LOCUS       Exported               11494 bp DNA     circular SYN 12-SEP-2024
DEFINITION  S22409110002-PHRW40_for.ref.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11494)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..271
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             1611..1622
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2268..2765
                     /codon_start=1
                     /product="13 tandem Myc epitope tags"
                     /label=13xMyc
                     /translation="EQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLIS
                     EEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLD
                     GEQKLISEEDLNGEQKLISEEDLNGEQKLISEEDLNGLDGEQKLISEEDLNGEQKLISE
                     EDL"
     terminator      3075..3322
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(3345..3361)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3369..3385
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3393..3423)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3438..3459
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3747..4335)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4506..5366)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(5367..5471)
                     /gene="bla"
                     /label=AmpR promoter
     gene            5679..7035
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin 
                     resistance (Wach et al., 1994)"
     promoter        5679..6022
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
     CDS             6023..6832
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     terminator      6838..7035
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     rep_origin      7378..8215
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     misc_feature    8351..8643
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     primer_bind     9400..9416
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(9444..10451)
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     promoter        complement(10557..10760)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        10761..11140
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcg gtaccctatg
      301 gcatgcatgt gctctgtatg tatataaaac tcttgttttc ttcttttctc taaatattct
      361 ttccttatac attaggtcct ttgtagcata aattactata cttctataga cacgcaaaca
      421 caaatacaca cactaaatta ccggatcaat tcgggggaaa aatgctttct gaaaacacga
      481 ctattctgat ggctaacggt gaaattaaag acatcgcaaa cgtcacggct aactcttacg
      541 ttatgtgcgc agatggctcc gctgcccgcg tcataaatgt cacacagggc tatcagaaaa
      601 tctataatat acagcaaaaa accaaacaca gagcttttga aggtgaacct ggtaggttag
      661 atcccaggcg tagaacagtt tatcagcgtc ttgcattaca atgtactgca ggtcataaat
      721 tgtcagtcag ggtccctacc aaaccactgt tggaaaaaag tggtagaaat gccaccaaat
      781 ataaagtgag atggagaaat ctgcagcaat gtcagacgct tgatggtagg ataataataa
      841 ttccaaaaaa ccatcataag acattcccaa tgacagttga aggtgagttt gccgcaaaac
      901 gcttcataga agaaatggag cgctctaaag gagaatattt caactttgac attgaagtta
      961 gagatttgga ttatcttgat gctcaattga gaatttctag ctgcataaga tttggtccag
     1021 tactcacagg aaatggtgtt ttatctaaat ttctcactgg acgtagtgac cttgtaactc
     1081 ctgctgtaaa aagtatggct tggatgcttg gtctgtggtt aggtgacggt acaacaaaag
     1141 agccagaaat ctcagtagat agcttggatc ctaagctaat ggagagttta agagaaaatg
     1201 cgaaaatctg gggtctctac cttacggttt gtgacgatca cgttccgcta cgtgccaaac
     1261 atgtaaggct tcattatgga gatggtccag atgaaaacag gaagacaagg aatttgagga
     1321 aaaataatcc attctggaaa gctgtcacaa ttttaaagtt taaaagggat cttgatggag
     1381 agaagcaaat ccctgaattt atgtacggcg agcatataga agttcgtgaa gcattcttag
     1441 ccggcttgat cgactcagat gggtacgttg tgaaaaaggg cgaaggccct gaatcttata
     1501 aaatagcaat tcaaactgtt tattcatcca ttatggacgg aattgtccat atttcaagat
     1561 ctcttggtat gtcagctact gtgacgacca ggtcagctag ggaggaaatc attgaaggaa
     1621 gaaaagtcca atgtcaattt acatacgact gtaatgttgc tgggggaaca actttacaga
     1681 atgttttgtc atattgtcga agtggtcaca aaacaagaga agttccgcca attataaaaa
     1741 gggaacccgt atatttcagc ttcacggatg atttccaggg tgagagtact gtatatgggc
     1801 ttacgataga aggccataaa aatttcttgc ttggcaacaa aatagaagtg aaatcatgtc
     1861 gaggctgctg tgtgggagaa cagcataaaa tatcacaaaa aaagaatcta aaacactgtg
     1921 ttgcttgtcc cagaaaggga atcaagtatt tttataaaga ttggagtggt aaaaatcgag
     1981 tatgtgctag atgctatgga agatacaaat tcagcggtca tcactgtata aattgcaagt
     2041 atgtaccaga agcacgtgaa gtgaaaaagg caaaagacaa aggcgaaaaa ttgggcatta
     2101 cgcccgaagg tttgccagtt aaaggaccag agtgtataaa atgtggcgga atcttacagt
     2161 ttgatgctgt ccgcgggcct cataagagtt gtggtaacaa cgcaggtgcg cgcatctgcc
     2221 ggatccccgg gttaattaac ggtggaggtg gaggtggagg tggaggtgaa caaaagctaa
     2281 tctccgagga agacttgaac ggtgaacaaa aattaatctc agaagaagac ttgaacggac
     2341 tcgacggtga acaaaagttg atttctgaag aagatttgaa cggtgaacaa aagctaatct
     2401 ccgaggaaga cttgaacggt gaacaaaaat taatctcaga agaagacttg aacggactcg
     2461 acggtgaaca aaagttgatt tctgaagaag atttgaacgg tgaacaaaag ctaatctccg
     2521 aggaagactt gaacggtgaa caaaaattaa tctcagaaga agacttgaac ggactcgacg
     2581 gtgaacaaaa gttgatttct gaagaagatt tgaacggtga acaaaagcta atctccgagg
