LOCUS       V012786                 5100 bp    DNA     circular SYN 17-NOV-2021
DEFINITION  Exported.
ACCESSION   V012786
VERSION     V012786
KEYWORDS    pHP13-P43
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Jakobsen OM, Benichou A, Flickinger MC, Valla S, Ellingsen TE,
            Brautaset T.
  TITLE     Upregulated transcription of plasmid and chromosomal ribulose
            monophosphate pathway genes is critical for methanol assimilation
            rate and methanol tolerance in the methylotrophic bacterium Bacillus
            methanolicus
  JOURNAL   J. Bacteriol. 188 (8), 3063-3072 (2006)
   PUBMED   16585766
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Jakobsen OM, Benichou A, Flickinger MC, Valla S, Ellingsen TE,
            Brautaset T.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2005) Department of Biotechnology, Norwegian
            University of Science and Technology, Sem Saelands vei 6/8,
            Trondheim 7491, Norway
REFERENCE   3  (bases 1 to 5100)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5100)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J.
            Bacteriol."; date: "2006"; volume: "188"; issue: "8"; pages:
            "3063-3072"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (18-NOV-2005) Department of Biotechnology, Norwegian University of
            Science and Technology, Sem Saelands vei 6/8, Trondheim 7491,
            Norway"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5100
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      565..1153
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    1441..1462
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1477..1507
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    1515..1531
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1539..1555
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(1956..1972)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(2184..2831)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(join(4665..5100,1..296))
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
ORIGIN
        1 tatattttat aggattggtt tttaggaaat ttaaactgca atatatcctt gtttaaaact
       61 tggaaattat cgtgatcaac aagtttattt tctgtagttt tgcataattt atggtctatt
      121 tcaatggcag ttacgaaatt acacctcttt actaattcaa gggtaaaatg gccttttcct
      181 gagccgattt caaagatatt atcatgttca tttaatctta tatttgtcat tattttatct
      241 atattatgtt ttgaagtaat aaagttttga ctgtgtttta tatttttctc gttcattata
      301 accctcttta atttggttat atgaattttg cttattaacg attcattata accacttatt
      361 ttttgtttgg ttgataatga actgtgctga ttacaaaaat actaaaaatg cccatatttt
      421 ttcctcctta taaaattagt ataattatag cacgaaaagg atctaggtga agatcctttt
      481 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
      541 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
      601 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
      661 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
      721 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
      781 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
      841 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
      901 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
      961 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1021 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1081 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1141 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1201 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1261 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1321 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1381 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1441 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1501 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     1561 ttacgccaag cttgcggctt ccttgtagag ctcagcatta ttgagtggat gattatattc
     1621 cttttgatag gtggtatgtt ttcgcttgaa cttttaaata cagccattga acatacggtt
     1681 gatttaataa ctgacaaaca tcaccctctt gctaaagcgg ccaaggacgc tgccgccggg
     1741 gctgtttgcg tttttgccgt gatttcgtgt atcattggtt tacttatttt tttgccaaag
