LOCUS       V001281                14247 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V001281
VERSION     V001281
KEYWORDS    pHR-SFFV-KRAB-dCas9-P2A-mCherry
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14247)
  AUTHORS   Gilbert LA, Horlbeck MA, Adamson B, Villalta JE, Chen Y, Whitehead
            EH, Guimaraes C, Panning B, Ploegh HL, Bassik MC, Qi LS, Kampmann M,
            Weissman JS
  TITLE     Genome-Scale CRISPR-Mediated Control of Gene Repression and
            Activation.
  JOURNAL   Cell. 2014 Oct 23;159(3):647-61. doi: 10.1016/j.cell.2014.09.029.
            Epub 2014 Oct 9.
   PUBMED   25307932
REFERENCE   2  (bases 1 to 14247)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 14247)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi:
            "10.1016/j.cell.2014.09.029"; journalName: "Cell"; date:
            "2014-10-23- 23"; volume: "159"; issue: "3"; pages: "647-61"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..17)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     LTR             108..341
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(366..384)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(674..693)
                     /label="pBRrevBam"
                     /note="pBR322 vectors, tet region, downstream of BamHI,
                     reverse primer"
     primer_bind     complement(907..926)
                     /label="pRS-marker"
                     /note="pRS vectors, use to sequence yeast selectable
                     marker"
     primer_bind     1026..1048
                     /label="pGEX 3'"
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1086..1104)
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     promoter        1172..1276
                     /label="AmpR promoter"
     CDS             1277..2134
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2308..2896
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     3050..3067
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     promoter        3142..3471
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     intron          4605..4670
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4800..4820
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    5245..5379
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             5547..6180
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6227..6352
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6849..7082
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             7267..7311
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             7460..7501
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    7596..7713
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        7866..8273
                     /label="SFFV promoter"
                     /note="spleen focus-forming virus long terminal repeat
                     (LTR) promoter"
     regulatory      8348..8357
                     /label="Kozak sequence"
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8384..8569
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional
                     repression domain from the human zinc finger protein ZNF10
                     (Margolin et al., 1994)"
                     /label="KRAB"
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEP"
     CDS             8591..12694
                     /label="dCas9"
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             12698..12724
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label="HA"
                     /translation="YPYDVPDYA"
     CDS             12743..12763
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             12770..12790
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label="SV40 NLS"
                     /translation="PKKKRKV"
     CDS             12830..12886
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /label="P2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             12887..13594
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     misc_feature    13657..14245
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
ORIGIN
        1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
       61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
      121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
      181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
      241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
      301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
      361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
      421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
      481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
      541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
      601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
      661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
      721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
      781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
      841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
      901 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
      961 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     1021 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     1081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     1141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     1201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1261 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1321 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1381 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1441 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1501 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1561 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1621 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1681 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1741 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1801 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1861 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1921 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1981 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     2041 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     2101 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     2161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     2221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     2461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     3001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     3061 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     3121 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
     3481 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
     3541 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
     3601 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
     3661 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
     3721 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
     3781 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
     3841 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
     3901 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
     3961 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
     4021 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
     4081 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
     4141 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
     4201 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
     4261 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
     4321 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
     4381 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
     4441 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
     4501 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
     4561 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
     4621 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
     4681 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
     4741 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
     4801 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
     4861 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
     4921 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
     4981 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
     5041 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
     5101 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
     5161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
     5221 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
     5281 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     5341 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
     5401 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
     5461 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
     5521 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
     5581 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     5641 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     5701 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     5761 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     5821 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     5881 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     5941 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     6001 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6061 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6121 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6181 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     6241 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     6301 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     6361 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     6421 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     6481 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     6541 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     6601 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     6661 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
