LOCUS       40924_24902       10706 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHR28A10, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10706)
  AUTHORS   Ramos HJ, Souza EM, Soares-Ramos JR, Pedrosa FO.
  TITLE     A new system to control the barnase expression by a NifA-dependent 
            promoter
  JOURNAL   J. Biotechnol. 118 (1), 9-16 (2005)
  PUBMED    15908028
REFERENCE   2  (bases 1 to 10706)
  AUTHORS   Ramos HJO., Soares-Ramos JRL., Souza EM, Pedrosa FO.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-FEB-2003) Department of Biochemistry and Molecular 
            Biology, Universidade Federal do Parana - UFPR, Centro Politecnico, 
            Curitiba, Parana 81531-990, Brazil
REFERENCE   3  (bases 1 to 10706)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10706)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. 
            Biotechnol."; date: "2005"; volume: "118"; issue: "1"; pages: "9-16"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (14-FEB-2003) Department of Biochemistry and Molecular Biology, 
            Universidade Federal do Parana - UFPR, Centro Politecnico, Curitiba,
            Parana 81531-990, Brazil"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1023..1792
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1793..2452
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
     CDS             complement(3047..3835)
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     regulatory      4744..4776
                     /label=ptac
                     /note="ptac"
                     /regulatory_class="promoter"
     promoter        4746..4773
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    4781..4797
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             4863..6671
                     /codon_start=1
                     /gene="uidA"
                     /product="beta-glucuronidase"
                     /label=uidA
                     /note="expression regulated by ptac; derived from pMC110"
                     /protein_id="AAO92243.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAI
                     AVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEV
                     MEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHD
                     FFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQ
                     VVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQF
                     LINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDW
                     ADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARD
                     KNHPSVVMWSIANEPDTRPQVHGNISPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
                     FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
                     DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
                     KSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            4863..6671
                     /gene="uidA"
                     /label=uidA
     regulatory      6735..6763
                     /label=trpA
                     /note="trpA"
                     /regulatory_class="terminator"
     primer_bind     6798..6814
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6842..6860)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6879..6895)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6903..6919
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6927..6957)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6972..6993)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(7009..8088)
                     /label=lacI
                     /note="lac repressor"
     CDS             complement(8165..8437)
                     /codon_start=1
                     /gene="barstar"
                     /product="barnase inhibitor"
                     /label=barstar
                     /note="expression regulated by pnifH"
                     /protein_id="AAO92245.1"
                     /translation="MKKAVINGEQIRSISDLHQTLKKELALPEYYGENLDALWDCLTGW
                     VEYPLVLEWRQFEQSKQLTENGAESVLQVFREAKAEGCDITIILS"
     gene            complement(8165..8437)
                     /gene="barstar"
                     /label=barstar
                     /note="promoterless barstar gene derived from pMT316
                     deposited in GenBank Accession Number X12871"
     regulatory      complement(8608..8757)
                     /note="Sinorhizobium meliloti nifH -24/-12 type promoter
