LOCUS       62056_12335        7474 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             18..35
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     rep_origin      complement(176..764)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(938..1795)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1796..1900)
                     /label=AmpR promoter
     CDS             complement(2067..3440)
                     /codon_start=1
                     /label=TcR
                     /note="tetracycline efflux protein"
                     /translation="VNTSYSQSNLRHNQILIWLCILSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFFS
                     LLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGMIA
                     HYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTTSYS
                     ISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPY
                     MMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTA
                     SFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGT
                     GIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF
                     "
     CDS             complement(3643..4644)
                     /codon_start=1
                     /label=repB
                     /note="RepB replication protein"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
                     FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
                     QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
                     GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
                     VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
                     YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
     promoter        5069..5097
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein
                     gene"
ORIGIN
        1 ccggcctgag aggatcgcat caccatcacc atcactaacc ggtccaagaa ttggagccaa
       61 tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc
      121 gtccgccatc tccagcagcc gcacgcggcg catctcgggc cgcgttgctg gcgtttttcc
      181 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      241 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      301 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      361 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      421 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      481 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      541 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      601 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      661 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      721 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      781 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      841 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      901 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      961 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     1021 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     1081 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     1141 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     1201 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg
     1261 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     1321 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     1381 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1441 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1501 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata
     1561 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1621 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1681 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1741 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1801 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1861 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1921 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca
     1981 cgaggccctt tcgtcttcaa gaattcctgt tataaaaaaa ggatcaattt tgaactctct
     2041 cccaaagttg atcccttaac gatttagaaa tccctttgag aatgtttata tacattcaag
     2101 gtaaccagcc aactaatgac aatgattcct gaaaaaagta ataacaaatt actatacaga
     2161 taagttgact gatcaacttc cataggtaac aacctttgat caagtaaggg tatggataat
     2221 aaaccaccta caattgcaat acctgttccc tctgataaaa agctggtaaa gttaagcaaa
     2281 ctcattccag caccagcttc ctgctgtttc aagctacttg aaacaattgt tgatataact
     2341 gttttggtga acgaaagccc acctaaaaca aatacgatta taattgtcat gaaccatgat
     2401 gttgtttcta aaagaaagga agcagttaaa aagctaacag aaagaaatgt aactccgatg
     2461 tttaacacgt ataaaggacc tcttctatca acaagtatcc caccaatgta gccgaaaata
     2521 atgacactca ttgttccagg gaaaataatt acacttccga tttcggcagt acttagctgg
     2581 tgaacatctt tcatcatata aggaaccata gagacaaacc ctgctactgt tccaaatata
     2641 attcccccac aaagaactcc aatcataaaa ggtatatttt tccctaatcc gggatcaaca
     2701 aaaggatctg ttactttcct gatatgtttt acaaatatca ggaatgacag cacgctaacg
     2761 ataagaaaag aaatgctata tgatgttgta aacaacataa aaaatacaat gcctacagac
     2821 attagtataa ttcctttgat atcaaaatga ccttttatcc ttacttcttt ctttaataat
     2881 ttcataagaa acggaacagt gataattgtt atcataggaa tgagtagaag ataggaccaa
     2941 tgaatataat gggctatcat tccaccaatc gctggaccga ctccttctcc catggctact
     3001 atcgatccaa taagaccaaa tgctttaccc ctattttcct ttggaatata gcgcgcaact
     3061 acaaccatta cgagtgctgg aaatgcagct gcaccagccc cttgaataaa acgagccata
