LOCUS       V014301                 9478 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014301
VERSION     V014301
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9478)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9478
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..54)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(62..78)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(86..116)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(131..152)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(440..1028)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1202..2059)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2060..2164)
                     /label="AmpR promoter"
     CDS             complement(2346..2993)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     primer_bind     3468..3484
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             4919..6316
                     /gene="sthA"
                     /label="Soluble pyridine nucleotide transhydrogenase"
                     /note="Soluble pyridine nucleotide transhydrogenase from
                     Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC
                     700720). Accession#: P66008"
ORIGIN
        1 tcgaggcctg caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
       61 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      841 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
      901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1141 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1201 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1261 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1321 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1381 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1441 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1501 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1561 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1621 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1681 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1741 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1801 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1861 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1921 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1981 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2041 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2101 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2161 cgcgcacatt tccccgaaaa gtgccacctg actgccgggc ctcttgcggg atcggcgagg
     2221 ctagttaccc ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg
     2281 tacctattaa tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca
     2341 tattataaaa gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat
     2401 cctgcatgat aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt
     2461 gaattacctt tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta
     2521 ttattgatat ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa
     2581 gcattttcag gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca
     2641 tcagaaaggt ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca
     2701 gagaatgttt tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa
     2761 cctaactctc cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt
     2821 gtcactaaga aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat
     2881 aaaacactaa tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga
     2941 ttaaatatct cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat
     3001 gcctcctaaa tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta
     3061 gaatcaatcc ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat
     3121 cccactttat ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac
     3181 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
     3241 ttttctttct tatcttgata ataagggtaa ctattgccga tcgtccattc cgacagcatc
