaagcg cactttgtca ccatcttcgc aaaaccggct ggtcgtccgc tggttgatga
     7561 ctatgttgtt gatatcccgc aagatacctg gattgaacag ccgtgggata tgggcgtcgt
     7621 attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt
     7681 tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca
     7741 tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct
     7801 cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gcccttgatg
     7861 gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggca
     7921 ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat gccgaacgta
     7981 ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg atttatgagt
     8041 gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt ggacaaacta
     8101 cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata atgtgttaaa
     8161 ctactgattc taattgtttg tgtattttag attccaacct atggaactga tgaatgggag
     8221 cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat gccatctagt
     8281 gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa gagaaaggta
     8341 gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt
     8401 aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc actgctatac
     8461 aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag ttataatcat
     8521 aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa taactatgct
     8581 caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga atatttgatg
     8641 tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     8701 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     8761 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8821 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8881 atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc
     8941 caccccatac cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga
     9001 ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt
     9061 agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct
     9121 accacacaca aggctacttc cctgattagc agaactacac accagggcca ggggtcagat
     9181 atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag
     9241 aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg
     9301 acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg
     9361 cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac
     9421 tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc
     9481 tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag
     9541 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     9601 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     9661 tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact
     9721 tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc
     9781 gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag
     9841 gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc
     9901 gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag
     9961 tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag
    10021 aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac
    10081 ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa
    10141 aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg
    10201 cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg
    10261 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
    10321 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
    10381 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
    10441 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
    10501 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
    10561 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
    10621 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
    10681 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
    10741 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
    10801 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
    10861 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
    10921 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
    10981 ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag aaggtggaga
    11041 gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc gccaaatggc
    11101 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
    11161 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
    11221 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga tcgataagct
    11281 tgatatcgaa ttgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
    11341 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
    11401 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcc
//
