LOCUS       40924_24962        9233 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHRGFPTC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9233)
  AUTHORS   Quandt J, Hynes MF.
  TITLE     Versatile suicide vectors which allow direct selection for gene 
            replacement in gram-negative bacteria
  JOURNAL   Gene 127 (1), 15-21 (1993)
  PUBMED    8486283
REFERENCE   2  (bases 1 to 9233)
  AUTHORS   Dennis JJ, Zylstra GJ.
  TITLE     Plasposons: modular self-cloning minitransposon derivatives for 
            rapid genetic analysis of gram-negative bacterial genomes
  JOURNAL   Appl. Environ. Microbiol. 64 (7), 2710-2715 (1998)
  PUBMED    9647854
REFERENCE   3  (bases 1 to 9233)
  AUTHORS   Ouahrani-Bettache S, Porte F, Teyssier J, Liautard JP, Kohler S.
  TITLE     pBBR1-GFP: a broad-host-range vector for prokaryotic promoter 
            studies
  JOURNAL   BioTechniques 26 (4), 620-622 (1999)
  PUBMED    10343896
REFERENCE   4  (bases 1 to 9233)
  AUTHORS   Ramos HJ, Roncato-Maccari LD, Souza EM, Soares-Ramos JR, Hungria M, 
            Pedrosa FO.
  TITLE     Monitoring Azospirillum-wheat interactions using the gfp and gusA 
            genes constitutively expressed from a new broad-host range vector
  JOURNAL   J. Biotechnol. 97 (3), 243-252 (2002)
  PUBMED    12084480
REFERENCE   5  (bases 1 to 9233)
  AUTHORS   Ramos HJO., Soares-Ramos JRL., Souza EM, Pedrosa FO.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2003) Department of Biochemistry and Molecular 
            Biology, Universidade Federal do Parana - UFPR, Centro Politecnico, 
            Curitiba, PR 81531-990, Brazil
REFERENCE   6  (bases 1 to 9233)
  TITLE     Direct Submission
REFERENCE   7  (bases 1 to 9233)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1993"; volume: "127"; issue: "1"; pages: "15-21"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Appl. 
            Environ. Microbiol."; date: "1998"; volume: "64"; issue: "7"; pages:
            "2710-2715"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1999"; volume: "26"; issue: "4"; pages: 
            "620-622"
COMMENT     SGRef: number: 4; type: "Journal Article"; journalName: "J. 
            Biotechnol."; date: "2002"; volume: "97"; issue: "3"; pages: 
            "243-252"
COMMENT     SGRef: number: 5; type: "Journal Article"; journalName: "Submitted 
            (14-FEB-2003) Department of Biochemistry and Molecular Biology, 
            Universidade Federal do Parana - UFPR, Centro Politecnico, Curitiba,
            PR 81531-990, Brazil"
COMMENT     SGRef: number: 6; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9233
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2452
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     primer_bind     2614..2630
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2664..2680)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3110..3130)
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     intron          complement(3260..3325)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(3494..4150)
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             complement(4216..4929)
                     /codon_start=1
                     /label=yeGFP
                     /note="yeast-enhanced green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     misc_feature    4966..5465
                     /label=from pJQ200KS
                     /note="from pJQ200KS"
     promoter        complement(5473..5501)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    6010..6031
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        6046..6076
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6084..6100
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6108..6124
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6145..6163
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6192..6221
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
     CDS             6269..7456
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     CDS             complement(8165..9022)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccccaccg cggtggcggc cgctctagaa ctagtggatc ccccgggctg
     2641 caggaattcg atatcaagct tatcgatacc gtcgacctcg aggtcaacaa caacaattgc
     2701 attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac
     2761 ctctacaaat gtggtatggc tgattatgat ctctagtcaa ggcactatac atcaaatatt
     2821 ccttattaac ccctttacaa attaaaaagc taaaggtaca caatttttga gcatagttat
     2881 taatagcaga cactctatgc ctgtgtggag taagaaaaaa cagtatgtta tgattataac
     2941 tgttatgcct acttataaag gttacagaat atttttccat aattttcttg tatagcagtg
     3001 cagctttttc ctttgtggtg taaatagcaa agcaagcaag agttctatta ctaaacacag
     3061 catgactcaa aaaacttagc aattctgaag gaaagtcctt ggggtcttct acctttctct
     3121 tcttttttgg aggagtagaa tgttgagagt cagcagtagc ctcatcatca ctagatggca
     3181 tttcttctga gcaaaacagg ttttcctcat taaaggcatt ccaccactgc tcccattcat
     3241 cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt
     3301 atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca
     3361 attatgtcac accacagaag taaggttcct tcacaaagat ccggcgaatt tctgccattc
     3421 atccgcttat tatcacttat tcaggcgtag caccaggcgt ttaagggcac caataactgc
     3481 cttaaaaaaa ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca
     3541 ttctgccgac atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca
     3601 gcaccttgtc gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt
     3661 ccatattggc cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga
     3721 aaaacatatt ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca
     3781 catcttgcga atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg
     3841 atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata
     3901 tcaccagctc accgtctttc attgccatac ggaattccgg atgagcattc atcaggcggg
     3961 caagaatgtg aataaaggcc ggataaaact tgtgcttatt tttctttacg gtctttaaaa
     4021 aggccgtaat atccagctga acggtctggt tataggtaca ttgagcaact gactgaaatg
     4081 cctcaaaatg ttctttacga tgccattggg atatatcaac ggtggtatat ccagtgattt
