LOCUS       40924_24847        5165 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHQRep(3X), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5165)
  AUTHORS   Ma JZ, Ma YL, Huang F.
  TITLE     An efficient plant transient expression vector system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5165)
  AUTHORS   Ma JZ, Ma YL, Huang F.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2014) School of Life Science, LanZhou University 
            of Technology, Qili River Street, Lanzhou, Gansu 730050, China
REFERENCE   3  (bases 1 to 5165)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5165)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-JAN-2014) School of Life Science, LanZhou University of 
            Technology, Qili River Street, Lanzhou, Gansu 730050, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(945..1802)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1803..1907)
                     /label=AmpR promoter
     primer_bind     2381..2397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    2404..2458
                     /note="contains three repeated upstream GAL4-DNA bindng
                     sites"
     regulatory      2471..2583
                     /label=partial 35S promoter
                     /note="partial 35S promoter"
                     /regulatory_class="promoter"
     promoter        2526..2572
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     intron          2606..2795
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             2790..4613
                     /label=GUS
                     /note="beta-glucuronidase"
     CDS             4620..4637
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      4672..4924
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4946..4962)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4970..4986)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4994..5024)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5039..5060)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     1981 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2041 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2101 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     2161 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     2221 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     2281 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     2341 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     2401 ttccggagga ctgtcctccg agcggaggac tgtcctccga gcggaggact gtcctccggg
     2461 atcctctaga attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     2521 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     2581 actcttgacc atggtagatc tgagggtaaa tttctagttt ttctccttca ttttcttggt
     2641 taggaccctt ttctcttttt atttttttga gctttgatct ttctttaaac tgatctattt
     2701 tttaattgat tggttatggt gtaaatatta catagcttta actgataatc tgattacttt
     2761 atttcgtgtg tctatgatga tgatgatagt tacagaaccg acgactcgtc cgtcctgtag
     2821 aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg ggcattcagt ctggatcgcg
     2881 aaaactgtgg aattgatcag cgttggtggg aaagcgcgtt acaagaaagc cgggcaattg
     2941 ctgtgccagg cagttttaac gatcagttcg ccgatgcaga tattcgtaat tatgcgggca
     3001 acgtctggta tcagcgcgaa gtctttatac cgaaaggttg ggcaggccag cgtatcgtgc
     3061 tgcgtttcga tgcggtcact cattacggca aagtgtgggt caataatcag gaagtgatgg
     3121 agcatcaggg cggctatacg ccatttgaag ccgatgtcac gccgtatgtt attgccggga
     3181 aaagtgtacg tatcaccgtt tgtgtgaaca acgaactgaa ctggcagact atcccgccgg
     3241 gaatggtgat taccgacgaa aacggcaaga aaaagcagtc ttacttccat gatttcttta
     3301 actatgccgg aatccatcgc agcgtaatgc tctacaccac gccgaacacc tgggtggacg
     3361 atatcaccgt ggtgacgcat gtcgcgcaag actgtaacca cgcgtctgtt gactggcagg
     3421 tggtggccaa tggtgatgtc agcgttgaac tgcgtgatgc ggatcaacag gtggttgcaa
     3481 ctggacaagg cactagcggg actttgcaag tggtgaatcc gcacctctgg caaccgggtg
     3541 aaggttatct ctatgaactc gaagtcacag ccaaaagcca gacagagtct gatatctacc
     3601 cgcttcgcgt cggcatccgg tcagtggcag tgaagggcca acagttcctg attaaccaca
     3661 aaccgttcta ctttactggc tttggtcgtc atgaagatgc ggacttacgt ggcaaaggat
     3721 tcgataacgt gctgatggtg cacgaccacg cattaatgga ctggattggg gccaactcct
     3781 accgtacctc gcattaccct tacgctgaag agatgctcga ctgggcagat gaacatggca
     3841 tcgtggtgat tgatgaaact gctgctgtcg gctttcagct gtctttaggc attggtttcg
     3901 aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc agtcaacggg gaaactcagc
     3961 aagcgcactt acaggcgatt aaagagctga tagcgcgtga caaaaaccac ccaagcgtgg
     4021 tgatgtggag tattgccaac gaaccggata cccgtccgca aggtgcacgg gaatatttcg
     4081 cgccactggc ggaagcaacg cgtaaactcg acccgacgcg tccgatcacc tgcgtcaatg
     4141 taatgttctg cgacgctcac accgatacca tcagcgatct ctttgatgtg ctgtgcctga
     4201 accgttatta cggatggtat gtccaaagcg gcgatttgga aacggcagag aaggtactgg
     4261 aaaaagaact tctggcctgg caggagaaac tgcatcagcc gattatcatc accgaatacg
     4321 gcgtggatac gttagccggg ctgcactcaa tgtacaccga catgtggagt gaagagtatc
     4381 agtgtgcatg gctggatatg tatcaccgcg tctttgatcg cgtcagcgcc gtcgtcggtg
     4441 aacaggtatg gaatttcgcc gattttgcga cctcgcaagg catattgcgc gttggcggta
     4501 acaagaaagg gatcttcact cgcgaccgca aaccgaagtc ggcggctttt ctgctgcaaa
     4561 aacgctggac tggcatgaac ttcggtgaaa aaccgcagca gggaggcaaa caagctagcc
     4621 accaccacca ccaccacgtg tgaattacag gtgaccagct cgaatttccc cgatcgttca
     4681 aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc
     4741 atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta
     4801 tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa
     4861 aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta
     4921 gatcgggaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     4981 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     5041 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     5101 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     5161 ggcgc
//
