LOCUS       pHELLSGATE.       18691 bp DNA     circular SYN 01-JAN-1980
DEFINITION  High-throughput binary Gateway(R) donor vector for silencing genes 
            in plants using intron-containing hairpin RNA.
ACCESSION   .
VERSION     .
KEYWORDS    pHELLSGATE
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18691)
  AUTHORS   Wesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, Liu Q, Gooding
            PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP, 
            Green AG, Waterhouse PM.
  TITLE     Construct design for efficient, effective and high-throughput gene 
            silencing in plants.
  JOURNAL   Plant J. 2001;27:581-90.
  PUBMED    11576441
REFERENCE   2  (bases 1 to 18691)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 18691)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2001"; volume: "27"; pages: "581-90"
COMMENT     SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..18691
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(65..83)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(90..106)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        264..447
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             448..1266
                     /label=KanR
                     /note="fusion between an N-terminal peptide of nopaline
                     synthase and Tn5 aminoglycoside phosphotransferase"
     terminator      1894..2146
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    complement(2268..2292)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     oriT            2846..2955
                     /label=oriT
                     /note="incP origin of transfer"
     mobile_element  3015..3782
                     /label=IS1
                     /note="prokaryotic transposable element"
     CDS             3782..4132
                     /label=traJ
                     /note="oriT-recognizing protein"
     CDS             4407..5552
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
     rep_origin      complement(6915..7503)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             8600..9388
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase"
                     /label=aadA
                     /note="SmR"
                     /note="confers resistance to spectinomycin and
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      complement(9993..10705)
                     /direction=LEFT
                     /label=oriV
                     /note="incP origin of replication"
     misc_feature    complement(11182..11206)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    11462..11483
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        11498..11528
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11536..11552
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11560..11576
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11594..11612
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        12672..13017
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    13048..13279
                     /label=attP1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(13678..13980)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(14387..14618)
                     /label=attP2
                     /note="recombination site for the Gateway(R) BP reaction 
                     (pDONR(TM)221 version)"
     intron          14662..16258
                     /label=PDK intron
                     /note="modified pyruvate orthophosphate dikinase intron
                     from Flaveria trinervia"
     protein_bind    16320..16551
                     /label=attP2
                     /note="recombination site for the Gateway(R) BP reaction 
                     (pDONR(TM)221 version)"
     CDS             complement(16858..17160)
