LOCUS       V005530                11568 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005530
VERSION     V005530
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 11568)
  AUTHORS   Alexander WG, Peris D, Pfannenstiel BT, Opulente DA, Kuang M,
            Hittinger CT.
  TITLE     Efficient engineering of marker-free synthetic allotetraploids of
            Saccharomyces
  JOURNAL   Fungal Genet. Biol. (2015) In press
   PUBMED   26555931
REFERENCE   2  (bases 1 to 11568)
  AUTHORS   Alexander WG, Peris D, Pfannenstiel B, Opulente DA, Kuang M,
            Hittinger CT.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-SEP-2015) Laboratory of Genetics, University of
            Wisconsin-Madison, 425-G Henry Mall, 4350 Genetics Biotechnology
            Center, Madison, WI 53706, USA
REFERENCE   3  (bases 1 to 11568)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11568)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal
            Genet. Biol. (2015) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-SEP-2015) Laboratory of Genetics, University of
            Wisconsin-Madison, 425-G Henry Mall, 4350 Genetics Biotechnology
            Center, Madison, WI 53706, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11568
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(31..1035)
                     /label="tTA"
                     /note="tetracycline-controlled transactivator, comprising a
                     fusion of the tetracycline repressor TetR with the
                     C-terminal activation domain of herpes simplex virus VP16"
     promoter        complement(1140..1343)
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     regulatory      complement(1334..1723)
                     /regulatory_class="enhancer"
     enhancer        1344..1723
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     terminator      1835..2022
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     protein_bind    2052..2322
                     /label="tetracycline response element"
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     CDS             2510..4267
                     /gene="HO"
                     /label="Homothallic switching endonuclease"
                     /note="Homothallic switching endonuclease from
                     Saccharomyces cerevisiae (strain ATCC 204508 / S288c).
                     Accession#: P09932"
     CDS             4316..4813
                     /label="13xMyc"
                     /note="13 tandem Myc epitope tags"
     terminator      5006..5253
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     primer_bind     complement(5276..5292)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    5300..5316
                     /label="lac operator"
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5324..5354)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5369..5390)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5678..6266)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6440..7297)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(7298..7402)
                     /label="AmpR promoter"
     gene            7610..9185
                     /label="hphMX6"
                     /note="yeast selectable marker conferring hygromycin
                     resistance"
     rep_origin      9528..9981
                     /label="ARS1"
                     /note="S. cerevisiae autonomously replicating sequence
                     ARS1"
     misc_feature    10501..10793
                     /label="CEN4"
                     /note="S. cerevisiae CEN4 centromere"
     primer_bind     11552..11568
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 gaattcttat tacgatcctc gcgcccccta cccaccgtac tcgtcaattc caagggcatc
       61 ggtaaacatc tgctcaaact cgaagtcggc catatccaga gcgccgtagg gggcggagtc
      121 gtggggggta aatcccggac ccggggaatc cccgtccccc aacatgtcca gatcgaaatc
      181 gtctagcgcg tcggcatgcg ccatcgccac gtcctcgccg tctaagtgga gctcgtcccc
      241 caggctgaca tcggtcgggg gggccgtcga cagtctgcgc gtgtgtcccg cggggagaaa
      301 ggacaggcgc ggagccgcca gccccgcctc ttcgggggcg tcgtcgtccg ggagatcgag
      361 caggccctcg atggtagacc cgtaattgtt tttcgtacgc gcgcggctgt acgcggaccc
      421 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa
      481 ggctggctct gcaccttggc gatcaaataa ttcgatagct tgtcgtaata atggcggcat