                     derived from pMCK28"
                     /regulatory_class="promoter"
     misc_feature    complement(8761..9053)
                     /label=derived from pMCK28
                     /note="derived from pMCK28"
     CDS             complement(9638..10495)
                     /label=AmpR
                     /note="beta-lactamase"
ORIGIN
        1 ctcgggccgt ctcttgggct tgatcggcct tcttgcgcat ctcacgcgct cctgcggcgg
       61 cctgtagggc aggctcatac ccctgccgaa ccgcttttgt cagccggtcg gccacggctt
      121 ccggcgtctc aacgcgcttt gagattccca gcttttcggc caatccctgc ggtgcatagg
      181 cgcgtggctc gaccgcttgc gggctgatgg tgacgtggcc cactggtggc cgctccaggg
      241 cctcgtagaa cgcctgaatg cgcgtgtgac gtgccttgct gccctcgatg ccccgttgca
      301 gccctagatc ggccacagcg gccgcaaacg tggtctggtc gcgggtcatc tgcgctttgt
      361 tgccgatgaa ctccttggcc gacagcctgc cgtcctgcgt cagcggcacc acgaacgcgg
      421 tcatgtgcgg gctggtttcg tcacggtgga tgctggccgt cacgatgcga tccgccccgt
      481 acttgtccgc cagccacttg tgcgccttct cgaagaacgc cgcctgctgt tcttggctgg
      541 ccgacttcca ccattccggg ctggccgtca tgacgtactc gaccgccaac acagcgtcct
      601 tgcgccgctt ctctggcagc aactcgcgca gtcggcccat cgcttcatcg gtgctgctgg
      661 ccgcccagtg ctcgttctct ggcgtcctgc tggcgtcagc gttgggcgtc tcgcgctcgc
      721 ggtaggcgtg cttgagactg gccgccacgt tgcccatttt cgccagcttc ttgcatcgca
      781 tgatcgcgta tgccgccatg cctgcccctc ccttttggtg tccaaccggc tcgacggggg
      841 cagcgcaagg cggtgcctcc ggcgggccac tcaatgcttg agtatactca ctagactttg
      901 cttcgcaaag tcgtgaccgc ctacggcggc tgcggcgccc tacgggcttg ctctccgggc
      961 ttcgccctgc gcggtcgctg cgctcccttg ccagcccgtg gatatgtgga cgatggccgc
     1021 gagcggccac cggctggctc gcttcgctcg gcccgtggac aaccctgctg gacaagctga
     1081 tggacaggct gcgcctgccc acgagcttga ccacagggat tgcccaccgg ctacccagcc
     1141 ttcgaccaca tacccaccgg ctccaactgc gcggcctgcg gccttgcccc atcaattttt
     1201 ttaattttct ctggggaaaa gcctccggcc tgcggcctgc gcgcttcgct tgccggttgg
     1261 acaccaagtg gaaggcgggt caaggctcgc gcagcgaccg cgcagcggct tggccttgac
     1321 gcgcctggaa cgacccaagc ctatgcgagt gggggcagtc gaaggcgaag cccgcccgcc
     1381 tgccccccga gcctcacggc ggcgagtgcg ggggttccaa gggggcagcg ccaccttggg
     1441 caaggccgaa ggccgcgcag tcgatcaaca agccccggag gggccacttt ttgccggagg
     1501 gggagccgcg ccgaaggcgt gggggaaccc cgcaggggtg cccttctttg ggcaccaaag
     1561 aactagatat agggcgaaat gcgaaagact taaaaatcaa caacttaaaa aaggggggta
     1621 cgcaacagct cattgcggca ccccccgcaa tagctcattg cgtaggttaa agaaaatctg
     1681 taattgactg ccacttttac gcaacgcata attgttgtcg cgctgccgaa aagttgcagc
     1741 tgattgcgca tggtgccgca accgtgcggc accctaccgc atggagataa gcatggccac
     1801 gcagtccaga gaaatcggca ttcaagccaa gaacaagccc ggtcactggg tgcaaacgga
     1861 acgcaaagcg catgaggcgt gggccgggct tattgcgagg aaacccacgg cggcaatgct
     1921 gctgcatcac ctcgtggcgc agatgggcca ccagaacgcc gtggtggtca gccagaagac
     1981 actttccaag ctcatcggac gttctttgcg gacggtccaa tacgcagtca aggacttggt
     2041 ggccgagcgc tggatctccg tcgtgaagct caacggcccc ggcaccgtgt cggcctacgt
     2101 ggtcaatgac cgcgtggcgt ggggccagcc ccgcgaccag ttgcgcctgt cggtgttcag
     2161 tgccgccgtg gtggttgatc acgacgacca ggacgaatcg ctgttggggc atggcgacct
     2221 gcgccgcatc ccgaccctgt atccgggcga gcagcaacta ccgaccggcc ccggcgagga
     2281 gccgcccagc cagcccggca ttccgggcat ggaaccagac ctgccagcct tgaccgaaac
     2341 ggaggaatgg gaacggcgcg ggcagcagcg cctgccgatg cccgatgagc cgtgttttct
     2401 ggacgatggc gagccgttgg agccgccgac acgggtcacg ctgccgcgcc ggtagcactt
     2461 gggttgcgca gcaacccgta agtgcgctgt tccagactat cggctgtagc cgcctcgccg
     2521 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga
     2581 cctgaatgga agccccaccg cggtggcggc cgctctagac tgtctcttga tcagatctgg
     2641 ccgcctaggc cgaattcccc gggatccaag cttgatgctt gtaaaccgtt ttgtgaaaaa
     2701 atttttaaaa taaaaaaggg gacctctagg gtccccaatt aattagtaat ataatctatt
     2761 aaaggtcatt caaaaggtca tccaccggat cagcttagta aagccctcgc tagattttaa
     2821 tgcggatgtt gcgattactt cgccaactat tgcgataaca agaaaaagcc agcctttcat
     2881 gatatatctc ccaatttgtg tagggcttat tatgcacgct taaaaataat aaaagcagac
     2941 ttgacctgat agtttggctg tgagcaatta tgtgcttagt gcatctaacg cttgagttaa
     3001 gccgcgccgc gaagcggcgt cggcttgaac gaattgttag acattatttg ccgactacct
     3061 tggtgatctc gcctttcacg tagtggacaa attcttccaa ctgatctgcg cgcgaggcca
     3121 agcgatcttc ttcttgtcca agataagcct gtctagcttc aagtatgacg ggctgatact
     3181 gggccggcag gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg
