LOCUS       V013413                 8089 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013413
VERSION     V013413
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8089)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..54)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(62..78)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(86..116)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(131..152)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(440..1028)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1202..2059)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2060..2164)
                     /label="AmpR promoter"
     CDS             complement(2346..2993)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     primer_bind     3468..3484
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 tcgaggcctg caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
       61 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      841 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
      901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1141 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1201 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1261 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1321 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1381 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1441 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1501 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1561 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1621 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1681 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1741 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1801 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1861 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1921 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1981 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2041 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2101 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2161 cgcgcacatt tccccgaaaa gtgccacctg actgccgggc ctcttgcggg atcggcgagg
     2221 ctagttaccc ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg
     2281 tacctattaa tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca
     2341 tattataaaa gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat
     2401 cctgcatgat aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt
     2461 gaattacctt tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta
     2521 ttattgatat ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa
     2581 gcattttcag gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca
     2641 tcagaaaggt ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca
     2701 gagaatgttt tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa
     2761 cctaactctc cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt
     2821 gtcactaaga aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat
     2881 aaaacactaa tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga
     2941 ttaaatatct cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat
     3001 gcctcctaaa tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta
     3061 gaatcaatcc ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat
     3121 cccactttat ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac
     3181 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
     3241 ttttctttct tatcttgata ataagggtaa ctattgccga tcgtccattc cgacagcatc
     3301 gccagtcact atggcgtgct gctagcgcca ttcgccattc aggctgcgca actgttggga
     3361 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     3421 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     3481 cagtgaattc gagctcctaa cttatagggg taacacttaa aaaagaatca ataacgatag
     3541 aaaccgctcc taaagcaggt gcattttttc ctaacgaaga aggcaatagt tcacatttat
     3601 tgtctaaatg agaatggact ctagaagaaa cttcgttttt aatcgtattt aaaacaatgg
     3661 gatgagattc aattatatga tttctcaaga taacagcttc tatatcaaat gtattaagga
     3721 tattggttaa tccaattccg atataaaagc caaagttttg aagtgcattt aacatttcta
     3781 catcattttt atttgcgcgt tccacaatct cttttcgaga aatattcttt tcttctttag
     3841 agagcgaagc cagtaacgct ttttcagaag catataattc ccaacagcct cgatttccac
     3901 agctgcattt gggtccatta aaatctatcg tcatatgacc catttcccca gaaaaaccct
     3961 gaacaccttt atacaattcg ttgttaataa caagtccagt tccaattccg atattaatac
     4021 tgatgtaaac gatgttttca tagttttttg tcataccaaa tactttttca ccgtatgctc
     4081 ctgcattagc ttcattttca acaaaaaccg gaacattaaa ctcactctca attaaaaact
     4141 gcaaatcttt gatattccaa tttaagttag gcatgaaaat aatttgctga tgacgatcta
     4201 caaggcctgg aacacaaatt cctattccga ctagaccata aggggactca ggcatatggg
     4261 ttacaaaacc atgaataagt gcaaataaaa tctcttttac ttcactagcg gaagaactag