      541 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gttcttccaa
      601 tacgcaacct aaagtaaagt gccccacagc gctgagtgca tataatgcat tctctagtga
      661 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
      721 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
      781 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
      841 agtatggtgc ctatctaaca tctcaatggg taaggcgtcg agcaaagccc gcttattttt
      901 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
      961 taaaccttcg attccgaccc cattaagcag ctctaatgcg ccgttaatca ctttactttt
     1021 atctaatcta gacatatgaa ttaattcggg ccgcggaggc tggatcggtc ccggtgtctt
     1081 ctatggaggt caaaacagcg tggatggcgt ctccaggcga tctgacggtt cactaaacga
     1141 gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg
     1201 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg
     1261 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg
     1321 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc
     1381 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg
     1441 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg
     1501 gcagtttagc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact
     1561 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc
     1621 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac
     1681 cccgtaattg attactatta ataactagtc aataatcaat gtcaacatgg cggtaatgtt
     1741 ggacatgagc caatataaat gtacatatta tgatatggat acaacgtatg caatgggcca
     1801 agctcctcga gtaattcgcg ccacttctaa ataagcgaat ttcttatgat ttatgatttt
     1861 tattattaaa taagttataa aaaaaataag tgtatacaaa ttttaaagtg actcttaggt
     1921 tttaaaacga aaattcttgt tcttgagtaa ctctttcctg taggtcaggt tgctttctca
     1981 ggtatagcat gaggtcgctc ttattgacca cacctctacc ggcagatcaa ttcctcgatc
     2041 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2101 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2161 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2221 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2281 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagctc
     2341 ggtaccctat ggcatgcatg tgctctgtat gtatataaaa ctcttgtttt cttcttttct
     2401 ctaaatattc tttccttata cattaggtcc tttgtagcat aaattactat acttctatag
     2461 acacgcaaac acaaatacac acactaaatt accggatcaa ttcgggggaa tgctttctga
     2521 aaacacgact attctgatgg ctaacggtga aattaaagac atcgcaaacg tcacggctaa
     2581 ctcttacgtt atgtgcgcag atggctccgc tgcccgcgtc ataaatgtca cacagggcta
     2641 tcagaaaatc tataatatac agcaaaaaac caaacacaga gcttttgaag gtgaacctgg
     2701 taggttagat cccaggcgta gaacagttta tcagcgtctt gcattacaat gtactgcagg
     2761 tcataaattg tcagtcaggg tccctaccaa accactgttg gaaaaaagtg gtagaaatgc
     2821 caccaaatat aaagtgagat ggagaaatct gcagcaatgt cagacgcttg atggtaggat
     2881 aataataatt ccaaaaaacc atcataagac attcccaatg acagttgaag gtgagtttgc
     2941 cgcaaaacgc ttcatagaag aaatggagcg ctctaaagga gaatatttca actttgacat
     3001 tgaagttaga gatttggatt atcttgatgc tcaattgaga atttctagct gcataagatt
     3061 tggtccagta ctcgcaggaa atggtgtttt atctaaattt ctcactggac gtagtgacct
     3121 tgtaactcct gctgtaaaaa gtatggcttg gatgcttggt ctgtggttag gtgacagtac
     3181 aacaaaagag ccagaaatct cagtagatag cttggatcct aagctaatgg agagtttaag
     3241 agaaaatgcg aaaatctggg gtctctacct tacggtttgt gacgatcacg ttccgctacg
     3301 tgccaaacat gtaaggcttc attatggaga tggtccagat gaaaacagga agacaaggaa
     3361 tttgaggaaa aataatccat tctggaaagc tgtcacaatt ttaaagttta aaagggatct
     3421 tgatggagag aagcaaatcc ctgaatttat gtacggcgag catatagaag ttcgtgaagc
     3481 attcttagcc ggcttgatcg actcagatgg gtacgttgtg aaaaagggcg aaggccctga
     3541 atcttataaa atagcaattc aaactgttta ttcatccatt atggacggaa ttgtccatat
     3601 ttcaagatct cttggtatgt cagctactgt gacgaccagg tcagctaggg aggaaatcat
     3661 tgaaggaaga aaagtccaat gtcaatttac atacgactgt aatgttgctg ggggaacaac
     3721 ttcacagaat gttttgtcat attgtcgaag tggtcacaaa acaagagaag ttccgccaat
     3781 tataaaaagg gaacccgtat atttcagctt cacggatgat ttccagggtg agagtactgt
     3841 atatgggctt acgatagaag gccataaaaa tttcttgctt ggcaacaaaa tagaagtgaa
     3901 atcatgtcga ggctgctgtg tgggagaaca gcttaaaata tcacaaaaaa agaatctaaa
     3961 acactgtgtt gcttgtccca gaaagggaat caagtatttt tataaagatt ggagtggtaa
     4021 aaatcgagta tgtgctagat gctatggaag atacaaattc agcggtcatc actgtataaa
     4081 ttgcaagtat gtaccagaag cacgtgaagt gaaaaaggca aaagacaaag gcgaaaaatt
     4141 gggcattacg cccgaaggtt tgccagttaa aggaccagag tgtataaaat gtggcggaat