     3241 ttactgcgct gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc
     3301 agtcgggcgg cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt
     3361 caggaaccgg atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc
     3421 ttgcttttgt cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg
     3481 caagaatgtc attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc
     3541 acggaatgat gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct
     3601 ctccagggga agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat
     3661 caagccttac ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat
     3721 ccactgcgga gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga
     3781 cgccaactac ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt
     3841 ttaactttgt tttagggcga ctgccctgct gcgtaacatc gttgctgctc cataacatca
     3901 aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc ccgaggcata gactgtaccc
     3961 caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt
     4021 cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta cttgcattac agcttacgaa
     4081 ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc
     4141 cggcaacctt gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca
     4201 aggtttcggt ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg
     4261 tctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     4321 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     4381 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     4441 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     4501 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcaggggaa
     4561 ttgatccggt ggatgacctt ttgaatgacc tttaatagat tatattacta attaattggg
     4621 gaccctagag gtcccctttt ttattttaaa aattttttca caaaacggtt tacaagcatc
     4681 aagcttggat ccccggggaa ttcggccgcc taggccgcgg ccgcaagctt actccccatc
     4741 ccctgttgac aattaatcat cggctcgtat aattgtggaa ttgtgagcgg ataacaattt
     4801 cacacaggaa acaggatccg tcaattccgg accagtatta ttatcttaat gaggagtccc
     4861 ttatgttacg tcctgtagaa accccaaccc gtgaaatcaa aaaactcgac ggcctgtggg
     4921 cattcagtct ggatcgcgaa aactgtggaa ttgatcagcg ttggtgggaa agcgcgttac
     4981 aagaaagccg ggcaattgct gtgccaggca gttttaacga tcagttcgcc gatgcagata
     5041 ttcgtaatta tgcgggcaac gtctggtatc agcgcgaagt ctttataccg aaaggttggg
     5101 caggccagcg tatcgtgctg cgtttcgatg cggtcactca ttacggcaaa gtgtgggtca
     5161 ataatcagga agtgatggag catcagggcg gctatacgcc atttgaagcc gatgtcacgc
     5221 cgtatgttat tgccgggaaa agtgtacgta tcaccgtttg tgtgaacaac gaactgaact
     5281 ggcagactat cccgccggga atggtgatta ccgacgaaaa cggcaagaaa aagcagtctt
     5341 acttccatga tttctttaac tatgccggga tccatcgcag cgtaatgctc tacaccacgc
     5401 cgaacacctg ggtggacgat atcaccgtgg tgacgcatgt cgcgcaagac tgtaaccacg
     5461 cgtctgttga ctggcaggtg gtggccaatg gtgatgtcag cgttgaactg cgtgatgcgg
     5521 atcaacaggt ggttgcaact ggacaaggca ctagcgggac tttgcaagtg gtgaatccgc
     5581 acctctggca accgggtgaa ggttatctct atgaactgtg cgtcacagcc aaaagccaga
     5641 cagagtgtga tatctacccg cttcgcgtcg gcatccggtc agtggcagtg aagggccaac
     5701 agttcctgat taaccacaaa ccgttctact ttactggctt tggtcgtcat gaagatgcgg
     5761 acttacgtgg caaaggattc gataacgtgc tgatggtgca cgaccacgca ttaatggact
     5821 ggattggggc caactcctac cgtacctcgc attaccctta cgctgaagag atgctcgact
     5881 gggcagatga acatggcatc gtggtgattg atgaaactgc tgctgtcggc tttaacctct
     5941 ctttaggcat tggtttcgaa gcgggcaaca agccgaaaga actgtacagc gaagaggcag
     6001 tcaacgggga aactcagcaa gcgcacttac aggcgattaa agagctgata gcgcgtgaca
     6061 aaaaccaccc aagcgtggtg atgtggagta ttgccaacga accggatacc cgtccgcaag
     6121 tgcacgggaa tatttcgcca ctggcggaag caacgcgtaa actcgacccg acgcgtccga
     6181 tcacctgcgt caatgtaatg ttctgcgacg ctcacaccga taccatcagc gatctctttg
     6241 atgtgctgtg cctgaaccgt tattacggat ggtatgtcca aagcggcgat ttggaaacgg
     6301 cagagaaggt actggaaaaa gaacttctgg cctggcagga gaaactgcat cagccgatta
     6361 tcatcaccga atacggcgtg gatacgttag ccgggctgca ctcaatgtac accgacatgt
     6421 ggagtgaaga gtatcagtgt gcatggctgg atatgtatca ccgcgtcttt gatcgcgtca
     6481 gcgccgtcgt cggtgaacag gtatggaatt tcgccgattt tgcgacctcg caaggcatat
     6541 tgcgcgttgg cggtaacaag aaagggatct tcactcgcga ccgcaaaccg aagtcggcgg
     6601 cttttctgct gcaaaaacgc tggactggca tgaacttcgg tgaaaaaccg cagcagggag
     6661 gcaaacaatg aatcaactct cctggcgcac catggtcggc tacagcctcg ggtgacgtca
     6721 agctgacctg catctagccc gcctaatgag cgggcttttt tttagatgca gccaagcttg
