LOCUS       V014020                 8914 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014020
VERSION     V014020
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8914)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8914
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(38..54)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(62..78)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(86..116)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(131..152)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(440..1028)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1202..2059)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(2060..2164)
                     /label="AmpR promoter"
     CDS             complement(2346..2993)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     primer_bind     3468..3484
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5735..5752
                     /label="6xHis"
                     /note="6xHis affinity tag"
ORIGIN
        1 tcgaggcctg caggcatgca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
       61 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
      121 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
      181 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      241 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
      301 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
      361 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
      421 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
      481 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      541 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
      601 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
      661 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
      721 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
      781 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
      841 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
      901 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
      961 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     1021 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     1081 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     1141 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     1201 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     1261 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     1321 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     1381 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     1441 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     1501 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     1561 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     1621 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     1681 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     1741 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     1801 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     1861 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     1921 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     1981 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     2041 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     2101 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     2161 cgcgcacatt tccccgaaaa gtgccacctg actgccgggc ctcttgcggg atcggcgagg
     2221 ctagttaccc ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg
     2281 tacctattaa tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca
     2341 tattataaaa gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat
     2401 cctgcatgat aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt
     2461 gaattacctt tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta
     2521 ttattgatat ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa
     2581 gcattttcag gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca
     2641 tcagaaaggt ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca
     2701 gagaatgttt tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa
     2761 cctaactctc cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt
     2821 gtcactaaga aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat
     2881 aaaacactaa tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga
     2941 ttaaatatct cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat
     3001 gcctcctaaa tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta
     3061 gaatcaatcc ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat
     3121 cccactttat ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac
     3181 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
     3241 ttttctttct tatcttgata ataagggtaa ctattgccga tcgtccattc cgacagcatc
     3301 gccagtcact atggcgtgct gctagcgcca ttcgccattc aggctgcgca actgttggga
     3361 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     3421 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     3481 cagtgaattc gagctcctaa cttatagggg taacacttaa aaaagaatca ataacgatag
     3541 aaaccgctcc taaagcaggt gcattttttc ctaacgaaga aggcaatagt tcacatttat
     3601 tgtctaaatg agaatggact ctagaagaaa cttcgttttt aatcgtattt aaaacaatgg
     3661 gatgagattc aattatatga tttctcaaga taacagcttc tatatcaaat gtattaagga
     3721 tattggttaa tccaattccg atataaaagc caaagttttg aagtgcattt aacatttcta
     3781 catcattttt atttgcgcgt tccacaatct cttttcgaga aatattcttt tcttctttag
     3841 agagcgaagc cagtaacgct ttttcagaag catataattc ccaacagcct cgatttccac
     3901 agctgcattt gggtccatta aaatctatcg tcatatgacc catttcccca gaaaaaccct
     3961 gaacaccttt atacaattcg ttgttaataa caagtccagt tccaattccg atattaatac
     4021 tgatgtaaac gatgttttca tagttttttg tcataccaaa tactttttca ccgtatgctc
     4081 ctgcattagc ttcattttca acaaaaaccg gaacattaaa ctcactctca attaaaaact
     4141 gcaaatcttt gatattccaa tttaagttag gcatgaaaat aatttgctga tgacgatcta
     4201 caaggcctgg aacacaaatt cctattccga ctagaccata aggggactca ggcatatggg
     4261 ttacaaaacc atgaataagt gcaaataaaa tctcttttac ttcactagcg gaagaactag
     4321 acaagtcaga agtcttctcg agaataatat ttccttctaa gtcggttaga attccgttaa
     4381 gatagtcgac tcctatatca ataccaatcg agtagcctgc attcttatta aaaacaagca
     4441 ttacaggtct tctgccgcct ctagattgcc ctgccccaat ttcaaaaata aaatcttttt
     4501 caagcagtgt atttacttga gaggagacag tagacttgtt taatcctgta atctcagaga
     4561 gagttgccct ggagacaggg gagttcttca aaatttcatc taatattaat ttttgattca
     4621 ttttttttac taaagcttga tctgcaattt gaataataac cactcctttg tttatccacc
     4681 gaactaagtt ggtgtttttt gaagcttgaa ttagatattt aaaagtatca tatctaatat
     4741 tataactaaa ttttctaaaa aaaacattga aataaacatt tattttgtat atgatgagat
     4801 aaagttagtt tattggataa acaaactaac tcaattaaga tagttgatgg ataaacttgt
     4861 tcacttaaat caaaggggga aatgacaaat ggtctaaact agtaagagga ggagaaatat
     4921 gtctgttcct aacaaacttc gtgataacgc tatggttatg cttaaagaaa catctcgtac
     4981 attcttcatc cctatctctc atcttcctgc tgaacttcaa aacgctgttg gctctgctta
     5041 cctttgcatg cgtgctatcg atgaaatcga agatcatcct gaacttgaag ctggcgttaa
     5101 atctcgtctt ctttacgcta tctctgatct tcttaaaaaa tctttcaacg aagatgaata
     5161 catggctctt gttggccctt accaagctga tcttcctgat gttacacttc aacttggcaa
     5221 ctggatcgct ctttgcccta caggcgttgc tgatcaagtt cttgatgcta catctatcat
     5281 ggctaaaggc atggctgatt gggttgaaaa agattggcat atccaaaacg aagctgatct
     5341 tgatgattac acattctacg ttgctggcct tgttggcgtt atgcttaacg atatctggaa
     5401 atggtacgat ggcacagaaa cagataaaga acttgctatc gctttcggcc gtggccttca
     5461 atctgttaac atccttcgta acacatctga agattctgaa cgtggcgttt ctttcttccc
     5521 taacaactgg tctcgtgaag atatgttcgc ttacgctcgt cgtaaccttt ctcttggcaa
     5581 aaaatacctt gaagatgtta aatctcttcc tatccttcat ttctgcaaaa tccctcttgc
     5641 tcttgctgat ggcacactta acgctcttat gaaaggcaaa gaaaaaatga cacgtgatga
     5701 tgttaacaaa acagttaacg aagttgttga tatgcatcat catcatcatc attaaggatc
     5761 ctctagagtc gacgtccccg gggcagcccg cctaatgagc gggctttttt cacgtcacgc
