LOCUS       62056_12315        8424 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8424)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8424
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(774..792)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      complement(1050..1638)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1812..2669)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2670..2774)
                     /label=AmpR promoter
     promoter        3120..3138
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     enhancer        4526..4905
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4906..5109
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     promoter        5154..5172
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7384..8091
                     /codon_start=1
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     promoter        complement(8134..8152)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     polyA_signal    8178..8402
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 atccgtgtaa ccgccagtcc gtgcttgcct agcgaactca cccgtcccgg ctggcgtgcg
       61 cagcccgggc cgtgttgcgg gccctcttaa ggggcggcgg caggacgggg actcccgccc
      121 cgcctctttt cccccgggga gtcaaccccc ggggggggtg ttttttgggg gggggcgcga
      181 aggcgggcgg cggcggcggg cgggcggcag ggcagccccg cgcgccccct tccccgtccc
      241 tcccccggag ccggccgctc ccccgcgggc gccgcccctc cccccgcgcg ccgcggggct
      301 gccttcccgc gggcgccccc gcgcggcttt tttcccgcgc ccgcccccgc gcggcaggac
      361 ggggactagc aggctgtgcc gcagaccacc acacactccc aagctccccg cccccccgaa
      421 gacgccagtc gcaccaccgc tcgccctcgc agaccagaca gttgcaccaa gcacccgccc
      481 gcccgcacac ggttccccgc caccccctcc ctcccctcca tcccgccgag ctcgcggcag
      541 cccctccccc ccgcgcgcca cggggctgcg gtcccgcggc cgcctccccc gcggccgcct
      601 cccccgcgcc ccgccccggg ggcttccccc gcccctcccc ccgcgcccgc ggccccgagc
      661 tcgcagcagc ccctccctcc cgcgccccgt gccttccctc ccgctcctgc gggggggctc
      721 gggccacctg accttcgtaa cctgcactca ggtcagagag atctgccggt ctccctatag
      781 tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcgg ccaacgcgcg
      841 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
      901 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
      961 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     1021 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     1081 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     1141 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     1201 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg
     1261 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     1321 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     1381 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     1441 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt
     1501 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     1561 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     1621 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     1681 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     1741 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     1801 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     1861 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     1921 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     1981 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     2041 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     2101 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     2161 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     2221 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     2281 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     2341 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     2401 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     2461 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     2521 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     2581 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     2641 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     2701 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     2761 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     2821 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     2881 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg
     2941 taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt
     3001 cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatggac
     3061 atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat acacatacga
     3121 tttaggtgac actatagaac tcgagcagct gcggtgaagg tcgtcgtcag agattcccac
     3181 ctcggtctcc tcctccgagt cgctgctggc gagccactgc atgtcgttga gcatccccca
     3241 ggcgtgcggg gcggcgggct gcttgacaaa gcaacggggg ggatttagag ggcgcggggc
     3301 gtgaggcggg acccccgcgc cgtgtccccc gtgtccctcc ctcaccccgg ccccccgccc
     3361 gctgcttttt gttcggaagg gggggagaaa ggggtccgta accaaaggtg gtctgcgtcc
     3421 tttggattcc gacccctcgt ctccccccct gtcccccgct ctcgggctcc tccctgcctc
     3481 cctcgccccc ccagagggtc ggggggcggc gcacggccca cgggggtccc ccgaccgctt
     3541 aagcgggccg ggggtcggcc ccgtcaagcg tccccgcccc cgagcccacc gcccgcgacc
     3601 acccccaacc cgcagccggg tggtccgggg aaaagggggg gcctgagacc cgggggtcgc
     3661 cctctcaccg tgccgggggt ctgccgcggc ggccgctcgg ggccggggtc cgcccgggag
     3721 ctcgtgccgg gccggggttc catgagccgg ggtagggtag actcgagacg gcggcccgcg
     3781 gtctctctct tgccgggttt tagtctctgt ctctccgggt ctcctcctcc cgccgggccg
