LOCUS       V005532                 8321 bp    DNA     circular SYN 13-JAN-2022
DEFINITION  Exported.
ACCESSION   V005532
VERSION     V005532
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8321)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8321
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..54)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(62..78)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(86..116)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(131..152)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(440..1028)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1202..2059)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2060..2164)
                     /label="AmpR promoter"
     CDS             complement(2346..2993)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     primer_bind     3468..3484
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             complement(3530..4609)
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    5128..5144
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 tcgaggcctg caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
       61 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      841 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
      901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1141 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1201 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1261 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1321 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1381 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1441 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1501 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1561 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1621 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1681 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1741 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1801 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1861 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1921 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1981 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2041 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2101 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2161 cgcgcacatt tccccgaaaa gtgccacctg actgccgggc ctcttgcggg atcggcgagg
     2221 ctagttaccc ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg
     2281 tacctattaa tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca
     2341 tattataaaa gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat
     2401 cctgcatgat aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt
     2461 gaattacctt tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta
     2521 ttattgatat ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa
     2581 gcattttcag gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca
     2641 tcagaaaggt ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca
     2701 gagaatgttt tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa
     2761 cctaactctc cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt
     2821 gtcactaaga aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat
     2881 aaaacactaa tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga
     2941 ttaaatatct cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat
     3001 gcctcctaaa tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta
     3061 gaatcaatcc ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat
     3121 cccactttat ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac
     3181 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
     3241 ttttctttct tatcttgata ataagggtaa ctattgccga tcgtccattc cgacagcatc
     3301 gccagtcact atggcgtgct gctagcgcca ttcgccattc aggctgcgca actgttggga
     3361 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     3421 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     3481 cagtgaattc gagctcaggc cttaactcac attaattgcg ttgcgctcac tgcccgcttt
     3541 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     3601 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct
     3661 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
     3721 ccagcaggcg aaaatcctgt ttgatggtgg ttgacggcgg gatataacat gagctgtctt
     3781 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa
     3841 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
     3901 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
     3961 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
     4021 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
     4081 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt
     4141 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
     4201 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
     4261 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
     4321 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