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(17659..17890)
                     /label=attP1
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      17922..18628
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     promoter        complement(18669..18687)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 ggccgcacta gtgatatccc gcggccatgg cggccgggag catgcgacgt cgggcccaat
       61 tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg tcgtgactgg
      121 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      181 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      241 gaatggaaat tgtaaacgtt aatgggtttc tggagtttaa tgagctaagc acatacgtca
      301 gaaaccatta ttgcgcgttc aaaagtcgcc taaggtcact atcagctagc aaatatttct
      361 tgtcaaaaat gctccactga cgttccataa attcccctcg gtatccaatt agagtctcat
      421 attcactctc aatccaaata atctgcaatg gcaattacct tatccgcaac ttctttacct
      481 atttccgccc ggatccgggc aggttctccg gccgcttggg tggagaggct attcggctat
      541 gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag
      601 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac
      661 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac
      721 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc
      781 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg
      841 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag
      901 cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat
      961 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag
     1021 gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc
     1081 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg
     1141 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg
     1201 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag
     1261 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat
     1321 cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc
     1381 gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc
     1441 ccgatccaac acttacgttt gcaacgtcca agagcaaata gaccacgaac gccggaaggt
     1501 tgccgcagcg tgtggattgc gtctcaattc tctcttgcag gaatgcaatg atgaatatga
     1561 tactgactat gaaactttga gggaatactg cctagcaccg tcacctcata acgtgcatca
     1621 tgcatgccct gacaacatgg aacatcgcta tttttctgaa gaattatgct cgttggagga
     1681 tgtcgcggca attgcagcta ttgccaacat cgaactaccc ctcacgcatg cattcatcaa
     1741 tattattcat gcggggaaag gcaagattaa tccaactggc aaatcatcca gcgtgattgg
     1801 taacttcagt tccagcgact tgattcgttt tggtgctacc cacgttttca ataaggacga
     1861 gatggtggag taaagaagga gtgcgtcgaa gcagatcgtt caaacatttg gcaataaagt
     1921 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     1981 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     2041 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     2101 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcgaat taattccagg
     2161 cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accccagtac
     2221 attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt tacaccacaa
     2281 tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac aaaatcacca
     2341 ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt tgtaaggcgg
     2401 cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct gccgggtttg
     2461 aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag agcgttgctg
     2521 cctgtgatca aatatcatct ccctcgcaga gatccgaatt atcagccttc ttattcattt
     2581 ctcgcttaac cgtgacaggc tgtcgatctt gagaactatg ccgacataat aggaaatcgc
     2641 tggataaagc cgctgaggaa gctgagtggc gctatttctt tagaagtgaa cgttgacgat
     2701 gtcgacggat cttttccgct gcataaccct gcttcggggt cattatagcg attttttcgg
     2761 tatatccatc ctttttcgca cgatatacag gattttgcca aagggttcgt gtagactttc
     2821 cttggtgtat ccaacggcgt cagccgggca ggataggtga agtaggccca cccgcgagcg
     2881 ggtgttcctt cttcactgtc ccttattcgc acctggcggt gctcaacggg aatcctgctc
     2941 tgcgaggctg gccggctacc gccggcgtaa cagatgaggg caagcggatg gctgatgaaa
