LOCUS       40924_24573        3656 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHIND2.2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3656)
  AUTHORS   McNulty JJ, Dunn JJ, Randesi M.
  TITLE     Development of the pHIND series of plasmids useful for generating 
            nested deletions
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3656)
  AUTHORS   McNulty JJ, Dunn JJ, Randesi M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-1999) Biology, Brookhaven National Laboratory, 50 
            Bell Ave., Upton, NY 11973, USA
REFERENCE   3  (bases 1 to 3656)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3656)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-FEB-1999) Biology, Brookhaven National Laboratory, 50 Bell Ave.,
            Upton, NY 11973, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    68..89
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        104..134
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    142..158
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     166..182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        200..218
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     misc_feature    complement(225..281)
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(284..302)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(309..325)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(622..1434)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(1736..2324)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2498..3355)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3356..3460)
                     /label=AmpR promoter
     primer_bind     3557..3573
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      3592..3642
                     /note="F1 phage; used for specifically nicking DNA"
ORIGIN
        1 acgcgtggcg cgccttaatt aatcgcgtgc ggccggactg gaaagcgggc agtgagcgca
       61 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
      121 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
      181 accatgatta cgccaagcta tttaggtgac actatagaat actcaagctt gcatgcctgc
      241 aggtcgactc tagaggatcc ccgggtaccg agctcgaatt cgccctatag tgagtcgtat
      301 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      361 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      421 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggacgcg ccctgtagcg
      481 gcgcattaag cggccgcgtt tgtttaaaca tttaaatgcc cgggccctgc aggccggcca
      541 aactaactat aacggtccta aggtagcgac ggccgcgatc cgagctcgcc agtgttacaa
      601 ccaattaacc aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
      661 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
      721 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
      781 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
      841 atcaccatga gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca
      901 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
      961 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
     1021 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     1081 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     1141 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     1201 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     1261 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     1321 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     1381 gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt tgaatatggc tcataacacc
     1441 ccttngtatt actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat
     1501 cttgtgcaat gtaacatcag agattttgag acacaacgtg gctttgctag cggatcgcgg
     1561 cctcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     1621 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     1681 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcg
     1741 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     1801 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     1861 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     1921 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     1981 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     2041 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     2101 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     2161 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     2221 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     2281 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     2341 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     2401 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     2461 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     2521 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     2581 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     2641 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     2701 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     2761 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tggcattgct acaggcatcg
     2821 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     2881 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     2941 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     3001 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     3061 cattctgaga atagagtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     3121 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     3181 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     3241 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     3301 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     3361 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     3421 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     3481 cacctgctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     3541 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg ccggatcgat ccgagtgttg
     3601 ttccagtttg gaacaagagt ccactattaa agaacgtgga cacgtgatcg atgcat
//