     5821 gtccatggag atctttgtct gcaactgaaa agtttatacc ttacctggaa caaatggttg
     5881 aaacatacga ggctaatatc ggcttattag gaatagtccc tgtactaata aaatcaggtg
     5941 gatcagttga tcagtatatt ttggacgaag ctcggaaaga atttggagat gacttgctta
     6001 attccacaat taaattaagg gaaagaataa agcgatttga tgttcaagga atcacggaag
     6061 aagatactca tgataaagaa gctctaaaac tattcaataa ccttacaatg gaattgatcg
     6121 aaagggtgga aggttaatgg tacgaaaatt aggggatcta cctagaaagc cacaaggcga
     6181 taggtcaagc ttaaagaacc cttacatgga tcttacagat tctgaaagta aagaaacaac
     6241 agaggttaaa caaacagaac caaaaagaaa aaaagcattg ttgaaaacaa tgaaagttga
     6301 tgtttcaatc cataataaga ttaaatcgct gcacgaaatt ctggcagcat ccgaagggaa
     6361 ttcatattac ttagaggata ctattgagag agctattgat aagatggttg agacattacc
     6421 tgagagccaa aaaacttttt atgaatatga attaaaaaaa agaaccaaca aaggctgaga
     6481 cagactccaa acgagtctgt ttttttaaaa aaaatattag gagcattgaa tatatattag
     6541 agaattaaga aagacatggg aataaaaata ttttaaatcc agtaaaaata tgataagatt
     6601 atttcagaat atgaagaact ctgtttgttt ttgatgaaaa aacaaacaaa aaaaatccac
     6661 ctaacggaat ctcaatttaa ctaacagcgg ccaaactgag aagttaaatt tgagaagggg
     6721 aaaaggcgga tttatacttg tatttaacta tctccatttt aacattttat taaaccccat
     6781 acaagtgaaa atcctctttt acactgttcc tttaggtgat cgcggaggga cattatgagt
     6841 gaagtaaacc taaaaggaaa tacagatgaa ttagtgtatt atcgacagca aaccactgga
     6901 aataaaatcg ccaggaagag aatcaaaaaa gggaaagaag aagtttatta tgttgctgaa
     6961 acggaagaga agatatggac agaagagcaa ataaaaaact tttctttaga caaatttggt
     7021 acgcatatac cttacataga aggtcattat acaatcttaa ataattactt ctttgatttt
     7081 tggggctatt ttttaggtgc tgaaggaatt gcgctctatg ctcacctaac tcgttatgca
     7141 tacggcagca aagacttttg ctttcctagt ctacaaacaa tcgctaaaaa aatggacaag
     7201 actcctgtta cagttagagg ctacttgaaa ctgcttgaaa ggtacggttt tatttggaag
     7261 gtaaacgtcc gtaataaaac caaggataac acagaggaat ccccgatttt taagattaga
     7321 cgtaaggttc ctttgctttc agaagaactt ttaaatggaa accctaatat tgaaattcca
     7381 gatgacgagg aagcacatgt aaagaaggct ttaaaaaagg aaaaagaggg tcttccaaag
     7441 gttttgaaaa aagagcacga tgaatttgtt aaaaaaatga tggatgagtc agaaacaatt
     7501 aatattccag aggccttaca atatgacaca atgtatgaag atatactcag taaaggagaa
     7561 attcgaaaag aaatcaaaaa acaaatacct aatcctacaa catcttttga gagtatatca
     7621 atgacaactg aagaggaaaa agtcgacagt actttaaaaa gcgaaatgca aaatcgtgtc
     7681 tctaagcctt cttttgatac ctggtttaaa aacactaaga tcaaaattga aaataaaaat
     7741 tgtttattac ttgtaccgag tgaatttgca tttgaatgga ttaagaaaag atatttagaa
     7801 acaattaaaa cagtccttga agaagctgga tatgttttcg aaaaaatcga actaagaaaa
     7861 gtgcaataaa ctgctgaagt atttcagcag ttttttttat ttagaaatag tgaaaaaaat
     7921 ataatcaggg aggtatcaat atttaatgag tactgattta aatttattta gactggaatt
     7981 aataattaac acgtagacta attaaaattt aatgagggat aaagaggata caaaaatatt
     8041 aatttcaatc cctattaaat tttaacaagg gggggattaa aatttaatta gaggtttatc
     8101 cacaagaaaa gaccctaata aaatttttac tagggttata acactgatta atttcttaat
     8161 gggggaggga ttaaaattta atgacaaaga aaacaatctt ttaagaaaag cttttaaaag
     8221 ataataataa aaagagcttt gcgattaagc aaaactcttt actttttcat tgacattatc
     8281 aaattcatcg atttcaaatt gttgttgtat cataaagtta attctgtttt gcacaacctt
     8341 ttcaggaata taaaacacat ctgaggcttg ttttataaac tcagggtcgc taaagtcaat
     8401 gtaacgtagc atatgatatg gtatagcttc cacccaagtt agcctttctg cttcttctga
     8461 atgtttttca tatacttcca tgggtatctc taaatgattt tcctcatgta gcaaggtatg
     8521 agcaaaaagt ttatggaatt gatagttcct ctctttttct tcaacttttt tatctaaaac
     8581 aaacacttta acatctgagt caatgtaagc ataagatgtt tttccagtca taatttcaat
     8641 cccaaatctt ttagacagaa attctggacg taaatctttt ggtgaaagaa tttttttatg
     8701 tagcaatata tccgatacag caccttctaa aagcgttggt gaatagggca ttttacctat
     8761 ctcctctcat tttgtggaat aaaaatagtc atattcgtcc atctacctat cctattatcg
     8821 aacagttgaa ctttttaatc aaggatcagt cctttttttc attattctta aactgtgctc
     8881 ttaactttaa caactcgatt tgtttttcca gatc
//
