LOCUS       40924_24303       10967 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHC94, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Chou H-H., Berthet J, Marx CJ.
  TITLE     Growth rate modulates the selective advantage of recurrent 
            transposition mutations arising from experimental populations grown 
            in a metal-limited environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10967)
  AUTHORS   Chou H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2008) Organismic and Evolutionary Biology, Harvard
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA
REFERENCE   3  (bases 1 to 10967)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10967)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2008) Organismic and Evolutionary Biology, Harvard 
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..712
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1981..2002
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2017..2047
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2055..2071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2079..2095
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2133..2864)
                     /codon_start=1
                     /gene="icuB"
                     /product="IcuB"
                     /label=icuB
                     /protein_id="ACR44986.1"
                     /translation="MRRTVILTALLAFTAPAAAHDLWLDPAGNGVQILYGHPHEPELPS
                     AGKLMSLTAYEPSGAVALNAKLQTGAMPALKAAHQGDALFAAAYDNGYWVRLSDGGYRN
                     ASKRMLPQADKSLWSVKFAKAVSGPTAPWDKIVGQPLEIVPLEEPAAASGQIRVRVLFE
                     GRPLDGASVVATDGVNFKSEADQARATTDADGVAVVSLRSAGPQVLGVAHRVSPSQTPA
                     LADADSYGATLAFTVTDPKSN"
     gene            complement(2133..2864)
                     /gene="icuB"
                     /label=icuB
     CDS             complement(2861..4975)
                     /codon_start=1
                     /gene="icuA"
                     /product="IcuA"
                     /label=icuA
                     /protein_id="ACR44987.1"
                     /translation="MSPLLTRLLASASALGVSTLPSHALDPTAVELEELQVTAQGGSAN
                     SVAAVAGPAANLKTQISPSVLALPGNSTVIDAAEIARLPVQSYGDLFRPVAGFDVSNYG
                     QGGVGYGIALRGFSDAEHGRDVAVFIDGVPINEVSSIHTPNYVDLNPLIPETIRSISIV
                     RGPFSVEAGDANLGGSIFIETKRAEPFSTVGASGGSFGTWRGLATFSQIGPGIQPFLAY
                     EGYGTGGYRENGDLGRYNAFNKVTIPLDLGSSLSLRVQMYGTGFGQPGYLERDRVRAGL
                     VSPRAATNITDGGGKTLQTFVANYVNGLPDDELSTLLYASHDVFSRFSDFGAGQRGQVE
                     ERETVGGRIRKVWTTSLFDVLPTQVLVGGDWRSDALDVNTGPSVARTFTARTLDLDLSQ
                     HNLAAYAQVQVKPAPWLKLTGGARFDQFFYDVRNNLDPALSPKVSPNVVSPKGGAALTP
                     FDWLELFANYGQGFRSPSASSELTNNPRLTPLTIESTEGGGRLVFDRFSLLGTVFFTDI
                     ANEVFQPAPGLPVQNLGKSRRDGFEIEAKVLAYREGLDRVALFANYSGVSARLLNGAPS
                     IFIPNVPAYLATVGAEFDVAAPAALLPGGRLTGAAYLSYIGAKPLTEDGVLHSSPYPRV
                     SGRIAYVHQSGWTTYGQATWYPGDRTSESIFNFGSSVSASAAEIVTSPQPRLTLLAGIT
                     SSFATGAPLR"
     gene            complement(2861..4975)
                     /gene="icuA"
                     /label=icuA
     promoter        complement(5003..5033)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     terminator      complement(5138..5165)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(5257..5343)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(5525..5541)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5986..6627)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKG"
