LOCUS       V014040                14702 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014040
VERSION     V014040
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 14702)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..14702
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             61..294
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(319..337)
                     /label="SP6 promoter"
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        1126..1230
                     /label="AmpR promoter"
     CDS             1231..2088
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2262..2850
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        3096..3425
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     intron          4559..4624
                     /label="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4754..4774
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    5199..5333
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     LTR             5502..6135
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    6182..6307
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    6799..7032
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             7217..7261
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             7410..7451
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    7544..7661
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     CDS             8062..8127
                     /label="3xFLAG"
                     /note="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
     CDS             9580..9636
                     /label="P2A"
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
     CDS             9739..10395
                     /gene="IL12A"
                     /label="Interleukin-12 subunit alpha"
                     /note="Interleukin-12 subunit alpha from Homo sapiens.
                     Accession#: P29459"
     CDS             10405..10458
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             10459..11442
                     /gene="IL12B"
                     /label="Interleukin-12 subunit beta"
                     /note="Interleukin-12 subunit beta from Homo sapiens.
                     Accession#: P29460"
     misc_feature    11468..12041
                     /label="IRES"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             12060..12767
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     promoter        12855..13354
                     /label="PGK promoter"
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             13375..14073
                     /label="TagBFP"
                     /note="monomeric blue fluorescent protein"
     misc_feature    14112..14700
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
ORIGIN
        1 cgataccgtc gaccaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
       61 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
      121 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      181 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      241 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct
      301 agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt
      361 tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc
      421 tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt
      481 tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc
      541 aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac
      601 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg
      661 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt
      721 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct
      781 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg
      841 tcgaatggtg cactctcagt acaatctagc tctgatgccg catagttaag ccagccccga
      901 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
      961 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     1021 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     1081 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     1141 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     1201 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     1261 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     1321 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     1381 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     1441 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     1501 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     1561 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     1621 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     1681 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     1741 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     1801 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     1861 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     1921 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     1981 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     2041 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     2101 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     2161 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
     2221 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     2281 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     2341 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     2401 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     2461 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     2521 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
     2581 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     2641 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     2701 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     2761 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     2821 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     2881 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     2941 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
     3001 cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc
     3061 gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     3121 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     3181 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     3241 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3301 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3361 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3421 gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc
     3481 gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt
     3541 gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta
     3601 gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg
     3661 cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt
     3721 attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc
     3781 gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag
     3841 tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata
     3901 ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct
     3961 tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata
     4021 cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct
     4081 aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa
     4141 tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc
     4201 ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac
     4261 ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc
     4321 atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc
     4381 caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg
     4441 attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc
     4501 ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt
     4561 aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt
     4621 ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga
     4681 aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca
     4741 acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga
     4801 attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat
     4861 ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt
     4921 aacctttata agtaggcata acagttataa tcataacata ctgttttttc ttactccaca
     4981 caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt
     5041 aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa
     5101 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     5161 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     5221 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     5281 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga
     5341 taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca
     5401 attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt ttcattttaa
     5461 agaaattgta tttgttaaat atgtactaca aacttagtag ttggaagggc taattcactc
     5521 ccaaagaaga caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga
     5581 ttagcagaac tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta
     5641 caagctagta ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag
     5701 cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg
     5761 gaggtttgac agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt
     5821 caagaactgc tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag
     5881 gcgtggcctg ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc
     5941 tttttgcctg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6001 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6061 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6121 ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga
     6181 gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg
     6241 actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga
     6301 gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc
     6361 agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg
     6421 attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca
     6481 gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc
     6541 aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa
     6601 gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg gtgatcttca
     6661 gacctggacg atatatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     6721 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     6781 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     6841 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     6901 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     6961 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     7021 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     7081 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     7141 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     7201 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     7261 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     7321 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     7381 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     7441 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     7501 gaacggatct cgacggtcgc caaatggcag tattcatcca caattttaaa agaaaagggg
     7561 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     7621 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     7681 gcagagatcc agtttggatc gataagcttg atatcgaatt cggagcactg tcctccgaac
     7741 gtcggagcac tgtcctccga acgtcggagc actgtcctcc gaacgtcgga gcactgtcct
     7801 ccgaacggag catgtcctcc gaacgtcgga gcactgtcct ccgaacgact agttaggcgt
     7861 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     7921 cgccatccac gctgttttga cctccataga agacaccggg accgatccag cctctcgaca
     7981 ttcgttggat ccggatccat gctcaggctg ctcttggctc tcaacttatt cccttcaatt
     8041 caagtaacag gagggtcttc ggactacaag gatcatgacg gagactataa ggatcacgat
     8101 attgattaca aagatgacga cgacaaacag gtgcagctgc agcagagcgg cgccgagctg
     8161 gtgagacccg gcgccagcgt gaagctgagc tgcaaggcca gcggctacac cttcaccgac
     8221 tacgagatgc actgggtgaa gcagaccccc gtgcacggcc tgaagtggat cggcgccctg
     8281 gaccccaaga ccggcgacac cgcctacagc cagaagttca agggcaaggc caccctgacc
     8341 gccgacaaga gcagcagcac cgcctacatg gagctgagaa gcctgaccag cgaggacagc
     8401 gccgtgtact actgcaccag attctacagc tacacctact ggggccaggg caccctggtg
     8461 accgtgagcg gcggcggcgg cagcggcggc ggcggcagcg gcggcggcgg cagcgacgtg
     8521 gtgatgaccc agacccccct gagcctgccc gtgagcctgg gcgaccaggc cagcatcagc
     8581 tgcagaagca gccagagcct ggtgcacagc aacggcaaca cctacctgca ctggtacctg
     8641 cagaagcccg gccagagccc caagctgctg atctacaagg tgagcaacag attcagcggc
     8701 gtgcccgaca gattcagcgg cagcggcagc ggcaccgact tcaccctgaa gatcagcaga
     8761 gtggaggccg aggacctggg cgtgtacttc tgcagccaga acacccacgt gccccccacc
     8821 ttcggcagcg gcaccaagct ggagatcaag agaggtacca ccacaacgcc cgctcctcgg
     8881 ccaccgacgc cagcgccaac tattgcgagt cagcctctca gtctgcgacc tgaggcttgt
     8941 cgaccagcag ccggaggcgc agtgcacacg agggggctgg acttcgcctg tgatagaaga
     9001 cctccttcta agcccttttg ggtgctggtg gtggttggtg gagtcctggc ttgctatagc
     9061 ttgctagtaa cagtggcctt tattattttc tgggtgagga aacggggcag aaagaaactc
     9121 ctgtatatat tcaaacaacc atttatgaga ccagtacaaa ctactcaaga ggaagatggc