     1801 ctgtaatggc tgaaaattct tacatttatt ttacattttt agaaatgggc gtgaaaaaaa
     1861 gcgcgcgatt atgtaaaata taaagtgata gcggtaccat tataggtaag agaggaatgt
     1921 acacatgctg caggtcgacg gatccccggg aattcactgg ccgtcgtttt acaacgtcgt
     1981 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     2041 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     2101 aatggcgaat ggcgactaac ggggcaggtt agtgacatta gaaaaccgac tgtaaaaagt
     2161 acagtcggca ttatctcata ttataaaagc cagtcattag gcctatctga caattcctga
     2221 atagagttca taaacaatcc tgcatgataa ccatcacaaa cagaatgatg tacctgtaaa
     2281 gatagcggta aatatattga attaccttta ttaatgaatt ttcctgctgt aataatgggt
     2341 agaaggtaat tactattatt attgatattt aagttaaacc cagtaaatga agtccatgga
     2401 ataatagaaa gagaaaaagc attttcaggt ataggtgttt tgggaaacaa tttccccgaa
     2461 ccattatatt tctctacatc agaaaggtat aaatcataaa actctttgaa gtcattcttt
     2521 acaggagtcc aaataccaga gaatgtttta gatacaccat caaaaattgt ataaagtggc
     2581 tctaacttat cccaataacc taactctccg tcgctattgt aaccagttct aaaagctgta
     2641 tttgagttta tcacccttgt cactaagaaa ataaatgcag ggtaaaattt atatccttct
     2701 tgttttatgt ttcggtataa aacactaata tcaatttctg tggttatact aaaagtcgtt
     2761 tgttggttca aataatgatt aaatatctct tttctcttcc aattgtctaa atcaatttta
     2821 ttaaagttca tttgatatgc ctcctaaatt tttatctaaa gtgaatttag gaggcttact
     2881 tgtctgcttt cttcattaga atcaatcctt ttttaaaagt caatattact gtaacataaa
     2941 tatatatttt aaaaatatcc cactttatcc aattttcgtt tgttgaacta atgggtgctt
     3001 tagttgaaga ataaaagacc acattaaaaa atgtggtctt ttgtgttttt ttaaaggatt
     3061 tgagcgtagc gaaaaatcct tttctttctt atcttgatac tatatagaaa caacatcatt
     3121 tttcaaaatt aggtcaaagc cttgtgtatc aagggtttga tggttctttg acaggtaaaa
     3181 actccttctg ctattattaa ggtgtcgaat caaaataata gaatgctaga gaactagctc
     3241 agaaggagtt tttttgttga tttattcatc tgaaaatgat tatagcatcc tcgaagataa
     3301 aaccgcaaca ggtaaaaagc gggattggaa ggggaaaaag agacggacga acctcatggc
     3361 ggagcattac gaagcgttag agagtaagat tggggcacct tactatggca aaaaggctga
     3421 aaaactaatt agttgtgcag agtatctttc gtttaagaga gacccggaga cgggcaagtt
     3481 aaaactgtat caagcccatt tttgtaaagt gaggttatgt ccgatgtgtg cgtggcgcag
     3541 gtcgttaaaa attgcttatc acaataagtt gatcgtagag gaagccaata gacagtacgg
     3601 ctgcggatgg atttttctca cgctgacgat tcgaaatgta aagggagaac ggctgaagcc
     3661 acaaatttct gcgatgatgg aaggctttag gaaactgttc cagtacaaaa aagtaaaaac
     3721 ttcggttctt ggatttttca gagctttaga gattaccaaa aatcatgaag aagatacata
     3781 tcatcctcat tttcatgtgt tgataccagt aaggaaaaat tattttggga aaaactatat
     3841 taagcaggcg gagtggacga gcctttggaa aaaggcgatg aaattggatt acactccaat
     3901 tgtcgatatt cgtcgagtga aaggtaaagc taagattgac gctgaacaga ttgaaaacga
     3961 tgtgcggaac gcaatgatgg agcaaaaagc tgttctcgaa atctctaaat atccggttaa
     4021 ggatacggat gttgtgcgcg gtaataaggt gactgaagac aatctgaaca cggtgcttta
     4081 cttggatgat gcgttggcag ctcgaaggtt aattggatac ggtggcattt tgaaggagat
     4141 acataaagag ctgaatcttg gtgatgcgga ggacggcgat ctggtcaaga ttgaggaaga
     4201 agatgacgag gttgcaaatg gtgcatttga ggttatggct tattggcatc ctggcattaa
     4261 aaattacata atcaaataaa aaaagcagac ctttagaagg cctgcttttt taactaaccc
     4321 atttgtattg tgttgaaata tgttttgtat ggtgcactct cagtacaatc tgctctgatg
     4381 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     4441 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     4501 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4561 ttttataggt taatgtcatg ataataatgg tttcttagcg attcacaaaa aataggcaca
     4621 cgaaaaacaa gttaagggat gcagtttatg catcccttaa cttacttatt aaataattta
     4681 tagctattga aaagagataa gaattgttca aagctaatat tgtttaaatc gtcaattcct
     4741 gcatgtttta aggaattgtt aaattgattt tttgtaaata ttttcttgta ttctttgtta
     4801 acccatttca taacgaaata attatacttt tgtttatctt tgtgtgatat tcttgatttt
     4861 tttctactta atctgataag tgagctattc actttaggtt taggatgaaa atattctctt
     4921 ggaaccatac ttaatataga aatatcaact tctgccatta aaagtaatgc caatgagcgt
     4981 tttgtattta ataatctttt agcaaacccg tattccacga ttaaataaat ctcattagct
     5041 atactatcaa aaacaatttt gcgtattata tccgtactta tgttataagg tatattacca
//
