LOCUS       40924_24293       10246 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pHC92, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10246)
  AUTHORS   Chou H-H., Berthet J, Marx CJ.
  TITLE     Growth rate modulates the selective advantage of recurrent 
            transposition mutations arising from experimental populations grown 
            in a metal-limited environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10246)
  AUTHORS   Chou H-H.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2008) Organismic and Evolutionary Biology, Harvard
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA
REFERENCE   3  (bases 1 to 10246)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10246)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2008) Organismic and Evolutionary Biology, Harvard 
            University, 16 Divinity Avenue, Biolab 3079, Cambridge, MA 02138, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10246
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      3..712
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      1105..1693
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    1981..2002
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2017..2047
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2055..2071
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2079..2095
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(2140..4254)
                     /codon_start=1
                     /gene="icuA"
                     /product="IcuA"
                     /label=icuA
                     /protein_id="ACR44980.1"
                     /translation="MSPLLTRLLASASALGVSTLPSHALDPTAVELEELQVTAQGGSAN
                     SVAAVAGPAANLKTQISPSVLALPGNSTVIDAAEIARLPVQSYGDLFRPVAGFDVSNYG
                     QGGVGYGIALRGFSDAEHGRDVAVFIDGVPINEVSSIHTPNYVDLNPLIPETIRSISIV
                     RGPFSVEAGDANLGGSIFIETKRAEPFSTVGASGGSFGTWRGLATFSQIGPGIQPFLAY
                     EGYGTGGYRENGDLGRYNAFNKVTIPLDLGSSLSLRVQMYGTGFGQPGYLERDRVRAGL
                     VSPRAATNITDGGGKTLQTFVANYVNGLPDDELSTLLYASHDVFSRFSDFGAGQRGQVE
                     ERETVGGRIRKVWTTSLFDVLPTQVLVGGDWRSDALDVNTGPSVARTFTARTLDLDLSQ
                     HNLAAYAQVQVKPAPWLKLTGGARFDQFFYDVRNNLDPALSPKVSPNVVSPKGGAALTP
                     FDWLELFANYGQGFRSPSASSELTNNPRLTPLTIESTEGGGRLVFDRFSLLGTVFFTDI
                     ANEVFQPAPGLPVQNLGKSRRDGFEIEAKVLAYREGLDRVALFANYSGVSARLLNGAPS
                     IFIPNVPAYLATVGAEFDVAAPAALLPGGRLTGAAYLSYIGAKPLTEDGVLHSSPYPRV
                     SGRIAYVHQSGWTTYGQATWYPGDRTSESIFNFGSSVSASAAEIVTSPQPRLTLLAGIT
                     SSFATGAPLR"
     gene            complement(2140..4254)
                     /gene="icuA"
                     /label=icuA
     promoter        complement(4282..4312)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     terminator      complement(4417..4444)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(4536..4622)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     primer_bind     complement(4804..4820)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(5265..5906)
                     /codon_start=1
                     /label=TetR
                     /note="tetracycline resistance regulatory protein"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF
                     RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR
                     PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE
                     QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKG"
     CDS             complement(6687..7499)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8174..9319)
                     /codon_start=1
                     /label=trfA
                     /note="trans-acting replication protein that binds to and 
                     activates oriV"
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(9591..9962)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            complement(9995..10104)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
ORIGIN
        1 gaccctttcc gacgctcacc gggctggttg ccctcgccgc tgggctggcg gccgtctatg
       61 gccctgcaaa cgcgccagaa acgccgtcga agccgtgtgc gagacaccgc ggccgccggc