     3301 gccagtcact atggcgtgct gctagcgcca ttcgccattc aggctgcgca actgttggga
     3361 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     3421 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     3481 cagtgaattc gagctcctaa cttatagggg taacacttaa aaaagaatca ataacgatag
     3541 aaaccgctcc taaagcaggt gcattttttc ctaacgaaga aggcaatagt tcacatttat
     3601 tgtctaaatg agaatggact ctagaagaaa cttcgttttt aatcgtattt aaaacaatgg
     3661 gatgagattc aattatatga tttctcaaga taacagcttc tatatcaaat gtattaagga
     3721 tattggttaa tccaattccg atataaaagc caaagttttg aagtgcattt aacatttcta
     3781 catcattttt atttgcgcgt tccacaatct cttttcgaga aatattcttt tcttctttag
     3841 agagcgaagc cagtaacgct ttttcagaag catataattc ccaacagcct cgatttccac
     3901 agctgcattt gggtccatta aaatctatcg tcatatgacc catttcccca gaaaaaccct
     3961 gaacaccttt atacaattcg ttgttaataa caagtccagt tccaattccg atattaatac
     4021 tgatgtaaac gatgttttca tagttttttg tcataccaaa tactttttca ccgtatgctc
     4081 ctgcattagc ttcattttca acaaaaaccg gaacattaaa ctcactctca attaaaaact
     4141 gcaaatcttt gatattccaa tttaagttag gcatgaaaat aatttgctga tgacgatcta
     4201 caaggcctgg aacacaaatt cctattccga ctagaccata aggggactca ggcatatggg
     4261 ttacaaaacc atgaataagt gcaaataaaa tctcttttac ttcactagcg gaagaactag
     4321 acaagtcaga agtcttctcg agaataatat ttccttctaa gtcggttaga attccgttaa
     4381 gatagtcgac tcctatatca ataccaatcg agtagcctgc attcttatta aaaacaagca
     4441 ttacaggtct tctgccgcct ctagattgcc ctgccccaat ttcaaaaata aaatcttttt
     4501 caagcagtgt atttacttga gaggagacag tagacttgtt taatcctgta atctcagaga
     4561 gagttgccct ggagacaggg gagttcttca aaatttcatc taatattaat ttttgattca
     4621 ttttttttac taaagcttga tctgcaattt gaataataac cactcctttg tttatccacc
     4681 gaactaagtt ggtgtttttt gaagcttgaa ttagatattt aaaagtatca tatctaatat
     4741 tataactaaa ttttctaaaa aaaacattga aataaacatt tattttgtat atgatgagat
     4801 aaagttagtt tattggataa acaaactaac tcaattaaga tagttgatgg ataaacttgt
     4861 tcacttaaat caaaggggga aatgacaaat ggtccaaact agtaagagga ggagaaatat
     4921 gccccacagc tgggactatg atgccgttgt cattggatct gggccgggtg gggaaggagc
     4981 agcgatggga ttggtcaaac agggcgcaag agtcgctgtc atagaacgtt atcataacgt
     5041 aggtggaggc tgcacacatt gggggaccat tccttcaaaa gcgctgcgcc atgctgtttc
     5101 aagaatcatt gaatttaatc aaaatccgct ttattcagat catagtaggc tgttgcggtc
     5161 cagcttcgca gacattctga atcacgctga caatgtgatc aatcagcaaa caagaatgag
     5221 acagggattt tatgagcgga accattgtga gattttgcaa ggaaatgcac attttattga
     5281 tgagcataca ctcgctttag aatgccacga tggcactgtt gaaaccctga cggccgaaaa
     5341 attcgtcatt gcatgcggaa gccggcctta tcacccaaac gatgttgatt tctctcatcc
     5401 gcgcatctac gacagtgaca gcatcctgag cctgcatcat gaaccacgac acgtcattat
     5461 atatggcgcc ggagtgatcg gatgtgaata cgcatcgatt ttccgcggca tggatgtgaa
     5521 agtggactta attaacacgc gtgaccgcct tttagctttt ttagatcagg aaatgtccga
     5581 ttcactttct taccactttt ggaattcggg tgtggtaata cggcacaatg aggaatatga
     5641 gaaaatcgaa ggctgtgatg acggtgtaat catgcatctt aaatcaggca agaaattgaa
     5701 ggctgattgt ttactctacg ctaacggaag gacaggcaat acagatagtc ttgcattgga
     5761 aaacataggg cttgaaacgg acagccgcgg ccagctgaag gttaactcaa tgtatcaaac
     5821 cgccttgcca catgtgtatg cggttggaga tgtaattggt tatccttctc tggcttccgc
     5881 agcctatgat caaggccgaa ttgcggcaca ggcgcttgtt aaaggagagg cgactgccca
     5941 ccttattgaa gatatcccga ctggcattta cacgatccct gaaatctcat ctgtagggaa
     6001 gacagaacag cagcttacgg cgatgaaagt gccgtacgaa gtagggcggg cgcaatttaa
     6061 acatttagcc agagctcaaa tcgtgggaat gaatgtcggc accttaaaga ttttatttca
     6121 tagagaaaca aaagagatct taggtattca ttgctttggc gagcgtgccg ctgagatcat
     6181 acatattggc caagcgatta tggaacaaaa aggcggcggg aacacaattg agtattttgt
     6241 caatacaaca tttaattatc cgacgatggc agaagcatac cgagttgctg cgctaaatgg
     6301 tctcaaccgt ctcttctaag gatcctctag agtcgacgtc cccggggcag cccgcctaat
