LOCUS       V005536                 9053 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005536
VERSION     V005536
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9053)
  AUTHORS   Plagge A.
  TITLE     Multimodal cell tracking from implantation to tumour growth by
            combining gold nanorods, iRFP720 and luciferase labelling
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9053)
  AUTHORS   Plagge A.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2017) Cellular and Molecular Physiology,
            University of Liverpool, Crown Street, Liverpool L69 3BX, UK
REFERENCE   3  (bases 1 to 9053)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9053)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-AUG-2017) Cellular and Molecular Physiology, University of
            Liverpool, Crown Street, Liverpool L69 3BX, UK"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9053
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     repeat_region   835..1015
                     /rpt_family="HIV-5'LTR"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1686..1919
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2104..2148
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2297..2338
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2446..2563
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2627..3805
                     /label="EF-1-alpha promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3870..4817
                     /label="iRFP720"
                     /note="near-infrared fluorescent protein with an emission
                     peak at 720 nm, engineered from a bacterial phytochrome
                     (Shcherbakova and Verkhusha, 2013)"
     CDS             4818..4877
                     /label="E2A"
                     /note="2A peptide from equine rhinitis A virus polyprotein"
     CDS             4878..6527
                     /label="luciferase"
                     /note="firefly luciferase"
     primer_bind     complement(6533..6549)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     regulatory      6722..6743
                     /label="U3PPT"
                     /note="U3PPT"
                     /regulatory_class="other"
     LTR             7059..7239
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     rep_origin      complement(7301..7889)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8063..8920)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(8921..9025)
                     /label="AmpR promoter"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg gccgcgctga tcttcagacc tggaggagga
     1561 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     1621 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     1681 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     1741 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     1801 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1861 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1921 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1981 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2041 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2101 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2161 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2221 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2281 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     2341 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatcggca
     2401 ctgcgtgcgc caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg
     2461 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2521 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2581 cagcagagat ccagtttggt tagtaccggg cccgctctag ccgtgaggct ccggtgcccg
     2641 tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag gggtcggcaa
     2701 ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg
     2761 gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag tcgccgtgaa
     2821 cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt gtggttcccg
     2881 cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt ccacctggct
     2941 gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc
     3001 ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg
     3061 ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg ataagtctct
     3121 agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag atagtcttgt
     3181 aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg gcggcgacgg
     3241 ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc ggccaccgag
     3301 aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc
     3361 gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga
     3421 aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg
     3481 agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc
     3541 ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt tctcgagctt
     3601 ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt ttccccacac
     3661 tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct ccttggaatt
     3721 tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg ttcaaagttt
     3781 ttttcttcca tttcaggtgt cgtgagcggc cgctgaactg aattctgcag tcgacggtac
     3841 cgcgggcccg ggatccaccg gtcgccacca tggcggaagg atccgtcgcc aggcagcctg
     3901 acctcttgac ctgcgacgat gagccgatcc atatccccgg tgccatccaa ccgcatggac
     3961 tgctgctcgc cctcgccgcc gacatgacga tcgttgccgg cagcgacaac cttcccgaac
     4021 tcaccggact ggcgatcggc gccctgatcg gccgctctgc ggccgatgtc ttcgactcgg
     4081 agacgcacaa ccgtctgacg atcgccttgg ccgagcccgg ggcggccgtc ggagcaccga
     4141 tcactgtcgg cttcacgatg cgaaaggacg caggcttcat cggctcctgg catcgccatg
