LOCUS       Exported               11280 bp DNA     circular SYN 29-JUL-2025
DEFINITION  Exported.
ACCESSION   V006799
VERSION     .
KEYWORDS    pHR_Gal4UAS_IRES_mC_pGK_tBFP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11280)
  AUTHORS   Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim 
            WA
  TITLE     Engineering Customized Cell Sensing and Response Behaviors Using 
            Synthetic Notch Receptors.
  JOURNAL   Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. 
            Epub 2016 Jan 28.
  PUBMED    26830878
REFERENCE   2  (bases 1 to 11280)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 11280)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11280)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2016.01.012"; journalName: "Cell"; date: 
            "2016-02-11- 11"; volume: "164"; issue: "4"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          1..574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     source          575..11280
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..574
                     /label=IRES
                     /note="IRES"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (ECMV)"
     CDS             593..1300
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     promoter        1388..1875
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             1896..2594
                     /label=TagBFP
                     /note="monomeric blue fluorescent protein"
     misc_feature    2633..3221
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(3224..3240)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3225..3241)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3331..3564
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3589..3607)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3897..3916)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"
     primer_bind     complement(4130..4149)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     4249..4271
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4309..4327)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     promoter        4395..4499
                     /label=AmpR promoter
     CDS             4500..5357
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      5531..6119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6273..6290
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        6365..6694
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          7828..7893
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8023..8043
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    8468..8602
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             8770..9403
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9450..9575
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    10072..10305
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10490..10534
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             10683..10724
                     /label=Protein Tat
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    10819..10936
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     misc_feature    10997..11082
                     /label=Gal4
                     /note="Gal4"
     promoter        11129..11246
                     /note="minimal CMV promoter"
ORIGIN
        1 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
       61 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
      121 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
      181 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
      241 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
      301 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
      361 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
      421 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
      481 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
      541 ggacgtggtt ttcctttgaa aaacacgatg ataaatgagc gctggcgggt ccatggtgag
      601 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
      661 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
      721 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
      781 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
      841 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
      901 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
      961 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     1021 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     1081 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     1141 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     1201 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     1261 gggccgccac tccaccggcg gcatggacga gctgtacaag tagggccgca actcccacct
     1321 gcaacatgcg tgactgactg aggccgcgac tctagagtcg acctgcacga ggttaacgaa
     1381 ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     1441 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     1501 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     1561 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     1621 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     1681 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     1741 ggaaggggtg ggtccggggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     1801 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     1861 tctccgggcc tttcgacctg cagcccaagc ttaccatgag cgagctgatt aaggagaaca
     1921 tgcacatgaa gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg
     1981 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
     2041 gccctctccc cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct
     2101 tcatcaacca cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca
     2161 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     2221 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca
     2281 acggccctgt gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc
     2341 ccgctgacgg cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc
     2401 atctgatcgc aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga
     2461 tgcctggcgt ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga
     2521 cctacgtcga gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg
     2581 ggcacaagct taattaatgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc
     2641 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
     2701 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
     2761 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
     2821 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
     2881 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
     2941 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     3001 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
     3061 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
     3121 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
     3181 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
     3241 agggaattaa ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     3301 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     3361 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3421 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3481 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3541 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
     3601 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
     3661 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
     3721 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
     3781 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
     3841 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
     3901 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
     3961 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
     4021 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
     4081 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
     4141 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     4201 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     4261 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     4321 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     4381 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     4441 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     4501 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     4561 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     4621 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     4681 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     4741 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     4801 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     4861 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     4921 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     4981 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     5041 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     5101 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     5161 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     5221 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     5281 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     5341 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     5401 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     5461 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     5521 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     5581 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     5641 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     5701 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     5761 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     5821 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     5881 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     5941 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     6001 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     6061 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     6121 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     6181 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     6241 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     6301 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
     6361 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     6421 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     6481 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     6541 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6601 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6661 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
     6721 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
     6781 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
     6841 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
     6901 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
     6961 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
     7021 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
     7081 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
     7141 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
     7201 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
     7261 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
     7321 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
     7381 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
     7441 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
     7501 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
     7561 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
     7621 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
     7681 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
     7741 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
     7801 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
     7861 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
     7921 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
     7981 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
     8041 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
     8101 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
     8161 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
     8221 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
     8281 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
     8341 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
     8401 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     8461 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     8521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     8581 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
     8641 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
     8701 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
     8761 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
     8821 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
     8881 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
     8941 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
     9001 atgacccgta gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
     9061 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
     9121 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
     9181 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
     9241 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     9301 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     9361 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     9421 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     9481 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     9541 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     9601 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     9661 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     9721 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     9781 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     9841 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     9901 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     9961 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
    10021 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
    10081 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
    10141 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
    10201 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
    10261 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
    10321 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
    10381 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
    10441 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
    10501 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
    10561 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
    10621 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
    10681 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
    10741 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat
    10801 ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
    10861 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
    10921 aaaaattcaa aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa
    10981 gcttgatatc gaattcggag cactgtcctc cgaacgtcgg agcactgtcc tccgaacgtc
    11041 ggagcactgt cctccgaacg tcggagcact gtcctccgaa cggagcatgt cctccgaacg
    11101 tcggagcact gtcctccgaa cgactagtta ggcgtgtacg gtgggaggcc tatataagca
    11161 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
    11221 atagaagaca ccgggaccga tccagcctct cgacattcgt tggatccttg acttgcggcc
//
