LOCUS       V005524                 4748 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005524
VERSION     V005524
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Jakobsen OM, Benichou A, Flickinger MC, Valla S, Ellingsen TE,
            Brautaset T.
  TITLE     Upregulated transcription of plasmid and chromosomal ribulose
            monophosphate pathway genes is critical for methanol assimilation
            rate and methanol tolerance in the methylotrophic bacterium Bacillus
            methanolicus
  JOURNAL   J. Bacteriol. 188 (8), 3063-3072 (2006)
   PUBMED   16585766
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Jakobsen OM, Benichou A, Flickinger MC, Valla S, Ellingsen TE,
            Brautaset T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2005) Department of Biotechnology, Norwegian
            University of Science and Technology, Sem Saelands vei 6/8,
            Trondheim 7491, Norway
REFERENCE   3  (bases 1 to 4748)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4748)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2006"; volume: "188"; issue: "8"; pages:
            "3063-3072"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-NOV-2005) Department of Biotechnology, Norwegian University of
            Science and Technology, Sem Saelands vei 6/8, Trondheim 7491,
            Norway"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      565..1153
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    1441..1462
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1477..1507
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1515..1531
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1539..1555
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(1604..1620)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(1832..2479)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(join(4313..4748,1..296))
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
ORIGIN
        1 tatattttat aggattggtt tttaggaaat ttaaactgca atatatcctt gtttaaaact
       61 tggaaattat cgtgatcaac aagtttattt tctgtagttt tgcataattt atggtctatt
      121 tcaatggcag ttacgaaatt acacctcttt actaattcaa gggtaaaatg gccttttcct
      181 gagccgattt caaagatatt atcatgttca tttaatctta tatttgtcat tattttatct
      241 atattatgtt ttgaagtaat aaagttttga ctgtgtttta tatttttctc gttcattata
      301 accctcttta atttggttat atgaattttg cttattaacg attcattata accacttatt
      361 ttttgtttgg ttgataatga actgtgctga ttacaaaaat actaaaaatg cccatatttt
      421 ttcctcctta taaaattagt ataattatag cacgaaaagg atctaggtga agatcctttt
      481 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
      541 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      601 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      661 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      721 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      781 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      841 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      901 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      961 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1021 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1081 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1141 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1201 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1261 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1321 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1381 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1441 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1501 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     1561 ttacgccaag cttggctgca ggtcgacgga tccccgggaa ttcactggcc gtcgttttac