     3001 ccaagccaac caggggtgat gctgccaact tactgattta gtgtatgatg gtgtttttga
     3061 ggtgctccag tggcttctgt ttctatcagc tgtccctcct gttcagctac tgacggggtg
     3121 gtgcgtaacg gcaaaagcac cgccggacat cagcgctatc tctgctctca ctgccgtaaa
     3181 acatggcaac tgcagttcac ttacaccgct tctcaacccg gtacgcacca gaaaatcatt
     3241 gatatggcca tgaatggcgt tggatgccgg gcaacagccc gcattatggg cgttggcctc
     3301 aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc
     3361 cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat gtcggggcta
     3421 aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg gttgttgcgc
     3481 acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct
     3541 ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa
     3601 agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga
     3661 ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc
     3721 atgacaaagt catcgggcat tatctgaaca taaaacacta tcaataagtt ggagtcatta
     3781 cccaaccagg aagggcagcc cacctatcaa ggtgtactgc cttccagacg aacgaagagc
     3841 gattgaggaa aaggcggcgg cggccggcat gagcctgtcg gcctacctgc tggccgtcgg
     3901 ccagggctac aaaatcacgg gcgtcgtgga ctatgagcac gtccgcgagc tggcccgcat
     3961 caatggcgac ctgggccgcc tgggcggcct gctgaaactc tggctcaccg acgacccgcg
     4021 cacggcgcgg ttcggtgatg ccacgatcct cgccctgctg gcgaagatcg aagagaagca
     4081 ggacgagctt ggcaaggtca tgatgggcgt ggtccgcccg agggcagagc catgactttt
     4141 ttagccgcta aaacggccgg ggggtgcgcg tgattgccaa gcacgtcccc atgcgctcca
     4201 tcaagaagag cgacttcgcg gagctggtat tcgtgcaggg caagattcgg aataccaagt
     4261 acgagaagga cggccagacg gtctacggga ccgacttcat tgccgataag gtggattatc
     4321 tggacaccaa ggcaccaggc gggtcaaatc aggaataagg gcacattgcc ccggcgtgag
     4381 tcggggcaat cccgcaagga gggtgaatga atcggacgtt tgaccggaag gcatacaggc
     4441 aagaactgat cgacgcgggg ttttccgccg aggatgccga aaccatcgca agccgcaccg
     4501 tcatgcgtgc gccccgcgaa accttccagt ccgtcggctc gatggtccag caagctacgg
     4561 ccaagatcga gcgcgacagc gtgcaactgg ctccccctgc cctgcccgcg ccatcggccg
     4621 ccgtggagcg ttcgcgtcgt ctcgaacagg aggcggcagg tttggcgaag tcgatgacca
     4681 tcgacacgcg aggaactatg acgaccaaga agcgaaaaac cgccggcgag gacctggcaa
     4741 aacaggtcag cgaggccaag caggccgcgt tgctgaaaca cacgaagcag cagatcaagg
     4801 aaatgcagct ttccttgttc gatattgcgc cgtggccgga cacgatgcga gcgatgccaa
     4861 acgacacggc ccgctctgcc ctgttcacca cgcgcaacaa gaaaatcccg cgcgaggcgc
     4921 tgcaaaacaa ggtcattttc cacgtcaaca aggacgtgaa gatcacctac accggcgtcg
     4981 agctgcgggc cgacgatgac gaactggtgt ggcagcaggt gttggagtac gcgaagcgca
     5041 cccctatcgg cgagccgatc accttcacgt tctacgagct ttgccaggac ctgggctggt
     5101 cgatcaatgg ccggtattac acgaaggccg aggaatgcct gtcgcgccta caggcgacgg
     5161 cgatgggctt cacgtccgac cgcgttgggc acctggaatc ggtgtcgctg ctgcaccgct
     5221 tccgcgtcct ggaccgtggc aagaaaacgt cccgttgcca ggtcctgatc gacgaggaaa
     5281 tcgtcgtgct gtttgctggc gaccactaca cgaaattcat atgggagaag taccgcaagc
     5341 tgtcgccgac ggcccgacgg atgttcgact atttcagctc gcaccgggag ccgtacccgc
     5401 tcaagctgga aaccttccgc ctcatgtgcg gatcggattc cacccgcgtg aagaagtggc
     5461 gcgagcaggt cggcgaagcc tgcgaagagt tgcgaggcag cggcctggtg gaacacgcct
     5521 gggtcaatga tgacctggtg cattgcaaac gctagggcct tgtggggtca gttccggctg
     5581 ggggttcagc agccagcgct ttactggcat ttcaggaaca agcgggcact gctcgacgca
     5641 cttgcttcgc tcagtatcgc tcgggacgca cggcgcgctc tacgaactgc cgataaacag
     5701 aggattaaaa ttgacaattg tgattaaggc tcagattcga cggcttggag cggccgacgt
     5761 gcaggatttc cgcgagatcc gattgtcggc cctgaagaaa gctccagaga tgttcgggtc
     5821 cgtttacgag cacgaggaga aaaagcccat ggaggcgttc gctgaacggt tgcgagatgc
     5881 cgtggcattc ggcgcctaca tcgacggcga gatcattggg ctgtcggtct tcaaacagga
     5941 ggacggcccc aaggacgctc acaaggcgca tctgtccggc gttttcgtgg agcccgaaca
     6001 gcgaggccga ggggtcgccg gtatgctgct gcgggcgttg ccggcgggtt tattgctcgt