     4201 cttacagttt gatgctgtcc gcgggcctca taagagttgt ggtaacaacg caggtgcgcg
     4261 catctgccgg atccccgggt taattaacgg tggaggtgga ggtggaggtg gaggtgaaca
     4321 aaagctaatc tccgaggaag acttgaacgg tgaacaaaaa ttaatctcag aagaagactt
     4381 gaacggactc gacggtgaac aaaagttgat ttctgaagaa gatttgaacg gtgaacaaaa
     4441 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa
     4501 cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct
     4561 aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg
     4621 actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat
     4681 ctccgaggaa gacttgaacg gtgaacaaaa attaatctca gaagaagact tgaacggact
     4741 cgacggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc
     4801 cgaggaagac ttgaacggtg aacaaaaatt aatctcagaa gaagacttga acggactcga
     4861 cggtgaacaa aagttgattt ctgaagaaga tttgaacggt gaacaaaagc taatctccga
     4921 ggaagacttg aacggtgaac aaaaattaat caatcactag tgaattcgcg ccacttctaa
     4981 ataagcgaat ttcttgaggg ccgcatcatg taattagtta tgtcacgctt acattcacgc
     5041 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc
     5101 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt
     5161 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag
     5221 aaggttttgg gacgctcgaa ggctttaatt tgcggccaag cttggcgtaa tcatggtcat
     5281 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     5341 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     5401 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     5461 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     5521 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     5581 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     5641 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     5701 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     5761 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     5821 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     5881 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     5941 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6001 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6061 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     6121 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     6181 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     6241 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     6301 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     6361 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     6421 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     6481 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     6541 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     6601 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     6661 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     6721 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     6781 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     6841 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     6901 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     6961 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7021 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7081 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7141 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     7201 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     7261 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     7321 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     7381 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     7441 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
     7501 aagaattcag ctgaagcttc gtacgctgca ggtcgacgga tccccgggtt aattaaggcg
     7561 cgccagatct gtttagcttg ccttgtcccc gccgggtcac ccggccagcg acatggaggc
     7621 ccagaatacc ctccttgaca gtcttgacgt gcgcagctca ggggcatgat gtgactgtcg
     7681 cccgtacatt tagcccatac atccccatgt ataatcattt gcatccatac attttgatgg
     7741 ccgcacggcg cgaagcaaaa attacggctc ctcgctgcag acctgcgagc agggaaacgc
     7801 tcccctcaca gacgcgttga attgtcccca cgccgcgccc ctgtagagaa atataaaagg
     7861 ttaggatttg ccactgaggt tcttctttca tatacttcct tttaaaatct tgctaggata
     7921 cagttctcac atcacatccg aacataaaca accatgggta aaaagcctga actcaccgcg
     7981 acgtctgtcg agaagtttct gatcgaaaag ttcgacagcg tctccgacct gatgcagctc
     8041 tcggagggcg aagaatctcg tgctttcagc ttcgatgtag gagggcgtgg atatgtcctg
