LOCUS       62056_12305        4837 bp DNA     circular SYN 13-FEB-2019
DEFINITION  Cloning vector pHEN2-rED3_301, complete sequence.
ACCESSION   MH319019
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Baykov IK, Emelyanova LA, Sokolova LM, Karelina EM, Matveev AL, 
            Babkin IV, Khlusevich Y, Podgornyy VF, Tikunova NV.
  TITLE     ANALYSIS OF DOMAIN SPECIFICITY OF THE PROTECTIVE CHIMERIC ANTIBODY 
            ch14D5a AGAINST GLYCOPROTEIN E OF TICK-BORNE ENCEPHALITIS VIRUS
  JOURNAL   Vavilovskii Zhurnal Genet Selektsii 22 (4), 459-467 (2018)
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Baykov IK, Tikunova NV.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2018) Laboratory of Molecular Microbiology, 
            Institute of Chemical Biology and Fundamental Medicine SB RAS, 
            Lavrentieva 8, Novosibirks 630090, Russia
REFERENCE   3  (bases 1 to 4837)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Vavilovskii
            Zhurnal Genet Selektsii"; date: "2018"; volume: "22"; issue: "4"; 
            pages: "459-467"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (08-MAY-2018) Laboratory of Molecular Microbiology, Institute of 
            Chemical Biology and Fundamental Medicine SB RAS, Lavrentieva 8, 
            Novosibirks 630090, Russia"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     sig_peptide     2274..2339
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     CDS             2643..2660
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             2673..2690
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             2700..2729
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             2745..3956
                     /codon_start=1
                     /label=M13 gene III
                     /note="pIII"
                     /translation="TVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVVVCT
                     GDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTYINPL
                     DGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTDPVKTY
                     YQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAGGGSGGG
                     SGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENADENALQSD
                     AKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPLMNNFR
                     QYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMYVFSTFANIL
                     RNKES"
     primer_bind     complement(3969..3985)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      complement(4181..4636)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgccaagctt gcatgcaaat tctatttcaa ggagacagtc ataatgaaat
     2281 acctattgcc tacggcagcc gctggattgt tattactcgc ggcccagccg gccatggccg
     2341 gcggtggctc gggtcttaca tacacaatgt gcgacaagac gaaattcacg tggaaaagaa
     2401 ttccaacaga cagtggacat gacacagtgg tcatggaagt tgcgttctct gggaccaaac
     2461 cctgcaggat cccggtgagg gccgtggcac acggctcccc ggatgtgaac gtggccatgt
     2521 tgataacacc caaccccaca atcgaaaaca atggcggtgg cttcatagaa atgcagttac
     2581 ctccaggaga taatatcatc tatgttgggg aactgagtca ccaatggttc caaaaaggga
     2641 gtcatcatca tcaccatcac taagcggccg cacatcatca tcaccatcac ggggccgcag
     2701 aacaaaaact catctcagaa gaggatctga atggggccgc atagactgtt gaaagttgtt
     2761 tagcaaaacc tcatacagaa aattcattta ctaacgtctg gaaagacgac aaaactttag
     2821 atcgttacgc taactatgag ggctgtctgt ggaatgctac aggcgttgtg gtttgtactg
     2881 gtgacgaaac tcagtgttac ggtacatggg ttcctattgg gcttgctatc cctgaaaatg
     2941 agggtggtgg ctctgagggt ggcggttctg agggtggcgg ttctgagggt ggcggtacta
     3001 aacctcctga gtacggtgat acacctattc cgggctatac ttatatcaac cctctcgacg
     3061 gcacttatcc gcctggtact gagcaaaacc ccgctaatcc taatccttct cttgaggagt
     3121 ctcagcctct taatactttc atgtttcaga ataataggtt ccgaaatagg cagggtgcat
     3181 taactgttta tacgggcact gttactcaag gcactgaccc cgttaaaact tattaccagt
     3241 acactcctgt atcatcaaaa gccatgtatg acgcttactg gaacggtaaa ttcagagact
     3301 gcgctttcca ttctggcttt aatgaggatc cattcgtttg tgaatatcaa ggccaatcgt
     3361 ctgacctgcc tcaacctcct gtcaatgctg gcggcggctc tggtggtggt tctggtggcg
     3421 gctctgaggg tggcggctct gagggtggcg gttctgaggg tggcggctct gagggtggcg
     3481 gttccggtgg cggctccggt tccggtgatt ttgattatga aaaaatggca aacgctaata
     3541 agggggctat gaccgaaaat gccgatgaaa acgcgctaca gtctgacgct aaaggcaaac
     3601 ttgattctgt cgctactgat tacggtgctg ctatcgatgg tttcattggt gacgtttccg
     3661 gccttgctaa tggtaatggt gctactggtg attttgctgg ctctaattcc caaatggctc
     3721 aagtcggtga cggtgataat tcacctttaa tgaataattt ccgtcaatat ttaccttctt
     3781 tgcctcagtc ggttgaatgt cgcccttatg tctttggcgc tggtaaacca tatgaatttt
     3841 ctattgattg tgacaaaata aacttattcc gtggtgtctt tgcgtttctt ttatatgttg
     3901 ccacctttat gtatgtattt tcgacgtttg ctaacatact gcgtaataag gagtcttaat
     3961 aagaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     4021 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     4081 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     4141 tttctcctta cgcatctgtg cggtatttca caccgcatat aaattgtaaa cgttaatatt
     4201 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     4261 atcggcaaaa tcccttataa atcaaaagaa tagcccgaga tagggttgag tgttgttcca
     4321 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     4381 gtctatcagg gcgatggccc actacgtgaa ccatcaccca aatcaagttt tttggggtcg
     4441 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     4501 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     4561 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     4621 ccgctacagg gcgcgtacta tggttgcttt gacgggtgca ctctcagtac aatctgctct
     4681 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     4741 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     4801 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga
//