     9181 tgtagctgcc gatttccaga agaagaagaa ggaggatgtg aactggctag cctgagagtg
     9241 aagttcagca ggagcgcaga cgcccccgcg taccagcagg gccagaacca gctctataac
     9301 gagctcaatc taggacgaag agaggagtac gatgttttgg acaagagacg tggccgggac
     9361 cctgagatgg ggggaaagcc gcagagaagg aagaaccctc aggaaggcct gtacaatgaa
     9421 ctgcagaaag ataagatggc ggaggcctac agtgagattg ggatgaaagg cgagcgccgg
     9481 aggggcaagg ggcacgatgg cctttaccag ggtctcagta cagccaccaa ggacacctac
     9541 gacgcccttc acatgcaggc cctgccccct cgcgctagcg ccacgaactt ctctctgtta
     9601 aagcaagcag gcgacgtgga agaaaacccc ggtcccatgt ggccccctgg gtcagcctcc
     9661 cagccaccgc cctcacctgc cgcggccaca ggtctgcatc cagcggctcg ccctgtgtcc
     9721 ctgcagtgcc ggctcagcat gtgtccagcg cgcagcctcc tccttgtggc taccctggtc
     9781 ctcctggacc acctcagttt ggccagaaac ctccccgtgg ccactccaga cccaggaatg
     9841 ttcccatgcc ttcaccactc ccaaaacctg ctgagggccg tcagcaacat gctccagaag
     9901 gccagacaaa ctctagaatt ttacccttgc acttctgaag agattgatca tgaagatatc
     9961 acaaaagata aaaccagcac agtggaggcc tgtttaccat tggaattaac caagaatgag
    10021 agttgcctaa attccagaga gacctctttc ataactaatg ggagttgcct ggcctccaga
    10081 aagacctctt ttatgatggc cctgtgcctt agtagtattt atgaagactt gaagatgtac
    10141 caggtggagt tcaagaccat gaatgcaaag cttctgatgg accctaagag gcagatcttt
    10201 ctagatcaaa acatgctggc agttattgat gagctgatgc aggccctgaa tttcaacagt
    10261 gagactgtgc cacaaaaatc ctcccttgaa gaaccggatt tttataaaac taaaatcaag
    10321 ctctgcatac ttcttcatgc tttcagaatt cgggcagtga ctattgatag agtgatgagc
    10381 tatctgaatg cttccggctc cggcgagggc aggggaagtc tactaacatg cggggacgtg
    10441 gaggaaaatc ccggcccaat gtgtcaccag cagttggtca tctcttggtt ttccctggtt
    10501 tttctggcat ctcccctcgt ggccatatgg gaactgaaga aagatgttta tgtcgtagaa
    10561 ttggattggt atccggatgc ccctggagaa atggtggtcc tcacctgtga cacccctgaa
    10621 gaagatggta tcacctggac cttggaccag agcagtgagg tcttaggctc tggcaaaacc
    10681 ctgaccatcc aagtcaaaga gtttggagat gctggccagt acacctgtca caaaggaggc
    10741 gaggttctaa gccattcgct cctgctgctt cacaaaaagg aagatggaat ttggtccact
    10801 gatattttaa aggaccagaa agaacccaaa aataagacct ttctaagatg cgaggccaag
    10861 aattattctg gacgtttcac ctgctggtgg ctgacgacaa tcagtactga tttgacattc
    10921 agtgtcaaaa gcagcagagg ctcttctgac ccccaagggg tgacgtgcgg agctgctaca
    10981 ctctctgcag agagagtcag aggggacaac aaggagtatg agtactcagt ggagtgccag
    11041 gaggacagtg cctgcccagc tgctgaggag agtctgccca ttgaggtcat ggtggatgcc
    11101 gttcacaagc tcaagtatga aaactacacc agcagcttct tcatcaggga catcatcaaa
    11161 cctgacccac ccaagaactt gcagctgaag ccattaaaga attctcggca ggtggaggtc
    11221 agctgggagt accctgacac ctggagtact ccacattcct acttctccct gacattctgc
    11281 gttcaggtcc agggcaagag caagagagaa aagaaagata gagtcttcac ggacaagacc
    11341 tcagccacgg tcatctgccg caaaaatgcc agcattagcg tgcgggccca ggaccgctac
    11401 tatagctcat cttggagcga atgggcatct gtgccctgca gttagtaagg atccttgact
    11461 tgcggccccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
    11521 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
    11581 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
    11641 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
    11701 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
    11761 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
    11821 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
    11881 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
    11941 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta ggccccccga
    12001 accacgggga cgtggttttc ctttgaaaaa cacgatgata aatgagcgct ggcgggtcca
    12061 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
    12121 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
    12181 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
    12241 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
    12301 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
    12361 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
    12421 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
    12481 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
    12541 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
    12601 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
    12661 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
    12721 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtag ggccgcaact
    12781 cccacctgca acatgcgtga ctgactgagg ccgcgactct agagtcgacc tgcacgaggt
    12841 taacgaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
    12901 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
    12961 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
    13021 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
    13081 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
    13141 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
    13201 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
    13261 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
    13321 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgagc
    13381 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat
    13441 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga
    13501 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc
    13561 ctctacggca gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag
    13621 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg
    13681 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga
    13741 ggggtgaact tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc
    13801 ttcaccgaga cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg
    13861 aagctcgtgg gcgggagcca tctgatcgca aacatcaaga ccacatatag atccaagaaa
    13921 cccgctaaga acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc
    13981 aaggaggcca acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc
    14041 gacctcccta gcaaactggg gcacaagctt aattaatgca ggcatgcaag cttgatatca
    14101 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
    14161 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
    14221 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
    14281 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
    14341 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
    14401 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
    14461 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
    14521 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
    14581 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
    14641 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
    14701 at
//