     4381 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
     4441 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
     4501 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
     4561 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcatc aaaatcgtct
     4621 ccctccgttt gaatatttga ttgatcgtaa ccagatgaag cactctttcc actatcccta
     4681 cagtgttatg gcttgaacaa tcacgaaaca ataattggta cgtacgatct ttcagccgac
     4741 tcaaacatca aatcttacaa atgtagtctt tgaaagtatt acatatgtaa gatttaaatg
     4801 caaccgtttt ttcggaagga aatgatgacc tcgtttccac cggaattagc ttggtaccag
     4861 gccttacaca gcccagtcca gactattcgg cactgaaatt atgggtgaag tggtcaagac
     4921 ctcactaggc accttaaaaa tagcgcaccc tgaagaagat ttatttgagg tagcccttgc
     4981 ctacctagct tccaagaaag atatcctaac agcacaagag cggaaagatg ttttgttcta
     5041 catccagaac aacctctgct aaaattcctg aaaaattttg caaaaagttg ttgactttat
     5101 ctacaaggtg tggcataatg tgtggaattg tgagcggata acaattaagc ttaaggaggt
     5161 gaggatcctc tagagtcgac gtccccgggg cagcccgcct aatgagcggg cttttttcac
     5221 gtcacgcgtc catggagatc tttgtctgca actgaaaagt ttatacctta cctggaacaa
     5281 atggttgaaa catacgaggc taatatcggc ttattaggaa tagtccctgt actaataaaa
     5341 tcaggtggat cagttgatca gtatattttg gacgaagctc ggaaagaatt tggagatgac
     5401 ttgcttaatt ccacaattaa attaagggaa agaataaagc gatttgatgt tcaaggaatc
     5461 acggaagaag atactcatga taaagaagct ctaaaactat tcaataacct tacaatggaa
     5521 ttgatcgaaa gggtggaagg ttaatggtac gaaaattagg ggatctacct agaaagccac
     5581 aaggcgatag gtcaagctta aagaaccctt acatggatct tacagattct gaaagtaaag
     5641 aaacaacaga ggttaaacaa acagaaccaa aaagaaaaaa agcattgttg aaaacaatga
     5701 aagttgatgt ttcaatccat aataagatta aatcgctgca cgaaattctg gcagcatccg
     5761 aagggaattc atattactta gaggatacta ttgagagagc tattgataag atggttgaga
     5821 cattacctga gagccaaaaa actttttatg aatatgaatt aaaaaaaaga accaacaaag
     5881 gctgagacag actccaaacg agtctgtttt tttaaaaaaa atattaggag cattgaatat
     5941 atattagaga attaagaaag acatgggaat aaaaatattt taaatccagt aaaaatatga
     6001 taagattatt tcagaatatg aagaactctg tttgtttttg atgaaaaaac aaacaaaaaa
     6061 aatccaccta acggaatctc aatttaacta acagcggcca aactgagaag ttaaatttga
     6121 gaaggggaaa aggcggattt atacttgtat ttaactatct ccattttaac attttattaa
     6181 accccataca agtgaaaatc ctcttttaca ctgttccttt aggtgatcgc ggagggacat
     6241 tatgagtgaa gtaaacctaa aaggaaatac agatgaatta gtgtattatc gacagcaaac
     6301 cactggaaat aaaatcgcca ggaagagaat caaaaaaggg aaagaagaag tttattatgt
     6361 tgctgaaacg gaagagaaga tatggacaga agagcaaata aaaaactttt ctttagacaa
     6421 atttggtacg catatacctt acatagaagg tcattataca atcttaaata attacttctt
     6481 tgatttttgg ggctattttt taggtgctga aggaattgcg ctctatgctc acctaactcg
     6541 ttatgcatac ggcagcaaag acttttgctt tcctagtcta caaacaatcg ctaaaaaaat
     6601 ggacaagact cctgttacag ttagaggcta cttgaaactg cttgaaaggt acggttttat
     6661 ttggaaggta aacgtccgta ataaaaccaa ggataacaca gaggaatccc cgatttttaa
     6721 gattagacgt aaggttcctt tgctttcaga agaactttta aatggaaacc ctaatattga
     6781 aattccagat gacgaggaag cacatgtaaa gaaggcttta aaaaaggaaa aagagggtct
     6841 tccaaaggtt ttgaaaaaag agcacgatga atttgttaaa aaaatgatgg atgagtcaga
     6901 aacaattaat attccagagg ccttacaata tgacacaatg tatgaagata tactcagtaa
     6961 aggagaaatt cgaaaagaaa tcaaaaaaca aatacctaat cctacaacat cttttgagag
     7021 tatatcaatg acaactgaag aggaaaaagt cgacagtact ttaaaaagcg aaatgcaaaa
     7081 tcgtgtctct aagccttctt ttgatacctg gtttaaaaac actaagatca aaattgaaaa
     7141 taaaaattgt ttattacttg taccgagtga atttgcattt gaatggatta agaaaagata
     7201 tttagaaaca attaaaacag tccttgaaga agctggatat gttttcgaaa aaatcgaact
     7261 aagaaaagtg caataaactg ctgaagtatt tcagcagttt tttttattta gaaatagtga
     7321 aaaaaatata atcagggagg tatcaatatt taatgagtac tgatttaaat ttatttagac
     7381 tggaattaat aattaacacg tagactaatt aaaatttaat gagggataaa gaggatacaa
     7441 aaatattaat ttcaatccct attaaatttt aacaaggggg ggattaaaat ttaattagag
     7501 gtttatccac aagaaaagac cctaataaaa tttttactag ggttataaca ctgattaatt
     7561 tcttaatggg ggagggatta aaatttaatg acaaagaaaa caatctttta agaaaagctt
     7621 ttaaaagata ataataaaaa gagctttgcg attaagcaaa actctttact ttttcattga
     7681 cattatcaaa ttcatcgatt tcaaattgtt gttgtatcat aaagttaatt ctgttttgca
     7741 caaccttttc aggaatataa aacacatctg aggcttgttt tataaactca gggtcgctaa
     7801 agtcaatgta acgtagcata tgatatggta tagcttccac ccaagttagc ctttctgctt
     7861 cttctgaatg tttttcatat acttccatgg gtatctctaa atgattttcc tcatgtagca
     7921 aggtatgagc aaaaagttta tggaattgat agttcctctc tttttcttca acttttttat
     7981 ctaaaacaaa cactttaaca tctgagtcaa tgtaagcata agatgttttt ccagtcataa
     8041 tttcaatccc aaatctttta gacagaaatt ctggacgtaa atcttttggt gaaagaattt
     8101 ttttatgtag caatatatcc gatacagcac cttctaaaag cgttggtgaa tagggcattt
     8161 tacctatctc ctctcatttt gtggaataaa aatagtcata ttcgtccatc tacctatcct
     8221 attatcgaac agttgaactt tttaatcaag gatcagtcct ttttttcatt attcttaaac
     8281 tgtgctctta actttaacaa ctcgatttgt ttttccagat c
//