     2641 aagacttgaa cggtgaacaa aaattaatct cagaagaaga cttgaacgga ctcgacggtg
     2701 aacaaaagtt gatttctgaa gaagatttga acggtgaaca aaagctaatc tccgaggaag
     2761 acttgaacgg tgaacaaaaa ttaatctcag aagaagactt gaacggactc gacggtgaac
     2821 aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa gctaatctcc gaggaagact
     2881 tgaacggtga acaaaaatta atctcagaag aagacttgaa cggactcgac ggtgaacaaa
     2941 agttgatttc tgaagaagat ttgaacggtg aacaaaagct aatctccgag gaagacttga
     3001 acggtgaaca aaaattaatc aatcactagt gaattcgcgc cacttctaaa taagcgaatt
     3061 tcttgagggc cgcatcatgt aattagttat gtcacgctta cattcacgcc ctccccccac
     3121 atccgctcta accgaaaagg aaggagttag acaacctgaa gtctaggtcc ctatttattt
     3181 ttttatagtt atgttagtat taagaacgtt atttatattt caaatttttc ttttttttct
     3241 gtacagacgc gtgtacgcat gtaacattat actgaaaacc ttgcttgaga aggttttggg
     3301 acgctcgaag gctttaattt gcggccaagc ttggcgtaat catggtcata gctgtttcct
     3361 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     3421 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     3481 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     3541 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3601 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3661 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3721 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3781 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3841 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3901 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3961 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     4021 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4081 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4141 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4201 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4261 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4321 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4381 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4441 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4501 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4561 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4621 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4681 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4741 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4801 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4861 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4921 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4981 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5041 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5101 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     5161 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     5221 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     5281 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     5341 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     5401 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     5461 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     5521 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattcagc
     5581 tgaagcttcg tacgctgcag gtcgacggat ccccgggtta attaaggcgc gccagatctg
     5641 tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc cagaataccc
     5701 tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc ccgtacattt
     5761 agcccataca tccccatgta taatcatttg catccataca ttttgatggc cgcacggcgc
     5821 gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct cccctcacag
     5881 acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt taggatttgc
     5941 cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac agttctcaca
     6001 tcacatccga acataaacaa ccatgggtaa ggaaaagact cacgtttcga ggccgcgatt
     6061 gaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata atgtcgggca
     6121 atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt tgtttctgaa
     6181 acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac taaactggct
     6241 gacggaattc atgcctcttc cgaccatcaa gcattttatc cgtactcctg atgatgcatg
     6301 gttactcacc actgcgatcc ccggcaaaac agcattccag gtattagaag aatatcctga
     6361 ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc attcgattcc
     6421 tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg cgcaatcacg
     6481 aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg gctggcctgt
     6541 tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt cagtcgtcac
     6601 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat
     6661 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg
     6721 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa
     6781 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagtact
     6841 gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta
     6901 gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc
     6961 tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg
     7021 ctggtcgcta tactgctgtc gattcgatac taacgccgcc atccagtgtc gaaaacgagc
     7081 tcgaattcat cgatgatatc agatccacta gtggcctatg caattctcat gtttgacagc
     7141 ttatcatcga tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc
     7201 ggtttgccag ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgca
     7261 gcttcacaga aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt
     7321 gaacttttgg attggaactc gatttctgac tgggttggaa ggcaagagag ccccgagagc
     7381 ttacatttta tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta
     7441 aatggcgtta ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct
     7501 aacaaaatag caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta
     7561 tttaagtatt gtttgtgcac ttgcctgcaa gccttttgaa aagcaagcat aaaagatcta
     7621 aacataaaat ctgtaaaata acaagatgta aagataatgc taaatcattt ggctttttga
     7681 ttgattgtac aggaaaatat acatcgcagg gggttgactt ttaccatttc accgcaatgg
     7741 aatcaaactt gttgaagaga atgttcacag gcgcatacgc tacaatgacc cgattcttgc
     7801 tagccttttc tcggtcttgc aaacaaccgc cggcagctta gtatataaat acacatgtac
     7861 atacctctct ccgtatcctc gtaatcattt tcttgtattt atcgtctttt cgctgtaaaa
     7921 actttatcac acttatctca aatacactta ttaaccgctt ttactattat cttctacgct
     7981 gacagtaata tcaaacagtg acacatatta aacacagtgg tttctttgca taaacaccat
     8041 cagcctcaag tcgtcaagta aagatttcgt gttcatgcag atagataaca atctatatgt
     8101 tgataattag cgttgcctca tcaatgcgag atccgtttaa ccggacccta gtgcacttac
     8161 cccacgttcg gtccactgtg tgccgaacat gctccttcac tattttaaca tgtggaatta
     8221 tcgatgccgg tgttattctt gctatgatag gccatcgaat gttaaaggga agatttattg
     8281 ttttgccctt taaagacgaa tttggaccgt gaatgtttaa ataattctca tgtttgacag
     8341 cttatcatcg aactctaaga ggtgatactt atttactgta aaactgtgac gataaaaccg
     8401 gaaggaagaa taagaaaact cgaactgatc tataatgcct attttctgta aagagtttaa
     8461 gctatgaaag cctcggcatt ttggccgctc ctaggtagtg ctttttttcc aaggacaaaa
     8521 cagtttcttt ttcttgagca ggttttatgt ttcggtaatc ataaacaata aataaattat
     8581 ttcatttatg tttaaaaata aaaaataaaa aagtatttta aatttttaaa aaagttgatt
     8641 ataagcatgt gaccttttgc aagcaattaa attttgcaat ttgtgattta ggcaaaagtt
     8701 actatttctg gctcgtgtaa tatatgtatg ctaatgtgaa cttttacaaa gtcgatatgg
     8761 acttagtcaa aagaaatttt cttaaaaata tatagcacta gccaatttag cacttcttta
     8821 tgagatatat tatagacttt attaagccag atttgtgtat tatatgtatt tacccggcga
     8881 atcatggaca tacattctga aataggtaat attctctatg gtgagacagc atagataacc
     8941 taggatacaa gttaaaagct agtactgttt tgcagtaatt tttttctttt ttataagaat
     9001 gttaccacct aaataagtta taaagtcaat agttaagttt gatatttgat tgtaaaatac
     9061 cgtaatatat ttgcatgatc aaaaggctca atgttgacta gccagcatgt caaccactat
     9121 attgatcacc gatattagga cttccacacc aactagtaat atgacaataa attcaagata
     9181 ttcttcatga gaatggccca gcgattatgc ggtgtgaaat accgcacaga tgcgtaagga
     9241 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     9301 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     9361 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     9421 tcttattacg atcctcgcgc cccctaccca ccgtactcgt caattccaag ggcatcggta
     9481 aacatctgct caaactcgaa gtcggccata tccagagcgc cgtagggggc ggagtcgtgg
     9541 ggggtaaatc ccggacccgg ggaatccccg tcccccaaca tgtccagatc gaaatcgtct
     9601 agcgcgtcgg catgcgccat cgccacgtcc tcgccgtcta agtggagctc gtcccccagg
     9661 ctgacatcgg tcgggggggc cgtcgacagt ctgcgcgtgt gtcccgcggg gagaaaggac
     9721 aggcgcggag ccgccagccc cgcctcttcg ggggcgtcgt cgtccgggag atcgagcagg
     9781 ccctcgatgg tagacccgta attgtttttc gtacgcgcgc ggctgtacgc ggacccactt
     9841 tcacatttaa gttgtttttc taatccgcat atgatcaatt caaggccgaa taagaaggct
     9901 ggctctgcac cttggcgatc aaataattcg atagcttgtc gtaataatgg cggcatacta
     9961 tcagtagtag gtgtttccct ttcttcttta gcgacttgat gctcttgttc ttccaatacg
    10021 caacctaaag taaagtgccc cacagcgctg agtgcatata atgcattctc tagtgaaaaa
    10081 ccttgttggc ataaaaaggc taattgattt tcgagagttt catactgttt ttctgtaggc
    10141 cgtgtaccta aatgtacttt tgctccatcg cgatgactta gtaaagcaca tctaaaactt
    10201 ttagcgttat tacgtaaaaa atcttgccag ctttcccctt ctaaagggca aaagtgagta
    10261 tggtgcctat ctaacatctc aatgggtaag gcgtcgagca aagcccgctt attttttaca
    10321 tgccaataca atgtaggctg ctctacacct agcttctggg cgagtttacg ggttgttaaa
    10381 ccttcgattc cgaccccatt aagcagctct aatgcgccgt taatcacttt acttttatct
    10441 aatctagaca tatgaattaa ttcggggccg cggaggctgg atcggtcccg gtgtcttcta
    10501 tggaggtcaa aacagcgtgg atggcgtctc caggcgatct gacggttcac taaacgagct
    10561 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
    10621 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
    10681 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
    10741 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa
    10801 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
    10861 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
    10921 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
    10981 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
    11041 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
    11101 gtaattgatt actattaata actagtcaat aatcaatgtc aacatggcgg taatgttgga
    11161 catgagccaa tataaatgta catattatga tatggataca acgtatgcaa tgggccaagc
    11221 tcctcgagta attcgcgcca cttctaataa gcgatttctt atgatttatg atttttatta
    11281 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa
    11341 aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat
    11401 agtatgaggt cgctcttatt gaccacacct ctaccggcag atccgctagg gataacaggg
    11461 taatatagat caattcctcg atcgagttta ccac
//