     4201 atcagctcat cttcctcgag ctcgagcctc cccagcggga cgtcgccgag ccgcaggcgt
     4261 tcttccgccg caccaacagc gccatccgcc gcctgcaggc cgccgaaacc ttggaaagcg
     4321 cctgcgccgc cgcggcgcaa gaggtgcgga agattaccgg cttcgatcgg gtgatgatct
     4381 atcgcttcgc ctccgacttc agcgggtccg tgatcgcaga ggatcggtgc gccgaggtcg
     4441 agtcaaaact aggcctgcac tatcctgcct cattcatccc ggcgcaggcc cgtcggctct
     4501 ataccatcaa cccggtacgg atcattcccg atatcaatta tcggccggtg ccggtcaccc
     4561 cagacctcaa tccggtcacc gggcggccga ttgatcttag cttcgccatc ctgcgcagcg
     4621 tctcgcccaa ccatctggag ttcatgcgca acataggcat gcacggcacg atgtcgatct
     4681 cgattttgcg cggcgagcga ctgtggggat tgatcgtttg ccatcaccga acgccgtact
     4741 acgtcgatct cgatggccgc caagcctgcg agctagtcgc ccaggttctg gcctggcaga
     4801 tcggcgtgat ggaagagcaa tgtacaaact acgctttgtt gaaactcgct ggcgatgttg
     4861 aaagtaaccc cggtcctatg gaagatgcca aaaacattaa gaagggccca gcgccattct
     4921 acccactcga agacgggacc gccggcgagc agctgcacaa agccatgaag cgctacgccc
     4981 tggtgcccgg caccatcgcc tttaccgacg cacatatcga ggtggacatt acctacgccg
     5041 agtacttcga gatgagcgtt cggctggcag aagctatgaa gcgctatggg ctgaatacaa
     5101 accatcggat cgtggtgtgc agcgagaata gcttgcagtt cttcatgccc gtgttgggtg
     5161 ccctgttcat cggtgtggct gtggccccag ctaacgacat ctacaacgag cgcgagctgc
     5221 tgaacagcat gggcatcagc cagcccaccg tcgtattcgt gagcaagaaa gggctgcaaa
     5281 agatcctcaa cgtgcaaaag aagctaccga tcatacaaaa gatcatcatc atggatagca
     5341 agaccgacta ccagggcttc caaagcatgt acaccttcgt gacttcccat ttgccacccg
     5401 gcttcaacga gtacgacttc gtgcccgaga gcttcgaccg ggacaaaacc atcgccctga
     5461 tcatgaacag tagtggcagt accggattgc ccaagggcgt agccctaccg caccgcaccg
     5521 cttgtgtccg attcagtcat gcccgcgacc ccatcttcgg caaccagatc atccccgaca
     5581 ccgctatcct cagcgtggtg ccatttcacc acggcttcgg catgttcacc acgctgggct
     5641 acttgatctg cggctttcgg gtcgtgctca tgtaccgctt cgaggaggag ctattcttgc
     5701 gcagcttgca agactataag attcaatctg ccctgctggt gcccacacta tttagcttct
     5761 tcgctaagag cactctcatc gacaagtacg acctaagcaa cttgcacgag atcgccagcg
     5821 gcggggcgcc gctcagcaag gaggtaggtg aggccgtggc caaacgcttc cacctaccag
     5881 gcatccgcca gggctacggc ctgacagaaa caaccagcgc cattctgatc acccccgaag
     5941 gggacgacaa gcctggcgca gtaggcaagg tggtgccctt cttcgaggct aaggtggtgg
     6001 acttggacac cggtaagaca ctgggtgtga accagcgcgg cgagctgtgc gtccgtggcc
     6061 ccatgatcat gagcggctac gttaacaacc ccgaggctac aaacgctctc atcgacaagg
     6121 acggctggct gcacagcggc gacatcgcct actgggacga ggacgagcac ttcttcatcg
     6181 tggaccggct gaagagcctg atcaaataca agggctacca ggtagcccca gccgaactgg
     6241 agagcatcct gctgcaacac cccaacatct tcgacgccgg ggtcgccggc ctgcccgacg
     6301 acgatgccgg cgagctgccc gccgcagtcg tcgtgctgga acacggtaaa accatgaccg
     6361 agaaggagat cgtggactat gtggccagcc aggttacaac cgccaagaag ctgcgcggtg
     6421 gtgttgtgtt cgtggacgag gtgcctaaag gactgaccgg caagttggac gcccgcaaga
     6481 tccgcgagat tctcattaag gccaagaagg gcggcaagat cgccgtgtaa atcgataccg
     6541 tcgacctcga tcgagaccta gaaaaacatg gagcaatcac aagtagcaat acagcagcta
     6601 ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt tttccagtca
     6661 cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6721 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa gatatccttg
     6781 atctgtggat ctaccacaca caaggctact tccctgattg gcagaactac acaccagggc
     6841 cagggatcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagcaag
     6901 agaaggtaga agaagccaat gaaggagaga acacccgctt gttacaccct gtgagcctgc
     6961 atgggatgga tgacccggag agagaagtat tagagtggag gtttgacagc cgcctagcat
     7021 ttcatcacat ggcccgagag ctgcatccgg actgtactgg gtctctctgg ttagaccaga
     7081 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7141 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7201 ccctcagacc cttttagtca gtgtggaaaa tctctagcag catgtgagca aaaggccagc
     7261 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     7321 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     7381 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     7441 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     7501 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     7561 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     7621 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     7681 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     7741 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     7801 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     7861 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     7921 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     7981 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     8041 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     8101 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     8161 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
     8221 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     8281 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     8341 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     8401 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     8461 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     8521 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     8581 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     8641 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     8701 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     8761 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     8821 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     8881 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     8941 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     9001 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gac
//
