LOCUS       V005535                 3523 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005535
VERSION     V005535
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Zhang J, Liu S, Li R, Hong W, Xiao Y, Feng Y, Cui Q, Liu YJ.
  TITLE     Efficient whole-cell-catalyzing cellulose saccharification using
            engineered Clostridium thermocellum
  JOURNAL   Biotechnol Biofuels 10, 124 (2017)
   PUBMED   28507596
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Zhang J, Liu S, Li R, Hong W, Xiao Y, Feng Y, Cui Q, Liu Y-J.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-MAR-2017) Metabolomics Group, Qingdao Institute of
            Bioenergy and Bioprocess Technology, Chinese Academy of Sciences,
            189 Songling Rd., Qingdao 266101, P. R. China
REFERENCE   3  (bases 1 to 3523)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3523)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol
            Biofuels 10, 124 (2017)"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (21-MAR-2017) Metabolomics Group, Qingdao Institute of Bioenergy and
            Bioprocess Technology, Chinese Academy of Sciences, 189 Songling
            Rd., Qingdao 266101, P. R. China"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3523
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(110..131)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             complement(264..911)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(1021..2022)
                     /label="repB"
                     /note="RepB replication protein"
     rep_origin      complement(2714..3302)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ctgcagaacg gcgcccgggg atccctcgag gaattctcta gaaagcttgg tacccatatg
       61 gctagcacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      121 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      181 agcccttcaa acttcccaaa ggcgagccct agtgacatta gaaaaccgac tgtaaaaagt
      241 acagtcggca ttatctcata ttataaaagc cagtcattag gcctatctga caattcctga
      301 atagagttca taaacaatcc tgcatgataa ccatcacaaa cagaatgatg tacctgtaaa
      361 gatagcggta aatatattga attaccttta ttaatgaatt ttcctgctgt aataatgggt
      421 agaaggtaat tactattatt attgatattt aagttaaacc cagtaaatga agtccatgga
      481 ataatagaaa gagaaaaagc attttcaggt ataggtgttt tgggaaacaa tttccccgaa
      541 ccattatatt tctctacatc agaaaggtat aaatcataaa actctttgaa gtcattcttt
      601 acaggagtcc aaataccaga gaatgtttta gatacaccat caaaaattgt ataaagtggc
      661 tctaacttat cccaataacc taactctccg tcgctattgt aaccagttct aaaagctgta
      721 tttgagttta tcacccttgt cactaagaaa ataaatgcag ggtaaaattt atatccttct
      781 tgttttatgt ttcggtataa aacactaata tcaatttctg tggttatact aaaagtcgtt
      841 tgttggttca aataatgatt aaatatctct tttctcttcc aattgtctaa atcaatttta
      901 ttaaagttca tttgatatgc ctcctaaatt tttatctaaa gtgaatttag gaggcttact
      961 tgtctgcttt cttcattaga atcaatcctt ttttaaaagt caatcccgtt tgttgaacta
     1021 ctctttaata aaataatttt tccgttccca attccacatt gcaataatag aaaatccatc
     1081 ttcatcggct ttttcgtcat catctgtatg aatcaaatcg ccttcttctg tgtcatcaag
     1141 gtttaatttt ttatgtattt cttttaacaa accaccatag gagattaacc ttttacggtg
     1201 taaaccttcc tccaaatcag acaaacgttt caaattcttt tcttcatcat cggtcataaa
     1261 atccgtatcc tttacaggat attttgcagt ttcgtcaatt gccgattgta tatccgattt
     1321 atatttattt ttcggtcgaa tcatttgaac ttttacattt ggatcatagt ctaatttcat
     1381 tgcctttttc caaaattgaa tccattgttt ttgattcacg tagttttctg tattcttaaa
     1441 ataagttggt tccacacata ccaatacatg catgtgctga ttataagaat tatctttatt
     1501 atttattgtc acttccgttg cacgcataaa accaacaaga tttttattaa tttttttata
     1561 ttgcatcatt cggcgaaatc cttgagccat atctgacaaa ctcttattta attcttcgcc
     1621 atcataaaca tttttaactg ttaatgtgag aaacaaccaa cgaactgttg gcttttgttt
     1681 aataacttca gcaacaacct tttgtgactg aatgccatgt ttcattgctc tcctccagtt
     1741 gcacattgga caaagcctgg atttacaaaa ccacactcga tacaactttc tttcgcctgt
     1801 ttcacgattt tgtttatact ctaatatttc agcacaatct tttactcttt cagccttttt
     1861 aaattcaaga atatgcagaa gttcaaagta atcaacatta gcgattttct tttctctcca
     1921 tggtctcact tttccacttt ttgtcttgtc cactaaaacc cttgattttt catctgaata
     1981 aatgctacta ttaggacaca taatattaaa agaaaccccc atctatttag ttatttgttt
     2041 ggtcacttat aactttaaca gatggggttt ttctgtgcaa ccaattttaa gggttttcaa
     2101 tactttaaaa cacatacata ccaacacttc aacgcacctt tcagcaacta aaataaaaat
     2161 gacgttattt ctatatgtat caagaataga aagaactcgt ttttcgctac gctcaaaacg
     2221 caaaaaaagc actcattcga gtgctttttc ttatcgctcc aaatcatgcg attttttcct
     2281 ctttgctttt ctttgctcac gaagttctcg atcacgctgc aaaacatctt gaagcgaaaa
     2341 agtattcttc ttttcttccg atcgctcatg ctgacgcacg aaaagccctc taggcgcata
     2401 ggaacaactc ctaaatgcat gtgaggggtt ttctcgtcca tgtgaacagt cgcatacgca
     2461 atattttgtt tcccatactg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     2521 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2581 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2641 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     2701 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     2761 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     2821 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     2881 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     2941 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3001 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3061 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3121 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3181 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3241 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3301 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3361 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3421 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3481 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcg
//