     6061 gatgatcgtc cgacagattc caacgggaat ctggtggatg cgcatcttca tcctcggcgc
     6121 acttaatatt tcgctattct ggagcttgtt gtttatttcg gtctaccgcc tgccgggcgg
     6181 ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc gtgttcatct ctgccgctct
     6241 gctaggtagc ccgatacgat tgatggcggt cctgggggct atttgcggaa ctgcgggcgt
     6301 ggcgctgttg gtgttgacac caaacgcagc gctagatcct gtcggcgtcg cagcgggcct
     6361 ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc cgcaagtggc aacctcccgt
     6421 gcctctgctc acctttaccg cctggcaact ggcggccgga ggacttctgc tcgttccagt
     6481 agctttagtg tttgatccgc caatcccgat gcctacagga accaatgttc tcggcctggc
     6541 gtggctcggc ctgatcggag cgggtttaac ctacttcctt tggttccggg ggatctcgcg
     6601 actcgaacct acagttgttt ccttactggg ctttctcagc cgggatggcg ctaagaagct
     6661 attgccgccg atcttcatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     6721 cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     6781 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     6841 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6901 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6961 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     7021 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     7081 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     7141 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     7201 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     7261 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     7321 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     7381 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     7441 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatat
     7501 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7561 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7621 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7681 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     7741 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     7801 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     7861 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7921 aaacaagtgg cagcaacgga ttcgcaaacc tgtcacgcct tttgtgccaa aagccgcgcc
     7981 aggtttgcga tccgctgtgc caggcgttag gcgtcatatg aagatttcgg tgatccctga
     8041 gcaggtggcg gaaacattgg atgctgagaa ccatttcatt gttcgtgaag tgttcgatgt
     8101 gcacctatcc gaccaaggct ttgaactatc taccagaagt gtgagcccct accggaagga
     8161 ttacatctcg gatgatgact ctgatgaaga ctctgcttgc tatggcgcat tcatcgacca
     8221 agagcttgtc gggaagattg aactcaactc aacatggaac gatctagcct ctatcgaaca
     8281 cattgttgtg tcgcacacgc accgaggcaa aggagtcgcg cacagtctca tcgaatttgc
     8341 gaaaaagtgg gcactaagca gacagctcct tggcatacga ttagagacac aaacgaacaa
     8401 tgtacctgcc tgcaatttgt acgcaaaatg tggctttact ctcggcggca ttgacctgtt
     8461 cacgtataaa actagacctc aagtctcgaa cgaaacagcg atgtactggt actggttctc
     8521 gggagcacag gatgacgcct aacaattcat tcaagccgac accgcttcgc ggcgcggctt
     8581 aattcaggag ttaaacatca tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc
     8641 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     8701 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     8761 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     8821 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     8881 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     8941 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     9001 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     9061 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     9121 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     9181 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     9241 gcgcctgccg gcccagtatc agcccgtcat acttgaagct aggcaggctt atcttggaca
     9301 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgttcact acgtgaaagg