     CDS             complement(7408..8220)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8895..10040)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(10312..10683)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(10716..10825)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1801 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1861 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1921 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1981 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2041 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2101 ttacgccaag cttgcatgcc taggctagcc gctcagttgg acttcgggtc ggtcacggtg
     2161 aaggcgagcg tcgcgccgta gctgtccgca tcggccagcg ccggcgtctg cgaggggctc
     2221 acgcggtgcg ccacgccgag cacctgggga ccggcactgc gcagcgagac gaccgccacg
     2281 ccgtccgcat ccgtcgtggc gcgggcctga tccgcctcgc tcttgaagtt cacgccgtcg
     2341 gtggcgacca cgctggcgcc gtcgagcgga cggccctcga acagcacccg cacccggatc
     2401 tggcccgaag ccgcggccgg ctcctccagc gggacgatct ccagcggttg gccgacgatc
     2461 ttgtcccacg gggccgtcgg gccggatacg gccttggcga acttcaccga ccagaggctc
     2521 ttgtcggcct gcggcagcat ccgcttgctg gcgttgcggt agccgccgtc ggacaagcgc
     2581 acccagtagc cgttgtcgta ggccgcggcg aacagcgcgt cgccctgatg cgcggctttg
     2641 agcgcgggca ttgcgccggt ctgcagcttg gcattcaggg ccaccgcgcc ggacggctcg
     2701 taggcggtca ggctcatcag cttgccagca ctcggcagtt ccggctcgtg gggatgcccg
     2761 tagaggatct gcacgccgtt gccggccggg tcgagccaga ggtcgtgggc ggcggccggt
     2821 gcggtgaagg cgagcagcgc ggtgaggatg acggttcgtc tcatcgaaga ggtgctcctg
     2881 ttgcaaagct ggaggtgatg ccggccagca gcgtcaggcg cggctgcggg ctggtgacga
     2941 tctcggcggc cgaggcagag accgacgagc cgaagttgaa gatgctctcg ctcgtgcggt
     3001 cgccgggata ccaggtggcc tgcccgtagg tcgtccagcc gctctggtgg acgtaggcga
     3061 tccggccgct gacgcgcgga tagggcgagg aatgcaatac gccgtcctcg gtcaacggct
     3121 tggcaccgat gtaggacagg taggccgcac cggtcaggcg cccgccgggc aacagggcgg
     3181 ccggcgcggc gacgtcgaat tcggcgccga cggtggcaag atacgccggc acgttgggga
     3241 tgaagatgga tggcgcgccg ttgagcagtc gggccgagac gcccgaatag ttggcgaaca
     3301 acgccacgcg gtcgaggcct tcacgatagg ccagcacctt cgcctcgatt tcgaagccgt
     3361 cgcggcggga cttgcccagg ttctgcacgg gcaggccggg tgcgggctgg aagacctcgt
     3421 tggcgatgtc ggtgaagaac accgtgccga ggagactgaa ccggtcgaat acgaggcggc
     3481 cgccgccctc cgtactctcg atggtcagcg gagtgaggcg gggattgttc gtcaattcgc
     3541 tcgaagcgga agggctgcgg aagccctggc cgtaattggc gaacagctcc agccagtcga
     3601 agggcgtgag cgccgccccg cccttggggg agaccacgtt ggggctgacc ttgggcgaca
     3661 gggccgggtc gagattgttg cgcacgtcgt agaagaactg gtcgaagcgg gcgccgccgg
     3721 tcagcttcag ccagggcgcc ggcttgacct gcacctgcgc gtaggccgcg aggttgtgct
     3781 gcgagagatc gagatcgagg gtgcgtgcgg tgaacgtgcg cgcgaccgag gggccggtgt
     3841 tcacgtcgag cgcgtcgctc cgccaatcgc cgccgacgag gacttgggtc ggcagcacgt
     3901 cgaacaggct cgtcgtccag accttgcgga tgcggccacc cacggtctcc cgttcctcga
     3961 cctgtccgcg ctggccggcg ccgaaatccg agaagcggct gaacacgtcg tggctggcgt
     4021 agagaagcgt cgacagctcg tcgtctggaa gcccgttgac gtagttggcc acgaaggtct
     4081 gcagggtctt gccgccgcca tcggtgatgt tggtcgcggc ccgcggtgag acgagaccgg
     4141 cgcgcacgcg gtcgcgctcc aggtagcccg gctgcccgaa ccccgtgccg tacatctgca
     4201 cccgcagcga caggctggag ccgagatcga gggggatggt gaccttgttg aaggcgttgt
     4261 agcgcccgag atcgccgttc tcgcgatagc ccccggtgcc gtagccctcg taggcgagga
     4321 agggctggat ccccgggccg atctggctga aggtggcaag cccccgccac gtaccgaacg
     4381 agccgcccga tgcgccgacg gtcgagaagg gctcggcccg cttggtctcg atgaagatcg
     4441 agccgccgag attggcgtcg cccgcttcga ccgagaacgg gccgcgcacg atgctgatgg
     4501 agcggatggt ctccgggatc agcggattga gatcgacgta gttcggggtg tggatcgagg
     4561 agacctcgtt gatcggcacc ccgtcgatga aaacggcgac gtcgcgaccg tgctcggcat
     4621 cgctgaagcc gcgcagggcg atgccgtatc cgaccccgcc ctgcccgtaa ttcgagacat
     4681 cgaagcctgc cacgggacgg aagaggtcgc cgtagctttg caccggcagg cgcgcgatct
     4741 ccgcggcatc gatcaccgtg ctgttgcccg gcagggccag gaccgatggt gagatctggg
