LOCUS       40924_24912       10725 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHR307a, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10725)
  AUTHORS   Mastick GS, McKay R, Oligino T, Donovan K, Lopez AJ.
  TITLE     Identification of target genes regulated by homeotic proteins in 
            Drosophila melanogaster through genetic selection of Ultrabithorax 
            protein-binding sites in yeast
  JOURNAL   Genetics 139 (1), 349-363 (1995)
  PUBMED    7705635
REFERENCE   2  (bases 1 to 10725)
  AUTHORS   Hollenbach AD, Ng S-S., Flynn AP.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-APR-2005) Genetics, Louisiana State University Health 
            Sciences Center, 533 Bolivar Street, New Orleans, LA 70119, USA
REFERENCE   3  (bases 1 to 10725)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10725)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "1995"; volume: "139"; issue: "1"; pages: "349-363"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-APR-2005) Genetics, Louisiana State University Health Sciences 
            Center, 533 Bolivar Street, New Orleans, LA 70119, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10725
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..50
                     /label=multiple cloning site
                     /note="multiple cloning site"
     regulatory      51..360
                     /label=GAL1 minimal promoter
                     /note="GAL1 minimal promoter"
                     /regulatory_class="promoter"
     CDS             366..1025
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     3'UTR           1029..1944
                     /gene="HIS3"
     promoter        2738..2953
                     /label=URA3 promoter
     3'UTR           complement(3160..3848)
                     /gene="TRP1"
     rep_origin      3173..4009
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     CDS             complement(3896..4522)
                     /codon_start=1
                     /gene="TRP1"
                     /product="TRP1"
                     /label=TRP1
                     /protein_id="AAY40260.1"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGGRQMV"
     promoter        complement(4523..4624)
                     /label=TRP1 promoter
     misc_feature    complement(6576..8436)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     misc_feature    8575..8715
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8901..9489)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9663..10520)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(10521..10625)
                     /label=AmpR promoter
ORIGIN
        1 gaattccccg gatccgttga cctgcaggtc gacggatccg gggaattcct ggccccacaa
       61 accttcaaat gaacgaatca aattaacaac cataggatga taatgcgatt agttttttag
      121 ccttatttct ggggtaatta atcagcgaag cgatgatttt tgatctatta acagatatat
      181 aaatgcaaaa actgcataac cactttaact aatactttca acattttcgg tttgtattac
      241 ttcttattca aatgtaataa aagtatcaac aaaaaattgt taatatacct ctatacttta
      301 acgtcaagga gaaaaaaccc cggatcaatt ccaaaaatga gcaggcaaga taaacgaagg
      361 caaagatgac agagcagaaa gccctagtaa agcgtattac aaatgaaacc aagattcaga
      421 ttgcgatctc tttaaagggt ggtcccctag cgatagagca ctcgatcttc ccagaaaaag
      481 aggcagaagc agtagcagaa caggccacac aatcgcaagt gattaacgtc cacacaggta
      541 tagggtttct ggaccatatg atacatgctc tggccaagca ttccggctgg tcgctaatcg
      601 ttgagtgcat tggtgactta cacatagacg accatcacac cactgaagac tgcgggattg
      661 ctctcggtca agcttttaaa gaggccctag gggccgtgcg tggagtaaaa aggtttggat
      721 caggatttgc gcctttggat gaggcacttt ccagagcggt ggtagatctt tcgaacaggc
      781 cgtacgcagt tgtcgaactt ggtttgcaaa gggagaaagt aggagatctc tcttgcgaga
      841 tgatcccgca ttttcttgaa agctttgcag aggctagcag aattaccctc cacgttgatt
      901 gtctgcgagg caagaatgat catcaccgta gtgagagtgc gttcaaggct cttgcggttg