     8101 cgggtaaata gctgcgccga tggtttctac aaagatcgtt atgtttatcg gcactttgca
     8161 tcggccgcgc tcccgattcc ggaagtgctt gacattgggg aattcagcga gagcctgacc
     8221 tattgcatct cccgccgtgc acagggtgtc acgttgcaag acctgcctga aaccgaactg
     8281 cccgctgttc tgcagccggt cgcggaggcc atggatgcga tcgctgcggc cgatcttagc
     8341 cagacgagcg ggttcggccc attcggaccg caaggaatcg gtcaatacac tacatggcgt
     8401 gatttcatat gcgcgattgc tgatccccat gtgtatcact ggcaaactgt gatggacgac
     8461 accgtcagtg cgtccgtcgc gcaggctctc gatgagctga tgctttgggc cgaggactgc
     8521 cccgaagtcc ggcacctcgt gcacgcggat ttcggctcca acaatgtcct gacggacaat
     8581 ggccgcataa cagcggtcat tgactggagc gaggcgatgt tcggggattc ccaatacgag
     8641 gtcgccaaca tcttcttctg gaggccgtgg ttggcttgta tggagcagca gacgcgctac
     8701 ttcgagcgga ggcatccgga gcttgcagga tcgccgcggc tccgggcgta tatgctccgc
     8761 attggtcttg accaactcta tcagagcttg gttgacggca atttcgatga tgcagcttgg
     8821 gcgcagggtc gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa
     8881 atcgcccgca gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt
     8941 ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat aatcagtact gacaataaaa
     9001 agattcttgt tttcaagaac ttgtcatttg tatagttttt ttatattgta gttgttctat
     9061 tttaatcaaa tgttagcgtg atttatattt tttttcgcct cgacatcatc tgcccagatg
     9121 cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg tatgtgaatg ctggtcgcta
     9181 tactgctgtc gattcgatac taacgccgcc atccagtgtc gaaaacgagc tcgaattcat
     9241 cgatgatatc agatccacta gtggcctatg caattctcat gtttgacagc ttatcatcga
     9301 tcgtccaact gcatggagat gagtcgtggc aagaatacca agagttcctc ggtttgccag
     9361 ttattaaaag actcgtattt ccaaaagact gcaacatact actcagtgca gcttcacaga
     9421 aacctcattc gtttattccc ttgtttgatt cagaagcagg tgggacaggt gaacttttgg
     9481 attggaactc gatttctgac tgggttggaa ggcaagagag ccccgagagc ttacatttta
     9541 tgttagctgg tggactgacg ccagaaaatg ttggtgatgc gcttagatta aatggcgtta
     9601 ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt aaaagactct aacaaaatag
     9661 caaatttcgt caaaaatgct aagaaatagg ttattactga gtagtattta tttaagtatt
     9721 gtttgtgcac ttgcctgcaa gccttttgaa aagcaagcat aaaagatcta aacataaaat
     9781 ctgtaaaata acaagatgta aagataatgc taaatcattt ggctttttga ttgattgtac
     9841 aggaaaatat acatcgcagg gggttgactt ttaccatttc accgcaatgg aatcaaactt
     9901 gttgaagaga atgttcacag gcgcatacgc tacaatgacc cgattcttgc tagccttttc
     9961 tcggtcttgc aaacaaccgc cggcagctta gtatataaat acacatgtac atacctctct
    10021 ccgtatcctc gtaatcattt tcttgtattt atcgtctttt cgctgtaaaa actttatcac
    10081 acttatctca aatacactta ttaaccgctt ttactattat cttctacgct gacagtaata
    10141 tcaaacagtg acacatatta aacacagtgg tttctttgca taaacaccat cagcctcaag
    10201 tcgtcaagta aagatttcgt gttcatgcag atagataaca atctatatgt tgataattag
    10261 cgttgcctca tcaatgcgag atccgtttaa ccggacccta gtgcacttac cccacgttcg
    10321 gtccactgtg tgccgaacat gctccttcac tattttaaca tgtggaatta tcgatgccgg
    10381 tgttattctt gctatgatag gccatcgaat gttaaaggga agatttattg ttttgccctt
    10441 taaagacgaa tttggaccgt gaatgtttaa ataattctca tgtttgacag cttatcatcg
    10501 aactctaaga ggtgatactt atttactgta aaactgtgac gataaaaccg gaaggaagaa
    10561 taagaaaact cgaactgatc tataatgcct attttctgta aagagtttaa gctatgaaag
    10621 cctcggcatt ttggccgctc ctaggtagtg ctttttttcc aaggacaaaa cagtttcttt
    10681 ttcttgagca ggttttatgt ttcggtaatc ataaacaata aataaattat ttcatttatg
    10741 tttaaaaata aaaaataaaa aagtatttta aatttttaaa aaagttgatt ataagcatgt
    10801 gaccttttgc aagcaattaa attttgcaat ttgtgatttt aggcaaaagt tacaatttct
    10861 ggctcgtgta atatatgtat gctaaagtga acttttacaa agtcgatatg gacttagtca
    10921 aaagaaattt tcttaaaaat atatagcact agccaattta gcacttcttt atgagatata
    10981 ttatagactt tattaagcca gatttgtgta ttatatgtat ttacccggcg aatcatggac
    11041 atacattctg aaataggtaa tattctctat ggtgagacag catagataac ctaggataca
    11101 agttaaaagc tagtactgtt ttgcagtaat ttttttcttt tttataagaa tgttaccacc
    11161 taaataagtt ataaagtcaa tagttaagtt tgatatttga ttgtaaaata ccgtaatata
    11221 tttgcatgat caaaaggctc aatgttgact agccagcatg tcaaccacta tattgatcac
    11281 cgatatatgg acttccacac caactagtaa tatgacaata aattcaagat attcttcatg
    11341 agaatggccc agcgatatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
    11401 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
    11461 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
    11521 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagt
//
