LOCUS       40924_24852        5238 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHQRep(6X), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma JZ, Ma YL, Huang F.
  TITLE     An efficient plant transient expression vector system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Ma JZ, Ma YL, Huang F.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2014) School of Life Science, LanZhou University 
            of Technology, Qili River Street, Lanzhou, Gansu 730050, China
REFERENCE   3  (bases 1 to 5238)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5238)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JAN-2014) School of Life Science, LanZhou University of 
            Technology, Qili River Street, Lanzhou, Gansu 730050, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(945..1802)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1803..1907)
                     /label=AmpR promoter
     primer_bind     2381..2397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2404..2531
                     /label=contains six repeated upstream GAL4 binding sites
                     /note="contains six repeated upstream GAL4 binding sites"
     regulatory      2544..2656
                     /label=partial 35S promoter
                     /note="partial 35S promoter"
                     /regulatory_class="promoter"
     promoter        2599..2645
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     intron          2679..2868
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             2863..4686
                     /label=GUS
                     /note="beta-glucuronidase"
     CDS             4693..4710
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      4745..4997
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5019..5035)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5043..5059)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5067..5097)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5112..5133)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     1981 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2041 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2101 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     2161 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     2221 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     2281 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     2341 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     2401 ttccggagga ctgtcctccg agcggaggac tgtcctccga gcggaggact gtcctccggg
     2461 atcctctaga gaattccgga ggactgtcct ccgagcggag gactgtcctc cgagcggagg
     2521 actgtcctcc gggatcctct agaattgatg tgatatctcc actgacgtaa gggatgacgc
     2581 acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga
     2641 gagaacacgg gggactcttg accatggtag atctgagggt aaatttctag tttttctcct
     2701 tcattttctt ggttaggacc cttttctctt tttatttttt tgagctttga tctttcttta
     2761 aactgatcta ttttttaatt gattggttat ggtgtaaata ttacatagct ttaactgata
     2821 atctgattac tttatttcgt gtgtctatga tgatgatgat agttacagaa ccgacgactc
     2881 gtccgtcctg tagaaacccc aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc
     2941 agtctggatc gcgaaaactg tggaattgat cagcgttggt gggaaagcgc gttacaagaa
     3001 agccgggcaa ttgctgtgcc aggcagtttt aacgatcagt tcgccgatgc agatattcgt
     3061 aattatgcgg gcaacgtctg gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc
     3121 cagcgtatcg tgctgcgttt cgatgcggtc actcattacg gcaaagtgtg ggtcaataat
     3181 caggaagtga tggagcatca gggcggctat acgccatttg aagccgatgt cacgccgtat
     3241 gttattgccg ggaaaagtgt acgtatcacc gtttgtgtga acaacgaact gaactggcag
     3301 actatcccgc cgggaatggt gattaccgac gaaaacggca agaaaaagca gtcttacttc
     3361 catgatttct ttaactatgc cggaatccat cgcagcgtaa tgctctacac cacgccgaac
     3421 acctgggtgg acgatatcac cgtggtgacg catgtcgcgc aagactgtaa ccacgcgtct
     3481 gttgactggc aggtggtggc caatggtgat gtcagcgttg aactgcgtga tgcggatcaa
     3541 caggtggttg caactggaca aggcactagc gggactttgc aagtggtgaa tccgcacctc
     3601 tggcaaccgg gtgaaggtta tctctatgaa ctcgaagtca cagccaaaag ccagacagag
     3661 tctgatatct acccgcttcg cgtcggcatc cggtcagtgg cagtgaaggg ccaacagttc
     3721 ctgattaacc acaaaccgtt ctactttact ggctttggtc gtcatgaaga tgcggactta
     3781 cgtggcaaag gattcgataa cgtgctgatg gtgcacgacc acgcattaat ggactggatt
     3841 ggggccaact cctaccgtac ctcgcattac ccttacgctg aagagatgct cgactgggca
     3901 gatgaacatg gcatcgtggt gattgatgaa actgctgctg tcggctttca gctgtcttta
     3961 ggcattggtt tcgaagcggg caacaagccg aaagaactgt acagcgaaga ggcagtcaac
     4021 ggggaaactc agcaagcgca cttacaggcg attaaagagc tgatagcgcg tgacaaaaac
     4081 cacccaagcg tggtgatgtg gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca
     4141 cgggaatatt tcgcgccact ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc
     4201 acctgcgtca atgtaatgtt ctgcgacgct cacaccgata ccatcagcga tctctttgat
     4261 gtgctgtgcc tgaaccgtta ttacggatgg tatgtccaaa gcggcgattt ggaaacggca
     4321 gagaaggtac tggaaaaaga acttctggcc tggcaggaga aactgcatca gccgattatc
     4381 atcaccgaat acggcgtgga tacgttagcc gggctgcact caatgtacac cgacatgtgg
     4441 agtgaagagt atcagtgtgc atggctggat atgtatcacc gcgtctttga tcgcgtcagc
     4501 gccgtcgtcg gtgaacaggt atggaatttc gccgattttg cgacctcgca aggcatattg
     4561 cgcgttggcg gtaacaagaa agggatcttc actcgcgacc gcaaaccgaa gtcggcggct
     4621 tttctgctgc aaaaacgctg gactggcatg aacttcggtg aaaaaccgca gcagggaggc
     4681 aaacaagcta gccaccacca ccaccaccac gtgtgaatta caggtgacca gctcgaattt
     4741 ccccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     4801 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     4861 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     4921 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     4981 atctatgtta ctagatcggg aagcttggcg taatcatggt catagctgtt tcctgtgtga
     5041 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     5101 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     5161 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5221 ggtttgcgta ttgggcgc
//