     4801 tcttcaggtt cgcggccggt cccgcaaccg ccgcaacgga atttgcggaa ccgccctgcg
     4861 cggtgacctg tagttcctcc agctcaactg cggtcggatc gagcgcatgg ctcggaagcg
     4921 tactcacgcc gagtgcgctg gccgatgcga gcaggcgcgt cagaagcggc gacattcggg
     4981 gtctctccct ggtacctcca cacaacatac gagccggaag cataaagtgt aaagcctgga
     5041 attccctgct ttcctgatgc aaaaacgagg ctagtttacc gtatctgtgg ggggatggct
     5101 tgtagatatg acgacaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc
     5161 cttctgctta atttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg
     5221 ccgttgcttc gcaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt
     5281 caccgacaaa caacagataa aacgaaaggc ccagtctttc gactgagcct ttcgttttat
     5341 ttgatgcctg gcagttccct actctcgcat ggggagaccc cacactacca tcggcgctac
     5401 ggcgtttcac ttctgagttc ggcatggggt caggtgggac caccgcgcta ctgccgccag
     5461 gcaaattctg ttttatcaga ccgcttctgc gttctgattt aatctgtatc aggctgaaaa
     5521 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     5581 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     5641 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
     5701 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
     5761 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     5821 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
     5881 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
     5941 gcctattttt ataggttaat gtcatgataa taatggtttc ttagcaccct ttctcggtcc
     6001 ttcaacgttc ctgacaacga gcctcctttt cgccaatcca tcgacaatca ccgcgagtcc
     6061 ctgctcgaac gctgcgtccg gaccggcttc gtcgaaggcg tctatcgcgg cccgcaacag
     6121 cggcgagagc ggagcctgtt caacggtgcc gccgcgctcg ccggcatcgc tgtcgccggc
     6181 ctgctcctca agcacggccc caacagtgaa gtagctgatt gtcatcagcg cattgacggc
     6241 gtccccggcc gaaaaacccg cctcgcagag gaagcgaagc tgcgcgtcgg ccgtttccat
     6301 ctgcggtgcg cccggtcgcg tgccggcatg gatgcgcgcg ccatcgcggt aggcgagcag
     6361 cgcctgcctg aagctgcggg cattcccgat cagaaatgag cgccagtcgt cgtcggctct
     6421 cggcaccgaa tgcgtatgat tctccgccag catggcttcg gccagtgcgt cgagcagcgc
     6481 ccgcttgttc ctgaagtgcc agtaaagcgc cggctgctga acccccaacc gttccgccag
     6541 tttgcgtgtc gtcagaccgt ctacgccgac ctcgttcaac aggtccaggg cggcacggat
     6601 cactgtattc ggctgcaact ttgtcatgat tgacacttta tcactgataa acataatatg
     6661 tccaccaact tatcagtgat aaagaatccg cgcgttcaat cggaccagcg gaggctggtc
     6721 cggaggccag acgtgaaacc caacataccc ctgatcgtaa ttctgagcac tgtcgcgctc
     6781 gacgctgtcg gcatcggcct gattatgccg gtgctgccgg gcctcctgcg cgatctggtt
     6841 cactcgaacg acgtcaccgc ccactatggc attctgctgg cgctgtatgc gttggtgcaa
     6901 tttgcctgcg cacctgtgct gggcgcgctg tcggatcgtt tcgggcggcg gccaatcttg
     6961 ctcgtctcgc tggccggcgc cactgtcgac tacgccatca tggcgacagc gcctttcctt
     7021 tgggttctct atatcgggcg gatcgtggcc ggcatcaccg gggcgactgg ggcggtagcc
     7081 ggcgcttata ttgccgatga cctgcagggg ggggggggcg ctgaggtctg cctcgtgaag
     7141 aaggtgttgc tgactcatac caggcctgaa tcgccccatc atccagccag aaagtgaggg
     7201 agccacggtt gatgagagct ttgttgtagg tggaccagtt ggtgattttg aacttttgct
     7261 ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat ctgatccttc aactcagcaa
     7321 aagttcgatt tattcaacaa agccgccgtc ccgtcaagtc agcgtaatgc tctgccagtg
     7381 ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa
     7441 tttattcata tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg
     7501 agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc
     7561 gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag
     7621 tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc
     7681 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac
     7741 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa
     7801 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac
     7861 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat
     7921 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag
     7981 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac
     8041 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata
     8101 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc
     8161 atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa tatggctcat
     8221 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt
     8281 tttatcttgt gcaatgtaac atcagagatt ttgagacaca acgtggcttt cccccccccc
     8341 cctgcaggtc cgacacgggg atggatggcg ttcccgatca tggtcctgct tgcttcgggt
     8401 ggcatcggaa tgccggcgct gcaagcaatg ttgtccaggc aggtggatga ggaacgtcag
     8461 gggcagctgc aaggctcact ggcggcgctc accagcctga cctcgatcgt cggacccctc
     8521 ctcttcacgg cgatctatgc ggcttctata acaacgtgga acgggtgggc atggattgca
     8581 ggcgctgccc tctacttgct ctgcctgccg gcgctgcgtc gcgggctttg gagcggcgca
     8641 gggcaacgag ccgatcgctg atcgtggaaa cgataggcct atgccatgcg ggtcaaggcg
     8701 acttccggca agctatacgc gccctagaat tgtcaatttt aatcctctgt ttatcggcag
     8761 ttcgtagagc gcgccgtgcg tcccgagcga tactgagcga agcaagtgcg tcgagcagtg
     8821 cccgcttgtt cctgaaatgc cagtaaagcg ctggctgctg aacccccagc cggaactgac
     8881 cccacaaggc cctagcgttt gcaatgcacc aggtcatcat tgacccaggc gtgttccacc
     8941 aggccgctgc ctcgcaactc ttcgcaggct tcgccgacct gctcgcgcca cttcttcacg
     9001 cgggtggaat ccgatccgca catgaggcgg aaggtttcca gcttgagcgg gtacggctcc
     9061 cggtgcgagc tgaaatagtc gaacatccgt cgggccgtcg gcgacagctt gcggtacttc
     9121 tcccatatga atttcgtgta gtggtcgcca gcaaacagca cgacgatttc ctcgtcgatc
     9181 aggacctggc aacgggacgt tttcttgcca cggtccagga cgcggaagcg gtgcagcagc
     9241 gacaccgatt ccaggtgccc aacgcggtcg gacgtgaagc ccatcgccgt cgcctgtagg
     9301 cgcgacaggc attcctcggc cttcgtgtaa taccggccat tgatcgacca gcccaggtcc
     9361 tggcaaagct cgtagaacgt gaaggtgatc ggctcgccga taggggtgcg cttcgcgtac
     9421 tccaacacct gctgccacac cagttcgtca tcgtcggccc gcagctcgac gccggtgtag
     9481 gtgatcttca cgtccttgtt gacgtggaaa atgaccttgt tttgcagcgc ctcgcgcggg
     9541 attttcttgt tgcgcgtggt gaacagggca gagcgggccg tgtcgtttgg catcgctcgc
     9601 atcgtgtccg gccacggcgc aatatcgaac aaggaaagct gcatttcctt gatctgctgc
     9661 ttcgtgtgtt tcagcaacgc ggcctgcttg gcctcgctga cctgttttgc caggtcctcg
     9721 ccggcggttt ttcgcttctt ggtcgtcata gttcctcgcg tgtcgatggt catcgacttc
     9781 gccaaacctg ccgcctcctg ttcgagacga cgcgaacgct ccacggcggc cgatggcgcg
     9841 ggcagggcag ggggagccag ttgcacgctg tcgcgctcga tcttggccgt agcttgctgg
     9901 accatcgagc cgacggactg gaaggtttcg cggggcgcac gcatgacggt gcggcttgcg
     9961 atggtttcgg catcctcggc ggaaaacccc gcgtcgatca gttcttgcct gtatgccttc
    10021 cggtcaaacg tccgattcat tcaccctcct tgcgggattg ccccgactca cgccggggca
    10081 atgtgccctt attcctgatt tgacccgcct ggtgccttgg tgtccagata atccacctta
    10141 tcggcaatga agtcggtccc gtagaccgtc tggccgtcct tctcgtactt ggtattccga
    10201 atcttgccct gcacgaatac cagctccgcg aagtcgctct tcttgatgga gcgcatgggg
    10261 acgtgcttgg caatcacgcg caccccccgg ccgttttagc ggctaaaaaa gtcatggctc
    10321 tgccctcggg cggaccacgc ccatcatgac cttgccaagc tcgtcctgct tctcttcgat
    10381 cttcgccagc agggcgagga tcgtggcatc accgaaccgc gccgtgcgcg ggtcgtcggt
    10441 gagccagagt ttcagcaggc cgcccaggcg gcccaggtcg ccattgatgc gggccagctc
    10501 gcggacgtgc tcatagtcca cgacgcccgt gattttgtag ccctggccga cggccagcag
    10561 gtaggcctac aggctcatgc cggccgccgc cgccttttcc tcaatcgctc ttcgttcgtc
    10621 tggaaggcag tacaccttga taggtgggct gcccttcctg gttggcttgg tttcatcagc
    10681 catccgcttg ccctcatctg ttacgccggc ggtagccggc cagcctcgca gagcaggatt
    10741 cccgttgagc accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgcggg
    10801 tgggcctact tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac
    10861 acgaaccctt tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat
    10921 cgctataatg accccgaagc agggttatgc agcggaaaag atccgtc
//
