LOCUS       V014052                11438 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014052
VERSION     V014052
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11438)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..11438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        438..542
                     /label="AmpR promoter"
     CDS             543..1400
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1574..2162
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2408..2737
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     intron          3871..3936
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4066..4086
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    4511..4645
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             4814..5447
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5494..5619
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6111..6344
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             6529..6573
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             6722..6763
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    6856..6973
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        7033..7532
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             9368..9808
                     /label="GAL4 DNA binding domain"
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             9845..9994
                     /label="VP64"
                     /note="tetrameric repeat of the minimal activation domain
                     of herpes simplex virus VP16 (Beerli et al., 1998)"
     misc_feature    10113..10701
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             10811..11044
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(11069..11087)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
       61 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
      121 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatcta
      181 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
      241 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
      301 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
      361 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
      421 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      481 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      541 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      601 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      661 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      721 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      781 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      841 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
      901 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
      961 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     1021 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     1081 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     1141 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     1201 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     1261 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     1321 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     1381 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     1441 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     1501 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     1561 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     1621 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     1681 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     1741 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     1801 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     1861 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     1921 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     1981 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     2041 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     2101 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     2161 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     2221 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     2281 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     2341 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     2401 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     2461 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     2521 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     2581 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     2641 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     2701 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt
     2761 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2821 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2881 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     2941 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3001 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc
     3061 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     3121 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     3181 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
     3241 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     3301 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
     3361 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     3421 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
     3481 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     3541 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc
     3601 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
     3661 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg
     3721 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt
     3781 atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     3841 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     3901 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     3961 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     4021 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     4081 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     4141 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     4201 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
     4261 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
     4321 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
     4381 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     4441 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     4501 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4561 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4621 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     4681 acttcccacc ccatacccta ttaccactgc caattaccta gtggtttcat ttactctaaa
     4741 cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta
     4801 caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg
     4861 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     4921 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     4981 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     5041 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     5101 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     5161 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     5221 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     5281 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5341 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     5401 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     5461 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     5521 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     5581 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     5641 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     5701 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     5761 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     5821 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     5881 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     5941 accaccgcac agcaagcggc cggtgatctt cagacctgga cgatatatat gagggacaat
     6001 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     6061 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     6121 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     6181 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     6241 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     6301 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     6361 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     6421 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     6481 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     6541 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     6601 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     6661 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     6721 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     6781 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggtc gccaaatggc
     6841 agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag
     6901 aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa
     6961 aattcaaaat tttcgggttt attacaggga cagcagagat ccagtttgga tcgataagct
     7021 tgatatcgaa ttgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     7081 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     7141 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     7201 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     7261 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     7321 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     7381 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     7441 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     7501 tgttctcctc ttcctcatct ccgggccttt cgaattctca cgcgtcaagt ggagcaaggc
     7561 aggtggacag tggatcatgg cgctccctgt caccgcactg cttcttccgc tggcactgct
     7621 gctgcacgct gcacggcctg agcaaaaact tatctctgaa gaggacctcg aggtgcagct
     7681 ggtggagagc ggcggcggcc tggtgcagcc cggcggcagc ctgagactga gctgcgccgc
     7741 cagcggcttc accttcagca actactacat gagctgggtg agacaggccc ccggcaaggg
     7801 cctggagtgg gtggccacca tcaaccccag cggcaccaga acctactacc ccgacagcgt
     7861 gaagggcaga ttcaccatca gcagagacaa cgccaagaac agcctgtacc tgcagatgaa
     7921 cagcctgaga gccgaggaca ccgccgtgta ctactgcgcc agactgtaca acaactacgc
     7981 cttcgactac tggggccagg gcaccctggt gaccgtgagc agcggcggcg gcggcagcgg
     8041 cggcggcggc agcggcggcg gcggcagcga catccagatg acccagagcc ccagcagcct
     8101 gagcgccagc gtgggcgaca gagtgaccat cacctgcaga gccagcgaga gcatcagcac
     8161 cctgctgcac tggtatcagc agaagcccgg caaggccccc aagctgctga tctacggcac
     8221 cagcaacctg gagagcggcg tgcccagcag attcagcggc agcggcagcg gcaccgactt
     8281 caccctgacc atcagcagcc tgcagcccga ggacttcgcc acctactact gccagcagag
     8341 ctggaacgac ccccccacct tcggcggcgg caccaaggtg gagatcaaga tcctggacta
     8401 cagcttcaca ggtggcgctg ggcgcgacat tcccccaccg cagattgagg aggcctgtga
     8461 gctgcctgag tgccaggtgg atgcaggcaa taaggtctgc aacctgcagt gtaataatca
     8521 cgcatgtggc tgggatggtg gcgactgctc cctcaacttc aatgacccct ggaagaactg
     8581 cacgcagtct ctacagtgct ggaagtattt tagcgacggc cactgtgaca gccagtgcaa
     8641 ctcggccggc tgcctctttg atggcttcga ctgccagctc accgagggac agtgcaaccc
     8701 cctgtatgac cagtactgca aggaccactt cagtgatggc cactgcgacc agggctgtaa
     8761 cagtgccgaa tgtgagtggg atggcctaga ctgtgctgag catgtacccg agcggctggc
     8821 agccggcacc ctggtgctgg tggtgctgct tccacccgac cagctacgga acaactcctt
     8881 ccactttctg cgggagctca gccacgtgct gcacaccaac gtggtcttca agcgtgatgc
     8941 gcaaggccag cagatgatct tcccgtacta tggccacgag gaagagctgc gcaagcaccc
     9001 aatcaagcgc tctacagtgg gttgggccac ctcttcactg cttcctggta ccagtggtgg
     9061 gcgccagcgc agggagctgg accccatgga catccgtggc tccattgtct acctggagat
     9121 cgacaaccgg caatgtgtgc agtcatcctc gcagtgcttc cagagtgcca ccgatgtggc
     9181 tgccttccta ggtgctcttg cgtcacttgg cagcctcaat attccttaca agattgaggc
     9241 cgtgaagagt gagccggtgg agcctccgct gccctcgcag ctgcacctca tgtacgtggc
     9301 agcggccgcc ttcgtgctcc tgttctttgt gggctgtggg gtgctgctgt cccgcaagcg
     9361 ccggcggatg aagctgctga gcagcatcga gcaggcctgt gacatctgcc ggctgaagaa
     9421 actgaagtgc agcaaagaaa agcccaagtg cgccaagtgc ctgaagaaca actgggagtg
     9481 ccggtacagc cccaagacca agagaagccc cctgaccaga gcccacctga ccgaggtgga
     9541 aagccggctg gaaagactgg aacagctgtt tctgctgatc ttcccacgcg aggacctgga
     9601 catgatcctg aagatggaca gcctgcagga catcaaggcc ctgctgaccg gcctgttcgt
     9661 gcaggacaac gtgaacaagg acgccgtgac cgacagactg gccagcgtgg aaaccgacat
     9721 gcccctgacc ctgcggcagc acagaatcag cgccaccagc agcagcgagg aaagcagcaa
     9781 caagggccag cggcagctga cagtgtctgc tgctgcaggc ggaagcggag gctctggcgg
     9841 atctgatgcc ctggacgact tcgacctgga tatgctgggc agcgacgccc tggatgattt
     9901 tgatctggac atgctgggat ctgacgctct ggacgatttc gatctcgaca tgttgggatc
     9961 agatgcactg gatgactttg acctggacat gctcggatca tgagatcctt gacttgcggc
    10021 cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga gtcgacctgc
    10081 aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
    10141 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
    10201 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
    10261 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
    10321 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
    10381 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
    10441 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
    10501 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
    10561 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
    10621 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
    10681 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa ttcgagctcg
    10741 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
    10801 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
    10861 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
    10921 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
    10981 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    11041 agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga
    11101 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt
    11161 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag
    11221 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg
    11281 tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg
    11341 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
    11401 gggaagatcg ggctcgccac ttcgggctca tgagcgct
//
