LOCUS       V014799                11217 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014799
VERSION     V014799
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11217)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11217
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          455..520
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             650..670
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    1095..1229
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             1397..2030
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2077..2202
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    2699..2932
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             3117..3161
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             3310..3351
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    3446..3563
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             3894..4610
                     /label="EGFP"
                     /note="enhanced GFP"
     promoter        4736..5235
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5256..5963
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             6027..6398
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     misc_feature    6477..7065
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             7175..7408
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(7433..7451)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        8239..8343
                     /label="AmpR promoter"
     CDS             8344..9201
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      9375..9963
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        10209..10538
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
ORIGIN
        1 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
       61 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
      121 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
      181 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
      241 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
      301 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
      361 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
      421 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
      481 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
      541 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
      601 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
      661 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
      721 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
      781 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
      841 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
      901 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
      961 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     1021 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     1081 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     1201 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     1261 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     1321 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     1381 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     1441 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     1501 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     1561 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     1621 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     1681 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     1741 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     1801 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     1861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     2041 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     2101 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     2161 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     2221 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     2281 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     2341 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     2401 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     2461 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     2521 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     2581 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     2641 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     2701 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     2761 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     2821 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     2881 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     2941 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     3001 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     3061 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     3121 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     3181 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     3241 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     3301 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     3361 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     3421 gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     3481 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3541 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
     3601 tcgataagct tgatatcgaa ttcggagcac tgtcctccga acgtcggagc actgtcctcc
     3661 gaacgtcgga gcactgtcct ccgaacgtcg gagcactgtc ctccgaacgg agcatgtcct
     3721 ccgaacgtcg gagcactgtc ctccgaacga ctagttaggc gtgtacggtg ggaggcctat
     3781 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     3841 gacctccata gaagacaccg ggaccgatcc agcctctcga cattcgttgg atcatggtga
     3901 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3961 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4021 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4081 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     4141 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4201 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4261 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4321 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4381 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4441 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4501 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4561 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taagatcctt
     4621 gacttgcggc cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga
     4681 gtcgacctgc atctaggcgc cggaattaga tctctcgagg ttaacgaatt ctaccgggta
     4741 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     4801 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     4861 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     4921 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     4981 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     5041 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     5101 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     5161 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     5221 tctccgggcc tttcgacctg cagcccaagc ttaccatggt gagcaagggc gaggaggata
     5281 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     5341 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     5401 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     5461 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     5521 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     5581 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     5641 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     5701 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     5761 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     5821 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     5881 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     5941 gcggcatgga cgagctgtac aagggatctc gcgagggcag aggaagtctt ctaacatgcg
     6001 gtgacgtgga ggagaatccc ggccctatgg ccaagttgac cagtgccgtt ccggtgctca
     6061 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     6121 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     6181 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     6241 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     6301 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     6361 ccggcaactg cgtgcacttc gtggccgagg agcaggactg agcggccgcg actctagagt
     6421 cgacctgcag gcatgcaagc ttgcaggcat gcaagcttga tatcaagctt atcgataatc
     6481 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6541 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6601 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6661 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6721 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6781 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     6841 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     6901 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     6961 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7021 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     7081 ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta
     7141 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7201 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     7261 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7321 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     7381 ttttagtcag tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt
     7441 gtcacctaaa tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta
     7501 acgacaatat gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat
     7561 ctctctcgta aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta
     7621 acgccgtccc gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga
     7681 tcctctacgc cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg
     7741 cctatatcgc cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg
     7801 cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct
     7861 ccttgcatgc accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct
     7921 gcttcctaat gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc
     7981 tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     8041 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc
     8101 tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg
     8161 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     8221 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8281 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8341 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     8401 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     8461 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     8521 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8581 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8641 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8701 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8761 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8821 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8881 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8941 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     9001 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     9061 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     9121 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     9181 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     9241 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9301 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9361 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     9421 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     9481 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     9541 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     9601 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     9661 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     9721 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     9781 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     9841 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     9901 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     9961 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    10021 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    10081 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    10141 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    10201 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
    10261 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
    10321 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
    10381 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    10441 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    10501 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct
    10561 tgcgagatat gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa
    10621 gacgcggact catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa
    10681 cctattttcc cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg
    10741 agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc
    10801 cgactgatgc cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac
    10861 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
    10921 gctacgatca cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg
    10981 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
    11041 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
    11101 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
    11161 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgc
//