     6781 catgcctgca ggtcgactct agaactagtg gatcccccgg gctgcaggcg taccagcttt
     6841 ccctatagtg agtcgtatta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6901 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6961 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7021 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7081 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgagacgg gcaacagctg
     7141 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
     7201 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcttc
     7261 ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat
     7321 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
     7381 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
     7441 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
     7501 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
     7561 tgcgaccaga tgctccacgc ccagtcgcgt accgtcttca tgggagaaaa taatactgtt
     7621 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
     7681 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacccgttg
     7741 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
     7801 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
     7861 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
     7921 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
     7981 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
     8041 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     8101 gagtcgacgg tatcgataag cttggggttt gtgtttccat attgttcatc tcccattgat
     8161 cgtattaaga aagtatgatg gtgatgtcgc agccttccgc tttcgcttca cggaaaacct
     8221 gaagcacact ctcggcgcca ttttcagtca gctgcttgct ttgttcaaac tgcctccatt
     8281 ccaaaacgag cgggtactcc acccatccgg tcagacaatc ccataaagcg tccaggtttt
     8341 caccgtagta ttccggaagg gcaagctcct ttttcaatgt ctggtggagg tcgctgatac
     8401 ttctgatttg ttccccgtta atgactgctt ttttcatgtg cggctccttt cttatgacgg
     8461 atccccgggg aattcggagg tcgtggactt gccgataccc cccttaccgt agaacgcgat
     8521 ctgacgcaga gctgccatct tgcttccttt gttgtttaag ctatttcggt tgttcggaca
     8581 catgaatgcg caacagcatg cgcgcccagg gctgatcgtg caaaagtcgt gccagccgtc
     8641 tgaaataaaa ctactcggct ttctttcaga tagttagatc tggaaattga ccagatggtt
     8701 gagcgaacaa ctcgtgtcgc taagccgaca agggctacgg acagtgtcgt atgggcggtt
     8761 gtcacgcgtc agaaatcgtc aacgaattgc cgccggcgtg gaagcccttg tgaccgggcg
     8821 acgacacgcc gagtggccgc tggagaggcg agacgctgta cgggctgctc tgggtagcgg
     8881 cttctttttg ggccttcctc gcgatcggga acgacagaat ttctgccgca tatgcttagc
     8941 tgctgtgaac gcgagtgcct tccgccgcgg gggaacaaaa gagagcggcc caaaaaaccg
     9001 ggcggctgat gtcgcaatct cgctccagga gccaccggac ccgcctgctc gagggggggc
     9061 ccgtttctca aagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     9121 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     9181 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     9241 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     9301 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     9361 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     9421 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     9481 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     9541 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     9601 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     9661 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     9721 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     9781 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     9841 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     9901 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     9961 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
    10021 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
    10081 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
    10141 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
    10201 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
    10261 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
    10321 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
    10381 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
    10441 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
    10501 tcctttttca atcaggtggc acttttcggg gaaatgtgcg cgcccgcgtt cctgctggcg
    10561 ctgggcctgt ttctggcgct ggacttcccg ctgttccgtc agcagctttt cgcccacggc
    10621 cttgatgatc gcggcggcct tggcctgcat atcccgattc aacggcccca gggcgtccag
    10681 aacgggcttc aggcgctccc gaaggt
//