      961 ccataagaga agccacctcg cccaatggta ccaacgatgt tccctccacc aaaggtgttc
     1021 ttatgtagtg acaccgatta tttaaagctg cagcatacga tatatataca tgtgtatata
     1081 tgtataccta tgaatgtcag taagtatgta tacgaacagt atgatactga agatgacaag
     1141 gtaatgcatc attctatacg tgtcattctg aacgaggcgc gctttccttt tttctttttg
     1201 ctttttcttt ttttttctct tgaactcgag aaaaaaaata taaaagagat ggaggaacgg
     1261 gaaaaagtta gttgtggtga taggtggcaa gtggtattcc gtaagaacaa caagaaaagc
     1321 atttcatatt atggctgaac tgagcgaaca agtgcaaaat ttaagcatca acgacaacaa
     1381 cgagaatggt tatgttcctc ctcacttaag aggaaaacca agaagtgcca gaaataacag
     1441 tagcaactac aataacaaca acggcggcta caacggtggc cgtggcggtg gcagcttctt
     1501 tagcaacaac cgtcgtggtg gttacggcaa cggtggtttc ttcggtggaa acaacggtgg
     1561 cagcagatct aacggccgtt ctggtggtag atggatcgat ggcaaacatg tcccagctcc
     1621 aagaaacgaa aaggccgaga tcgccatatt tggtgtcccc gaggatcgat cctctacgcc
     1681 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     1741 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     1801 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     1861 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     1921 caggagtcgc ataagggaga gcgtcgaccg atgcccttga gagccttcaa cccagtcagc
     1981 tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc
     2041 atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga ggaccgcttt
     2101 cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt gcacgccctc
     2161 gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca ggccattatc
     2221 gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg
     2281 atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc cgcgttgcag
     2341 gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg atcgctcgcg
     2401 gctcttacca gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc
     2461 tcggcgagca catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc
     2521 ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc
     2581 ggcacctcgc taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa
     2641 ctgtgaatgc gcaaaccaac ccttggcaga acatatccat cgcgtccgcc atctccagca
     2701 gccgcacgcg ggcgcatcgg gggggggggg ggggggtttc aattcatcat ttttttttta
     2761 ttcttttttt tgatttcggt ttccttgaaa tttttttgat tcggtaatct ccgaacagaa
     2821 ggaagaacga aggaaggagc acagacttag attggtatat atacgcatat gtagtgttga
     2881 agaaacatga aattgcccag tattcttaac ccaactgcac agaacaaaaa cctgcaggaa
     2941 acgaagataa atcatgtcga aagctacata taaggaacgt gctgctactc atcctagtcc
     3001 tgttgctgcc aagctattta atatcatgca cgaaaagcaa acaaacttgt gtgcttcatt
     3061 ggatgttcgt accaccaagg aattactgga gttagttgaa gcattaggtc ccaaaatttg
     3121 tttactaaaa acacatgtgg atatcttgac tgatttttcc atggaattaa ttccacatgt
     3181 taaaatagtg aaggagcatg ttcggcacac agtggaccga acgtggggta agtgcactag
     3241 ggtccggtta aacggatctc gcattgatga ggcaacgcta attatcaaca tatagattgt
     3301 tatctatctg catgaacacg aaatctttac ttgacgactt gaggctgatg gtgtttatgc
     3361 aaagaaacca ctgtgtttaa tatgtgtcac tgtttgatat tactgtcagc gtagaagata
     3421 atagtaaaag cggttaataa gtgtatttga gataagtgtg ataaagtttt tacagcgaaa
     3481 agacgataaa tacaagaaaa tgattacgag gatacggaga gaggtatgta catgtgtatt
     3541 tatatactaa gctgccggcg gttgtttgca agaccgagaa aaggctagca agaatcgggt
     3601 cattgtagcg tatgcgcctg tgaacattct cttcaacaag tttgattcca ttgcggtgaa
     3661 atggtaaaag tcaaccccct gcgatgtata ttttcctgta caatcaatca aaaagccaaa
     3721 tgatttagca ttatctttac atcttgttat tttacagatt ttatgtttag atcttttatg
     3781 cttgcttttc aaaaggcctg caggcaagtg cacaaacaat acttaaataa atactactca
     3841 gtaataacct atttcttagc atttttgacg aaatttgcta ttttgttaga gtcttttaca
     3901 ccatttgtct cccacctccg cttacatcaa caccaataac gccatttaat ctaagcgcat
     3961 caccaacatt ttctggcgtc agtccaccag ctaacataaa atgtaagctt tcggggctct
     4021 cttgccttcc aacccagtca gaaatcgagt tccaatccaa aagttcacct gtcccacctg
     4081 cttctgaatc aaacaaggga ataaacgaat gaggtttctg tgaagctgca ctgagtagta