      121 gttgtggata cctcgcggaa aacttggccc tcactgacag atgaggggcg gacgttgaca
      181 cttgaggggc cgactcaccc ggcgcggcgt tgacagatga ggggcaggct cgatttcggc
      241 cggcgacgtg gagctggcca gcctcgcaaa tcggcgaaaa cgcctgattt tacgcgagtt
      301 tcccacagat gatgtggaca agcctgggga taagtgccct gcggtattga cacttgaggg
      361 gcgcgactac tgacagatga ggggcgcgat ccttgacact tgaggggcag agtgctgaca
      421 gatgaggggc gcacctattg acatttgagg ggctgtccac aggcagaaaa tccagcattt
      481 gcaagggttt ccgcccgttt ttcggccacc gctaacctgt cttttaacct gcttttaaac
      541 caatatttat aaaccttgtt tttaaccagg gctgcgccct gtgcgcgtga ccgcgcacgc
      601 cgaagggggg tgccccccct tctcgaaccc tcccggcccg ctaacgcggg cctcccatcc
      661 ccccaggggc tgcgcccctc ggccgcgaac ggcctcaccc caaaaatggc agccaagctg
      721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
      781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
      841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
      901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
      961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     1021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     1081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     1141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     1201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     1261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     1321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     1381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     1441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     1501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     1561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     1621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     1681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     1741 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     1801 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     1861 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1921 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1981 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     2041 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     2101 ttacgccaag cttgcatgcc taggctagca cggttcgtct catcgaagag gtgctcctgt
     2161 tgcaaagctg gaggtgatgc cggccagcag cgtcaggcgc ggctgcgggc tggtgacgat
     2221 ctcggcggcc gaggcagaga ccgacgagcc gaagttgaag atgctctcgc tcgtgcggtc
     2281 gccgggatac caggtggcct gcccgtaggt cgtccagccg ctctggtgga cgtaggcgat
     2341 ccggccgctg acgcgcggat agggcgagga atgcaatacg ccgtcctcgg tcaacggctt
     2401 ggcaccgatg taggacaggt aggccgcacc ggtcaggcgc ccgccgggca acagggcggc
     2461 cggcgcggcg acgtcgaatt cggcgccgac ggtggcaaga tacgccggca cgttggggat
     2521 gaagatggat ggcgcgccgt tgagcagtcg ggccgagacg cccgaatagt tggcgaacaa
     2581 cgccacgcgg tcgaggcctt cacgataggc cagcaccttc gcctcgattt cgaagccgtc
     2641 gcggcgggac ttgcccaggt tctgcacggg caggccgggt gcgggctgga agacctcgtt
     2701 ggcgatgtcg gtgaagaaca ccgtgccgag gagactgaac cggtcgaata cgaggcggcc
     2761 gccgccctcc gtactctcga tggtcagcgg agtgaggcgg ggattgttcg tcaattcgct
     2821 cgaagcggaa gggctgcgga agccctggcc gtaattggcg aacagctcca gccagtcgaa
     2881 gggcgtgagc gccgccccgc ccttggggga gaccacgttg gggctgacct tgggcgacag
     2941 ggccgggtcg agattgttgc gcacgtcgta gaagaactgg tcgaagcggg cgccgccggt
     3001 cagcttcagc cagggcgccg gcttgacctg cacctgcgcg taggccgcga ggttgtgctg
     3061 cgagagatcg agatcgaggg tgcgtgcggt gaacgtgcgc gcgaccgagg ggccggtgtt
     3121 cacgtcgagc gcgtcgctcc gccaatcgcc gccgacgagg acttgggtcg gcagcacgtc
     3181 gaacaggctc gtcgtccaga ccttgcggat gcggccaccc acggtctccc gttcctcgac
     3241 ctgtccgcgc tggccggcgc cgaaatccga gaagcggctg aacacgtcgt ggctggcgta
     3301 gagaagcgtc gacagctcgt cgtctggaag cccgttgacg tagttggcca cgaaggtctg
     3361 cagggtcttg ccgccgccat cggtgatgtt ggtcgcggcc cgcggtgaga cgagaccggc
     3421 gcgcacgcgg tcgcgctcca ggtagcccgg ctgcccgaac cccgtgccgt acatctgcac
     3481 ccgcagcgac aggctggagc cgagatcgag ggggatggtg accttgttga aggcgttgta
     3541 gcgcccgaga tcgccgttct cgcgatagcc cccggtgccg tagccctcgt aggcgaggaa
     3601 gggctggatc cccgggccga tctggctgaa ggtggcaagc ccccgccacg taccgaacga
