LOCUS       V014761                10783 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014761
VERSION     V014761
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10783)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          455..520
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             650..670
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    1095..1229
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             1397..2030
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2077..2202
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    2699..2932
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             3117..3161
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             3310..3351
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    3446..3563
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             3894..4610
                     /label="EGFP"
                     /note="enhanced GFP"
     promoter        4736..5235
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5256..5963
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    6043..6631
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6741..6974
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(6999..7017)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        7805..7909
                     /label="AmpR promoter"
     CDS             7910..8767
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      8941..9529
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag tattctggag
       61 gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat
      121 cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg
      181 gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct gatgtggatc
      241 tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt gatgagcgat
      301 gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg cggcaactgg
      361 atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg tgacataatt
      421 ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt taagtgtata
      481 atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct atggaactga
      541 tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct cagaagaaat
      601 gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc caaaaaagaa
      661 gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt tgagtcatgc
      721 tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg aaaaagctgc
      781 actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta ggcataacag
      841 ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt ctgctattaa
      901 taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg ttaataagga
      961 atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca tttgtagagg
     1021 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
     1081 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1141 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     1201 tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac caaaatcatc
     1261 ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt catttactct
     1321 aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt taaatatgta
     1381 ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga tatccttgat
     1441 ctgtggatct accacacaca aggctacttc cctgattagc agaactacac accagggcca
     1501 ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt tgagccagat
     1561 aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt gagcctgcat
     1621 gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg cctagcattt
     1681 catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata tcgagcttgc
     1741 tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg gactggggag
     1801 tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg ggtctctctg
     1861 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     1921 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     1981 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     2041 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     2101 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     2161 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     2221 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     2281 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     2341 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     2401 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     2461 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     2521 aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga ggagatatga
     2581 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     2641 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     2701 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     2761 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     2821 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     2881 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     2941 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     3001 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     3061 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     3121 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     3181 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     3241 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     3301 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     3361 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     3421 gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
     3481 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
     3541 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga
     3601 tcgataagct tgatatcgaa ttcggagcac tgtcctccga acgtcggagc actgtcctcc
     3661 gaacgtcgga gcactgtcct ccgaacgtcg gagcactgtc ctccgaacgg agcatgtcct
     3721 ccgaacgtcg gagcactgtc ctccgaacga ctagttaggc gtgtacggtg ggaggcctat
     3781 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
     3841 gacctccata gaagacaccg ggaccgatcc agcctctcga cattcgttgg atcatggtga
     3901 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3961 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     4021 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     4081 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     4141 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     4201 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     4261 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     4321 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     4381 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4441 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4501 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4561 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taagatcctt
     4621 gacttgcggc cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga
     4681 gtcgacctgc atctaggcgc cggaattaga tctctcgagg ttaacgaatt ctaccgggta
     4741 ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact
     4801 tggcgctaca caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa
     4861 ccggctccgt tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag
     4921 ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc
     4981 actagtctcg tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc
     5041 agcggccaat agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg
     5101 tccgggggcg ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga
     5161 ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca
     5221 tctccgggcc tttcgacctg cagcccaagc ttaccatggt gagcaagggc gaggaggata
     5281 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
     5341 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
     5401 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
     5461 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
     5521 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
     5581 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     5641 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
     5701 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
     5761 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
     5821 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
     5881 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     5941 gcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagagtcgac ctgcaggcat
     6001 gcaagcttgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac
     6061 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     6121 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     6181 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     6241 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     6301 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     6361 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     6421 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     6481 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     6541 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     6601 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa
     6661 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     6721 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     6781 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     6841 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     6901 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     6961 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     7021 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     7081 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     7141 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     7201 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     7261 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     7321 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     7381 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     7441 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     7501 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc
     7561 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     7621 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     7681 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     7741 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     7801 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7861 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7921 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7981 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     8041 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8101 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8161 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8221 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8281 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8341 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8401 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8461 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8521 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8581 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8641 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8701 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8761 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8821 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8881 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8941 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     9001 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9061 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9121 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9181 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     9241 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     9301 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     9361 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     9421 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     9481 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     9541 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     9601 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     9661 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     9721 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
     9781 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     9841 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     9901 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     9961 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
    10021 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
    10081 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
    10141 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
    10201 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
    10261 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
    10321 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
    10381 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
    10441 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
    10501 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
    10561 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
    10621 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
    10681 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
    10741 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgc
//