     4141 tgttgcagtc ttttggaaat acgagtcttt taataactgg caaaccgagg aactcttggt
     4201 attcttgcca cgactcatct ccatgcagtt ggacgatatc aatgccgtaa tcattgacca
     4261 gagccaaaac atcctcctta ggttgattac gaaacacgcc aaccaagtat ttcggagtgc
     4321 ctgaactatt tttatatgct tttacaagac ttgaaatttt ccttgcaata accgggtcaa
     4381 ttgttctctt tctattgggc acacatataa tacccagcaa gtcagcatcg gaatctagag
     4441 cacattctgc ggcctctgtg ctctgcaagc cgcaaacttt caccaatgga ccagaactac
     4501 ctgtgaaatt aataacagac atactccaag ctgcctttgt gtgcttaatc acgtatactc
     4561 acgtgctcaa tagtcaccaa tgccctccct cttggccctc tccttttctt ttttcgaccg
     4621 aattaattcc atggagggca cagttaagcc gctaaaggca ttatccgcca agtacaattt
     4681 tttactcttc gaagacagaa aatttgctga cattggtaat acagtcaaat tgcagtactc
     4741 tgcgggtgta tacagaatag cagaatgggc agacattacg aatgcacacg gtgtggtggg
     4801 cccaggtatt gttagcggtt tgaagcaggc ggcagaagaa gtaacaaagg aacctagagg
     4861 ccttttgatg ttagcagaat tgtcatgcaa gggctcccta tctactggaa gaatatacta
     4921 agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt attgctcaaa
     4981 gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc ggtgtgggtt
     5041 tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat gtggtctcta
     5101 caggatctga cattattatt gttggaagag gactatttgc aaagggaagg gatgctaagg
     5161 tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga tgcggccagc
     5221 aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa attagagctt
     5281 caatttaatt atatcagtta ttacccggga atctcggtcg taatgatttt tataatgacg
     5341 aaaaaaaaaa aattggaaag aaaacccccc cccccccccc cccgcagcgt tgggtcctgg
     5401 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc
     5461 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc
     5521 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt
     5581 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc
     5641 tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga
     5701 gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc
     5761 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc
     5821 ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag
     5881 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag
     5941 aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac
     6001 gctgatgagc tttaccgctg ggccattctc atgaagaata tcttgaattt attgtcatat
     6061 tactagttgg tgtggaagtc ctaatatcgg tgatcaatat agtggttgac atgctggcta
     6121 gtcaacattg agccttttga tcatgcaaat atattacggt attttacaat caaatatcaa
     6181 acttaactat tgactttata acttatttag gtggtaacat tcttataaaa aagaaaaaaa
     6241 ttactgcaaa acagtactag cttttaactt gtatcctagg ttatctatgc tgtctcacca
     6301 tagagaatat tacctatttc agaatgtatg tccatgattc gccgggtaaa tacatataat
     6361 acacaaatct ggcttaataa agtctataat atatctcata aagaagtgct aaattggcta
     6421 gtgctatata tttttaagaa aatttctttt gactaagtcc atatcgactt tgtaaaagtt
     6481 cacattagca tacatatatt acacgagcca gaaatagtaa cttttgccta aatcacaaat
     6541 tgcaaaattt aattgcttgc aaaaggtcac atgcttataa tcaacttttt taaaaattta
     6601 aaatactttt ttatttttta tttttaaaca taaatgaaat aatttattta ttgtttatga
     6661 ttaccgaaac ataaaacctg ctcaagaaaa agaaactgtt ttgtccttgg aaaaaaagca
     6721 ctacctagga gcggccaaaa tgccgaggct ttcatagctt aaactcttta cagaaaatag
     6781 gcattataga tcagttcgag ttttcttatt cttccttccg gttttatcgt cacagtttta
     6841 cagtaaataa gtatcacctc ttagagttaa cctatactaa aatttatgcc agttaactat
     6901 gagataagca agtatcatct catttcatta cctgaagtcg agtaaacaga aaatccaatt
     6961 gttgatgaac ctcaatgact tagaactatc tatcggcaga tcatataaag aggatttagg
     7021 tacctagagg actgtacctg gagtatatat atatatatat atatattatc tcaactatag
     7081 tccatagagg tttctttctt gaggccttaa actgctaaag aatgatattg gtggaatgca
     7141 agcaccaagc gctcttcttg cgtgactgtt catatacttc aaaccaagaa tgtaactggc
     7201 attgacccat ccaaaacctt cagtagctgc ccctttaaag tcagcacctt gattaccgta