     9361 cgagatcacc aaggtagtcg gcaaataatg tctaacaatt cgttcaagcc gacgccgctt
     9421 cgcggcgcgg cttaactcaa gcgttagaga gctggggaag actatgcgcg atctgttgaa
     9481 ggtggttcta agcctcgtac ttgcgatggc atcggggcag gcacttgctg acctgccaat
     9541 tgttttagtg gatgaagctc gtcttcccta tgactactcc ccatccaact acgacatttc
     9601 tccaagcaac tacgacaact ccataagcaa ttacgacaat agtccatcaa attacgacaa
     9661 ctctgagagc aactacgata atagttcatc caattacgac aatagtcgca acggaaatcg
     9721 taggcttata tatagcgcaa atgggtctcg cactttcgcc ggctactacg tcattgccaa
     9781 caatgggaca acgaacttct tttccacatc tggcaaaagg atgttctaca ccccaaaagg
     9841 ggggcgcggc gtctatggcg gcaaagatgg gagcttctgc ggggcattgg tcgtcataaa
     9901 tggccaattt tcgcttgccc tgacagataa cggcctgaag atcatgtatc taagcaacta
     9961 gcctgctctc taataaaatg ttaggagctt ggctgccatt tttggggtga ggccgttcgc
    10021 ggccgagggg cgcagcccct ggggggatgg gaggcccgcg ttagcgggcc gggagggttc
    10081 gagaaggggg ggcacccccc ttcggcgtgc gcggtcacgc gccagggcgc agccctggtt
    10141 aaaaacaagg tttataaata ttggtttaaa agcaggttaa aagacaggtt agcggtggcc
    10201 gaaaaacggg cggaaaccct tgcaaatgct ggattttctg cctgtggaca gcccctcaaa
    10261 tgtcaatagg tgcgcccctc atctgtcagc actctgcccc tcaagtgtca aggatcgcgc
    10321 ccctcatctg tcagtagtcg cgcccctcaa gtgtcaatac cgcagggcac ttatccccag
    10381 gcttgtccac atcatctgtg ggaaactcgc gtaaaatcag gcgttttcgc cgatttgcga
    10441 ggctggccag ctccacgtcg ccggccgaaa tcgagcctgc ccctcatctg tcaacgccgc
    10501 gccgggtgag tcggcccctc aagtgtcaac gtccgcccct catctgtcag tgagggccaa
    10561 gttttccgcg aggtatccac aacgccggcg gccggccgcg gtgtctcgca cacggcttcg
    10621 acggcgtttc tggcgcgttt gcagggccat agacggccgc cagcccagcg gcgagggcaa
    10681 ccagcccggt gagcgtcgga aagggtcgac atcttgctgc gttcggatat tttcgtggag
    10741 ttcccgccac agacccggat tgaaggcgag atccagcaac tcgcgccaga tcatcctgtg
    10801 acggaacttt ggcgcgtgat gactggccag gacgtcggcc gaaagagcga caagcagatc
    10861 acgattttcg acagcgtcgg atttgcgatc gaggattttt cggcgctgcg ctacgtccgc
    10921 gaccgcgttg agggatcaag ccacagcagc ccactcgacc ttctagccga cccagacgag
    10981 ccaagggatc tttttggaat gctgctccgt cgtcaggctt tccgacgttt gggtggttga
    11041 acagaagtca ttatcgtacg gaatgccagc actcccgagg ggaaccctgt ggttggcatg
    11101 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgttattcta
    11161 ataaacgctc ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt
    11221 aaactgaagg cgggaaacga caatctgatc atgagcggag aattaaggga gtcacgttat
    11281 gacccccgcc gatgacgcgg gacaagccgt tttacgtttg gaactgacag aaccgcaacg
    11341 attgaaggag ccactcagcc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    11401 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    11461 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    11521 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    11581 attacgccaa gctatttagg tgacactata gaatactcaa gctatgcatc caacgcgttg
    11641 ggagctctcc catatcgacc tgcaggcggc cgctcgacga attaattcca atcccacaaa
    11701 aatctgagct taacagcaca gttgctcctc tcagagcaga atcgggtatt caacaccctc
    11761 atatcaacta ctacgttgtg tataacggtc cacatgccgg tatatacgat gactggggtt
    11821 gtacaaaggc ggcaacaaac ggcgttcccg gagttgcaca caagaaattt gccactatta
    11881 cagaggcaag agcagcagct gacgcgtaca caacaagtca gcaaacagac aggttgaact
    11941 tcatccccaa aggagaagct caactcaagc ccaagagctt tgctaaggcc ctaacaagcc
    12001 caccaaagca aaaagcccac tggctcacgc taggaaccaa aaggcccagc agtgatccag
    12061 ccccaaaaga gatctccttt gccccggaga ttacaatgga cgatttcctc tatctttacg
    12121 atctaggaag gaagttcgaa ggtgaaggtg acgacactat gttcaccact gataatgaga
    12181 aggttagcct cttcaatttc agaaagaatg ctgacccaca gatggttaga gaggcctacg
    12241 cagcaggtct catcaagacg atctacccga gtaacaatct ccaggagatc aaataccttc
    12301 ccaagaaggt taaagatgca gtcaaaagat tcaggactaa ttgcatcaag aacacagaga
    12361 aagacatatt tctcaagatc agaagtacta ttccagtatg gacgattcaa ggcttgcttc
    12421 ataaaccaag gcaagtaata gagattggag tctctaaaaa ggtagttcct actgaatcta
