LOCUS       40924_24663        4347 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHIS3NX, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4347)
  AUTHORS   Meijer AH, Ouwerkerk PB, Hoge JH.
  TITLE     Vectors for transcription factor cloning and target site 
            identification by means of genetic selection in yeast
  JOURNAL   Yeast 14 (15), 1407-1415 (1998)
  PUBMED    9848232
REFERENCE   2  (bases 1 to 4347)
  AUTHORS   Ouwerkerk PB.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2000) Molecular Cell Biology, Institute of 
            Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands
REFERENCE   3  (bases 1 to 4347)
  AUTHORS   Ouwerkerk PB.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-MAY-2001) Molecular Cell Biology, Institute of 
            Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands
REFERENCE   4  (bases 1 to 4347)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 4347)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; issue: "15"; pages: "1407-1415"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2000) Molecular Cell Biology, Institute of Molecular Plant 
            Sciences, Wassenaarseweg 64, Leiden 2333 AL, Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (11-MAY-2001) Molecular Cell Biology, Institute of Molecular Plant 
            Sciences, Wassenaarseweg 64, Leiden 2333 AL, Netherlands"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     On May 11, 2001 this sequence version replaced AF272030.1.
FEATURES             Location/Qualifiers
     source          1..4347
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(685..1323)
                     /regulatory_class="terminator"
     CDS             complement(1327..1983)
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     misc_feature    complement(2083..2130)
                     /note="multiple cloning site; unique sites are NotI, SpeI, 
                     BamHI, SmaI, EcoRI"
     promoter        complement(2159..2177)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2198..2214)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2222..2238)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2246..2276)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2291..2312)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2600..3188)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3362..4219)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4220..4324)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gactctagag gatcgatcct cggggacacc aaatatggcg atctcggcct
      721 tttcgtttct tggagctggg acatgtttgc catcgatcca tctaccacca gaacggccgt
      781 tagatctgct gccaccgttg tttccaccga agaaaccacc gttgccgtaa ccaccacgac
      841 ggttgttgct aaagaagctg ccaccgccac ggccaccgtt gtagccgccg ttgttgttat
      901 tgtagttgct catgttattt ctggcacttc ttggttttcc tcttaagtga ggaggaacat
      961 aaccattctc gttgttgtcg ttgatgctta aattttgcac ttgttcgctc agttcagcca
     1021 taatatgaaa tgcttttctt gttgttctta cggaatacca cttgccacct atcaccacaa
     1081 ctaacttttt cccgttcctc catctctttt atattttttt tctcgagttc aagagaaaaa
     1141 aaaagaaaaa gcaaaaagaa aaaaggaaag cgcgcctcgt tcagaatgac acgtatagaa
     1201 tgatgcatta ccttgtcatc ttcagtatca tactgttcgt atacatactt actgacattc
     1261 ataggtatac atatatacac atgtatatat atcgtatgct gcagctttaa ataatcggtg
     1321 tcactacata agaacacctt tggtggaggg aacatcgttg gtaccattgg gcgaggtggc
     1381 ttctcttatg gcaaccgcaa gagccttgaa cgcactctca ctacggtgat gatcattctt
     1441 gcctcgcaga caatcaacgt ggagggtaat tctgctagcc tctgcaaagc tttcaagaaa
     1501 atgcgggatc atctcgcaag agagatctcc tactttctcc ctttgcaaac caagttcgac
     1561 aactgcgtac ggcctgttcg aaagatctac caccgctctg gaaagtgcct catccaaagg
     1621 cgcaaatcct gatccaaacc tttttactcc acgcgccagt agggcctctt taaaagcttg
     1681 accgagagca atcccgcagt cttcagtggt gtgatggtcg tctatgtgta agtcaccaat
     1741 gcactcaacg attagcgacc agccggaatg cttggccaga gcatgtatca tatggtccag
     1801 aaaccctata cctgtgtgga cgttaatcac ttgcgattgt gtggcctgtt ctgctactgc
     1861 ttctgcctct ttttctggga agatcgagtg ctctatcgct aggggaccac cctttaaaga
     1921 gatcgcaatc tgaatcttgg tttcatttgt aatacgcttt actagggctt tctgctctgt
     1981 catctttgcc ttcgtttatc ttgcctgctc attttttagt atattcttcg aagaaatcac
     2041 attactttat ataatgtata attcattatg tgataatgcc aggaattcct gcagcccggg
     2101 ggatccacta gttctagcta gagcggccgc caccgcggtg gagctccagc ttttgttccc
     2161 tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     2221 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     2281 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     2341 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     2401 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     2461 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     2521 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     2581 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     2641 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     2701 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     2761 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     2821 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     2881 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     2941 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     3001 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     3061 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     3121 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     3181 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     3241 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     3301 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     3361 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     3421 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     3481 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     3541 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     3601 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     3661 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     3721 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     3781 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     3841 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     3901 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     3961 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     4021 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     4081 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     4141 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     4201 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     4261 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     4321 cgcgcacatt tccccgaaaa gtgccac
//