     7261 ttctgcttca acacgatgag gatctgttcc tcttgtgaca tcatattttt caaccacaat
     7321 accattataa tcgacaaaag cctttgtcat catgaaaagc catctataag ctagcctatt
     7381 cgttacagtt aaataaccat aagaacggag gccttcccaa gcaagaattt gatggggtgc
     7441 ccaaccaaat ggatagtccc attgtctaat tggtctcgaa atagaaattg ggcctcgaga
     7501 acgctccgta catgcagcta aacctccaag catctctaac ttgggtagtg ctttctccac
     7561 cattttctgt gcttgctcct tcgtggcaag tccagcccat aatgcccaga atgtagttgc
     7621 ggattcgtat gacgttctgt gcttgatttt tgtgttgtag tcaaagaaaa ggtccggtta
     7681 aacggatctc gcattgatga ggcaacgcta attatcaaca tatagattgt tatctatctg
     7741 catgaacacg aaatctttac ttgacgactt gaggctgatg gtgtttatgc aaagaaacca
     7801 ctgtgtttaa tatgtgtcac tgtttgatat tactgtcagc gtagaagata atagtaaaag
     7861 cggttaataa gtgtatttga gataagtgtg ataaagtttt tacagcgaaa agacgataaa
     7921 tacaagaaaa tgattacgag gatacggaga gaggtatgta catgtgtatt tatatactaa
     7981 gctgccggcg gttgtttgca agaccgagaa aaggctagca agaatcgggt cattgtagcg
     8041 tatgcgcctg tgaacattct cttcaacaag tttgattcca ttgcggtgaa atggtaaaag
     8101 tcaaccccct gcgatgtata ttttcctgta caatcaatca aaaagccaaa tgatttagca
     8161 ttatctttac atcttgttat tttacagatt ttatgtttag atcttttatg cttgcttttc
     8221 aaaaggcctg caggcaagtg cacaaacaat acttaaataa atactactca gtaataacct
     8281 atttcttagc atttttgacg aaatttgcta ttttgttaga gtcttttaca ccatttgtct
     8341 ccacacctcc gcttacatca acaccaataa cgccatttaa tctaagcgca tcaccaacat
     8401 tttctggcgt cagtccacca gctaacataa aatgttgcct cgcgcgtttc ggtgatgacg
     8461 gtgaaaacct ttgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     8521 ccggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
     8581 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
     8641 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     8701 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     8761 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8821 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8881 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8941 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     9001 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     9061 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     9121 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     9181 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     9241 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     9301 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     9361 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     9421 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     9481 ggatctcaag aagatccttt gatcttttct ncggggtctg acgctcagtg gaacgaaaac
     9541 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     9601 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     9661 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     9721 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9781 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9841 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9901 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9961 gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    10021 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    10081 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    10141 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    10201 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    10261 tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    10321 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    10381 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    10441 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    10501 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    10561 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    10621 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
    10681 ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaa
//