     6361 gagcgggctt ttttcacgtc acgcgtccat ggagatcttt gtctgcaact gaaaagttta
     6421 taccttacct ggaacaaatg gttgaaacat acgaggctaa tatcggctta ttaggaatag
     6481 tccctgtact aataaaatca ggtggatcag ttgatcagta tattttggac gaagctcgga
     6541 aagaatttgg agatgacttg cttaattcca caattaaatt aagggaaaga ataaagcgat
     6601 ttgatgttca aggaatcacg gaagaagata ctcatgataa agaagctcta aaactattca
     6661 ataaccttac aatggaattg atcgaaaggg tggaaggtta atggtacgaa aattagggga
     6721 tctacctaga aagccacaag gcgataggtc aagcttaaag aacccttaca tggatcttac
     6781 agattctgaa agtaaagaaa caacagaggt taaacaaaca gaaccaaaaa gaaaaaaagc
     6841 attgttgaaa acaatgaaag ttgatgtttc aatccataat aagattaaat cgctgcacga
     6901 aattctggca gcatccgaag ggaattcata ttacttagag gatactattg agagagctat
     6961 tgataagatg gttgagacat tacctgagag ccaaaaaact ttttatgaat atgaattaaa
     7021 aaaaagaacc aacaaaggct gagacagact ccaaacgagt ctgttttttt aaaaaaaata
     7081 ttaggagcat tgaatatata ttagagaatt aagaaagaca tgggaataaa aatattttaa
     7141 atccagtaaa aatatgataa gattatttca gaatatgaag aactctgttt gtttttgatg
     7201 aaaaaacaaa caaaaaaaat ccacctaacg gaatctcaat ttaactaaca gcggccaaac
     7261 tgagaagtta aatttgagaa ggggaaaagg cggatttata cttgtattta actatctcca
     7321 ttttaacatt ttattaaacc ccatacaagt gaaaatcctc ttttacactg ttcctttagg
     7381 tgatcgcgga gggacattat gagtgaagta aacctaaaag gaaatacaga tgaattagtg
     7441 tattatcgac agcaaaccac tggaaataaa atcgccagga agagaatcaa aaaagggaaa
     7501 gaagaagttt attatgttgc tgaaacggaa gagaagatat ggacagaaga gcaaataaaa
     7561 aacttttctt tagacaaatt tggtacgcat ataccttaca tagaaggtca ttatacaatc
     7621 ttaaataatt acttctttga tttttggggc tattttttag gtgctgaagg aattgcgctc
     7681 tatgctcacc taactcgtta tgcatacggc agcaaagact tttgctttcc tagtctacaa
     7741 acaatcgcta aaaaaatgga caagactcct gttacagtta gaggctactt gaaactgctt
     7801 gaaaggtacg gttttatttg gaaggtaaac gtccgtaata aaaccaagga taacacagag
     7861 gaatccccga tttttaagat tagacgtaag gttcctttgc tttcagaaga acttttaaat
     7921 ggaaacccta atattgaaat tccagatgac gaggaagcac atgtaaagaa ggctttaaaa
     7981 aaggaaaaag agggtcttcc aaaggttttg aaaaaagagc acgatgaatt tgttaaaaaa
     8041 atgatggatg agtcagaaac aattaatatt ccagaggcct tacaatatga cacaatgtat
     8101 gaagatatac tcagtaaagg agaaattcga aaagaaatca aaaaacaaat acctaatcct
     8161 acaacatctt ttgagagtat atcaatgaca actgaagagg aaaaagtcga cagtacttta
     8221 aaaagcgaaa tgcaaaatcg tgtctctaag ccttcttttg atacctggtt taaaaacact
     8281 aagatcaaaa ttgaaaataa aaattgttta ttacttgtac cgagtgaatt tgcatttgaa
     8341 tggattaaga aaagatattt agaaacaatt aaaacagtcc ttgaagaagc tggatatgtt
     8401 ttcgaaaaaa tcgaactaag aaaagtgcaa taaactgctg aagtatttca gcagtttttt
     8461 ttatttagaa atagtgaaaa aaatataatc agggaggtat caatatttaa tgagtactga
     8521 tttaaattta tttagactgg aattaataat taacacgtag actaattaaa atttaatgag
     8581 ggataaagag gatacaaaaa tattaatttc aatccctatt aaattttaac aaggggggga
     8641 ttaaaattta attagaggtt tatccacaag aaaagaccct aataaaattt ttactagggt
     8701 tataacactg attaatttct taatggggga gggattaaaa tttaatgaca aagaaaacaa
     8761 tcttttaaga aaagctttta aaagataata ataaaaagag ctttgcgatt aagcaaaact
     8821 ctttactttt tcattgacat tatcaaattc atcgatttca aattgttgtt gtatcataaa
     8881 gttaattctg ttttgcacaa ccttttcagg aatataaaac acatctgagg cttgttttat
     8941 aaactcaggg tcgctaaagt caatgtaacg tagcatatga tatggtatag cttccaccca
     9001 agttagcctt tctgcttctt ctgaatgttt ttcatatact tccatgggta tctctaaatg
     9061 attttcctca tgtagcaagg tatgagcaaa aagtttatgg aattgatagt tcctctcttt
     9121 ttcttcaact tttttatcta aaacaaacac tttaacatct gagtcaatgt aagcataaga
     9181 tgtttttcca gtcataattt caatcccaaa tcttttagac agaaattctg gacgtaaatc
     9241 ttttggtgaa agaatttttt tatgtagcaa tatatccgat acagcacctt ctaaaagcgt
     9301 tggtgaatag ggcattttac ctatctcctc tcattttgtg gaataaaaat agtcatattc
     9361 gtccatctac ctatcctatt atcgaacagt tgaacttttt aatcaaggat cagtcctttt
     9421 tttcattatt cttaaactgt gctcttaact ttaacaactc gatttgtttt tccagatc
//