     6721 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
     6781 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
     6841 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
     6901 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
     6961 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
     7021 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
     7081 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
     7141 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
     7201 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
     7261 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
     7321 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
     7381 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
     7441 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
     7501 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
     7561 cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     7621 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     7681 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     7741 ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa taaaagattt
     7801 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     7861 ctgcagtaac gccattttgc aaggcatgga aaaataccaa accaagaata gagaagttca
     7921 gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat ctgcggtgag
     7981 cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg gccccggccc
     8041 gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc ttaagaccca
     8101 tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat ttgaattaac
     8161 caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc tataaaagag
     8221 ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc gggggggatc
     8281 tggagctctc gagaattctc acgcgtctgc aggatatcaa gcttgcggta ccgcgggccc
     8341 gggatccgcc accatggatg ctaagtcact aactgcctgg tcccggacac tggtgacctt
     8401 caaggatgta tttgtggact tcaccaggga ggagtggaag ctgctggaca ctgctcagca
     8461 gatcgtgtac agaaatgtga tgctggagaa ctataagaac ctggtttcct tgggttatca
     8521 gcttactaag ccagatgtga tcctccggtt ggagaaggga gaagagcccg gcggttccgg
     8581 cggagggtcg atggacaaga agtacagcat cggcctggcc atcggcacca actctgtggg
     8641 ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg tgctgggcaa
     8701 caccgaccgg cacagcatca agaagaacct gatcggcgcc ctgctgttcg acagcggaga
     8761 aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca gacggaagaa
     8821 ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg acgacagctt
     8881 cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg agcggcaccc
     8941 catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca ccatctacca
     9001 cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga tctatctggc
     9061 cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc tgaaccccga
     9121 caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc agctgttcga
     9181 ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg ccagactgag
     9241 caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga agaatggcct
     9301 gttcggcaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga gcaacttcga
     9361 cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg acctggacaa
     9421 cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca agaacctgtc
     9481 cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca aggcccccct
     9541 gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc tgctgaaagc
     9601 tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc agagcaagaa
     9661 cggctacgcc ggctacatcg atggcggagc cagccaggaa gagttctaca agttcatcaa
     9721 gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga acagagagga
     9781 cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga tccacctggg
     9841 agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga aggacaaccg
     9901 ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc ctctggccag
     9961 gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca ccccctggaa
    10021 cttcgaggaa gtggtggaca agggcgccag cgcccagagc ttcatcgagc ggatgaccaa
    10081 cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc tgtacgagta
    10141 cttcaccgtg tacaacgagc tgaccaaagt gaaatacgtg accgagggaa tgagaaagcc
    10201 cgccttcctg agcggcgagc agaaaaaagc catcgtggac ctgctgttca agaccaaccg
    10261 gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt gcttcgactc
    10321 cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat accacgatct
    10381 gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg acattctgga
    10441 agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg aacggctgaa
    10501 aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc ggagatacac
    10561 cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc agtccggcaa
    10621 gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca tgcagctgat
    10681 ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt ccggccaggg
    10741 cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta agaagggcat
    10801 cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc acaagcccga
    10861 gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac agaagaacag
    10921 ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc agatcctgaa
    10981 agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt actacctgca
    11041 gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt ccgactacga
    11101 tgtggacgct atcgtgcctc agagctttct gaaggacgac tccatcgata acaaagtgct
    11161 gactcggagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag aggtcgtgaa
    11221 gaagatgaag aactactggc gccagctgct gaatgccaag ctgattaccc agaggaagtt
    11281 cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg ccggcttcat
    11341 caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga tcctggactc
    11401 ccggatgaac actaagtacg acgagaacga caaactgatc cgggaagtga aagtgatcac
    11461 cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca aagtgcgcga
    11521 gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg gaaccgccct
    11581 gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca aggtgtacga
    11641 cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg ccaagtactt
    11701 cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca acggcgagat
    11761 ccggaagcgg cctctgatcg agacaaacgg cgaaacaggc gagatcgtgt gggataaggg
    11821 ccgggacttt gccaccgtgc ggaaagtgct gtctatgccc caagtgaata tcgtgaaaaa
    11881 gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga ggaacagcga
    11941 caagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct tcgacagccc
    12001 caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt ccaagaaact
    12061 gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct tcgagaagaa
    12121 tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc tgatcatcaa
    12181 gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc tggcctctgc
    12241 cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga acttcctgta
    12301 cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc agaaacagct
    12361 gtttgtggaa cagcacaaac actacctgga cgagatcatc gagcagatca gcgagttctc
    12421 caagagagtg atcctggccg acgctaatct ggacaaggtg ctgagcgcct acaacaagca
    12481 cagagacaag cctatcagag agcaggccga gaatatcatc cacctgttta ccctgaccaa
    12541 tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga agaggtacac
    12601 cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg gcctgtacga
    12661 gacacggatc gacctgtctc agctgggagg cgacgcctat ccctatgacg tgcccgatta
    12721 tgccagcctg ggcagcggct cccccaagaa aaaacgcaag gtggaagatc ctaagaaaaa
    12781 gcggaaagtg gacggcattg gtagtgggag caacggcagc agcggatccg ctactaactt
    12841 cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctatgg tgagcaaggg
    12901 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
    12961 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
    13021 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
    13081 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
    13141 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
    13201 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
    13261 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
    13321 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
    13381 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
    13441 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
    13501 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
    13561 ccactccacc ggcggcatgg acgagctgta caagtaagcg gccgcgactc tagagtcgac
    13621 ctgcaggcat gcaagcttga tatcaagctt atcgataatc aacctctgga ttacaaaatt
    13681 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
    13741 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
    13801 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
    13861 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
    13921 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
    13981 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
    14041 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
    14101 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
    14161 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
    14221 atctcccttt gggccgcctc cccgcat
//