     3121 ataagtaagg aaaagaaaga atggccaaca aacccaatta ccgacccgaa acaatttatt
     3181 ataattccaa ataggagtaa ccttttgatg cctaattgat cagatagctt tccatataca
     3241 gctgttccaa tggaaaaggt taacataaag gctgtgttca cccagtttgt actcgcaggt
     3301 ggtttattaa aatcatttgc aatatcaggt aatgagacgt tcaaaaccat ttcatttaat
     3361 acgctaaaaa aagataaaat gcaaagccaa attaaaattt ggttgtgtcg taaattcgat
     3421 tgtgaatagg atgtattcac atttcaccct ccaataatga gggcagacgt agtttatagg
     3481 gttaatgata cgcttccctc ttttaattga accctgttac attcattaca cttcataatt
     3541 aattcctcct aaacttgatt aaaacatttt accacatata aactaagttt taaattcagt
     3601 atttcatcac ttatacaaca atatggcccg tttgttgaac tactctttaa taaaataatt
     3661 tttccgttcc caattccaca ttgcaataat agaaaatcca tcttcatcgg ctttttcgtc
     3721 atcatctgta tgaatcaaat cgccttcttc tgtgtcatca aggtttaatt ttttatgtat
     3781 ttcttttaac aaaccaccat aggagattaa ccttttacgg tgtaaacctt cctccaaatc
     3841 agacaaacgt ttcaaattct tttcttcatc atcggtcata aaatccgtat cctttacagg
     3901 atattttgca gtttcgtcaa ttgccgattg tatatccgat ttatatttat ttttcggtcg
     3961 aatcatttga acttttacat ttggatcata gtctaatttc attgcctttt tccaaaattg
     4021 aatccattgt ttttgattca cgtagttttc tgtattctta aaataagttg gttccacaca
     4081 taccaataca tgcatgtgct gattataaga attatcttta ttatttattg tcacttccgt
     4141 tgcacgcata aaaccaacaa gatttttatt aattttttta tattgcatca ttcggcgaaa
     4201 tccttgagcc atatctgaca aactcttatt taattcttcg ccatcataaa catttttaac
     4261 tgttaatgtg agaaacaacc aacgaactgt tggcttttgt ttaataactt cagcaacaac
     4321 cttttgtgac tgaatgccat gtttcattgc tctcctccag ttgcacattg gacaaagcct
     4381 ggatttacaa aaccacactc gatacaactt tctttcgcct gtttcacgat tttgtttata
     4441 ctctaatatt tcagcacaat cttttactct ttcagccttt ttaaattcaa gaatatgcag
     4501 aagttcaaag taatcaacat tagcgatttt cttttctctc catggtctca cttttccact
     4561 ttttgtcttg tccactaaaa cccttgattt ttcatctgaa taaatgctac tattaggaca
     4621 cataatatta aaagaaaccc ccatctattt agttatttgt ttggtcactt ataactttaa
     4681 cagatggggt ttttctgtgc aaccaatttt aagggttttc aatactttaa aacacataca
     4741 taccaacact tcaacgcacc tttcagcaac taaaataaaa atgacgttat ttctatatgt
     4801 atcaagataa gaaagaacaa gttcaaaacc atcaaaaaaa gacacctttt caggtgcttt
     4861 ttttatttta taaactcatt ccctgatctc gacttcgttc tttttttacc tctcggttat
     4921 gagttagttc aaattcgttc tttttaggtt ctaaatcgtg tttttcttgg aattgtgctg
     4981 ttttatcctt taccttgtct acaaacccct taaaaacgtt tttaaaggct tttaagccgt
     5041 ctgtacgttc cttaaggaat tctcatgttt gacagcttat catcgataag ctttaatgcg
     5101 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
     5161 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
     5221 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
     5281 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
     5341 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
     5401 actacgcgat catggcgacc acacccgtcc tgtggatctg acgcgtgtaa ctgcgaaggt
     5461 aaaacgggtg attgaaaata aactaacaaa tgaagaggaa cgaaagagaa gtctagaatt
     5521 tgttacgttt atatcggatg aattactgca aaatgatacg gagcctcgca catttattat
     5581 tcagaactct cttttatcgc tagagaaaat ccatactctc gcacagacca aagaggtaga
     5641 ggaatataaa gaagtcataa ctactctgac aagacatgat tcataatttg aaaagcaggt
     5701 aaactaaccc aaaaggcaag actctccgta gtttagaaaa gagcaggttg ctaataacat
     5761 ataaacagcc agttgccgtt atgataggtg actggctgca tgggatgaaa aggtgagggt
     5821 ggagacagac ataacactct taatagaaga gggtaattct ttctctttta tagaaaatca
     5881 attaattgaa agtagctcct tcattcttaa gatcaacgtg atataggttt gctaaccttt
     5941 gcgttcactt aactaactta taggggtaac acttaaaaaa gaatcaataa cgatagaaac
     6001 cgctcctaaa gcaggtgcat tttttcctaa cgaagaaggc aatagttcac atttattgtc
     6061 taaatgagaa tggactctag aagaaacttc gtttttaatc gtatttaaaa caatgggatg
     6121 agattcaatt atatgatttc tcaagataac agcttctata tcaaatgtat taaggatatt
     6181 ggttaatcca attccgatat aaaagccaaa gttttgaagt gcatttaaca tttctacatc
     6241 atttttattt gcgcgttcca caatctcttt tcgagaaata ttcttttctt ctttagagag
     6301 cgaagccagt aacgcttttt cagaagcata taattcccaa cagcctcgat ttccacagct
     6361 gcatttgggt ccattaaaat ctatcgtcat atgacccatt tccccagaaa aaccctgaac
     6421 acctttatac aattcgttgt taataacaag tccagttcca attccgatat taatactgat
     6481 gtaaacgatg ttttcatagt tttttgtcat accaaatact ttttcaccgt atgctcctgc
     6541 attagcttca ttttcaacaa aaaccggaac attaaactca ctctcaatta aaaactgcaa
     6601 atctttgata ttccaattta agttaggcat gaaaataatt tgctgatgac gatctacaag
     6661 gcctggaaca caaattccta ttccgactag accataaggg gactcaggca tatgggttac
     6721 aaaaccatga ataagtgcaa ataaaatctc ttttacttca ctagcggaag aactagacaa
     6781 gtcagaagtc ttctcgagaa taatatttcc ttctaagtcg gttagaattc cgttaagata
     6841 gtcgactcct atatcaatac caatcgagta gcctgcattc ttattaaaaa caagcattac
     6901 aggtcttctg ccgcctctag attgccctgc cccaatttca aaaataaaat ctttttcaag
     6961 cagtgtattt acttgagagg agacagtaga cttgtttaat cctgtaatct cagagagagt
     7021 tgccctggag acaggggagt tcttcaaaat ttcatctaat attaattttt gattcatttt
     7081 ttttactaaa gcttgatctg caatttgaat aataaccact cctttgttta tccaccgaac
     7141 taagttggtg ttttttgaag cttgaattag atatttaaaa gtatcatatc taatattata
     7201 actaaatttt ctaaaaaaaa cattgaaata aacatttatt ttgtatatga tgagataaag
     7261 ttagtttatt ggataaacaa actaactcaa ttaagatagt tgatggataa acttgttcac
     7321 ttaaatcaaa gggggaaatg tacaatgaaa aaagtactta tggcattcat tatttgttta
     7381 tcgctgattc tatctgtttt agccgctccg ccgtctggcg caggcgccgc attgaagatc
     7441 tccggagctc ccgggatccg gtaccggccg catg
//
