LOCUS       40924_24658        4613 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHIS3NB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Meijer AH, Ouwerkerk PB, Hoge JH.
  TITLE     Vectors for transcription factor cloning and target site 
            identification by means of genetic selection in yeast
  JOURNAL   Yeast 14 (15), 1407-1415 (1998)
  PUBMED    9848232
REFERENCE   2  (bases 1 to 4613)
  AUTHORS   Ouwerkerk PB.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2000) Molecular Cell Biology, Institute of 
            Molecular Plant Sciences, Wassenaarseweg 64, Leiden 2333 AL, 
            Netherlands
REFERENCE   3  (bases 1 to 4613)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4613)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast"; 
            date: "1998"; volume: "14"; issue: "15"; pages: "1407-1415"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2000) Molecular Cell Biology, Institute of Molecular Plant 
            Sciences, Wassenaarseweg 64, Leiden 2333 AL, Netherlands"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4613
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      complement(950..1589)
                     /regulatory_class="terminator"
     CDS             complement(1593..2249)
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     primer_bind     complement(2372..2388)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2425..2443)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2464..2480)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2488..2504)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2512..2542)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2557..2578)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2866..3454)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3628..4485)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4486..4590)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 gccccccctc gaggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
      721 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
      781 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
      841 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
      901 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcctcggg
      961 gacaccaaat atggcgatct cggccttttc gtttcttgga gctgggacat gtttgccatc
     1021 gatccatcta ccaccagaac ggccgttaga tctgctgcca ccgttgtttc caccgaagaa
     1081 accaccgttg ccgtaaccac cacgacggtt gttgctaaag aagctgccac cgccacggcc
     1141 accgttgtag ccgccgttgt tgttattgta gttgctcatg ttatttctgg cacttcttgg
     1201 ttttcctctt aagtgaggag gaacataacc attctcgttg ttgtcgttga tgcttaaatt
     1261 ttgcacttgt tcgctcagtt cagccataat atgaaatgct tttcttgttg ttcttacgga
     1321 ataccacttg ccacctatca ccacaactaa ctttttcccg ttcctccatc tcttttatat
     1381 tttttttctc gagttcaaga gaaaaaaaaa gaaaaagcaa aaagaaaaaa ggaaagcgcg
     1441 cctcgttcag aatgacacgt atagaatgat gcattacctt gtcatcttca gtatcatact
     1501 gttcgtatac atacttactg acattcatag gtatacatat atacacatgt atatatatcg
     1561 tatgctgcag ctttaaataa tcggtgtcac tacataagaa cacctttggt ggagggaaca
     1621 tcgttggtac cattgggcga ggtggcttct cttatggcaa ccgcaagagc cttgaacgca
     1681 ctctcactac ggtgatgatc attcttgcct cgcagacaat caacgtggag ggtaattctg
     1741 ctagcctctg caaagctttc aagaaaatgc gggatcatct cgcaagagag atctcctact
     1801 ttctcccttt gcaaaccaag ttcgacaact gcgtacggcc tgttcgaaag atctaccacc
     1861 gctctggaaa gtgcctcatc caaaggcgca aatcctgatc caaacctttt tactccacgc
     1921 gccagtaggg cctctttaaa agcttgaccg agagcaatcc cgcagtcttc agtggtgtga
     1981 tggtcgtcta tgtgtaagtc accaatgcac tcaacgatta gcgaccagcc ggaatgcttg
     2041 gccagagcat gtatcatatg gtccagaaac cctatacctg tgtggacgtt aatcacttgc
     2101 gattgtgtgg cctgttctgc tactgcttct gcctcttttt ctgggaagat cgagtgctct
     2161 atcgctaggg gaccaccctt taaagagatc gcaatctgaa tcttggtttc atttgtaata
     2221 cgctttacta gggctttctg ctctgtcatc tttgccttcg tttatcttgc ctgctcattt
     2281 tttagtatat tcttcgaaga aatcacatta ctttatataa tgtataattc attatgtgat
     2341 aatgccagga attcctgcag cccgggggat cgatccacta gttctagagc ggccgccacc
     2401 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc
     2461 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg
     2521 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat
     2581 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
     2641 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct
     2701 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     2761 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     2821 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     2881 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     2941 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     3001 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     3061 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     3121 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     3181 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     3241 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     3301 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     3361 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     3421 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     3481 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     3541 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     3601 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     3661 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     3721 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     3781 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     3841 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     3901 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     3961 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     4021 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     4081 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     4141 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     4201 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     4261 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     4321 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     4381 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     4441 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     4501 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     4561 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac
//