     3841 ccgctccgtc gctcgcagtg ccggggtgcg aatgcggccc gaccgtcaca cggggctgcc
     3901 ttatacccgg cgcctatcca ctcccccaaa ggggcggcat ttacgattcc cccaatagcc
     3961 gcgcgccccg gcgggggcgg agggagggaa tccccccctc tcggggcggc cccgtccccg
     4021 gggaccaacc gggtgtactc caagaacccc attagcatgc gccgcccccc gccgacgcag
     4081 atgggagtcc ccccggcgcc ccgccggcgc ggccctgagt ggtgcccgcc cccggggaaa
     4141 aattcattag catactagga agcccagggg accaataggg gccgatcagc ccacccaccc
     4201 ggcggcgcgc gaggctctgc gtgttctgcc aagaaagtaa tcagcataac ccggaacccc
     4261 gagggagtaa ttacgcgggg agcgaggggc cgtccgaacg tttttaatta ccataagcgg
     4321 gaatggcggc ccgttaaaag ctgctaatta ccgcgagcgg gaacgccggc ccattaaaag
     4381 ttgctaatta ccatgcgcgg ggatggcggc cgggaccgcc tattaaaagt ttctaattac
     4441 cataccggga agccggctct agaggatccc cgggtaccga gctcgaattc atcgatgatc
     4501 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     4561 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     4621 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     4681 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga ctatttacgg
     4741 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     4801 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     4861 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     4921 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     4981 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     5041 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     5101 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
     5161 actcactata gggagaccca agcttggtac cgagctcgga tccactagta acggccgcca
     5221 gtgtgctgga attctgcaga tatcatgcag gtgcagctgg tgcagagcgg cgtggaggtg
     5281 aagaagcccg gcgccagcgt gaaggtgagc tgcaaggcca gcggctacac cttcaccaac
     5341 tactacatgt actgggtgcg gcaggccccc ggccagggcc tggagtggat gggcggcatc
     5401 aaccccagca acggcggcac caacttcaac gagaagttca agaaccgggt gaccctgacc
     5461 accgacagca gcaccaccac cgcctacatg gagctgaaga gcctgcagtt cgacgacacc
     5521 gccgtgtact actgcgcccg gcgggactac cggttcgaca tgggcttcga ctactggggc
     5581 cagggcacca ccgtgaccgt gagcagcgcc agcaccaagg gccccagcgt gttccccctg
     5641 gccccctgca gccggagcac cagcgagagc accgccgccc tgggctgcct ggtgaaggac
     5701 tacttccccg agcccgtgac cgtgagctgg aacagcggcg ccctgaccag cggcgtgcac
     5761 accttccccg ccgtgctgca gagcagcggc ctgtacagcc tgagcagcgt ggtgaccgtg
     5821 cccagcagca gcctgggcac caagacctac acctgcaacg tggaccacaa gcccagcaac
     5881 accaaggtgg acaagcgggt ggagagcaag tacggccccc cctgcccccc ctgccccgcc
     5941 cccgagttcc tgggcggccc cagcgtgttc ctgttccccc ccaagcccaa ggacaccctg
     6001 atgatcagcc ggacccccga ggtgacctgc gtggtggtgg acgtgagcca ggaggacccc
     6061 gaggtgcagt tcaactggta cgtggacggc gtggaggtgc acaacgccaa gaccaagccc
     6121 cgggaggagc agttcaacag cacctaccgg gtggtgagcg tgctgaccgt gctgcaccag
     6181 gactggctga acggcaagga gtacaagtgc aaggtgagca acaagggcct gcccagcagc
     6241 atcgagaaga ccatcagcaa ggccaagggc cagccccggg agccccaggt gtacaccctg
     6301 ccccccagcc aggaggagat gaccaagaac caggtgagcc tgacctgcct ggtgaagggc
     6361 ttctacccca gcgacatcgc cgtggagtgg gagagcaacg gccagcccga gaacaactac
     6421 aagaccaccc cccccgtgct ggacagcgac ggcagcttct tcctgtacag ccggctgacc
     6481 gtggacaaga gccggtggca ggagggcaac gtgttcagct gcagcgtgat gcacgaggcc
     6541 ctgcacaacc actacaccca gaagagcctg agcctgagcc tgggcaaggg cagcggcgcc
     6601 accaacttca gcctgctgaa gcaggccggc gacgtggagg agaaccccgg ccccgagatc
     6661 gtgctgaccc agagccccgc caccctgagc ctgagccccg gcgagcgggc caccctgagc
     6721 tgccgggcca gcaagggcgt gagcaccagc ggctacagct acctgcactg gtaccagcag
     6781 aagcccggcc aggccccccg gctgctgatc tacctggcca gctacctgga gagcggcgtg
     6841 cccgcccggt tcagcggcag cggcagcggc accgacttca ccctgaccat cagcagcctg
     6901 gagcccgagg acttcgccgt gtactactgc cagcacagcc gggacctgcc cctgaccttc
     6961 ggcggcggca ccaaggtgga gatcaagcgg accgtggccg cccccagcgt gttcatcttc
     7021 ccccccagcg acgagcagct gaagagcggc accgccagcg tggtgtgcct gctgaacaac
     7081 ttctaccccc gggaggccaa ggtgcagtgg aaggtggaca acgccctgca gagcggcaac
     7141 agccaggaga gcgtgaccga gcaggacagc aaggacagca cctacagcct gagcagcacc
     7201 ctgaccctga gcaaggccga ctacgagaag cacaaggtgt acgcctgcga ggtgacccac
     7261 cagggcctga gcagccccgt gaccaagagc ttcaaccggg gcgagtgcga gggcagagga
     7321 agtcttctaa catgcggtga cgtggaggag aatcccggcc ctccggatcc accggtcgcc
     7381 accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc
     7441 aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag
     7501 ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg
     7561 cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag
     7621 caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag
     7681 cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag
     7741 gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc
     7801 gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac
     7861 ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac
     7921 gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac
     7981 gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac
     8041 gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa gtagagcggc
     8101 cggccgctcg agcatgcatc tagagggccc tattctatag tgtcacctaa atgctagagc
     8161 tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     8221 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     8281 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
     8341 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
     8401 ggcttctgag gcggaaagaa ccag
//