     4141 ttttctccat tttagcttcc ttagctcctg aaaatctcgc caagcttgat atcgaattcc
     4201 tgcaggtctg gactatttgt atagttcatc catgccatgt gtaatcccag cagctgttac
     4261 aaactcaaga aggaccatgt ggtctctctt ttcgttggga tctttcgaaa gggcagattg
     4321 tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg gagtattttg
     4381 ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtgtctaa ttttgaagtt
     4441 aactttgatt ccattctttt gtttgtctgc catgatgtat acattgtgtg agttatagtt
     4501 gtattccaat ttgtgtccaa gaatgtttcc atcttcttta aaatcaatac cttttaactc
     4561 gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct tgtagttccc
     4621 gtcatctttg aaaaatatag ttctttcctg tacataacct tcgggcatgg cactcttgaa
     4681 aaagtcatgc tgtttcatat gatctgggta tctcgcaaag cattgaacac cataaccgaa
     4741 agtagtgaca agtgttggcc atggaacagg tagttttcca gtagtgcaaa taaatttaag
     4801 ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaaa atttgtgccc
     4861 attaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaaa gttcttctcc
     4921 tttactcata tgtatatctc cttcttaaat ctagatttaa agatcagatc tcactacgcg
     4981 gctgctcaaa cctgggcaga acgtaagccg cgagagcgcc aacaaccgct tcttggtcga
     5041 aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa tcggagtccg
     5101 gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga tcaagagcag
     5161 cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg cccatacttg
     5221 agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta
     5281 acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg
     5341 gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa aaccgccact
     5401 gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga gcgcatacgc
     5461 tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg tgccttcatc
     5521 cgtttccacg gtgtgcgtca cccggcaacc ttgggcagca gcgaagtcga ggcatttctg
     5581 tcctggctgg cgaacgagcg caaggtttcg gtctccacgc atcgtcaggc attggcggcc
     5641 ttgctgttct tctacggcaa ggtgctgtgc acggatctgc cctggcttca ggagatcgga
     5701 agacctcggc cgtcgcggcg cttgccggtg gtgctgaccc cggatgaagt ggttcgcatc
     5761 ctcggttttc tggaaggcga gcatcgtttg ttcgcccagc ttctgtatgg aacgggcatg
     5821 cggatcagtg agggtttgca actgcgggtc aaggatctgg atttcgatca cggcacgatc
     5881 atcgtgcggg agggcaaggg ctccaaggat cgggccttga tgttacccga gagcttggca
     5941 cccacgctgc gcgagcccct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     6001 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     6061 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     6121 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctgggtac
     6181 cgagctcatg tttgacagct tatcatccga ttagctttaa tgcggtagtt tatcacagtt
     6241 aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca tcgtcatcct
     6301 cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac tgccgggcct
     6361 cttgcgggat atcgtccatt ccgacagcat cgccagtcac tatggcgtgc tgctagcgct
     6421 atatgcgttg atgcaatttc tatgcgcacc cgttctcgga gcactgtccg accgctttgg
     6481 ccgccgccca gtcctgctcg cttcgctact tggagccact atcgactacg cgatcatggc
     6541 gaccacaccc gtcctgtgga ttctctacgc cggacgcatc gtggccggca tcaccggcgc
     6601 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     6661 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     6721 gggactgttg ggcgccatct ccttacatgc accattcctt gcggcggcgg tgctcaacgg
     6781 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgccgacc
     6841 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat
     6901 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc
     6961 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc
     7021 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac
     7081 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta
     7141 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc
     7201 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga
     7261 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg
     7321 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg
     7381 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag
     7441 ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca
     7501 agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccgagctcg
     7561 gtaccgggcc ccccctcgag ggggggcccg tttctcaaag ctcacgctgt aggtatctca
     7621 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     7681 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     7741 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     7801 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     7861 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     7921 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     7981 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     8041 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     8101 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     8161 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     8221 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     8281 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     8341 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     8401 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     8461 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     8521 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     8581 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     8641 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     8701 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     8761 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     8821 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     8881 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     8941 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     9001 cacggaaatg ttgaatactc atactcttcc tttttcaatc aggtggcact tttcggggaa
     9061 atgtgcgcgc ccgcgttcct gctggcgctg ggcctgtttc tggcgctgga cttcccgctg
     9121 ttccgtcagc agcttttcgc ccacggcctt gatgatcgcg gcggccttgg cctgcatatc
     9181 ccgattcaac ggccccaggg cgtccagaac gggcttcagg cgctcccgaa ggt
//