     1621 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     1681 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     1741 gcagcctgaa tggcgaatgg cgactaacgg ggcaggttag tgacattaga aaaccgactg
     1801 taaaaagtac agtcggcatt atctcatatt ataaaagcca gtcattaggc ctatctgaca
     1861 attcctgaat agagttcata aacaatcctg catgataacc atcacaaaca gaatgatgta
     1921 cctgtaaaga tagcggtaaa tatattgaat tacctttatt aatgaatttt cctgctgtaa
     1981 taatgggtag aaggtaatta ctattattat tgatatttaa gttaaaccca gtaaatgaag
     2041 tccatggaat aatagaaaga gaaaaagcat tttcaggtat aggtgttttg ggaaacaatt
     2101 tccccgaacc attatatttc tctacatcag aaaggtataa atcataaaac tctttgaagt
     2161 cattctttac aggagtccaa ataccagaga atgttttaga tacaccatca aaaattgtat
     2221 aaagtggctc taacttatcc caataaccta actctccgtc gctattgtaa ccagttctaa
     2281 aagctgtatt tgagtttatc acccttgtca ctaagaaaat aaatgcaggg taaaatttat
     2341 atccttcttg ttttatgttt cggtataaaa cactaatatc aatttctgtg gttatactaa
     2401 aagtcgtttg ttggttcaaa taatgattaa atatctcttt tctcttccaa ttgtctaaat
     2461 caattttatt aaagttcatt tgatatgcct cctaaatttt tatctaaagt gaatttagga
     2521 ggcttacttg tctgctttct tcattagaat caatcctttt ttaaaagtca atattactgt
     2581 aacataaata tatattttaa aaatatccca ctttatccaa ttttcgtttg ttgaactaat
     2641 gggtgcttta gttgaagaat aaaagaccac attaaaaaat gtggtctttt gtgttttttt
     2701 aaaggatttg agcgtagcga aaaatccttt tctttcttat cttgatacta tatagaaaca
     2761 acatcatttt tcaaaattag gtcaaagcct tgtgtatcaa gggtttgatg gttctttgac
     2821 aggtaaaaac tccttctgct attattaagg tgtcgaatca aaataataga atgctagaga
     2881 actagctcag aaggagtttt tttgttgatt tattcatctg aaaatgatta tagcatcctc
     2941 gaagataaaa ccgcaacagg taaaaagcgg gattggaagg ggaaaaagag acggacgaac
     3001 ctcatggcgg agcattacga agcgttagag agtaagattg gggcacctta ctatggcaaa
     3061 aaggctgaaa aactaattag ttgtgcagag tatctttcgt ttaagagaga cccggagacg
     3121 ggcaagttaa aactgtatca agcccatttt tgtaaagtga ggttatgtcc gatgtgtgcg
     3181 tggcgcaggt cgttaaaaat tgcttatcac aataagttga tcgtagagga agccaataga
     3241 cagtacggct gcggatggat ttttctcacg ctgacgattc gaaatgtaaa gggagaacgg
     3301 ctgaagccac aaatttctgc gatgatggaa ggctttagga aactgttcca gtacaaaaaa
     3361 gtaaaaactt cggttcttgg atttttcaga gctttagaga ttaccaaaaa tcatgaagaa
     3421 gatacatatc atcctcattt tcatgtgttg ataccagtaa ggaaaaatta ttttgggaaa
     3481 aactatatta agcaggcgga gtggacgagc ctttggaaaa aggcgatgaa attggattac
     3541 actccaattg tcgatattcg tcgagtgaaa ggtaaagcta agattgacgc tgaacagatt
     3601 gaaaacgatg tgcggaacgc aatgatggag caaaaagctg ttctcgaaat ctctaaatat
     3661 ccggttaagg atacggatgt tgtgcgcggt aataaggtga ctgaagacaa tctgaacacg
     3721 gtgctttact tggatgatgc gttggcagct cgaaggttaa ttggatacgg tggcattttg
     3781 aaggagatac ataaagagct gaatcttggt gatgcggagg acggcgatct ggtcaagatt
     3841 gaggaagaag atgacgaggt tgcaaatggt gcatttgagg ttatggctta ttggcatcct
     3901 ggcattaaaa attacataat caaataaaaa aagcagacct ttagaaggcc tgctttttta
     3961 actaacccat ttgtattgtg ttgaaatatg ttttgtatgg tgcactctca gtacaatctg
     4021 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     4081 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     4141 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     4201 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagcgat tcacaaaaaa
     4261 taggcacacg aaaaacaagt taagggatgc agtttatgca tcccttaact tacttattaa
     4321 ataatttata gctattgaaa agagataaga attgttcaaa gctaatattg tttaaatcgt
     4381 caattcctgc atgttttaag gaattgttaa attgattttt tgtaaatatt ttcttgtatt
     4441 ctttgttaac ccatttcata acgaaataat tatacttttg tttatctttg tgtgatattc
     4501 ttgatttttt tctacttaat ctgataagtg agctattcac tttaggttta ggatgaaaat
     4561 attctcttgg aaccatactt aatatagaaa tatcaacttc tgccattaaa agtaatgcca
     4621 atgagcgttt tgtatttaat aatcttttag caaacccgta ttccacgatt aaataaatct
     4681 cattagctat actatcaaaa acaattttgc gtattatatc cgtacttatg ttataaggta
     4741 tattacca
//