     3661 gccgcccgat gcgccgacgg tcgagaaggg ctcggcccgc ttggtctcga tgaagatcga
     3721 gccgccgaga ttggcgtcgc ccgcttcgac cgagaacggg ccgcgcacga tgctgatgga
     3781 gcggatggtc tccgggatca gcggattgag atcgacgtag ttcggggtgt ggatcgagga
     3841 gacctcgttg atcggcaccc cgtcgatgaa aacggcgacg tcgcgaccgt gctcggcatc
     3901 gctgaagccg cgcagggcga tgccgtatcc gaccccgccc tgcccgtaat tcgagacatc
     3961 gaagcctgcc acgggacgga agaggtcgcc gtagctttgc accggcaggc gcgcgatctc
     4021 cgcggcatcg atcaccgtgc tgttgcccgg cagggccagg accgatggtg agatctgggt
     4081 cttcaggttc gcggccggtc ccgcaaccgc cgcaacggaa tttgcggaac cgccctgcgc
     4141 ggtgacctgt agttcctcca gctcaactgc ggtcggatcg agcgcatggc tcggaagcgt
     4201 actcacgccg agtgcgctgg ccgatgcgag caggcgcgtc agaagcggcg acattcgggg
     4261 tctctccctg gtacctccac acaacatacg agccggaagc ataaagtgta aagcctggaa
     4321 ttccctgctt tcctgatgca aaaacgaggc tagtttaccg tatctgtggg gggatggctt
     4381 gtagatatga cgacaggaag agtttgtaga aacgcaaaaa ggccatccgt caggatggcc
     4441 ttctgcttaa tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca ccctccgggc
     4501 cgttgcttcg caacgttcaa atccgctccc ggcggatttg tcctactcag gagagcgttc
     4561 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
     4621 tgatgcctgg cagttcccta ctctcgcatg gggagacccc acactaccat cggcgctacg
     4681 gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac tgccgccagg
     4741 caaattctgt tttatcagac cgcttctgcg ttctgattta atctgtatca ggctgaaaaa
     4801 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     4861 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     4921 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct
     4981 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
     5041 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
     5101 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     5161 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
     5221 cctattttta taggttaatg tcatgataat aatggtttct tagcaccctt tctcggtcct
     5281 tcaacgttcc tgacaacgag cctccttttc gccaatccat cgacaatcac cgcgagtccc
     5341 tgctcgaacg ctgcgtccgg accggcttcg tcgaaggcgt ctatcgcggc ccgcaacagc
     5401 ggcgagagcg gagcctgttc aacggtgccg ccgcgctcgc cggcatcgct gtcgccggcc
     5461 tgctcctcaa gcacggcccc aacagtgaag tagctgattg tcatcagcgc attgacggcg
     5521 tccccggccg aaaaacccgc ctcgcagagg aagcgaagct gcgcgtcggc cgtttccatc
     5581 tgcggtgcgc ccggtcgcgt gccggcatgg atgcgcgcgc catcgcggta ggcgagcagc
     5641 gcctgcctga agctgcgggc attcccgatc agaaatgagc gccagtcgtc gtcggctctc
     5701 ggcaccgaat gcgtatgatt ctccgccagc atggcttcgg ccagtgcgtc gagcagcgcc
     5761 cgcttgttcc tgaagtgcca gtaaagcgcc ggctgctgaa cccccaaccg ttccgccagt
     5821 ttgcgtgtcg tcagaccgtc tacgccgacc tcgttcaaca ggtccagggc ggcacggatc
     5881 actgtattcg gctgcaactt tgtcatgatt gacactttat cactgataaa cataatatgt
     5941 ccaccaactt atcagtgata aagaatccgc gcgttcaatc ggaccagcgg aggctggtcc
     6001 ggaggccaga cgtgaaaccc aacatacccc tgatcgtaat tctgagcact gtcgcgctcg
     6061 acgctgtcgg catcggcctg attatgccgg tgctgccggg cctcctgcgc gatctggttc
     6121 actcgaacga cgtcaccgcc cactatggca ttctgctggc gctgtatgcg ttggtgcaat
     6181 ttgcctgcgc acctgtgctg ggcgcgctgt cggatcgttt cgggcggcgg ccaatcttgc
     6241 tcgtctcgct ggccggcgcc actgtcgact acgccatcat ggcgacagcg cctttccttt
     6301 gggttctcta tatcgggcgg atcgtggccg gcatcaccgg ggcgactggg gcggtagccg
     6361 gcgcttatat tgccgatgac ctgcaggggg gggggggcgc tgaggtctgc ctcgtgaaga
     6421 aggtgttgct gactcatacc aggcctgaat cgccccatca tccagccaga aagtgaggga
     6481 gccacggttg atgagagctt tgttgtaggt ggaccagttg gtgattttga acttttgctt
     6541 tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc tgatccttca actcagcaaa
     6601 agttcgattt attcaacaaa gccgccgtcc cgtcaagtca gcgtaatgct ctgccagtgt
     6661 tacaaccaat taaccaattc tgattagaaa aactcatcga gcatcaaatg aaactgcaat
     6721 ttattcatat caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga
     6781 gaaaactcac cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg
     6841 actcgtccaa catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt
     6901 gagaaatcac catgagtgac gactgaatcc ggtgagaatg gcaaaagctt atgcatttct
     6961 ttccagactt gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc
     7021 aaaccgttat tcattcgtga ttgcgcctga gcgagacgaa atacgcgatc gctgttaaaa
     7081 ggacaattac aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca
     7141 atattttcac ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc
     7201 gcagtggtga gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga
     7261 ggcataaatt ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg
     7321 ctacctttgc catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag
     7381 attgtcgcac ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca
     7441 tccatgttgg aatttaatcg cggcctcgag caagacgttt cccgttgaat atggctcata
     7501 acaccccttg tattactgtt tatgtaagca gacagtttta ttgttcatga tgatatattt
     7561 ttatcttgtg caatgtaaca tcagagattt tgagacacaa cgtggctttc cccccccccc
     7621 ctgcaggtcc gacacgggga tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg
     7681 gcatcggaat gccggcgctg caagcaatgt tgtccaggca ggtggatgag gaacgtcagg
     7741 ggcagctgca aggctcactg gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc
     7801 tcttcacggc gatctatgcg gcttctataa caacgtggaa cgggtgggca tggattgcag
     7861 gcgctgccct ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag
     7921 ggcaacgagc cgatcgctga tcgtggaaac gataggccta tgccatgcgg gtcaaggcga
     7981 cttccggcaa gctatacgcg ccctagaatt gtcaatttta atcctctgtt tatcggcagt
     8041 tcgtagagcg cgccgtgcgt cccgagcgat actgagcgaa gcaagtgcgt cgagcagtgc
     8101 ccgcttgttc ctgaaatgcc agtaaagcgc tggctgctga acccccagcc ggaactgacc
     8161 ccacaaggcc ctagcgtttg caatgcacca ggtcatcatt gacccaggcg tgttccacca
     8221 ggccgctgcc tcgcaactct tcgcaggctt cgccgacctg ctcgcgccac ttcttcacgc
     8281 gggtggaatc cgatccgcac atgaggcgga aggtttccag cttgagcggg tacggctccc
     8341 ggtgcgagct gaaatagtcg aacatccgtc gggccgtcgg cgacagcttg cggtacttct
     8401 cccatatgaa tttcgtgtag tggtcgccag caaacagcac gacgatttcc tcgtcgatca
     8461 ggacctggca acgggacgtt ttcttgccac ggtccaggac gcggaagcgg tgcagcagcg
     8521 acaccgattc caggtgccca acgcggtcgg acgtgaagcc catcgccgtc gcctgtaggc
     8581 gcgacaggca ttcctcggcc ttcgtgtaat accggccatt gatcgaccag cccaggtcct
     8641 ggcaaagctc gtagaacgtg aaggtgatcg gctcgccgat aggggtgcgc ttcgcgtact
     8701 ccaacacctg ctgccacacc agttcgtcat cgtcggcccg cagctcgacg ccggtgtagg
     8761 tgatcttcac gtccttgttg acgtggaaaa tgaccttgtt ttgcagcgcc tcgcgcggga
     8821 ttttcttgtt gcgcgtggtg aacagggcag agcgggccgt gtcgtttggc atcgctcgca
     8881 tcgtgtccgg ccacggcgca atatcgaaca aggaaagctg catttccttg atctgctgct
     8941 tcgtgtgttt cagcaacgcg gcctgcttgg cctcgctgac ctgttttgcc aggtcctcgc
     9001 cggcggtttt tcgcttcttg gtcgtcatag ttcctcgcgt gtcgatggtc atcgacttcg
     9061 ccaaacctgc cgcctcctgt tcgagacgac gcgaacgctc cacggcggcc gatggcgcgg
     9121 gcagggcagg gggagccagt tgcacgctgt cgcgctcgat cttggccgta gcttgctgga
     9181 ccatcgagcc gacggactgg aaggtttcgc ggggcgcacg catgacggtg cggcttgcga
     9241 tggtttcggc atcctcggcg gaaaaccccg cgtcgatcag ttcttgcctg tatgccttcc
     9301 ggtcaaacgt ccgattcatt caccctcctt gcgggattgc cccgactcac gccggggcaa
     9361 tgtgccctta ttcctgattt gacccgcctg gtgccttggt gtccagataa tccaccttat
     9421 cggcaatgaa gtcggtcccg tagaccgtct ggccgtcctt ctcgtacttg gtattccgaa
     9481 tcttgccctg cacgaatacc agctccgcga agtcgctctt cttgatggag cgcatgggga
     9541 cgtgcttggc aatcacgcgc accccccggc cgttttagcg gctaaaaaag tcatggctct
     9601 gccctcgggc ggaccacgcc catcatgacc ttgccaagct cgtcctgctt ctcttcgatc
     9661 ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg ccgtgcgcgg gtcgtcggtg
     9721 agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc cattgatgcg ggccagctcg
     9781 cggacgtgct catagtccac gacgcccgtg attttgtagc cctggccgac ggccagcagg
     9841 taggcctaca ggctcatgcc ggccgccgcc gccttttcct caatcgctct tcgttcgtct
     9901 ggaaggcagt acaccttgat aggtgggctg cccttcctgg ttggcttggt ttcatcagcc
     9961 atccgcttgc cctcatctgt tacgccggcg gtagccggcc agcctcgcag agcaggattc
    10021 ccgttgagca ccgccaggtg cgaataaggg acagtgaaga aggaacaccc gctcgcgggt
    10081 gggcctactt cacctatcct gcccggctga cgccgttgga tacaccaagg aaagtctaca
    10141 cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg atggatatac cgaaaaaatc
    10201 gctataatga ccccgaagca gggttatgca gcggaaaaga tccgtc
//
