LOCUS       40924_24842        5125 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pHQMini35S-GUS, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Ma JZ, Ma YL, Huang F.
  TITLE     An efficient plant transient expression vector system
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Ma JZ, Ma YL, Huang F.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-JAN-2014) School of Life Science and Engineering, 
            LanZhou University of Technology, No.287 Langongping Road, 
            QiliheDistrict, Lanzhou 730050, China
REFERENCE   3  (bases 1 to 5125)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5125)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-JAN-2014) School of Life Science and Engineering, LanZhou 
            University of Technology, No.287 Langongping Road, QiliheDistrict, 
            Lanzhou 730050, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(183..771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(945..1802)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(1803..1907)
                     /label=AmpR promoter
     primer_bind     2381..2397
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2431..2543
                     /label=mini-35S(-87/+25) promoter
                     /note="mini-35S(-87/+25) promoter"
                     /regulatory_class="promoter"
     promoter        2486..2532
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     intron          2566..2755
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             2750..4573
                     /label=GUS
                     /note="beta-glucuronidase"
     CDS             4580..4597
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      4632..4884
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4906..4922)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4930..4946)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4954..4984)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4999..5020)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
ORIGIN
        1 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
       61 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
      121 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
      181 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
      241 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
      301 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
      361 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
      421 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
      481 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
      541 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
      601 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
      661 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
      721 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
      781 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
      841 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
      901 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
      961 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     1021 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     1081 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     1141 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     1201 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     1261 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     1321 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     1381 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     1441 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     1501 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     1561 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     1621 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     1681 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     1741 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     1801 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     1861 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     1921 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     1981 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
     2041 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
     2101 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
     2161 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
     2221 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     2281 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
     2341 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
     2401 ttcgagctcg gtacccgggg atcctctaga attgatgtga tatctccact gacgtaaggg
     2461 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
     2521 atttggagag aacacggggg actcttgacc atggtagatc tgagggtaaa tttctagttt
     2581 ttctccttca ttttcttggt taggaccctt ttctcttttt atttttttga gctttgatct
     2641 ttctttaaac tgatctattt tttaattgat tggttatggt gtaaatatta catagcttta
     2701 actgataatc tgattacttt atttcgtgtg tctatgatga tgatgatagt tacagaaccg
     2761 acgactcgtc cgtcctgtag aaaccccaac ccgtgaaatc aaaaaactcg acggcctgtg
     2821 ggcattcagt ctggatcgcg aaaactgtgg aattgatcag cgttggtggg aaagcgcgtt
     2881 acaagaaagc cgggcaattg ctgtgccagg cagttttaac gatcagttcg ccgatgcaga
     2941 tattcgtaat tatgcgggca acgtctggta tcagcgcgaa gtctttatac cgaaaggttg
     3001 ggcaggccag cgtatcgtgc tgcgtttcga tgcggtcact cattacggca aagtgtgggt
     3061 caataatcag gaagtgatgg agcatcaggg cggctatacg ccatttgaag ccgatgtcac
     3121 gccgtatgtt attgccggga aaagtgtacg tatcaccgtt tgtgtgaaca acgaactgaa
     3181 ctggcagact atcccgccgg gaatggtgat taccgacgaa aacggcaaga aaaagcagtc
     3241 ttacttccat gatttcttta actatgccgg aatccatcgc agcgtaatgc tctacaccac
     3301 gccgaacacc tgggtggacg atatcaccgt ggtgacgcat gtcgcgcaag actgtaacca
     3361 cgcgtctgtt gactggcagg tggtggccaa tggtgatgtc agcgttgaac tgcgtgatgc
     3421 ggatcaacag gtggttgcaa ctggacaagg cactagcggg actttgcaag tggtgaatcc
     3481 gcacctctgg caaccgggtg aaggttatct ctatgaactc gaagtcacag ccaaaagcca
     3541 gacagagtct gatatctacc cgcttcgcgt cggcatccgg tcagtggcag tgaagggcca
     3601 acagttcctg attaaccaca aaccgttcta ctttactggc tttggtcgtc atgaagatgc
     3661 ggacttacgt ggcaaaggat tcgataacgt gctgatggtg cacgaccacg cattaatgga
     3721 ctggattggg gccaactcct accgtacctc gcattaccct tacgctgaag agatgctcga
     3781 ctgggcagat gaacatggca tcgtggtgat tgatgaaact gctgctgtcg gctttcagct
     3841 gtctttaggc attggtttcg aagcgggcaa caagccgaaa gaactgtaca gcgaagaggc
     3901 agtcaacggg gaaactcagc aagcgcactt acaggcgatt aaagagctga tagcgcgtga
     3961 caaaaaccac ccaagcgtgg tgatgtggag tattgccaac gaaccggata cccgtccgca
     4021 aggtgcacgg gaatatttcg cgccactggc ggaagcaacg cgtaaactcg acccgacgcg
     4081 tccgatcacc tgcgtcaatg taatgttctg cgacgctcac accgatacca tcagcgatct
     4141 ctttgatgtg ctgtgcctga accgttatta cggatggtat gtccaaagcg gcgatttgga
     4201 aacggcagag aaggtactgg aaaaagaact tctggcctgg caggagaaac tgcatcagcc
     4261 gattatcatc accgaatacg gcgtggatac gttagccggg ctgcactcaa tgtacaccga
     4321 catgtggagt gaagagtatc agtgtgcatg gctggatatg tatcaccgcg tctttgatcg
     4381 cgtcagcgcc gtcgtcggtg aacaggtatg gaatttcgcc gattttgcga cctcgcaagg
     4441 catattgcgc gttggcggta acaagaaagg gatcttcact cgcgaccgca aaccgaagtc
     4501 ggcggctttt ctgctgcaaa aacgctggac tggcatgaac ttcggtgaaa aaccgcagca
     4561 gggaggcaaa caagctagcc accaccacca ccaccacgtg tgaattacag gtgaccagct
     4621 cgaatttccc cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg
     4681 ccggtcttgc gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta
     4741 acatgtaatg catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat
     4801 acatttaata cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg
     4861 cggtgtcatc tatgttacta gatcgggaag cttggcgtaa tcatggtcat agctgtttcc
     4921 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     4981 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     5041 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     5101 gagaggcggt ttgcgtattg ggcgc
//