    12481 aggccatgca tggagtctaa gattcaaatc gaggatctaa cagaactcgc cgtgaagact
    12541 ggcgaacagt tcatacagag tcttttacga ctcaatgaca agaagaaaat cttcgtcaac
    12601 atggtggagc acgacactct ggtctactcc aaaaatgtca aagatacagt ctcagaagac
    12661 caaagggcta ttgagacttt tcaacaaagg ataatttcgg gaaacctcct cggattccat
    12721 tgcccagcta tctgtcactt catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa
    12781 tgccatcatt gcgataaagg aaaggctatc attcaagatc tctctgccga cagtggtccc
    12841 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
    12901 tcaaagcaag tggattgatg tgacatctcc actgacgtaa gggatgacgc acaatcccac
    12961 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc
    13021 tcgaggctag catggatctc gggccccaaa taatgatttt attttgactg atagtgacct
    13081 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
    13141 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa
    13201 aacagactac ataatactgt aaaacacaac atatccagtc actatgaatc aactacttag
    13261 atggtattag tgacctgtag tcgaccgaca gccttccaaa tgttcttcgg gtgatgctgc
    13321 caacttagtc gaccgacagc cttccaaatg ttcttctcaa acggaatcgt cgtatccagc
    13381 ctactcgcta ttgtcctcaa tgccgtatta aatcataaaa agaaataaga aaaagaggtg
    13441 cgagcctctt ttttgtgtga caaaataaaa acatctacct attcatatac gctagtgtca
    13501 tagtcctgaa aatcatctgc atcaagaaca atttcacaac tcttatactt ttctcttaca
    13561 agtcgttcgg cttcatctgg attttcagcc tctatactta ctaaacgtga taaagtttct
    13621 gtaatttcta ctgtatcgac ctgcagactg gctgtgtata agggagcctg acatttatat
    13681 tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc
    13741 cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca
    13801 gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag
    13861 acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg
    13921 tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat
    13981 ttcaccagtc cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct
    14041 cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat
    14101 tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct tgagcaactg
    14161 atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcacacc tctttttgac
    14221 atacttcggg tagtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
    14281 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
    14341 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta gtcgactaca ggtcactaat
    14401 accatctaag tagttgattc atagtgactg gatatgttgt gttttacagt attatgtagt
    14461 ctgtttttta tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg
    14521 ttcagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
    14581 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg cagctcctcg
    14641 aggaattcgg taccccaatt ggtaaggaaa taattatttt cttttttcct tttagtataa
    14701 aatagttaag tgatgttaat tagtatgatt ataataatat agttgttata attgtgaaaa
    14761 aataatttat aaatatattg tttacataaa caacatagta atgtaaaaaa atatgacaag
    14821 tgatgtgtaa gacgaagaag ataaaagttg agagtaagta tattattttt aatgaatttg
    14881 atcgaacatg taagatgata tacggccggt aagaggttcc aactttcacc ataatgaaat
    14941 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
    15001 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
    15061 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
    15121 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
    15181 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
    15241 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
    15301 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
    15361 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
    15421 gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
    15481 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
    15541 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtctgtg atggcttcca
    15601 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