     4321 acaagtcaga agtcttctcg agaataatat ttccttctaa gtcggttaga attccgttaa
     4381 gatagtcgac tcctatatca ataccaatcg agtagcctgc attcttatta aaaacaagca
     4441 ttacaggtct tctgccgcct ctagattgcc ctgccccaat ttcaaaaata aaatcttttt
     4501 caagcagtgt atttacttga gaggagacag tagacttgtt taatcctgta atctcagaga
     4561 gagttgccct ggagacaggg gagttcttca aaatttcatc taatattaat ttttgattca
     4621 ttttttttac taaagcttga tctgcaattt gaataataac cactcctttg tttatccacc
     4681 gaactaagtt ggtgtttttt gaagcttgaa ttagatattt aaaagtatca tatctaatat
     4741 tataactaaa ttttctaaaa aaaacattga aataaacatt tattttgtat atgatgagat
     4801 aaagttagtt tattggataa acaaactaac tcaattaaga tagttgatgg ataaacttgt
     4861 tcacttaaat caaaggggga aatgacaaat ggtccaaact agtgatatct aaaaatcaaa
     4921 gggggaaatg ggatcctcta gagtcgacgt ccccggggca gcccgcctaa tgagcgggct
     4981 tttttcacgt cacgcgtcca tggagatctt tgtctgcaac tgaaaagttt ataccttacc
     5041 tggaacaaat ggttgaaaca tacgaggcta atatcggctt attaggaata gtccctgtac
     5101 taataaaatc aggtggatca gttgatcagt atattttgga cgaagctcgg aaagaatttg
     5161 gagatgactt gcttaattcc acaattaaat taagggaaag aataaagcga tttgatgttc
     5221 aaggaatcac ggaagaagat actcatgata aagaagctct aaaactattc aataacctta
     5281 caatggaatt gatcgaaagg gtggaaggtt aatggtacga aaattagggg atctacctag
     5341 aaagccacaa ggcgataggt caagcttaaa gaacccttac atggatctta cagattctga
     5401 aagtaaagaa acaacagagg ttaaacaaac agaaccaaaa agaaaaaaag cattgttgaa
     5461 aacaatgaaa gttgatgttt caatccataa taagattaaa tcgctgcacg aaattctggc
     5521 agcatccgaa gggaattcat attacttaga ggatactatt gagagagcta ttgataagat
     5581 ggttgagaca ttacctgaga gccaaaaaac tttttatgaa tatgaattaa aaaaaagaac
     5641 caacaaaggc tgagacagac tccaaacgag tctgtttttt taaaaaaaat attaggagca
     5701 ttgaatatat attagagaat taagaaagac atgggaataa aaatatttta aatccagtaa
     5761 aaatatgata agattatttc agaatatgaa gaactctgtt tgtttttgat gaaaaaacaa
     5821 acaaaaaaaa tccacctaac ggaatctcaa tttaactaac agcggccaaa ctgagaagtt
     5881 aaatttgaga aggggaaaag gcggatttat acttgtattt aactatctcc attttaacat
     5941 tttattaaac cccatacaag tgaaaatcct cttttacact gttcctttag gtgatcgcgg
     6001 agggacatta tgagtgaagt aaacctaaaa ggaaatacag atgaattagt gtattatcga
     6061 cagcaaacca ctggaaataa aatcgccagg aagagaatca aaaaagggaa agaagaagtt
     6121 tattatgttg ctgaaacgga agagaagata tggacagaag agcaaataaa aaacttttct
     6181 ttagacaaat ttggtacgca tataccttac atagaaggtc attatacaat cttaaataat
     6241 tacttctttg atttttgggg ctatttttta ggtgctgaag gaattgcgct ctatgctcac
     6301 ctaactcgtt atgcatacgg cagcaaagac ttttgctttc ctagtctaca aacaatcgct
     6361 aaaaaaatgg acaagactcc tgttacagtt agaggctact tgaaactgct tgaaaggtac
     6421 ggttttattt ggaaggtaaa cgtccgtaat aaaaccaagg ataacacaga ggaatccccg
     6481 atttttaaga ttagacgtaa ggttcctttg ctttcagaag aacttttaaa tggaaaccct
     6541 aatattgaaa ttccagatga cgaggaagca catgtaaaga aggctttaaa aaaggaaaaa
     6601 gagggtcttc caaaggtttt gaaaaaagag cacgatgaat ttgttaaaaa aatgatggat
     6661 gagtcagaaa caattaatat tccagaggcc ttacaatatg acacaatgta tgaagatata
     6721 ctcagtaaag gagaaattcg aaaagaaatc aaaaaacaaa tacctaatcc tacaacatct
     6781 tttgagagta tatcaatgac aactgaagag gaaaaagtcg acagtacttt aaaaagcgaa
     6841 atgcaaaatc gtgtctctaa gccttctttt gatacctggt ttaaaaacac taagatcaaa
     6901 attgaaaata aaaattgttt attacttgta ccgagtgaat ttgcatttga atggattaag
     6961 aaaagatatt tagaaacaat taaaacagtc cttgaagaag ctggatatgt tttcgaaaaa
     7021 atcgaactaa gaaaagtgca ataaactgct gaagtatttc agcagttttt tttatttaga
     7081 aatagtgaaa aaaatataat cagggaggta tcaatattta atgagtactg atttaaattt
     7141 atttagactg gaattaataa ttaacacgta gactaattaa aatttaatga gggataaaga
     7201 ggatacaaaa atattaattt caatccctat taaattttaa caaggggggg attaaaattt
     7261 aattagaggt ttatccacaa gaaaagaccc taataaaatt tttactaggg ttataacact
     7321 gattaatttc ttaatggggg agggattaaa atttaatgac aaagaaaaca atcttttaag
     7381 aaaagctttt aaaagataat aataaaaaga gctttgcgat taagcaaaac tctttacttt
     7441 ttcattgaca ttatcaaatt catcgatttc aaattgttgt tgtatcataa agttaattct
     7501 gttttgcaca accttttcag gaatataaaa cacatctgag gcttgtttta taaactcagg
     7561 gtcgctaaag tcaatgtaac gtagcatatg atatggtata gcttccaccc aagttagcct
     7621 ttctgcttct tctgaatgtt tttcatatac ttccatgggt atctctaaat gattttcctc
     7681 atgtagcaag gtatgagcaa aaagtttatg gaattgatag ttcctctctt tttcttcaac
     7741 ttttttatct aaaacaaaca ctttaacatc tgagtcaatg taagcataag atgtttttcc
     7801 agtcataatt tcaatcccaa atcttttaga cagaaattct ggacgtaaat cttttggtga
     7861 aagaattttt ttatgtagca atatatccga tacagcacct tctaaaagcg ttggtgaata
     7921 gggcatttta cctatctcct ctcattttgt ggaataaaaa tagtcatatt cgtccatcta
     7981 cctatcctat tatcgaacag ttgaactttt taatcaagga tcagtccttt ttttcattat
     8041 tcttaaactg tgctcttaac tttaacaact cgatttgttt ttccagatc
//