    15661 atcgcgtgga tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt
    15721 ttgcggtata agaatatata ctgatatgtc gggcccataa tagtaattct agctggtttg
    15781 atgaattaaa tatcaatgat aaaatactat agtaaaaata agaataaata aattaaaata
    15841 atattttttt atgattaata gtttattata taattaaata tctataccat tactaaatat
    15901 tttagtttaa aagttaataa atattttgtt agaaattcca atctgcttgt aatttatcaa
    15961 taaacaaaat attaaataac aagctaaagt aacaaataat atcaaactaa tagaaacagt
    16021 aatctaatgt aacaaaacat aatctaatgc taatataaca aagcgcaaga tctatcattt
    16081 tatatagtat tattttcaat caacattctt attaatttct aaataatact tgtagtttta
    16141 ttaacttcta aatggattga ctattaatta aatgaattag tcgaacatga ataaacaagg
    16201 taacatgata gatcatgtca ttgtgttatc attgatctta catttggatt gattacagtt
    16261 gggaaattgg gttcgaaatc gataagcttg gatcctctag agagctgcag ctggatggca
    16321 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
    16381 ctttcttata atgccaactt tgtacaagaa agctgaacga gaaacgtaaa atgatataaa
    16441 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca
    16501 acatatccag tcactatgaa tcaactactt agatggtatt agtgacctgt agtcgactaa
    16561 gttggcagca tcacccgacg cactttgcgc cgaataaata cctgtgacgg aagatcactt
    16621 cgcagaataa ataaatcctg gtgtccctgt tgataccggg aagccctggg ccaacttttg
    16681 gcgaaaatga gacgttgatc ggcactaccc atttcacaac tcttatactt ttctcttaca
    16741 agtcgttcgg cttcatctgg attttcagcc tctatactta ctaaacgtga taaagtttct
    16801 gtaatttcta ctgtatcgac ctgcagactg gctgtgtata agggagcctg acatttatat
    16861 tccccagaac atcaggttaa tggcgttttt gatgtcattt tcgcggtggc tgagatcagc
    16921 cacttcttcc ccgataacgg agaccggcac actggccata tcggtggtca tcatgcgcca
    16981 gctttcatcc ccgatatgca ccaccgggta aagttcacgg gagactttat ctgacagcag
    17041 acgtgcactg gccaggggga tcaccatccg tcgcccgggc gtgtcaataa tatcactctg
    17101 tacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct taaactgcat
    17161 ttcaccagtc cctgttctcg tcagcaaaag agccgttcat ttcaataaac cgggcgacct
    17221 cagccatccc ttcctgattt tccgctttcc agcgttcggc acgcagacga cgggcttcat
    17281 tctgcatggt tgtgcttacc agaccggaga tattgacatc atatatgcct tgagcaactg
    17341 atagctgtcg ctgtcaactg tcactgtaat acgctgcttc atagcacacc tctttttgac
    17401 atacttctgt tcttgatgca gatgattttc aggactatga cactagcgta tatgaatagg
    17461 tagatgtttt tattttgtca cacaaaaaag aggctcgcac ctctttttct tatttctttt
    17521 tatgatttaa tacggcattg aggacaatag cgagtaggct ggatacgacg attccgtttg
    17581 agaagaacat ttggaaggct gtcggtcgac taagttggca gcatcacccg aagaacattt
    17641 ggaaggctgt cggtcgacta caggtcacta ataccatcta agtagttgat tcatagtgac
    17701 tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat
    17761 atattgatat ttatatcatt ttacgtttct cgttcagctt ttttgtacaa agttggcatt
    17821 ataaaaaagc attgctcatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
    17881 atcattattt ggggcccgag atccatgcta gctctagagt cctgctttaa tgagatatgc
    17941 gagacgccta tgatcgcatg atatttgctt tcaattctgt tgtgcacgtt gtaaaaaacc
    18001 tgagcatgtg tagctcagat ccttaccgcc ggtttcggtt cattctaatg aatatatcac
    18061 ccgttactat cgtattttta tgaataatat tctccgttca atttactgat tgtaccctac
    18121 tacttatatg tacaatatta aaatgaaaac aatatattgt gctgaatagg tttatagcga
    18181 catctatgat agagcgccac aataacaaac aattgcgttt tattattaca aatccaattt
    18241 taaaaaaagc ggcagaaccg gtcaaaccta aaagactgat tacataaatc ttattcaaat
    18301 ttcaaaaggc cccaggggct agtatctacg acacaccgag cggcgaacta ataacgttca
    18361 ctgaagggaa ctccggttcc ccgccggcgc gcatgggtga gattccttga agttgagtat
    18421 tggccgtccg ctctaccgaa agttacgggc accattcaac ccggtccagc acggcggccg
    18481 ggtaaccgac ttgctgcccc gagaattatg cagcattttt ttggtgtatg tgggccccaa
    18541 atgaagtgca ggtcaaacct tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat
    18601 tttgcgacaa catgtcgagg ctcagcagga cctgcaggca tgcaagctag cttactagtg
    18661 atgcatattc tatagtgtca cctaaatctg c
//
